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Article: Autophagy in Metabolic Age-Related Human Diseases.

Moulis, Manon / Vindis, Cecile

2018 Volume 7, Issue 10

Abstract: Autophagy is a highly conserved homeostatic cellular mechanism that mediates the degradation of damaged organelles, protein aggregates, and invading pathogens through a lysosome-dependent pathway. Over the last few years, specific functions of autophagy ... ...

Abstract	Autophagy is a highly conserved homeostatic cellular mechanism that mediates the degradation of damaged organelles, protein aggregates, and invading pathogens through a lysosome-dependent pathway. Over the last few years, specific functions of autophagy have been discovered in many tissues and organs; however, abnormal upregulation or downregulation of autophagy has been depicted as an attribute of a variety of pathologic conditions. In this review, we will describe the current knowledge on the role of autophagy, from its regulation to its physiological influence, in metabolic age-related disorders. Finally, we propose to discuss the therapeutic potential of pharmacological and nutritional modulators of autophagy to treat metabolic diseases.
Language	English
Publishing date	2018-09-24
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2661518-6
ISSN	2073-4409
ISSN	2073-4409
DOI	10.3390/cells7100149
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Autophagy in Metabolic Age-Related Human Diseases

Manon Moulis / Cecile Vindis

Cells, Vol 7, Iss 10, p

2018 Volume 149

Abstract	Autophagy is a highly conserved homeostatic cellular mechanism that mediates the degradation of damaged organelles, protein aggregates, and invading pathogens through a lysosome-dependent pathway. Over the last few years, specific functions of autophagy have been discovered in many tissues and organs; however, abnormal upregulation or downregulation of autophagy has been depicted as an attribute of a variety of pathologic conditions. In this review, we will describe the current knowledge on the role of autophagy, from its regulation to its physiological influence, in metabolic age-related disorders. Finally, we propose to discuss the therapeutic potential of pharmacological and nutritional modulators of autophagy to treat metabolic diseases.
Keywords	autophagy ; metabolism ; diabetes ; obesity ; liver diseases ; atherosclerosis ; pharmacological modulators ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2018-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Methods for Measuring Autophagy in Mice.

Moulis, Manon / Vindis, Cécile

Cells

2017 Volume 6, Issue 2

Abstract: Autophagy is a dynamic intracellular process that mediates the degradation of damaged cytoplasmic components by the lysosome. This process plays important roles in maintaining normal cellular homeostasis and energy balance. Measuring autophagy activity ... ...

Abstract	Autophagy is a dynamic intracellular process that mediates the degradation of damaged cytoplasmic components by the lysosome. This process plays important roles in maintaining normal cellular homeostasis and energy balance. Measuring autophagy activity is critical and although the determination of autophagic flux in isolated cells is well documented, there is a need to have reliable and quantitative assays to evaluate autophagy in whole organisms. Because mouse models have been precious in establishing the functional significance of autophagy under physiological or pathological conditions, we present in this chapter a compendium of the current available methods to measure autophagy in mice, and discuss their advantages and limitations.
Language	English
Publishing date	2017-06-08
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2661518-6
ISSN	2073-4409
ISSN	2073-4409
DOI	10.3390/cells6020014
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Bacterial α-Glucan and Branching Sucrases from GH70 Family: Discovery, Structure-Function Relationship Studies and Engineering.

Molina, Manon / Cioci, Gianluca / Moulis, Claire / Séverac, Etienne / Remaud-Siméon, Magali

Microorganisms

2021 Volume 9, Issue 8

Abstract: Glucansucrases and branching sucrases are classified in the family 70 of glycoside hydrolases. They are produced by lactic acid bacteria occupying very diverse ecological niches (soil, buccal cavity, sourdough, intestine, dairy products, etc.). Usually ... ...

Abstract	Glucansucrases and branching sucrases are classified in the family 70 of glycoside hydrolases. They are produced by lactic acid bacteria occupying very diverse ecological niches (soil, buccal cavity, sourdough, intestine, dairy products, etc.). Usually secreted by their producer organisms, they are involved in the synthesis of α-glucans from sucrose substrate. They contribute to cell protection while promoting adhesion and colonization of different biotopes. Dextran, an α-1,6 linked linear α-glucan, was the first microbial polysaccharide commercialized for medical applications. Advances in the discovery and characterization of these enzymes have remarkably enriched the available diversity with new catalysts. Research into their molecular mechanisms has highlighted important features governing their peculiarities thus opening up many opportunities for engineering these catalysts to provide new routes for the transformation of sucrose into value-added molecules. This article reviews these different aspects with the ambition to show how they constitute the basis for promising future developments.
Language	English
Publishing date	2021-07-28
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms9081607
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Article ; Online: Methods for Measuring Autophagy in Mice

