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Article ; Online: Structural and Functional Characteristics of the Proline-Rich Antimicrobial Peptide Minibactenecin from Leukocytes of Domestic Goat Capra hircus.

Bolosov, I A / Panteleev, P V / Balandin, S V / Shamova, O V / Ovchinnikova, T V

Bulletin of experimental biology and medicine

2023 Volume 174, Issue 4, Page(s) 440–445

Abstract: We performed structural and functional studies of minibactenecin mini-ChBac7.5Nα, a natural proline-rich cathelicidin from domestic goat Capra hircus. To identify the key residues important for the biological action of the peptide, a panel of its alanine- ...

Abstract	We performed structural and functional studies of minibactenecin mini-ChBac7.5Nα, a natural proline-rich cathelicidin from domestic goat Capra hircus. To identify the key residues important for the biological action of the peptide, a panel of its alanine-substituted analogues was produced. The development of E. coli resistance to the natural minibactenecin, as well as to its analogues carrying substitutions for hydrophobic amino acids in the C-terminal residues was studied. The data obtained indicate the possibility of rapid development of the resistance to this class of peptides. The main factors in the formation of the antibiotic resistance are various mutations leading to inactivation of the SbmA transporter.
MeSH term(s)	Animals ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Antimicrobial Peptides ; Proline/pharmacology ; Peptides/metabolism ; Leukocytes/metabolism ; Goats/genetics ; Goats/metabolism
Chemical Substances	Antimicrobial Peptides ; Proline (9DLQ4CIU6V) ; Peptides
Language	English
Publishing date	2023-03-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	390407-6
ISSN	1573-8221 ; 0007-4888 ; 0365-9615
ISSN (online)	1573-8221
ISSN	0007-4888 ; 0365-9615
DOI	10.1007/s10517-023-05725-1
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Article: Erratum to: Experimental Search for New Means of Pathogenetic Therapy COVID-19: Inhibitor of H2-Receptors Famotidine Increases the Effect of Oseltamivir on Survival and Immune Status of Mice Infected by A/PR/8/34 (H1N1).

Goncharov, N V / Vasilyev, K A / Kudryavtsev, I V / Avdonin, P P / Belinskaia, D A / Stukova, M A / Shamova, O V / Avdonin, P V

Journal of evolutionary biochemistry and physiology

2022 Volume 58, Issue 2, Page(s) 623

Abstract: This corrects the article DOI: 10.1134/S0022093022010203.]. ...

Abstract	[This corrects the article DOI: 10.1134/S0022093022010203.].
Language	English
Publishing date	2022-05-16
Publishing country	United States
Document type	Published Erratum
ZDB-ID	201501-8
ISSN	1608-3202 ; 0022-0930
ISSN (online)	1608-3202
ISSN	0022-0930
DOI	10.1134/S0022093022020284
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Article: Experimental Search for New Means of Pathogenetic Therapy COVID-19: Inhibitor of H2-Receptors Famotidine Increases the Effect of Oseltamivir on Survival and Immune Status of Mice Infected by A/PR/8/34 (H1N1).

Goncharov, N V / Vasilyev, K A / Kudryavtsev, I V / Avdonin, P P / Belinskaia, D A / Stukova, M A / Shamova, O V / Avdonin, P V

Journal of evolutionary biochemistry and physiology

2022 Volume 58, Issue 1, Page(s) 230–246

Abstract: The development of drugs for the therapy of COVID-19 is one of the main problems of modern physiology, biochemistry and pharmacology. Taking into account the available information on the participation of mast cells and the role of histamine in the ... ...

Abstract	The development of drugs for the therapy of COVID-19 is one of the main problems of modern physiology, biochemistry and pharmacology. Taking into account the available information on the participation of mast cells and the role of histamine in the pathogenesis of COVID-19, as well as information on the positive role of famotidine in the prevention and treatment of coronavirus infection, an experiment was carried out using famotidine in a mouse model. We used a type A/PR/8/34 (H1N1) virus adapted to mice. The antiviral drug oseltamivir (Tamiflu), which belongs to the group of neuraminidase inhibitors, was used as a reference drug. The use of famotidine in combination with oseltamivir can increase survival, improve the dynamics of animal weight, reduce the level of NKT cells and increase the level of naive T-helpers. Further studies of famotidine in vivo should be aimed at optimizing the regimen of drug use at a higher viral load, as well as with a longer use of famotidine.
Language	English
Publishing date	2022-03-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	201501-8
ISSN	1608-3202 ; 0022-0930
ISSN (online)	1608-3202
ISSN	0022-0930
DOI	10.1134/S0022093022010203
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Article ; Online: First vertebrate BRICHOS antimicrobial peptides: β-hairpin host defense peptides in limbless amphibia lung resemble those of marine worms.

