LIVIVO - Search results -

Search results

Result 1 - 10 of total 452

Search options

Article ; Online: Viral PDZ Binding Motifs Influence Cell Behavior Through the Interaction with Cellular Proteins Containing PDZ Domains.

Castaño-Rodriguez, Carlos / Honrubia, Jose M / Gutiérrez-Álvarez, Javier / Sola, Isabel / Enjuanes, Luis

Methods in molecular biology (Clifton, N.J.)

2021 Volume 2256, Page(s) 217–236

Abstract: Viruses have evolved to interact with their hosts. Some viruses such as human papilloma virus, dengue virus, SARS-CoV, or influenza virus encode proteins including a PBM that interact with cellular proteins containing PDZ domains. There are more than 400 ...

Abstract	Viruses have evolved to interact with their hosts. Some viruses such as human papilloma virus, dengue virus, SARS-CoV, or influenza virus encode proteins including a PBM that interact with cellular proteins containing PDZ domains. There are more than 400 cellular protein isoforms with these domains in the human genome, indicating that viral PBMs have a high potential to influence the behavior of the cell. In this review we analyze the most relevant cellular processes known to be affected by viral PBM-cellular PDZ interactions including the establishment of cell-cell interactions and cell polarity, the regulation of cell survival and apoptosis and the activation of the immune system. Special attention has been provided to coronavirus PBM conservation throughout evolution and to the role of the PBMs of human coronaviruses SARS-CoV and MERS-CoV in pathogenesis.
MeSH term(s)	Apoptosis/physiology ; Cell Adhesion Molecules/metabolism ; Cell Proliferation/physiology ; Host-Pathogen Interactions ; Humans ; PDZ Domains ; Protein Binding ; Protein Structure, Secondary ; Viral Proteins/metabolism ; Virus Diseases/metabolism ; Virus Diseases/virology ; Viruses/isolation & purification ; Viruses/metabolism
Chemical Substances	Cell Adhesion Molecules ; Viral Proteins
Language	English
Publishing date	2021-05-20
Publishing country	United States
Document type	Journal Article ; Review
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-1166-1_13
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Book ; Online: Visual Semantic Navigation with Real Robots

Gutiérrez-Álvarez, Carlos / Ríos-Navarro, Pablo / Flor-Rodríguez, Rafael / Acevedo-Rodríguez, Francisco Javier / López-Sastre, Roberto J.

2023

Abstract: Visual Semantic Navigation (VSN) is the ability of a robot to learn visual semantic information for navigating in unseen environments. These VSN models are typically tested in those virtual environments where they are trained, mainly using reinforcement ... ...

Abstract	Visual Semantic Navigation (VSN) is the ability of a robot to learn visual semantic information for navigating in unseen environments. These VSN models are typically tested in those virtual environments where they are trained, mainly using reinforcement learning based approaches. Therefore, we do not yet have an in-depth analysis of how these models would behave in the real world. In this work, we propose a new solution to integrate VSN models into real robots, so that we have true embodied agents. We also release a novel ROS-based framework for VSN, ROS4VSN, so that any VSN-model can be easily deployed in any ROS-compatible robot and tested in a real setting. Our experiments with two different robots, where we have embedded two state-of-the-art VSN agents, confirm that there is a noticeable performance difference of these VSN solutions when tested in real-world and simulation environments. We hope that this research will endeavor to provide a foundation for addressing this consequential issue, with the ultimate aim of advancing the performance and efficiency of embodied agents within authentic real-world scenarios. Code to reproduce all our experiments can be found at https://github.com/gramuah/ros4vsn.
Keywords	Computer Science - Robotics ; Computer Science - Computer Vision and Pattern Recognition
Subject code	629
Publishing date	2023-11-28
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Genetically Engineered Live-Attenuated Middle East Respiratory Syndrome Coronavirus Viruses Confer Full Protection against Lethal Infection.

Gutiérrez-Álvarez, Javier / Honrubia, José M / Fernández-Delgado, Raúl / Wang, Li / Castaño-Rodríguez, Carlos / Zúñiga, Sonia / Sola, Isabel / Enjuanes, Luis

mBio

2021 Volume 12, Issue 2

Abstract: There are no approved vaccines against the life-threatening Middle East respiratory syndrome coronavirus (MERS-CoV). Attenuated vaccines have proven their potential to induce strong and long-lasting immune responses. We have previously described that ... ...

