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Article ; Online: Formulating of the sustained release of Tebuconazole pesticide using chitosan aerogel reinforced NFC/CaCO

Montaser, Ahmed S / Abdelhameed, Reda M / Shaheen, Tharwat I

International journal of biological macromolecules

2023 Volume 256, Issue Pt 1, Page(s) 128419

Abstract: Chitosan-based aerogels were fabricated through utilizing of nanofibrillated cellulose (NFC)/ ... ...

Abstract	Chitosan-based aerogels were fabricated through utilizing of nanofibrillated cellulose (NFC)/CaCO
MeSH term(s)	Chitosan/chemistry ; Cellulose/chemistry ; Calcium ; Pesticides ; Delayed-Action Preparations ; Nanocomposites ; Triazoles
Chemical Substances	Chitosan (9012-76-4) ; Cellulose (9004-34-6) ; tebuconazole (401ATW8TRW) ; Calcium (SY7Q814VUP) ; Pesticides ; Delayed-Action Preparations ; Triazoles
Language	English
Publishing date	2023-11-25
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2023.128419
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Article ; Online: In vivo assessment of the durable, green and in situ bio-functional cotton fabrics based carboxymethyl chitosan nanohybrid for wound healing application.

Shaheen, Tharwat I / Abdelhameed, Mohamed F / Zaghloul, Saad / Montaser, A S

International journal of biological macromolecules

2022 Volume 209, Issue Pt A, Page(s) 485–497

Abstract: Herein, a newly developed approach for durable antibacterial cotton fabrics coated carboxymethyl chitosan (CMCs) via ionic crosslinking driven by cationization of cotton surface (CC) with 3-chloro-2-hydroxyl propyl-trimethyl ammonium chloride (CHTAC) was ...

Abstract	Herein, a newly developed approach for durable antibacterial cotton fabrics coated carboxymethyl chitosan (CMCs) via ionic crosslinking driven by cationization of cotton surface (CC) with 3-chloro-2-hydroxyl propyl-trimethyl ammonium chloride (CHTAC) was achieved. In this regard, the novelty was extended to impart a highly antibacterial activity through harnessing of the as-functionalized CMCs/CC for in situ preparation of AgNPs, without using of hazardous reductants. The antibacterial activity of the in situ prepared AgNPs onto CMCs/CC as well as the in vivo study on the rat lab were investigated to evaluate their healing efficiency, pathological tissues and biomarkers. Results affirmed that the treatment of CC with 10% of CMCs was adequate to achieve the highest swelling ratio which, in turns, is able to in situ deposition of AgNPs with a size range of 2-10 nm onto CC/CMCs rendering them a highly durable antibacterial activity against both Gram +Ve and Gram -Ve bacteria, which had a bacterial reduction of 98% to 86% after 20 washing cycles. Furthermore, the in vivo study revealed effectively the advantageous uses of the cotton functionalized with AgNPs compared to CC/CMCs in wound healing via alleviating the oxidative stress and promoting hyaluronic acid in wounded skin as well as increasing RUNX2 in healed skin tissues.
MeSH term(s)	Animals ; Anti-Bacterial Agents/pharmacology ; Chitosan/pharmacology ; Metal Nanoparticles ; Rats ; Silver/pharmacology ; Textiles ; Wound Healing
Chemical Substances	Anti-Bacterial Agents ; Silver (3M4G523W1G) ; Chitosan (9012-76-4)
Language	English
Publishing date	2022-04-08
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2022.04.027
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Article ; Online: Effect of cellulose nanocrystals on scaffolds comprising chitosan, alginate and hydroxyapatite for bone tissue engineering.

Shaheen, Th I / Montaser, A S / Li, Suming

International journal of biological macromolecules

2018 Volume 121, Page(s) 814–821

Abstract: In this study, chitosan/alginate/hydroxyapatite/nanocrystalline cellulose scaffolds were successfully fabricated by the using of freeze-drying method, followed by dicationic crosslinking using ... ...