Smetanin, Ruslan V / Sukhareva, Maria S / Vladimirova, Elizaveta V / Zharkova, Maria S / Mikushina, Anna D / Komlev, Aleksey S / Khaydukova, Maria M / Filatenkova, Tatiana A / Kalganova, Anastasia I / Pipiya, Sofiya O / Terekhov, Stanislav S / Orlov, Dmitriy S / Shamova, Olga V / Eliseev, Igor E

Biochemical and biophysical research communications

2024 Volume 712-713, Page(s) 149913

Abstract: Innate immunity of invertebrates offers potent antimicrobial peptides (AMPs) against drug-resistant infections. To identify new worm β-hairpin AMPs, we explored the sequence diversity of proteins with a BRICHOS domain, which comprises worm AMP precursors. ...

Abstract	Innate immunity of invertebrates offers potent antimicrobial peptides (AMPs) against drug-resistant infections. To identify new worm β-hairpin AMPs, we explored the sequence diversity of proteins with a BRICHOS domain, which comprises worm AMP precursors. Strikingly, we discovered new BRICHOS AMPs not in worms, but in caecilians, the least studied clade of vertebrates. Two precursor proteins from Microcaecilia unicolor and Rhinatrema bivittatum resemble SP-C lung surfactants and bear worm AMP-like peptides at C-termini. The analysis of M. unicolor tissue transcriptomes shows that the AMP precursor is highly expressed in the lung along with regular SP-C, suggesting a different, protective function. The peptides form right-twisted β-hairpins, change conformation upon lipid binding, and rapidly disrupt bacterial membranes. Both peptides exhibit broad-spectrum activity against multidrug-resistant ESKAPE pathogens with 1-4 μM MICs and remarkably low toxicity, giving 40-70-fold selectivity towards bacteria. These BRICHOS AMPs, previously unseen in vertebrates, reveal a novel lung innate immunity mechanism and offer a promising antibiotics template.
Language	English
Publishing date	2024-04-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	205723-2
ISSN	1090-2104 ; 0006-291X ; 0006-291X
ISSN (online)	1090-2104 ; 0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2024.149913
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Article: Creation of Recombinant Biocontrol Agents by Genetic Programming of Yeast.

Pipiya, S O / Mirzoeva, N Z / Baranova, M N / Eliseev, I E / Mokrushina, Yu A / Shamova, O V / Gabibov, A G / Smirnov, I V / Terekhov, S S

Acta naturae

2023 Volume 15, Issue 1, Page(s) 74–80

Abstract: Bacterial infections caused by antibiotic-resistant pathogens pose an extremely serious and elusive problem in healthcare. The discovery and targeted creation of new antibiotics are today among the most important public health issues. Antibiotics based ... ...

Abstract	Bacterial infections caused by antibiotic-resistant pathogens pose an extremely serious and elusive problem in healthcare. The discovery and targeted creation of new antibiotics are today among the most important public health issues. Antibiotics based on antimicrobial peptides (AMPs) are of particular interest due to their genetically encoded nature. A distinct advantage of most AMPs is their direct mechanism of action that is mediated by their membranolytic properties. The low rate of emergence of antibiotic resistance associated with the killing mechanism of action of AMPs attracts heightened attention to this field. Recombinant technologies enable the creation of genetically programmable AMP producers for large-scale generation of recombinant AMPs (rAMPs) or the creation of rAMP-producing biocontrol agents. The methylotrophic yeast Pichia pastoris was genetically modified for the secreted production of rAMP. Constitutive expression of the sequence encoding the mature AMP protegrin-1 provided the yeast strain that effectively inhibits the growth of target gram-positive and gram-negative bacteria. An antimicrobial effect was also observed in the microculture when a yeast rAMP producer and a reporter bacterium were co-encapsulated in droplets of microfluidic double emulsion. The heterologous production of rAMPs opens up new avenues for creating effective biocontrol agents and screening antimicrobial activity using ultrahigh-throughput technologies.
Language	English
Publishing date	2023-05-08
Publishing country	Russia (Federation)
Document type	Journal Article
ISSN	2075-8251
ISSN	2075-8251
DOI	10.32607/actanaturae.11878
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Article: Bioengineering the Antimicrobial Activity of Yeast by Recombinant Thanatin Production.

Pipiya, Sofiya O / Kudzhaev, Arsen M / Mirzoeva, Nisso Z / Mokrushina, Yuliana A / Ziganshin, Rustam H / Komlev, Alexey S / Petrova, Polina E / Smirnov, Ivan V / Gabibov, Alexander G / Shamova, Olga V / Terekhov, Stanislav S

Antibiotics (Basel, Switzerland)

2023 Volume 12, Issue 12

Abstract: The global spread of antibiotic resistance marks the end of the era of conventional antibiotics. Mankind desires new molecular tools to fight pathogenic bacteria. In this regard, the development of new antimicrobials based on antimicrobial peptides (AMPs) ...