Abstract	There are no approved vaccines against the life-threatening Middle East respiratory syndrome coronavirus (MERS-CoV). Attenuated vaccines have proven their potential to induce strong and long-lasting immune responses. We have previously described that severe acute respiratory syndrome coronavirus (SARS-CoV) envelope (E) protein is a virulence factor. Based on this knowledge, a collection of mutants carrying partial deletions spanning the C-terminal domain of the E protein (rMERS-CoV-E) has been generated using a reverse genetics system. One of these mutants, MERS-CoV-EΔ2in, was attenuated and provided full protection in a challenge with virulent MERS-CoV after a single immunization dose. The MERS-CoV-E*Δ2in mutant was stable as it maintained its attenuation after 16 passages in cell cultures and has been selected as a promising vaccine candidate.
MeSH term(s)	COVID-19/immunology ; COVID-19/prevention & control ; Genetic Engineering/methods ; Humans ; Middle East Respiratory Syndrome Coronavirus/immunology ; Middle East Respiratory Syndrome Coronavirus/pathogenicity ; Vaccines, Attenuated/therapeutic use ; Viral Vaccines/therapeutic use
Chemical Substances	Vaccines, Attenuated ; Viral Vaccines
Language	English
Publishing date	2021-03-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mBio.00103-21
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Pellets/block bentonite barriers: Laboratory study of their evolution upon hydration

Villar, María Victoria / Iglesias, Rubén Javier / Gutiérrez-Álvarez, Carlos / Carbonell, Beatriz

Engineering geology. 2021 Oct., v. 292

2021

Abstract: Some deep geological nuclear waste disposal concepts are considering the installation around the waste canisters of bentonite barriers made up of two components: highly compacted blocks to support the canister and a granular buffer material consisting of ...

Abstract	Some deep geological nuclear waste disposal concepts are considering the installation around the waste canisters of bentonite barriers made up of two components: highly compacted blocks to support the canister and a granular buffer material consisting of bentonite pellets to fill the rest of the disposal gallery. The initial characteristics of these two components in terms of dry density, water content and microstructure are very different, but some, yet scarce, experimental evidence showed that saturation with groundwater will tend to homogenise the barrier. Nevertheless, the combined use of pellets and blocks in the same section of the barrier introduces difficulties in the understanding and modelling of the system performance that have to be addressed. With the aim of obtaining qualitative and quantitative information about the hydro-mechanical evolution of initially inhomogeneous bentonite samples (pellets/blocks) upon hydration under isothermal conditions, a series of laboratory tests in decimetre-scale cells was carried out. In most tests hydration took place through the pellets part, but the effect of hydrating through the block was also checked. As well, tests were performed either under low water injection pressure or under low water inflow rate.The results highlighted the role of the large macropores on the initial stages of hydration when this took place through the pellets, and also of the influence of water availability on its redistribution and on pressure build up. Pressure developed for lower degrees of saturation and water spread more homogeneously through the two sample components if hydration was slow. After full saturation differences in dry density across the two components remained, but they were softer if saturation took place at a slow pace, either because of the low water inflow rate or because it occurred through the less permeable block. The microstructure of the bentonite in the two components was very different even after full saturation: although there was an overall trend to pore size homogenisation towards smaller sizes over time, the ratio of microstructural void ratio to macrostructural void ratio kept lower in the pellets than in the block. As well, the number of water layers in the interlayer was higher in pellets samples than in block samples.The research also highlighted the importance of the right assessment of macroporosity (particularly pores larger than 550 μm) from mercury intrusion porosimetry tests and the likely effect of local density on the pressure measured.
Keywords	bentonite ; evolution ; groundwater ; homogenization ; macropores ; mercury ; microstructure ; porosimetry ; porosity ; radioactive waste ; waste disposal ; water content
Language	English
Dates of publication	2021-10
Publishing place	Elsevier B.V.
Document type	Article
ISSN	0013-7952
DOI	10.1016/j.enggeo.2021.106272
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Children's comparison of different-length numbers: Managing different attributes in multidigit number processing.

García-Orza, Javier / Gutiérrez-Cordero, Ismael / Rodríguez-Montenegro, Ismael / Álvarez-Montesinos, Juan Antonio

Journal of experimental child psychology

2024 Volume 240, Page(s) 105827

Abstract: In everyday life the comparison of numbers usually occurs between numbers with different numbers of digits. However, experimental research here is scarce. Recent research has shown that adults respond faster to congruent pairs (the initial digit in the ... ...