Manon Moulis / Cécile Vindis

Cells, Vol 6, Iss 2, p

2017 Volume 14

Abstract	Autophagy is a dynamic intracellular process that mediates the degradation of damaged cytoplasmic components by the lysosome. This process plays important roles in maintaining normal cellular homeostasis and energy balance. Measuring autophagy activity is critical and although the determination of autophagic flux in isolated cells is well documented, there is a need to have reliable and quantitative assays to evaluate autophagy in whole organisms. Because mouse models have been precious in establishing the functional significance of autophagy under physiological or pathological conditions, we present in this chapter a compendium of the current available methods to measure autophagy in mice, and discuss their advantages and limitations.
Keywords	autophagy ; methods ; flux ; mouse models ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2017-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Bacterial α-Glucan and Branching Sucrases from GH70 Family: Discovery, Structure–Function Relationship Studies and Engineering

Molina, Manon / Cioci, Gianluca / Moulis, Claire / Séverac, Etienne / Remaud-Siméon, Magali

Microorganisms. 2021 July 28, v. 9, no. 8

2021

Abstract	Glucansucrases and branching sucrases are classified in the family 70 of glycoside hydrolases. They are produced by lactic acid bacteria occupying very diverse ecological niches (soil, buccal cavity, sourdough, intestine, dairy products, etc.). Usually secreted by their producer organisms, they are involved in the synthesis of α-glucans from sucrose substrate. They contribute to cell protection while promoting adhesion and colonization of different biotopes. Dextran, an α-1,6 linked linear α-glucan, was the first microbial polysaccharide commercialized for medical applications. Advances in the discovery and characterization of these enzymes have remarkably enriched the available diversity with new catalysts. Research into their molecular mechanisms has highlighted important features governing their peculiarities thus opening up many opportunities for engineering these catalysts to provide new routes for the transformation of sucrose into value-added molecules. This article reviews these different aspects with the ambition to show how they constitute the basis for promising future developments.
Keywords	adhesion ; alternansucrase ; biotopes ; dextran ; glycosidases ; intestines ; lactic acid ; mouth ; soil ; sourdough ; structure-activity relationships ; sucrose ; value added
Language	English
Dates of publication	2021-0728
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms9081607
Database	NAL-Catalogue (AGRICOLA)

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Article: The Multifunctional Sorting Protein PACS-2 Controls Mitophagosome Formation in Human Vascular Smooth Muscle Cells through Mitochondria-ER Contact Sites.

Moulis, Manon / Grousset, Elisa / Faccini, Julien / Richetin, Kevin / Thomas, Gary / Vindis, Cecile

Cells

2019 Volume 8, Issue 6

Abstract: Mitochondria-associated ER membranes (MAMs) are crucial for lipid transport and synthesis, calcium exchange, and mitochondrial functions, and they also act as signaling platforms. These contact sites also play a critical role in the decision between ... ...

Abstract	Mitochondria-associated ER membranes (MAMs) are crucial for lipid transport and synthesis, calcium exchange, and mitochondrial functions, and they also act as signaling platforms. These contact sites also play a critical role in the decision between autophagy and apoptosis with far reaching implications for cell fate. Vascular smooth muscle cell (VSMC) apoptosis accelerates atherogenesis and the progression of advanced lesions, leading to atherosclerotic plaque vulnerability and medial degeneration. Though the successful autophagy of damaged mitochondria promotes VSMC survival against pro-apoptotic atherogenic stressors, it is unknown whether MAMs are involved in VSMC mitophagy processes. Here, we investigated the role of the multifunctional MAM protein phosphofurin acidic cluster sorting protein 2 (PACS-2) in regulating VSMC survival following a challenge by atherogenic lipids. Using high-resolution confocal microscopy and proximity ligation assays, we found an increase in MAM contacts as in PACS-2-associated MAMs upon stimulation with atherogenic lipids. Correspondingly, the disruption of MAM contacts by PACS-2 knockdown impaired mitophagosome formation and mitophagy, thus potentiating VSMC apoptosis. In conclusion, our data shed new light on the significance of the MAM modulatory protein PACS-2 in vascular cell physiopathology and suggest MAMs may be a new target to modulate VSMC fate and favor atherosclerotic plaque stability.
MeSH term(s)	Animals ; Cell Death ; Endoplasmic Reticulum/metabolism ; Humans ; Lipoproteins, LDL ; Mice ; Mitochondria/metabolism ; Mitophagy ; Models, Biological ; Muscle, Smooth, Vascular/cytology ; Myocytes, Smooth Muscle/metabolism ; Phagosomes/metabolism ; Vesicular Transport Proteins/metabolism
Chemical Substances	Lipoproteins, LDL ; PACS2 protein, human ; Vesicular Transport Proteins ; oxidized low density lipoprotein
Language	English
Publishing date	2019-06-25
Publishing country	Switzerland
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2661518-6
ISSN	2073-4409
ISSN	2073-4409
DOI	10.3390/cells8060638
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Identification and implication of tissue-enriched ligands in epithelial-endothelial crosstalk during pancreas development.

Moulis, Manon / Runser, Steve Vincent Maurice / Glorieux, Laura / Dauguet, Nicolas / Vanderaa, Christophe / Gatto, Laurent / Tyteca, Donatienne / Henriet, Patrick / Spagnoli, Francesca M / Iber, Dagmar / Pierreux, Christophe E

Scientific reports

2022 Volume 12, Issue 1, Page(s) 12498

Abstract: Development of the pancreas is driven by an intrinsic program coordinated with signals from other cell types in the epithelial environment. These intercellular communications have been so far challenging to study because of the low concentration, ... ...