Abstract	The global spread of antibiotic resistance marks the end of the era of conventional antibiotics. Mankind desires new molecular tools to fight pathogenic bacteria. In this regard, the development of new antimicrobials based on antimicrobial peptides (AMPs) is again of particular interest. AMPs have various mechanisms of action on bacterial cells. Moreover, AMPs have been reported to be efficient in preclinical studies, demonstrating a low level of resistance formation. Thanatin is a small, beta-hairpin antimicrobial peptide with a bacterial-specific mode of action, predetermining its low cytotoxicity toward eukaryotic cells. This makes thanatin an exceptional candidate for new antibiotic development. Here, a microorganism was bioengineered to produce an antimicrobial agent, providing novel opportunities in antibiotic research through the directed creation of biocontrol agents. The constitutive heterologous production of recombinant thanatin (rThan) in the yeast
Language	English
Publishing date	2023-12-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2681345-2
ISSN	2079-6382
ISSN	2079-6382
DOI	10.3390/antibiotics12121719
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Article ; Online: Structure-biological activity relationships of myeloperoxidase to effect on platelet activation.

Gorudko, I V / Grigorieva, D V / Shamova, E V / Gorbunov, N P / Kokhan, A U / Kostevich, V A / Vasilyev, V B / Panasenko, O M / Khinevich, N V / Bandarenka, H V / Burko, A A / Sokolov, A V

Archives of biochemistry and biophysics

2022 Volume 728, Page(s) 109353

Abstract: Myeloperoxidase (MPO), an oxidant-producing enzyme of neutrophils, has been shown to prime platelet activity promoting immunothrombosis. Native MPO is a homodimer, consisting of two identical protomers (monomer) connected by a single disulfide bond. But ... ...

Abstract	Myeloperoxidase (MPO), an oxidant-producing enzyme of neutrophils, has been shown to prime platelet activity promoting immunothrombosis. Native MPO is a homodimer, consisting of two identical protomers (monomer) connected by a single disulfide bond. But in inflammatory foci, MPO can be found both in the form of a monomer and in the form of a dimer. Beside MPO can also be in complexes with other molecules and be modified by oxidants, which ultimately affect its physicochemical properties and functions. Here we compared the effects of various forms of MPO as well as MPO in complex with ceruloplasmin (CP), a physiological inhibitor of MPO, on the platelet activity. Monomeric MPO (hemi-MPO) was obtained by treating the dimeric MPO by reductive alkylation. MPO was modified with HOCl in a molar ratio of 1:100 (MPO-HOCl). Using surface-enhanced Raman scattering (SERS) spectroscopy we showed that peaks at about 510 and 526 cm
MeSH term(s)	Coloring Agents ; Disulfides ; Hypochlorous Acid ; Neutrophils ; Oxidants ; Peroxidase ; Platelet Activation
Chemical Substances	Coloring Agents ; Disulfides ; Oxidants ; Hypochlorous Acid (712K4CDC10) ; Peroxidase (EC 1.11.1.7)
Language	English
Publishing date	2022-07-16
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	523-x
ISSN	1096-0384 ; 0003-9861
ISSN (online)	1096-0384
ISSN	0003-9861
DOI	10.1016/j.abb.2022.109353
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Article ; Online: A Simple Contactless High-Frequency Electromagnetic Sensor: Proof of Concept.

Yuskina, Ekaterina / Makarov, Nikodim / Khaydukova, Maria / Filatenkova, Tatiana / Shamova, Olga / Semenov, Valentin / Panchuk, Vitaly / Kirsanov, Dmitry

Analytical chemistry

2022 Volume 94, Issue 35, Page(s) 11978–11982

Abstract: We report on the development of a very simple and inexpensive sensor device based on an inductance coil connected to a high-frequency electric field generator. The working principle of this sensor is as follows: liquid sample in a plastic tube is placed ... ...

Abstract	We report on the development of a very simple and inexpensive sensor device based on an inductance coil connected to a high-frequency electric field generator. The working principle of this sensor is as follows: liquid sample in a plastic tube is placed inside the inductance coil as its core and this core changes the properties of high-frequency electric current passing through the coil; these changes depend on sample conductivity, dielectric constant, magnetic properties, and capacitance. The electric signal registered after the coil represents a kind of a spectrum that can be effectively treated with chemometric tools. Our studies have shown that such a sensor can be used to distinguish between substances with different physical and chemical properties; different concentrations of ions in aqueous solutions with a linear response in a broad concentration range from 10
MeSH term(s)	Electric Capacitance ; Electric Conductivity ; Electricity ; Magnetics ; Water
Chemical Substances	Water (059QF0KO0R)
Language	English
Publishing date	2022-08-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.2c02067
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Article: Structure-biological activity relationships of myeloperoxidase to effect on platelet activation

Gorudko, I.V. / Grigorieva, D.V. / Shamova, E.V. / Gorbunov, N.P. / Kokhan, A.U. / Kostevich, V.A. / Vasilyev, V.B. / Panasenko, O.M. / Khinevich, N.V. / Bandarenka, H.V. / Burko, A.A. / Sokolov, A.V.