Abstract	In this study, chitosan/alginate/hydroxyapatite/nanocrystalline cellulose scaffolds were successfully fabricated by the using of freeze-drying method, followed by dicationic crosslinking using CaCl
MeSH term(s)	Biocompatible Materials/chemistry ; Biocompatible Materials/pharmacology ; Bone and Bones/cytology ; Calcium Chloride/chemistry ; Cell Differentiation/drug effects ; Cell Line ; Cell Proliferation/drug effects ; Cellulose/chemistry ; Chitosan/chemistry ; Durapatite/chemistry ; Humans ; Nanoparticles/chemistry ; Osteoblasts/cytology ; Osteoblasts/drug effects ; Tissue Engineering ; Tissue Scaffolds/chemistry
Chemical Substances	Biocompatible Materials ; Cellulose (9004-34-6) ; Chitosan (9012-76-4) ; Durapatite (91D9GV0Z28) ; Calcium Chloride (M4I0D6VV5M)
Language	English
Publishing date	2018-10-17
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2018.10.081
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Article ; Online: In vivo assessment of the durable, green and in situ bio-functional cotton fabrics based carboxymethyl chitosan nanohybrid for wound healing application

Shaheen, Tharwat I. / Abdelhameed, Mohamed F. / Zaghloul, Saad / Montaser, A.S.

International Journal of Biological Macromolecules. 2022 June, v. 209 p.485-497

2022

Abstract	Herein, a newly developed approach for durable antibacterial cotton fabrics coated carboxymethyl chitosan (CMCs) via ionic crosslinking driven by cationization of cotton surface (CC) with 3-chloro-2-hydroxyl propyl-trimethyl ammonium chloride (CHTAC) was achieved. In this regard, the novelty was extended to impart a highly antibacterial activity through harnessing of the as-functionalized CMCs/CC for in situ preparation of AgNPs, without using of hazardous reductants. The antibacterial activity of the in situ prepared AgNPs onto CMCs/CC as well as the in vivo study on the rat lab were investigated to evaluate their healing efficiency, pathological tissues and biomarkers. Results affirmed that the treatment of CC with 10% of CMCs was adequate to achieve the highest swelling ratio which, in turns, is able to in situ deposition of AgNPs with a size range of 2-10 nm onto CC/CMCs rendering them a highly durable antibacterial activity against both Gram +Ve and Gram -Ve bacteria, which had a bacterial reduction of 98% to 86% after 20 washing cycles. Furthermore, the in vivo study revealed effectively the advantageous uses of the cotton functionalized with AgNPs compared to CC/CMCs in wound healing via alleviating the oxidative stress and promoting hyaluronic acid in wounded skin as well as increasing RUNX2 in healed skin tissues.
Keywords	ammonium chloride ; antibacterial properties ; biomarkers ; cationization ; chitosan ; cotton ; crosslinking ; hyaluronic acid ; nanohybrids ; oxidative stress ; rats ; reducing agents ; Carboxymethyl chitosan ; Silver nanoparticles ; Wound healing
Language	English
Dates of publication	2022-06
Size	p. 485-497.
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2022.04.027
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Article: Effect of cellulose nanocrystals on scaffolds comprising chitosan, alginate and hydroxyapatite for bone tissue engineering

