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  1. Article ; Online: 2021 Reviews Issue.

    Kennedy, Robert T

    Analytical chemistry

    2020  Volume 93, Issue 1, Page(s) 1–2

    Language English
    Publishing date 2020-12-16
    Publishing country United States
    Document type Editorial
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.0c05049
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4033: Show issues Location:
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  2. Article ; Online: 2020 Reviews Issue.

    Kennedy, Robert T

    Analytical chemistry

    2020  Volume 92, Issue 1, Page(s) 1

    Language English
    Publishing date 2020-01-07
    Publishing country United States
    Document type Editorial
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.9b05664
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Evaluation of Surface Treatments of PDMS Microfluidic Devices for Improving Small-Molecule Recovery with Application to Monitoring Metabolites Secreted from Islets of Langerhans.

    Lenhart, Ashley E / Kennedy, Robert T

    ACS measurement science au

    2023  Volume 3, Issue 5, Page(s) 380–389

    Abstract: Microfluidic devices are becoming an important tool for bioanalysis with applications including studying cell secretion, cell growth, and drug delivery. Small molecules such as drugs, cell products, or nutrients may partition into polydimethylsiloxane ( ... ...

    Abstract Microfluidic devices are becoming an important tool for bioanalysis with applications including studying cell secretion, cell growth, and drug delivery. Small molecules such as drugs, cell products, or nutrients may partition into polydimethylsiloxane (PDMS), a commonly used material for microfluidic devices, potentially leading to poor recovery or inaccurate delivery of such chemicals. To decrease small-molecule partitioning, surface and bulk PDMS treatments have been developed; however, these have been tested on few analytes, or their biocompatibility are unknown. Studies often focus on one analyte, whereas a diversity of chemicals are of interest and possibly affected. In this study, 11 device treatments are tested and applied to 21 biologically relevant small molecules with a variety of chemical structures. Device treatments are characterized using water contact angle measurements and evaluated by measuring recovery of the 21 target analytes using liquid chromatography-mass spectrometry. 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide (polybrene), a positively charged polymer, produced the least hydrophilic surface and was found to provide the best recovery with most of the analytes having >50% recovery and up to 92% recovery; however, recovery varied by analyte highlighting the importance of analyte diversity rather than targeting a single analyte in evaluating treatments. A polybrene-treated device was applied to investigate secretion from pancreatic islets, which are micro-organs involved in glucose homeostasis and diabetes. Islets secrete small molecules that have been shown to modulate the secretion of islets' main functional products, glucose-regulating hormones. The polybrene treatment enabled the detection of 20 target analytes from islets-on-chip during isosmotic and hypo-osmotic glucose perfusions and resulted in detection of more significant secretion changes compared to untreated PDMS.
    Language English
    Publishing date 2023-08-24
    Publishing country United States
    Document type Journal Article
    ISSN 2694-250X
    ISSN (online) 2694-250X
    DOI 10.1021/acsmeasuresciau.3c00025
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Preparation of high-efficiency HILIC capillary columns utilizing slurry packing at 2100 bar.

    Anderson, Brady G / Hancock, Tate A / Kennedy, Robert T

    Journal of chromatography. A

    2024  Volume 1722, Page(s) 464856

    Abstract: Complex mixture analysis requires high-efficiency chromatography columns. Although reversed phase liquid chromatography (RPLC) is the dominant approach for such mixtures, hydrophilic interaction liquid chromatography (HILIC) is an important complement to ...

    Abstract Complex mixture analysis requires high-efficiency chromatography columns. Although reversed phase liquid chromatography (RPLC) is the dominant approach for such mixtures, hydrophilic interaction liquid chromatography (HILIC) is an important complement to RPLC by enabling the separation of polar compounds. Chromatography theory predicts that small particles and long columns will yield high efficiency; however, little work has been done to prepare HILIC columns longer than 25 cm packed with sub-2 μm particles. In this work, we tested the slurry packing of 75 cm long HILIC columns with 1.7 μm bridged-ethyl-hybrid amide HILIC particles at 2,100 bar (30,000 PSI). Acetonitrile, methanol, acetone, and water were tested as slurry solvents, with acetonitrile providing the best columns. Slurry concentrations of 50-200 mg/mL were assessed, and while 50-150 mg/mL provided comparable results, the 150 mg/mL columns provided the shortest packing times (9 min). Columns prepared using 150 mg/mL slurries in acetonitrile yielded a reduced minimum plate height (h
    MeSH term(s) Hydrophobic and Hydrophilic Interactions ; Acetonitriles/chemistry ; Chromatography, Liquid/methods ; Chromatography, Reverse-Phase/methods ; Chromatography, Reverse-Phase/instrumentation ; Methanol/chemistry ; Solvents/chemistry ; Acetone/chemistry ; Particle Size ; Pressure ; Water/chemistry ; Benzenesulfonates
    Chemical Substances Acetonitriles ; acetonitrile (Z072SB282N) ; Methanol (Y4S76JWI15) ; Solvents ; Acetone (1364PS73AF) ; Water (059QF0KO0R) ; 4-toluenesulfonic acid (QGV5ZG5741) ; Benzenesulfonates
    Language English
    Publishing date 2024-03-30
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1171488-8
    ISSN 1873-3778 ; 0021-9673
    ISSN (online) 1873-3778
    ISSN 0021-9673
    DOI 10.1016/j.chroma.2024.464856
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 813: Show issues Location:
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  5. Article ; Online: The 2019 Reviews Issue.

    Kennedy, Robert T

    Analytical chemistry

    2019  Volume 91, Issue 1, Page(s) 1

    Language English
    Publishing date 2019-05-24
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.8b05701
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 4033: Show issues Location:
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  6. Book ; Conference proceedings: 21st International Symposium on Microscale Bioseparations

    Kennedy, Robert T.

    Vancouver, Canada, 14 - 18 January 2007

    (Journal of chromatography : A ; 1162,2 = Symposium issue)

    2007  

    Event/congress International Symposium on Microscale Bioseparations (21, 2007, VancouverBritishColumbia)
    Author's details guest ed.: R. T. Kennedy
    Series title Journal of chromatography : A ; 1162,2 = Symposium issue
    Journal of chromatography
    Journal of chromatography ; A
    Collection Journal of chromatography
    Journal of chromatography ; A
    Language English
    Publisher Elsevier
    Publishing place Amsterdam u.a.
    Publishing country Netherlands
    Document type Book ; Conference proceedings
    HBZ-ID HT015261105
    Database Catalogue ZB MED Medicine, Health

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 813 -1162- not available for loan Zeitschriften-Lesesaal

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  7. Article ; Online: Cyclic Ion Mobility-Mass Spectrometry and Tandem Collision Induced Unfolding for Quantification of Elusive Protein Biomarkers.

    Makey, Devin M / Gadkari, Varun V / Kennedy, Robert T / Ruotolo, Brandon T

    Analytical chemistry

    2024  Volume 96, Issue 15, Page(s) 6021–6029

    Abstract: Sensitive analytical techniques that are capable of detecting and quantifying disease-associated biomolecules are indispensable in our efforts to understand disease mechanisms and guide therapeutic intervention through early detection, accurate diagnosis, ...

    Abstract Sensitive analytical techniques that are capable of detecting and quantifying disease-associated biomolecules are indispensable in our efforts to understand disease mechanisms and guide therapeutic intervention through early detection, accurate diagnosis, and effective monitoring of disease. Parkinson's Disease (PD), for example, is one of the most prominent neurodegenerative disorders in the world, but the diagnosis of PD has primarily been based on the observation of clinical symptoms. The protein α-synuclein (α-syn) has emerged as a promising biomarker candidate for PD, but a lack of analytical methods to measure complex disease-associated variants of α-syn has prevented its widespread use as a biomarker. Antibody-based methods such as immunoassays and mass spectrometry-based approaches have been used to measure a limited number of α-syn forms; however, these methods fail to differentiate variants of α-syn that display subtle differences in only the sequence and structure. In this work, we developed a cyclic ion mobility-mass spectrometry method that combines multiple stages of activation and timed ion selection to quantify α-syn variants using both mass- and structure-based measurements. This method can allow for the quantification of several α-syn variants present at physiological levels in biological fluid. Taken together, this approach can be used to galvanize future efforts aimed at understanding the underlying mechanisms of PD and serves as a starting point for the development of future protein-structure-based diagnostics and therapeutic interventions.
    MeSH term(s) Humans ; alpha-Synuclein/chemistry ; Parkinson Disease/metabolism ; Neurodegenerative Diseases ; Biomarkers/analysis ; Mass Spectrometry ; Antibodies
    Chemical Substances alpha-Synuclein ; Biomarkers ; Antibodies
    Language English
    Publishing date 2024-04-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.4c00477
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Monitoring hormone and small molecule secretion dynamics from islets-on-chip.