Archives of biochemistry and biophysics. 2022 July 11,

2022

Abstract	Myeloperoxidase (MPO), an oxidant-producing enzyme of neutrophils, has been shown to prime platelet activity promoting immunothrombosis. Native MPO is a homodimer, consisting of two identical protomers (monomer) connected by a single disulfide bond. But in inflammatory foci, MPO can be found both in the form of a monomer and in the form of a dimer. Beside MPO can also be in complexes with other molecules and be modified by oxidants, which ultimately affect its physicochemical properties and functions. Here we compared the effects of various forms of MPO as well as MPO in complex with ceruloplasmin (CP), a physiological inhibitor of MPO, on the platelet activity. Monomeric MPO (hemi-MPO) was obtained by treating the dimeric MPO by reductive alkylation. MPO was modified with HOCl in a molar ratio of 1:100 (MPO-HOCl). Using surface-enhanced Raman scattering (SERS) spectroscopy we showed that peaks at about 510 and 526 cm⁻¹ corresponded to disulfide bond was recognizable in the SERS-spectra of dimeric MPO, absent in the spectrum of hemi-MPO and less intense in the spectra of MPO-HOCl, which indicates the partial decomposition of dimeric MPO with a disulfide bond cleavage under the HOCl modification. It was shown hemi-MPO to a lesser extent than dimeric MPO bound to platelets and enhanced their agonist-induced aggregation and platelet-neutrophil aggregate formation. MPO modified by HOCl and MPO in complex with CP did not bind to platelets and have no effect on platelet activity. Thus, the modification of MPO by HOCl, its presence in monomeric form as well as in complex with CP reduces MPO effect on platelet function and consequently decreases the risk of thrombosis in inflammatory foci.
Keywords	alkylation ; biophysics ; cleavage (chemistry) ; disulfide bonds ; ferroxidase ; myeloperoxidase ; neutrophils ; platelet activation ; protein subunits ; risk ; thrombosis
Language	English
Dates of publication	2022-0711
Publishing place	Elsevier Inc.
Document type	Article
Note	Pre-press version
ZDB-ID	523-x
ISSN	1096-0384 ; 0003-9861
ISSN (online)	1096-0384
ISSN	0003-9861
DOI	10.1016/j.abb.2022.109353
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Indolicidin analogs with broad-spectrum antimicrobial activity and low hemolytic activity.

Smirnova, M P / Kolodkin, N I / Kolobov, A A / Afonin, V G / Afonina, I V / Stefanenko, L I / Shpen', V M / Shamova, O V

Peptides

2020 Volume 132, Page(s) 170356

Abstract: To create a broad-spectrum peptide biocide, we synthesized 45 analogs of antimicrobial peptide indolicidin (H-Ile-Leu-Pro-Trp-Lys-Trp-Pro-Trp-Trp-Pro-Trp-Arg-Arg- ... ...

Abstract	To create a broad-spectrum peptide biocide, we synthesized 45 analogs of antimicrobial peptide indolicidin (H-Ile-Leu-Pro-Trp-Lys-Trp-Pro-Trp-Trp-Pro-Trp-Arg-Arg-NH
MeSH term(s)	Amino Acid Sequence ; Anti-Infective Agents/chemical synthesis ; Anti-Infective Agents/chemistry ; Anti-Infective Agents/pharmacology ; Antimicrobial Cationic Peptides/chemical synthesis ; Antimicrobial Cationic Peptides/chemistry ; Antimicrobial Cationic Peptides/pharmacology ; Drug Design ; Gram-Negative Bacteria/drug effects ; Gram-Positive Bacteria/drug effects ; Hemolysis/drug effects ; Humans ; Microbial Sensitivity Tests ; Peptides/chemical synthesis ; Structure-Activity Relationship ; Yeasts/drug effects
Chemical Substances	Anti-Infective Agents ; Antimicrobial Cationic Peptides ; Peptides ; indolicidin (073SBV429N)
Language	English
Publishing date	2020-06-25
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	769028-9
ISSN	1873-5169 ; 0196-9781
ISSN (online)	1873-5169
ISSN	0196-9781
DOI	10.1016/j.peptides.2020.170356
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