Shaheen, Th.I / A.S. Montaser / Suming Li

International journal of biological macromolecules. 2019 Jan., v. 121

2019

Abstract	In this study, chitosan/alginate/hydroxyapatite/nanocrystalline cellulose scaffolds were successfully fabricated by the using of freeze-drying method, followed by dicationic crosslinking using CaCl2. The chemical structure and morphology along with mechanical properties of the formed scaffolds respecting to various CNC contents were studied by Fourier-transform infrared spectroscopy (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and mechanical compression test. Chemical interaction and electrostatic attraction between chitosan (CS) and alginate with various CNC ratios were affirmed by FTIR spectroscopy. Results depicted that, scaffolds containing CNC exhibited remarkable improvement in both swelling ratio up to 110% compared without CNC (63%) and compressive strength when compared with other scaffolds. In addition, the average pore size increased, dramatically, with increasing of CNC up to 230 μm. Porosity was also obeyed the sequence and attainted a maximum value at 93.6%. The growth and cell attachment of fibroblast cells of the selected scaffold were examined prolonging to the cell viability by using Alamar Blue (AB) and then confirmed using SEM. The results indicated that the scaffold comprising CNC has a promising cell growth and cell adherence, and thus expected to have a potent possibility for applications in bone tissue culture.
Keywords	Fourier transform infrared spectroscopy ; X-ray diffraction ; alginates ; bones ; calcium chloride ; cell growth ; cell viability ; cellulose ; chitosan ; compression strength ; crosslinking ; electrostatic interactions ; fibroblasts ; freeze drying ; hydroxyapatite ; mechanical properties ; nanocrystals ; porosity ; scanning electron microscopy ; tissue culture ; tissue engineering
Language	English
Dates of publication	2019-01
Size	p. 814-821.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2018.10.081
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Article ; Online: Non-surgical periodontal therapy with adjunct photodynamic therapy for the management of periodontal inflammation in adults using nicotine-free electronic-cigarette: A randomized control trial.

Alshibani, Nouf / Alssum, Lamees / Basudan, Amani / Shaheen, Marwa / Alqutub, Montaser N / Dahash, Fahda Al / Alkattan, Reem

Photodiagnosis and photodynamic therapy

2022 Volume 38, Page(s) 102820

Abstract: Objective: The aim was to assess the effect of non-surgical periodontal therapy (NSPT) with adjunct photodynamic treatment (PDT) for the management of periodontal inflammation in young electronic cigarette (E-cig) users.: Methods: Patients with ... ...

Abstract	Objective: The aim was to assess the effect of non-surgical periodontal therapy (NSPT) with adjunct photodynamic treatment (PDT) for the management of periodontal inflammation in young electronic cigarette (E-cig) users. Methods: Patients with periodontal inflammation were included. Patient demographics and information related to E-Cig usage were recorded. Scores of plaque index (PI), bleeding index (BI), clinical attachment loss (AL) and probing depth (PD) recorded at baseline and at 3-months' follow-up. Patients were randomly divided into test (NSPT + PDT) and control groups (NSPT) alone. Sample-size estimation was done using data from a pilot investigation and group comparisons were done. Correlation between periodontal parameters and duration of E-cig use was assessed using regression analysis models. Group-comparisons were done using the Mann Whitney U test; and logistic regression was done to correlate periodontal parameters with age, gender, frequency of vaping, number of puffs inhaled and oral hygiene maintenance protocols. Level of significance was set at P<0.01. Results: Twenty-three and 23 individuals were randomly allocated to the test- and control-group, respectively. At baseline, PI, BI and PD were comparable in all patients. There was a significant reduction in PI (P<0.01), BI (P<0.01) and PD (P<0.01) in the test and control groups at 3-months' follow-up when compared with their respective baseline scores. At 3-months' follow-up, there was no significant difference in PI, BI and PD among patients in the test and control groups. There was no clinical evidence of clinical AL among patients in the test- and control groups at baseline and at 3-months' follow-up. Conclusion: In the short-term, PDT is as effective as NSPT for the management of periodontal inflammation in young E-Cig users.
MeSH term(s)	Adult ; Electronic Nicotine Delivery Systems ; Electronics ; Humans ; Inflammation ; Nicotine/adverse effects ; Photochemotherapy/methods ; Tobacco Products
Chemical Substances	Nicotine (6M3C89ZY6R)
Language	English
Publishing date	2022-03-21
Publishing country	Netherlands
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	2149918-4
ISSN	1873-1597 ; 1572-1000
ISSN (online)	1873-1597
ISSN	1572-1000
DOI	10.1016/j.pdpdt.2022.102820
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Article ; Online: Postoperative Analgesic and Anti-inflammatory Effectiveness of Ginger (Zingiber officinale) and NSAIDs as Adjuncts to Nonsurgical Periodontal Therapy for the Management of Periodontitis.

