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  1. Article ; Online: Algunos parámetros farmacognósticos de Vernonanthura patens (Kunth) H. Rob. (Asteraceae) endémica de Ecuador Some pharmacognostic parameters of native Vernonanthura patens (Kunth) H. Rob. (Asteraceae) from Ecuador

    Patricia Manzano Santana / Tulio Orellana León / Migdalia Miranda Martínez / Juan Abreu Payrol / Omar Ruíz / Esther L Peralta García

    Revista Cubana de Plantas Medicinales, Vol 18, Iss 1, Pp 131-

    2013  Volume 139

    Abstract: Introducción: Vernonanthura patens (Kunth) H. Rob., arbusto originario de Sudamérica, crece ... que las hojas y tallos. Introduction: Vernonanthura patens (Kunth) H. Rob., a native shrub from South America ...

    Abstract Introducción: Vernonanthura patens (Kunth) H. Rob., arbusto originario de Sudamérica, crece silvestre en el sur ecuatoriano; los pobladores del cantón Marcabelí, provincia del Oro, la conocen como laritaco y utilizan las cocciones acuosas de las hojas para lavar y cicatrizar las heridas, calmar el dolor de cabeza, como antiinflamatorio, calmar la tos y combatir ciertos tipos de cáncer. Las investigaciones biológicas preliminares realizadas han demostrado una buena actividad antimalárica y antileishmaniasis. No existen, sin embargo, antecedentes de otras investigaciones farmacológicas ni químicas para la especie. Objetivo: determinar los parámetros físico-químicos de calidad de las flores, hojas y ramas de la especie en estado de fructificación, y los metabolitos secundarios presentes en estos órganos vegetales a través del tamizaje fitoquímico. Métodos: la planta se recolectó en estado adulto, en época de floración, los órganos vegetales secados en estufa se molinaron hasta tamaño de partícula 2 mm. Los índices numéricos se determinaron según la norma ramal de salud pública 309 de 1992, y el tamizaje fitoquímico se realizó según metodología analítica de Miranda y Cuéllar. Se utilizó estadística descriptiva básica para determinar el comportamiento estadístico de los datos (medidas de tendencia central y dispersión). Para obtener diferencias estadísticas significativas, se utilizó el análisis de varianza y la prueba a posteriori de Tukey. Se usó el análisis multivariado de componentes principales para determinar, a través de un biplot, las relaciones multivariadas entre los parámetros de estudio. Para este análisis se usó la versión 2010 de InfoStat Softwware, del Grupo InfoStat FCA, de la Universidad Nacional de Córdoba, Argentina. Resultados: se encontraron diferencias significativas para algunos índices numéricos y en los resultados del tamizaje fitoquímico entre los diferentes órganos vegetativos. Conclusiones: las flores en términos generales presentan mayor porcentaje de sustancias solubles en agua y alcohol, menor humedad residual y mayor abundancia en metabolitos secundarios que las hojas y tallos. Introduction: Vernonanthura patens (Kunth) H. Rob., a native shrub from South America, grows wild in southern Ecuador. The people of Canton Marcabelí, El Oro province, know it as "laritaco" and use aqueous brews of the leaves to wash and heal wounds, to soothe headaches, to reduce inflammation, to soothe coughs and to combat certain types of cancer. Preliminary biological research studies have shown good antimalarial and antileishmanial activity. However, there is no history of other pharmacological or chemical studies about the species. Objective: to determine the quality parameters of the flowers, leaves and branches of the species in the fructification stage as well as to determine the secondary metabolites present through the phytochemical screening. Methods: the plant was harvested in mature state, at time of flowering; the parts of the plant were dried in an oven and then milled to 2 mm particle size. The indexes were determined according to NRSP 309, 1992 and phytochemical screening was performed according to Miranda and Cuellar's analytical methodology 2000. Basic summary statistics was used to determine the statistical data behavior (measures of central tendency and dispersion). To obtain significant statistical differences, the variance analysis and Tukey post-test were used. The multivariate analysis of main components served to determine, through a biplot, the multivariate relationships among the study parameters. For this analysis, the 2010 InfoStat Software version of Group InfoStat FCA, National University of Cordoba, Argentina was the choice. Results: significant differences were found for some numeric indexes and in the results of phytochemical screening among the different vegetative organs. Conclusions: the flowers generally have a higher percentage of water -and alcohol- soluble substances, less residual moisture and are richer in secondary metabolites than the leaves and the stalks.
    Keywords Vernonanthura patens ; laritaco ; parámetros farmacognósticos ; Vernonanthura pathens ; pharmacognitive parameters ; Medicine (General) ; R5-920 ; Medicine ; R ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
    Language Spanish
    Publishing date 2013-03-01T00:00:00Z
    Publisher ECIMED
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Estudio químico de los compuestos lipídicos de las hojas, tallos y flores de Vernonanthura patens (Kunth) H. Rob. (Asteraceae)