Abstract	In everyday life the comparison of numbers usually occurs between numbers with different numbers of digits. However, experimental research here is scarce. Recent research has shown that adults respond faster to congruent pairs (the initial digit in the number with more digits is larger, e.g., 2384 vs. 107) than to incongruent pairs (the initial digit is larger in the number with fewer digits, e.g., 2675 vs. 398). This has been interpreted as support for the processing of multiple attributes in parallel and against serial accounts. The current research asked whether there is a change in the relevance of these attributes as school grades increase. School-age children from the second to sixth grades (N = 206) were presented with pairs of numbers that had either the same number of digits (3 vs. 3 or 4 vs. 4) or a different number of digits (3 vs. 4). In this latter condition, the stimuli, matched by distance, could be either length/digit congruent (e.g., 2384 vs. 107) or length/digit incongruent (e.g., 2675 vs. 398). Linear mixed models showed a length/digit congruity effect from second graders. Interestingly, in the response time measure, congruity interacted with school grade and the side in which the larger number of the pair was presented. Whereas these results support a model that considers number comparison as a process that weighs different attributes in parallel, it is also argued that developmental changes are associated with differences in the level of automatization of the componential skills involved in the comparison.
MeSH term(s)	Child ; Humans ; Reaction Time ; Mathematical Concepts
Language	English
Publishing date	2024-01-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218137-x
ISSN	1096-0457 ; 0022-0965
ISSN (online)	1096-0457
ISSN	0022-0965
DOI	10.1016/j.jecp.2023.105827
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 670: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Necesidades no cubiertas en asma alérgica grave.

Delgado, Julio / Navarro, Ana / Álvarez-Gutiérrez, Francisco Javier / Cisneros, Carolina / Domínguez-Ortega, Javier

Open respiratory archives

2023 Volume 5, Issue 4, Page(s) 100282

Abstract: Severe asthma affects 3%-10% of the world's population, according to estimates by the Global Initiative for ASTHMA (GINA). Allergic asthma is one of the most common phenotypes of severe asthma and it is characterized by allergen-induced type 2 ... ...

Title translation	Unmet Needs in Severe Allergic Asthma.
Abstract	Severe asthma affects 3%-10% of the world's population, according to estimates by the Global Initiative for ASTHMA (GINA). Allergic asthma is one of the most common phenotypes of severe asthma and it is characterized by allergen-induced type 2 inflammation in which immunoglobulin E (IgE) is a key mediator, making it an important therapeutic target. The introduction of targeted biological therapies or treatments has entered the management for severe asthma in the era of precision medicine, and the goal of treatment is clinical remission of the disease. There is a significant percentage of patients with severe allergic asthma who do not respond to treatments and whose symptoms are not controlled. In this paper, a group of experts in the management of severe allergic asthma reviewed and evaluated the most relevant evidence regarding the pathophysiology and phenotypes of severe allergic asthma, the role of IgE in allergic inflammation, allergen identification, techniques, biomarkers and diagnostic challenges, available treatments and strategies for disease management, with a special focus on biological treatments. From this review, recommendations were developed and validated through a Delphi consensus process with the aim of offering improvements in the management of severe allergic asthma to the professionals involved and identifying the unmet needs in the management of this pathology.
Language	Spanish
Publishing date	2023-11-01
Publishing country	Spain
Document type	English Abstract ; Journal Article
ISSN	2659-6636
ISSN (online)	2659-6636
DOI	10.1016/j.opresp.2023.100282
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Asthma Exists in Older People too.

Perpiñá Tordera, Miguel / Álvarez Gutiérrez, Francisco Javier / Blanco Aparicio, Marina

Archivos de bronconeumologia

2022 Volume 58, Issue 5, Page(s) 390–391

Title translation	El asma en las personas mayores también existe.
MeSH term(s)	Aged ; Anti-Asthmatic Agents/therapeutic use ; Asthma/drug therapy ; Humans
Chemical Substances	Anti-Asthmatic Agents
Language	Spanish
Publishing date	2022-03-11
Publishing country	Spain
Document type	Editorial
ZDB-ID	733126-5
ISSN	1579-2129 ; 0300-2896
ISSN (online)	1579-2129
ISSN	0300-2896
DOI	10.1016/j.arbres.2022.02.010
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2655: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Correction for Castaño-Rodriguez et al., "Role of Severe Acute Respiratory Syndrome Coronavirus Viroporins E, 3a, and 8a in Replication and Pathogenesis".