Alshibani, Nouf / Al-Kattan, Reem / Alssum, Lamees / Basudan, Amani / Shaheen, Marwa / Alqutub, Montaser N / Al Dahash, Fahda

Oral health & preventive dentistry

2022 Volume 20, Issue 1, Page(s) 227–232

Abstract: Purpose: The authors hypothesize that ginger (Zingiber officinale) tablets and non-steroidal anti-inflammatory drugs (NSAIDs) are effective in reducing postoperative self-rated pain and periodontal parameters (plaque index [PI], gingival index [GI], and ...

Abstract	Purpose: The authors hypothesize that ginger (Zingiber officinale) tablets and non-steroidal anti-inflammatory drugs (NSAIDs) are effective in reducing postoperative self-rated pain and periodontal parameters (plaque index [PI], gingival index [GI], and probing depth [PD], clinical attachment loss [AL] and marginal bone loss) following non-surgical periodontal therapy (NSPT) in patients with periodontitis. The aim was to compare the postoperative analgesic and anti-inflammatory effectiveness of ginger tablets and NSAIDs as adjuncts to nonsurgical periodontal therapy for the management of periodontitis. Materials and Methods: Patients with periodontitis were included. All patients underwent NSPT. In groups 1 and 2, patients received postoperative ginger (400 mg) and non-steroidal anti-inflammatory drugs (400 mg), respectively. Demographic data were collected, and full-mouth periodontal parameters (PI, GI, PD and CAL) were evaluated at baseline and at 7, 14 and 21 days. Self-rated pain scores were assessed at baseline, and at 24 h, 3 and 7 days of follow-up. In both groups, self-rated pain was assessed pre- and postoperatively using the numeric rating scale (NRS). Power analysis was performed on data from a pilot investigation and group comparisons were done. Statistical significance was set at p < 0.01. Results: Baseline mean NRS scores in groups 1 and 2 were 4.19 ± 0.12 and 4.13 ± 0.08, respectively. All participants had stage II/grade B periodontitis. At baseline, self-rated pain scores were significantly higher among patients in groups 1 and 2 at 24 h (p < 0.01) and 3 days (p < 0.01) of follow-up. In groups 1 (p < 0.01) and 2 (p < 0.01), self-rated pain scores were significantly higher at 24 h compared with 3 days of follow-up. In both groups, there was a significant reduction in PI (p < 0.01), GI (p < 0.01) and PD (p < 0.01) at 7, 14 and 21 days of follow-up compared with baseline. Conclusion: Ginger and traditional NSAIDs are effective in reducing postoperative pain and inflammation following NSPT in patients with moderate periodontitis.
MeSH term(s)	Anti-Inflammatory Agents/therapeutic use ; Anti-Inflammatory Agents, Non-Steroidal/therapeutic use ; Chronic Periodontitis/drug therapy ; Dental Scaling ; Follow-Up Studies ; Zingiber officinale ; Humans ; Pain/drug therapy ; Periodontal Attachment Loss ; Periodontitis/drug therapy
Chemical Substances	Anti-Inflammatory Agents ; Anti-Inflammatory Agents, Non-Steroidal
Language	English
Publishing date	2022-07-05
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2136786-3
ISSN	1757-9996 ; 1602-1622
ISSN (online)	1757-9996
ISSN	1602-1622
DOI	10.3290/j.ohpd.b3125633
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Article ; Online: A Phase II Trial of the CD40 Agonistic Antibody Sotigalimab (APX005M) in Combination with Nivolumab in Subjects with Metastatic Melanoma with Confirmed Disease Progression on Anti-PD-1 Therapy.