    Patricia Manzano Santana / Migdalia Miranda Martínez / Rodney Montes de Oca Porto / Tulio Orellana León / Juan Abreu Payrol / Esther L Peralta García

    Revista Cubana de Plantas Medicinales, Vol 18, Iss 4, Pp 575-

    2013  Volume 585

    Abstract: Introducción: Vernonanthura patens (Kunth) H. Rob. es una especie que crece silvestre en el Ecuador ...

    Abstract Introducción: Vernonanthura patens (Kunth) H. Rob. es una especie que crece silvestre en el Ecuador, para la cual existen escasos antecedentes de estudios químicos y biológicos, y en ninguno de estos se aborda el estudio de fracciones lipídicas. Objetivos: estudiar las fracciones de compuestos lipídicos presentes en las hojas, tallos y flores de la especie. Métodos: la planta se recolectó en estado adulto, en época de floración, los órganos vegetales fueron secados en estufa por separado, se molinaron hasta tamaño de partícula 2 mm y se extrajeron por maceración con etanol. Los extractos etanólicos concentrados se separaron por columna cromatográfica, las fracciones lipídicas separadas con hexano fueron saponificadas y analizadas por el sistema acoplado de cromatografía gaseosa-espectrometría de masas. Resultados: se identificaron los ácidos grasos y compuestos insaponificables presentes en los órganos estudiados, encontrándose algunas diferencias de composición entre estos. Conclusiones: se identificaron 29 ácidos grasos y 8 compuestos insaponificables presentes en los órganos vegetales estudiados.
    Keywords Vernonanthura patens ; ácidos grasos ; compuestos insaponificables ; estudio químico ; Medicine (General) ; R5-920 ; Medicine ; R
    Language Spanish
    Publishing date 2013-12-01T00:00:00Z
    Publisher ECIMED
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: S6K1ing to ResTOR Adipogenesis with Polycomb.

    Juan, Aster H / Sartorelli, Vittorio

    Molecular cell

    2016  Volume 64, Issue 4, Page(s) 850

    Language English
    Publishing date 2016--17
    Publishing country United States
    Document type Journal Article ; Published Erratum
    ZDB-ID 1415236-8
    ISSN 1097-4164 ; 1097-2765
    ISSN (online) 1097-4164
    ISSN 1097-2765
    DOI 10.1016/j.molcel.2016.10.031
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: S6K1ing to ResTOR Adipogenesis with Polycomb.

    Juan, Aster H / Sartorelli, Vittorio

    Molecular cell

    2016  Volume 62, Issue 3, Page(s) 325–326

    Abstract: Signal-directed chromatin recruitment of mammalian Polycomb complexes is a fundamental component of epigenetic regulation. In this issue, Yi et al. (2016) reveal how mTORC1 activation deploys the ribosomal serine/threonine kinase S6K1 and Polycomb ... ...

    Abstract Signal-directed chromatin recruitment of mammalian Polycomb complexes is a fundamental component of epigenetic regulation. In this issue, Yi et al. (2016) reveal how mTORC1 activation deploys the ribosomal serine/threonine kinase S6K1 and Polycomb proteins at genomic regulatory regions to repress expression of anti-adipogenic developmental regulators.
    MeSH term(s) Adipogenesis ; Animals ; Chromatin ; Drosophila Proteins/genetics ; Epigenesis, Genetic ; Polycomb-Group Proteins/genetics ; TOR Serine-Threonine Kinases/genetics
    Chemical Substances Chromatin ; Drosophila Proteins ; Polycomb-Group Proteins ; TOR Serine-Threonine Kinases (EC 2.7.1.1)
    Language English
    Publishing date 2016--05
    Publishing country United States
    Document type Journal Article ; Comment
    ZDB-ID 1415236-8
    ISSN 1097-4164 ; 1097-2765
    ISSN (online) 1097-4164
    ISSN 1097-2765
    DOI 10.1016/j.molcel.2016.04.018
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: S6K1ing to ResTOR Adipogenesis with Polycomb

