LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 131

Search options

  1. Article ; Online: Mining the Antibody Repertoire for Solutions to SARS-CoV-2.

    Meng, Wenzhao / Rosenfeld, Aaron M / Luning Prak, Eline T

    Cell host & microbe

    2020  Volume 28, Issue 4, Page(s) 499–501

    Abstract: In this issue of Cell Host & Microbe, Nielsen and colleagues sequence antibody repertoires of patients with severe COVID-19 to reveal potentially convergent features on the background of a larger, polyclonal response. Their findings suggest that, as ... ...

    Abstract In this issue of Cell Host & Microbe, Nielsen and colleagues sequence antibody repertoires of patients with severe COVID-19 to reveal potentially convergent features on the background of a larger, polyclonal response. Their findings suggest that, as databases improve, it may be possible to monitor virus-specific B cells after infection or vaccination using antibody sequencing.
    MeSH term(s) Antibodies, Viral ; Antibody Formation ; B-Lymphocytes ; Betacoronavirus ; COVID-19 ; Coronavirus Infections ; Humans ; Immunoglobulin G ; Pandemics ; Pneumonia, Viral ; SARS-CoV-2 ; Severe Acute Respiratory Syndrome
    Chemical Substances Antibodies, Viral ; Immunoglobulin G
    Keywords covid19
    Language English
    Publishing date 2020-10-07
    Publishing country United States
    Document type Journal Article ; Comment
    ZDB-ID 2278004-X
    ISSN 1934-6069 ; 1931-3128
    ISSN (online) 1934-6069
    ISSN 1931-3128
    DOI 10.1016/j.chom.2020.09.010
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6578: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Characterization of three succinyl-CoA acyltransferases involved in polyketide chain assembly.

    Liu, Lilu / Wang, Wenzhao / Chen, Meng / Zhang, Yuwei / Mao, Huijin / Wang, Dacheng / Chen, Yihua / Li, Pengwei

    Applied microbiology and biotechnology

    2023  Volume 107, Issue 7-8, Page(s) 2403–2412

    Abstract: Polyketides are a class of natural products with astonishing structural diversities, fascinating biological activities, and a versatile of applications. In polyketides biosynthesis, acyltransferases (ATs) are the 'gatekeeping' enzymes selecting the ... ...

    Abstract Polyketides are a class of natural products with astonishing structural diversities, fascinating biological activities, and a versatile of applications. In polyketides biosynthesis, acyltransferases (ATs) are the 'gatekeeping' enzymes selecting the specific CoA-activated acyl groups as building blocks and transferring them onto the phosphopantetheine arm of acyl carrier proteins (ACPs) to enable the following condensation reactions to assemble the polyketide chain. Herein, the Art2 protein from aurantinins, a group of the antibacterial polyketides, is characterized in vitro as an AT that can load a CoA-activated succinyl unit onto the first ACP domain of Art17 (ACP
    MeSH term(s) Acyltransferases/metabolism ; Polyketides/metabolism ; Acyl Coenzyme A/metabolism ; Anti-Bacterial Agents ; Polyketide Synthases/metabolism
    Chemical Substances succinyl-coenzyme A (BSI27HW5EQ) ; Acyltransferases (EC 2.3.-) ; Polyketides ; Acyl Coenzyme A ; Anti-Bacterial Agents ; Polyketide Synthases (79956-01-7)
    Language English
    Publishing date 2023-03-16
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-023-12481-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2229: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Mining the Antibody Repertoire for Solutions to SARS-CoV-2

    Meng, Wenzhao / Rosenfeld, Aaron M. / Luning Prak, Eline T.

    Cell Host & Microbe

    2020  Volume 28, Issue 4, Page(s) 499–501

    Keywords Microbiology ; Parasitology ; Virology ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    ZDB-ID 2278004-X
    ISSN 1931-3128
    ISSN 1931-3128
    DOI 10.1016/j.chom.2020.09.010
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6578: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: SARS-CoV-2 Spike-Specific T-Cell Responses in Patients With B-Cell Depletion Who Received Chimeric Antigen Receptor T-Cell Treatments.