Weiss, Sarah A / Sznol, Mario / Shaheen, Montaser / Berciano-Guerrero, Miguel-Ángel / Couselo, Eva Muñoz / Rodríguez-Abreu, Delvys / Boni, Valentina / Schuchter, Lynn M / Gonzalez-Cao, Maria / Arance, Ana / Wei, Wei / Ganti, Apar Kishor / Hauke, Ralph J / Berrocal, Alfonso / Iannotti, Nicholas O / Hsu, Frank J / Kluger, Harriet M

Clinical cancer research : an official journal of the American Association for Cancer Research

2023 Volume 30, Issue 1, Page(s) 74–81

Abstract: Purpose: Disease progression during or after anti-PD-1-based treatment is common in advanced melanoma. Sotigalimab is a CD40 agonist antibody with a unique epitope specificity and Fc receptor binding profile optimized for activation of CD40-expressing ... ...

Abstract	Purpose: Disease progression during or after anti-PD-1-based treatment is common in advanced melanoma. Sotigalimab is a CD40 agonist antibody with a unique epitope specificity and Fc receptor binding profile optimized for activation of CD40-expressing antigen-presenting cells. Preclinical data indicated that CD40 agonists combined with anti-PD1 could overcome resistance to anti-PD-1. Patients and methods: We conducted a multicenter, open-label, phase II trial to evaluate the combination of sotigalimab 0.3 mg/kg and nivolumab 360 mg every 3 weeks in patients with advanced melanoma following confirmed disease progression on a PD-1 inhibitor. The primary objective was to determine the objective response rate (ORR). Results: Thirty-eight subjects were enrolled and evaluable for safety. Thirty-three were evaluable for activity. Five confirmed partial responses (PR) were observed for an ORR of 15%. Two PRs are ongoing at 45.9+ and 26+ months, whereas the other three responders relapsed at 41.1, 18.7, and 18.4 months. The median duration of response was at least 26 months. Two additional patients had stable disease for >6 months. Thirty-four patients (89%) experienced at least one adverse event (AE), and 13% experienced a grade 3 AE related to sotigalimab. The most common AEs were pyrexia, chills, nausea, fatigue, pruritus, elevated liver function, rash, vomiting, headache, arthralgia, asthenia, myalgia, and diarrhea. There were no treatment-related SAEs, deaths, or discontinuation of sotigalimab due to AEs. Conclusions: Sotigalimab plus nivolumab had a favorable safety profile consistent with the toxicity profiles of each agent. The combination resulted in durable and prolonged responses in a subset of patients with anti-PD-1-resistant melanoma, warranting further evaluation in this setting. See related commentary by Wu and Luke, p. 9.
MeSH term(s)	Humans ; Nivolumab/adverse effects ; Melanoma/pathology ; Antibodies, Monoclonal/adverse effects ; Disease Progression ; Antineoplastic Combined Chemotherapy Protocols/adverse effects
Chemical Substances	Nivolumab (31YO63LBSN) ; sotigalimab ; Antibodies, Monoclonal
Language	English
Publishing date	2023-06-27
Publishing country	United States
Document type	Clinical Trial, Phase II ; Multicenter Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1225457-5
ISSN	1557-3265 ; 1078-0432
ISSN (online)	1557-3265
ISSN	1078-0432
DOI	10.1158/1078-0432.CCR-23-0475
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Article ; Online: Tumor microenvironment changes leading to resistance of immune checkpoint inhibitors in metastatic melanoma and strategies to overcome resistance.

Pulluri, Bhargavi / Kumar, Abhijeet / Shaheen, Montaser / Jeter, Joanne / Sundararajan, Srinath

Pharmacological research

2017 Volume 123, Page(s) 95–102

Abstract: Immunotherapy with checkpoint inhibitors targeting CTLA-4 and/or PD-1 receptors independent of the BRAF mutational status and targeted therapy with BRAF and MEK inhibitors in BRAF V600 mutated patients have taken the forefront of advanced melanoma ... ...