    Juan, Aster H / Vittorio Sartorelli

    Molecular cell. 2016 May 05, v. 62, no. 3

    2016  

    Abstract: Signal-directed chromatin recruitment of mammalian Polycomb complexes is a fundamental component of epigenetic regulation. In this issue, Yi et al. (2016) reveal how mTORC1 activation deploys the ribosomal serine/threonine kinase S6K1 and Polycomb ... ...

    Abstract Signal-directed chromatin recruitment of mammalian Polycomb complexes is a fundamental component of epigenetic regulation. In this issue, Yi et al. (2016) reveal how mTORC1 activation deploys the ribosomal serine/threonine kinase S6K1 and Polycomb proteins at genomic regulatory regions to repress expression of anti-adipogenic developmental regulators.
    Keywords adipogenesis ; chromatin ; epigenetics ; mammals ; protein-serine-threonine kinases ; serine ; threonine
    Language English
    Dates of publication 2016-0505
    Size p. 325-326.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 1415236-8
    ISSN 1097-4164 ; 1097-2765
    ISSN (online) 1097-4164
    ISSN 1097-2765
    DOI 10.1016/j.molcel.2016.04.018
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Protocol for RNA-seq library preparation starting from a rare muscle stem cell population or a limited number of mouse embryonic stem cells

    Stefania Dell’Orso / Aster H. Juan / Victoria Moiseeva / Laura García-Prat / Pura Muñoz-Cánoves / Vittorio Sartorelli

    STAR Protocols, Vol 2, Iss 2, Pp 100451- (2021)

    2021  

    Abstract: ... to Juan et al. (2016) and Garcia-Prat et al. (2016). ...

    Abstract Summary: It remains challenging to generate reproducible, high-quality cDNA libraries from RNA derived from rare cell populations. Here, we describe a protocol for high-throughput RNA-seq library preparation, including isolation of 200 skeletal muscle stem cells from mouse tibialis anterior muscle by fluorescence-activated cell sorting and cDNA preparation. We also describe RNA extraction and cDNA preparation from differentiating mouse embryonic stem cells.For complete details on the use and execution of this protocol, please refer to Juan et al. (2016) and Garcia-Prat et al. (2016).
    Keywords Cell isolation ; Flow Cytometry/Mass Cytometry ; RNA-seq ; Molecular Biology ; Stem Cells ; Cell Differentiation ; Science (General) ; Q1-390
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Protocol for RNA-seq library preparation starting from a rare muscle stem cell population or a limited number of mouse embryonic stem cells.

    Dell'Orso, Stefania / Juan, Aster H / Moiseeva, Victoria / García-Prat, Laura / Muñoz-Cánoves, Pura / Sartorelli, Vittorio

    STAR protocols

    2021  Volume 2, Issue 2, Page(s) 100451

    Abstract: ... to Juan et al. (2016) and Garcia-Prat et al. (2016). ...

    Abstract It remains challenging to generate reproducible, high-quality cDNA libraries from RNA derived from rare cell populations. Here, we describe a protocol for high-throughput RNA-seq library preparation, including isolation of 200 skeletal muscle stem cells from mouse tibialis anterior muscle by fluorescence-activated cell sorting and cDNA preparation. We also describe RNA extraction and cDNA preparation from differentiating mouse embryonic stem cells. For complete details on the use and execution of this protocol, please refer to Juan et al. (2016) and Garcia-Prat et al. (2016).
    MeSH term(s) Animals ; Flow Cytometry ; Gene Library ; Mice ; Mouse Embryonic Stem Cells/metabolism ; Myoblasts, Skeletal/metabolism ; RNA-Seq
    Language English
    Publishing date 2021-04-15
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2021.100451
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Correction: Single cell analysis of adult mouse skeletal muscle stem cells in homeostatic and regenerative conditions (doi: 10.1242/dev.174177).