Castaño-Rodríguez, Carlos / Honrubia, Jose M / Gutierrez-Álvarez, Javier / DeDiego, Marta L / Nieto-Torres, Jose L / Jimenez-Guardeño, Jose M / Regla-Nava, Jose A / Fernandez-Delgado, Raul / Verdia-Báguena, Carmina / Queralt-Martín, Maria / Kochan, Grazyna / Perlman, Stanley / Aguilella, Vicente M / Sola, Isabel / Enjuanes, Luis

mBio

2023 , Page(s) e0248523

Language	English
Publishing date	2023-11-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mbio.02485-23
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: SARS-CoV-2-Mediated Lung Edema and Replication Are Diminished by Cystic Fibrosis Transmembrane Conductance Regulator Modulators.

Honrubia, Jose M / Gutierrez-Álvarez, Javier / Sanz-Bravo, Alejandro / González-Miranda, Ezequiel / Muñoz-Santos, Diego / Castaño-Rodriguez, Carlos / Wang, Li / Villarejo-Torres, Marta / Ripoll-Gómez, Jorge / Esteban, Ana / Fernandez-Delgado, Raul / Sánchez-Cordón, Pedro José / Oliveros, Juan Carlos / Perlman, Stanley / McCray, Paul B / Sola, Isabel / Enjuanes, Luis

mBio

2023 Volume 14, Issue 1, Page(s) e0313622

Abstract: Coronaviruses (CoVs) of genera α, β, γ, and δ encode proteins that have a PDZ-binding motif (PBM) consisting of the last four residues of the envelope (E) protein (PBM core). PBMs may bind over 400 cellular proteins containing PDZ domains (an acronym ... ...

Abstract	Coronaviruses (CoVs) of genera α, β, γ, and δ encode proteins that have a PDZ-binding motif (PBM) consisting of the last four residues of the envelope (E) protein (PBM core). PBMs may bind over 400 cellular proteins containing PDZ domains (an acronym formed by the combination of the first letter of the names of the three first proteins where this domain was identified), making them relevant for the control of cell function. Three highly pathogenic human CoVs have been identified to date: severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV), and SARS-CoV-2. The PBMs of the three CoVs were virulence factors. SARS-CoV mutants in which the E protein PBM core was replaced by the E protein PBM core from virulent or attenuated CoVs were constructed. These mutants showed a gradient of virulence, depending on whether the alternative PBM core introduced was derived from a virulent or an attenuated CoV. Gene expression patterns in the lungs of mice infected with SARS-CoVs encoding each of the different PBMs were analyzed by RNA sequencing of infected lung tissues. E protein PBM of SARS-CoV and SARS-CoV-2 dysregulated gene expression related to ion transport and cell homeostasis. Decreased expression of cystic fibrosis transmembrane conductance regulator (CFTR) mRNA, essential for alveolar edema resolution, was shown. Reduced CFTR mRNA levels were associated with edema accumulation in the alveoli of mice infected with SARS-CoV and SARS-CoV-2. Compounds that increased CFTR expression and activity, significantly reduced SARS-CoV-2 growth in cultured cells and protected against mouse infection, suggesting that E protein virulence is mediated by a decreased CFTR expression.
MeSH term(s)	Animals ; Mice ; Humans ; SARS-CoV-2/genetics ; Cystic Fibrosis Transmembrane Conductance Regulator/genetics ; Severe acute respiratory syndrome-related coronavirus/genetics ; COVID-19 ; Virulence Factors/genetics ; Virulence Factors/metabolism ; Middle East Respiratory Syndrome Coronavirus/genetics ; Lung/metabolism ; RNA, Messenger
Chemical Substances	Cystic Fibrosis Transmembrane Conductance Regulator (126880-72-6) ; Virulence Factors ; RNA, Messenger
Language	English
Publishing date	2023-01-10
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mbio.03136-22
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Autophagy sustains mitochondrial respiration and determines resistance to BRAF

Díaz-Gago, Sergio / Vicente-Gutiérrez, Javier / Ruiz-Rodríguez, Jose Manuel / Calafell, Josep / Álvarez-Álvarez, Alicia / Lasa, Marina / Chiloeches, Antonio / Baquero, Pablo

Autophagy

2024 , Page(s) 1–15

Abstract: ... ...

Abstract	BRAF
Language	English
Publishing date	2024-03-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	2454135-7
ISSN	1554-8635 ; 1554-8627
ISSN (online)	1554-8635
ISSN	1554-8627
DOI	10.1080/15548627.2024.2312790
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 6741: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito