Article ; Online: In-Depth Proteomic Analysis of Blood Circulating Small Extracellular Vesicles.
Methods in molecular biology (Clifton, N.J.)
2023 Volume 2628, Page(s) 279–289
Abstract: Circulating small extracellular vesicles (sEVs), also called exosomes, are key players in the investigation of cell-cell communication mechanisms and in the identification of new potential biomarkers. These particles can carry proteins, DNA, mRNA, miRNA, ...
Abstract | Circulating small extracellular vesicles (sEVs), also called exosomes, are key players in the investigation of cell-cell communication mechanisms and in the identification of new potential biomarkers. These particles can carry proteins, DNA, mRNA, miRNA, lipids and metabolites that are transported all over the human body, potentially reaching all the cells. In particular, proteins, which are well-known biological actors in cell signalling, will be discussed in this context. In this article, we present a mass spectrometry approach for the in-depth characterization of the sEVs proteome. The protocols include strategies for the isolation and purification of sEVs, for the extraction of proteins and the purification of sEVs proteins by the immunodepletion of the most abundant plasmatic proteins. Finally, bioinformatic analysis for the extraction of the most important biological features associated with the proteomic content of sEVs is reported. |
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MeSH term(s) | Humans ; Proteomics/methods ; Extracellular Vesicles/metabolism ; Exosomes/genetics ; Biomarkers/metabolism ; Proteome/metabolism |
Chemical Substances | Biomarkers ; Proteome |
Language | English |
Publishing date | 2023-02-03 |
Publishing country | United States |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ISSN | 1940-6029 |
ISSN (online) | 1940-6029 |
DOI | 10.1007/978-1-0716-2978-9_18 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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