    Dell'Orso, Stefania / Juan, Aster H / Ko, Kyung-Dae / Naz, Faiza / Perovanovic, Jelena / Gutierrez-Cruz, Gustavo / Feng, Xuesong / Sartorelli, Vittorio

    Development (Cambridge, England)

    2019  Volume 146, Issue 13

    Language English
    Publishing date 2019-06-28
    Publishing country England
    Document type Journal Article ; Published Erratum
    ZDB-ID 90607-4
    ISSN 1477-9129 ; 0950-1991
    ISSN (online) 1477-9129
    ISSN 0950-1991
    DOI 10.1242/dev.181743
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Polycomb Ezh1 maintains murine muscle stem cell quiescence through non-canonical regulation of Notch signaling.

    Feng, Xuesong / Wang, A Hongjun / Juan, Aster H / Ko, Kyung Dae / Jiang, Kan / Riparini, Giulia / Ciuffoli, Veronica / Kaba, Aissah / Lopez, Christopher / Naz, Faiza / Jarnik, Michal / Aliberti, Elizabeth / Hu, Shenyuan / Segalés, Jessica / Khateb, Mamduh / Acevedo-Luna, Natalia / Randazzo, Davide / Cheung, Tom H / Muñoz-Cánoves, Pura /
    Dell'Orso, Stefania / Sartorelli, Vittorio

    Developmental cell

    2023  Volume 58, Issue 12, Page(s) 1052–1070.e10

    Abstract: Organismal homeostasis and regeneration are predicated on committed stem cells that can reside for long periods in a mitotically dormant but reversible cell-cycle arrest state defined as quiescence. Premature escape from quiescence is detrimental, as it ... ...

    Abstract Organismal homeostasis and regeneration are predicated on committed stem cells that can reside for long periods in a mitotically dormant but reversible cell-cycle arrest state defined as quiescence. Premature escape from quiescence is detrimental, as it results in stem cell depletion, with consequent defective tissue homeostasis and regeneration. Here, we report that Polycomb Ezh1 confers quiescence to murine muscle stem cells (MuSCs) through a non-canonical function. In the absence of Ezh1, MuSCs spontaneously exit quiescence. Following repeated injuries, the MuSC pool is progressively depleted, resulting in failure to sustain proper muscle regeneration. Rather than regulating repressive histone H3K27 methylation, Ezh1 maintains gene expression of the Notch signaling pathway in MuSCs. Selective genetic reconstitution of the Notch signaling corrects stem cell number and re-establishes quiescence of Ezh1
    MeSH term(s) Mice ; Animals ; Cell Division ; Signal Transduction ; Cell Cycle Checkpoints ; Stem Cells ; Muscles
    Language English
    Publishing date 2023-04-26
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Intramural
    ZDB-ID 2054967-2
    ISSN 1878-1551 ; 1534-5807
    ISSN (online) 1878-1551
    ISSN 1534-5807
    DOI 10.1016/j.devcel.2023.04.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Sculpting chromatin beyond the double helix: epigenetic control of skeletal myogenesis.

    Sartorelli, Vittorio / Juan, Aster H

    Current topics in developmental biology

    2011  Volume 96, Page(s) 57–83

    Abstract: Satellite cells (SCs) are the main source of adult skeletal muscle stem cells responsible for muscle growth and regeneration. By interpreting extracellular cues, developmental regulators control quiescence, proliferation, and differentiation of SCs by ... ...

    Abstract Satellite cells (SCs) are the main source of adult skeletal muscle stem cells responsible for muscle growth and regeneration. By interpreting extracellular cues, developmental regulators control quiescence, proliferation, and differentiation of SCs by influencing coordinate gene expression. The scope of this review is limited to the description and discussion of protein complexes that introduce and decode heritable histone and chromatin modifications and how these modifications are relevant for SC biology.
    MeSH term(s) Animals ; Chromatin ; Epigenesis, Genetic ; Humans ; Muscle Development ; Muscle, Skeletal/cytology ; Muscle, Skeletal/growth & development ; Muscle, Skeletal/metabolism ; Transcription, Genetic
    Chemical Substances Chromatin
    Language English
    Publishing date 2011-04-29
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Intramural ; Review
    ISSN 1557-8933 ; 0070-2153
    ISSN (online) 1557-8933
    ISSN 0070-2153
    DOI 10.1016/B978-0-12-385940-2.00003-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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