    Lenhart, Ashley E / Kennedy, Robert T

    Analytical and bioanalytical chemistry

    2022  Volume 415, Issue 4, Page(s) 533–544

    Abstract: Tissue functions such as hormone secretion involve the interplay of multiple chemical signals and metabolic processes over time. Measuring the different components involved is useful in unraveling the interactions, but often requires use of multiple ... ...

    Abstract Tissue functions such as hormone secretion involve the interplay of multiple chemical signals and metabolic processes over time. Measuring the different components involved is useful in unraveling the interactions, but often requires use of multiple analytical techniques. The challenge of measuring the necessary components with temporal resolution is greater when tissue samples are limited. Here, an accessible microfluidic platform compatible with multiple measurement techniques to monitor cell secretions has been developed. The platform is applied to islets of Langerhans, micro-organs involved in glucose homeostasis and diabetes. The device houses 1 to 8 islets and the perfusion fluid can be controlled to change conditions, e.g., glucose concentration, in seconds. Samples are collected in fractions and split for offline analysis. The device is paired with a scaled-down immunoassay, AlphaLISA, for hormone quantification and liquid chromatography-mass spectrometry for small molecule quantification to study secretion dynamics. The combined system allows the first simultaneous measurement of insulin, glucagon, biogenic amines, and amino acids from islet secretions. The combined measurements revealed correlation in secretion events and differences in timing of release between hormones and biogenic amines and amino acids. These efforts decreased the number of islets required compared to standard approaches, thus decreasing necessary animal use, reagent use, and cost, while increasing information content achievable from one sample. The microfluidic device is a suitable platform for in-depth characterization of secretion from small tissue samples.
    MeSH term(s) Animals ; Islets of Langerhans/metabolism ; Microfluidic Analytical Techniques ; Insulin/analysis ; Amino Acids/analysis ; Glucose/analysis
    Chemical Substances Insulin ; Amino Acids ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2022-12-02
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 201093-8
    ISSN 1618-2650 ; 0016-1152 ; 0372-7920
    ISSN (online) 1618-2650
    ISSN 0016-1152 ; 0372-7920
    DOI 10.1007/s00216-022-04460-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Brazil at the Center of Chikungunya Outbreaks.

    Amaral, J Kennedy / Taylor, Peter C / Schoen, Robert T

    Journal of global infectious diseases

    2023  Volume 15, Issue 3, Page(s) 131–132

    Language English
    Publishing date 2023-07-12
    Publishing country India
    Document type Journal Article
    ZDB-ID 2545454-7
    ISSN 0974-8245 ; 0974-777X
    ISSN (online) 0974-8245
    ISSN 0974-777X
    DOI 10.4103/jgid.jgid_21_23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Advances in coupling droplet microfluidics to mass spectrometry.

    Murray, Bridget E / Penabad, Laura I / Kennedy, Robert T

    Current opinion in biotechnology

    2023  Volume 82, Page(s) 102962

    Abstract: Droplet microfluidics enables development of workflows with low sample consumption and high throughput. Fluorescence-based assays are most used with droplet microfluidics; however, the requirement of a fluorescent reporter restricts applicability of this ...

    Abstract Droplet microfluidics enables development of workflows with low sample consumption and high throughput. Fluorescence-based assays are most used with droplet microfluidics; however, the requirement of a fluorescent reporter restricts applicability of this approach. The coupling of droplets to mass spectrometry (MS) has enabled selective assays on complex mixtures to broaden the analyte scope. Droplet microfluidics has been interfaced to MS via electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI). The works reviewed herein outline the development of this nascent field as well as initial exploration of its application in biotechnology and bioanalysis, including synthetic biology, reaction development, and in vivo sensing.
    MeSH term(s) Microfluidics/methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Spectrometry, Mass, Electrospray Ionization/methods
    Language English
    Publishing date 2023-06-17
    Publishing country England
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2023.102962
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    Zs.A 3071: Show issues Location:
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