Abstract	Development of the pancreas is driven by an intrinsic program coordinated with signals from other cell types in the epithelial environment. These intercellular communications have been so far challenging to study because of the low concentration, localized production and diversity of the signals released. Here, we combined scRNAseq data with a computational interactomic approach to identify signals involved in the reciprocal interactions between the various cell types of the developing pancreas. This in silico approach yielded 40,607 potential ligand-target interactions between the different main pancreatic cell types. Among this vast network of interactions, we focused on three ligands potentially involved in communications between epithelial and endothelial cells. BMP7 and WNT7B, expressed by pancreatic epithelial cells and predicted to target endothelial cells, and SEMA6D, involved in the reverse interaction. In situ hybridization confirmed the localized expression of Bmp7 in the pancreatic epithelial tip cells and of Wnt7b in the trunk cells. On the contrary, Sema6d was enriched in endothelial cells. Functional experiments on ex vivo cultured pancreatic explants indicated that tip cell-produced BMP7 limited development of endothelial cells. This work identified ligands with a restricted tissular and cellular distribution and highlighted the role of BMP7 in the intercellular communications contributing to vessel development and organization during pancreas organogenesis.
MeSH term(s)	Cell Differentiation/physiology ; Endothelial Cells/metabolism ; Ligands ; Organogenesis/physiology ; Pancreas/metabolism
Chemical Substances	Ligands
Language	English
Publishing date	2022-07-21
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-16072-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A specific oligosaccharide-binding site in the alternansucrase catalytic domain mediates alternan elongation.

Molina, Manon / Moulis, Claire / Monties, Nelly / Guieysse, David / Morel, Sandrine / Cioci, Gianluca / Remaud-Siméon, Magali

The Journal of biological chemistry

2020 Volume 295, Issue 28, Page(s) 9474–9489

Abstract: Microbial α-glucans produced by GH70 (glycoside hydrolase family 70) glucansucrases are gaining importance because of the mild conditions for their synthesis from sucrose, their biodegradability, and their current and anticipated applications that ... ...

Abstract	Microbial α-glucans produced by GH70 (glycoside hydrolase family 70) glucansucrases are gaining importance because of the mild conditions for their synthesis from sucrose, their biodegradability, and their current and anticipated applications that largely depend on their molar mass. Focusing on the alternansucrase (ASR) from
MeSH term(s)	Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Binding Sites ; Crystallography, X-Ray ; Glucans/biosynthesis ; Glucans/chemistry ; Glycosyltransferases/chemistry ; Glycosyltransferases/genetics ; Glycosyltransferases/metabolism ; Leuconostoc/enzymology ; Protein Domains
Chemical Substances	Bacterial Proteins ; Glucans ; alternan ; Glycosyltransferases (EC 2.4.-) ; alternansucrase (EC 2.4.1.140)
Language	English
Publishing date	2020-05-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1074/jbc.RA120.013028
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: The Multifunctional Sorting Protein PACS-2 Controls Mitophagosome Formation in Human Vascular Smooth Muscle Cells through Mitochondria-ER Contact Sites

Manon Moulis / Elisa Grousset / Julien Faccini / Kevin Richetin / Gary Thomas / Cecile Vindis

Cells, Vol 8, Iss 6, p

2019 Volume 638

Abstract	Mitochondria-associated ER membranes (MAMs) are crucial for lipid transport and synthesis, calcium exchange, and mitochondrial functions, and they also act as signaling platforms. These contact sites also play a critical role in the decision between autophagy and apoptosis with far reaching implications for cell fate. Vascular smooth muscle cell (VSMC) apoptosis accelerates atherogenesis and the progression of advanced lesions, leading to atherosclerotic plaque vulnerability and medial degeneration. Though the successful autophagy of damaged mitochondria promotes VSMC survival against pro-apoptotic atherogenic stressors, it is unknown whether MAMs are involved in VSMC mitophagy processes. Here, we investigated the role of the multifunctional MAM protein phosphofurin acidic cluster sorting protein 2 (PACS-2) in regulating VSMC survival following a challenge by atherogenic lipids. Using high-resolution confocal microscopy and proximity ligation assays, we found an increase in MAM contacts as in PACS-2-associated MAMs upon stimulation with atherogenic lipids. Correspondingly, the disruption of MAM contacts by PACS-2 knockdown impaired mitophagosome formation and mitophagy, thus potentiating VSMC apoptosis. In conclusion, our data shed new light on the significance of the MAM modulatory protein PACS-2 in vascular cell physiopathology and suggest MAMs may be a new target to modulate VSMC fate and favor atherosclerotic plaque stability.
Keywords	PACS-2 ; mitochondria-associated ER membranes (MAMs) ; mitochondria ; mitophagy ; apoptosis ; atherosclerosis ; vascular smooth muscle cell ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2019-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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