Abstract	Immunotherapy with checkpoint inhibitors targeting CTLA-4 and/or PD-1 receptors independent of the BRAF mutational status and targeted therapy with BRAF and MEK inhibitors in BRAF V600 mutated patients have taken the forefront of advanced melanoma treatment. The main advantage of immunotherapy is its ability to provide durable responses in a subset of patients. However, significant proportions of patients either do not respond or have progression after initial response to immunotherapies. Multiple changes in the tumor microenvironment, such as down regulation of immune checkpoint ligands by tumor, alteration in interferon signaling, and activation of alternate immune suppressive pathways, have been identified as possible reasons for failure of immune checkpoint therapy. Here, we review the resistance mechanisms adopted by cancer cells to checkpoint inhibitor therapy and targeted therapy. In addition, we focus on the available and emerging evidence on tumor microenvironment modulation by BRAF/MEK inhibitor therapy and its role in improving responses to checkpoint inhibitor therapy.
Language	English
Publishing date	2017-09
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	1003347-6
ISSN	1096-1186 ; 0031-6989 ; 1043-6618
ISSN (online)	1096-1186
ISSN	0031-6989 ; 1043-6618
DOI	10.1016/j.phrs.2017.07.006
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Article ; Online: TATDN2 resolution of R-loops is required for survival of BRCA1-mutant cancer cells.

Jaiswal, Aruna S / Dutta, Arijit / Srinivasan, Gayathri / Yuan, Yaxia / Zhou, Daohong / Shaheen, Montaser / Sadideen, Doraid T / Kirby, Austin / Williamson, Elizabeth A / Gupta, Yogesh K / Olsen, Shaun K / Xu, Mingjiang / Loranc, Eva / Mukhopadhyay, Pramiti / Pertsemlidis, Alexander / Bishop, Alexander J R / Sung, Patrick / Nickoloff, Jac A / Hromas, Robert

Nucleic acids research

2023 Volume 51, Issue 22, Page(s) 12224–12241

Abstract: BRCA1-deficient cells have increased IRE1 RNase, which degrades multiple microRNAs. Reconstituting expression of one of these, miR-4638-5p, resulted in synthetic lethality in BRCA1-deficient cancer cells. We found that miR-4638-5p represses expression of ...

Abstract	BRCA1-deficient cells have increased IRE1 RNase, which degrades multiple microRNAs. Reconstituting expression of one of these, miR-4638-5p, resulted in synthetic lethality in BRCA1-deficient cancer cells. We found that miR-4638-5p represses expression of TATDN2, a poorly characterized member of the TATD nuclease family. We discovered that human TATDN2 has RNA 3' exonuclease and endonuclease activity on double-stranded hairpin RNA structures. Given the cleavage of hairpin RNA by TATDN2, and that BRCA1-deficient cells have difficulty resolving R-loops, we tested whether TATDN2 could resolve R-loops. Using in vitro biochemical reconstitution assays, we found TATDN2 bound to R-loops and degraded the RNA strand but not DNA of multiple forms of R-loops in vitro in a Mg2+-dependent manner. Mutations in amino acids E593 and E705 predicted by Alphafold-2 to chelate an essential Mg2+ cation completely abrogated this R-loop resolution activity. Depleting TATDN2 increased cellular R-loops, DNA damage and chromosomal instability. Loss of TATDN2 resulted in poor replication fork progression in the presence of increased R-loops. Significantly, we found that TATDN2 is essential for survival of BRCA1-deficient cancer cells, but much less so for cognate BRCA1-repleted cancer cells. Thus, we propose that TATDN2 is a novel target for therapy of BRCA1-deficient cancers.
MeSH term(s)	Humans ; BRCA1 Protein/genetics ; BRCA1 Protein/metabolism ; DNA Replication ; Genomic Instability ; Magnesium ; MicroRNAs/genetics ; Neoplasms/genetics ; R-Loop Structures
Chemical Substances	BRCA1 Protein ; BRCA1 protein, human ; Magnesium (I38ZP9992A) ; MicroRNAs ; long non-coding RNA TATDN1, human
Language	English
Publishing date	2023-11-13
Publishing country	England
Document type	Journal Article
ZDB-ID	186809-3
ISSN	1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
ISSN (online)	1362-4962 ; 1362-4954
ISSN	0301-5610 ; 0305-1048
DOI	10.1093/nar/gkad952
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