    Parvathaneni, Kalpana / Torres-Rodriguez, Kyabeth / Meng, Wenzhao / Hwang, Wei-Ting / Frey, Noelle / Naji, Ali / Bhoj, Vijay G

    JAMA oncology

    2022  Volume 8, Issue 1, Page(s) 164–167

    MeSH term(s) B-Lymphocytes ; COVID-19/immunology ; Humans ; Immunotherapy, Adoptive ; Receptors, Chimeric Antigen ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus/immunology ; T-Lymphocytes/immunology
    Chemical Substances Receptors, Chimeric Antigen ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2022-01-20
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2374-2445
    ISSN (online) 2374-2445
    DOI 10.1001/jamaoncol.2021.6030
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: Characterization of three succinyl-CoA acyltransferases involved in polyketide chain assembly

    Liu, Lilu / Wang, Wenzhao / Chen, Meng / Zhang, Yuwei / Mao, Huijin / Wang, Dacheng / Chen, Yihua / Li, Pengwei

    Appl Microbiol Biotechnol. 2023 Apr., v. 107, no. 7-8 p.2403-2412

    2023  

    Abstract: Polyketides are a class of natural products with astonishing structural diversities, fascinating biological activities, and a versatile of applications. In polyketides biosynthesis, acyltransferases (ATs) are the ‘gatekeeping’ enzymes selecting the ... ...

    Abstract Polyketides are a class of natural products with astonishing structural diversities, fascinating biological activities, and a versatile of applications. In polyketides biosynthesis, acyltransferases (ATs) are the ‘gatekeeping’ enzymes selecting the specific CoA-activated acyl groups as building blocks and transferring them onto the phosphopantetheine arm of acyl carrier proteins (ACPs) to enable the following condensation reactions to assemble the polyketide chain. Herein, the Art2 protein from aurantinins, a group of the antibacterial polyketides, is characterized in vitro as an AT that can load a CoA-activated succinyl unit onto the first ACP domain of Art17 (ACPAᵣₜ₁₇₋₁). In addition, another two proteins, GbnB and EtnB, involved in the biosynthesis of gladiolin and etnangien respectively, were traced by literature mining, homologous searching, and product structure analysis and then identified as functional succinyl-CoA ATs by the ACPAᵣₜ₁₇₋₁ assays. Taken together, by the assay method employing ACPAᵣₜ₁₇₋₁ as an acyl acceptor, we identified three ATs that can introduce a succinyl unit into the polyketide assembly line, which enriches the toolbox of polyketide biosynthetic enzymes and sets a stage for incorporating a succinyl unit into polyketide backbones in synthetic biological manners. KEY POINTS: • Three acyltransferases that are able to load ACP with a succinyl unit were characterized in vitro. • ACPAᵣₜ₁₇₋₁ can be used as an acceptor to assay succinyl-CoA AT from different polyketides. • The succinyl unit can be incorporated into polyketides assembly process.
    Keywords acyltransferases ; biosynthesis ; polyketides
    Language English
    Dates of publication 2023-04
    Size p. 2403-2412.
    Publishing place Springer Berlin Heidelberg
    Document type Article ; Online
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-023-12481-9
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 79/727: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article ; Online: Neutrophil Activity and Extracellular Matrix Degradation: Drivers of Lung Tissue Destruction in Fatal COVID-19 Cases and Implications for Long COVID.

    Narasaraju, Teluguakula / Neeli, Indira / Criswell, Sheila L / Krishnappa, Amita / Meng, Wenzhao / Silva, Vasuki / Bila, Galyna / Vovk, Volodymyr / Serhiy, Zolotukhin / Bowlin, Gary L / Meyer, Nuala / Luning Prak, Eline T / Radic, Marko / Bilyy, Rostyslav

    Biomolecules

    2024  Volume 14, Issue 2

    Abstract: Pulmonary fibrosis, severe alveolitis, and the inability to restore alveolar epithelial architecture are primary causes of respiratory failure in fatal COVID-19 cases. However, the factors contributing to abnormal fibrosis in critically ill COVID-19 ... ...

    Abstract Pulmonary fibrosis, severe alveolitis, and the inability to restore alveolar epithelial architecture are primary causes of respiratory failure in fatal COVID-19 cases. However, the factors contributing to abnormal fibrosis in critically ill COVID-19 patients remain unclear. This study analyzed the histopathology of lung specimens from eight COVID-19 and six non-COVID-19 postmortems. We assessed the distribution and changes in extracellular matrix (ECM) proteins, including elastin and collagen, in lung alveoli through morphometric analyses. Our findings reveal the significant degradation of elastin fibers along the thin alveolar walls of the lung parenchyma, a process that precedes the onset of interstitial collagen deposition and widespread intra-alveolar fibrosis. Lungs with collapsed alveoli and organized fibrotic regions showed extensive fragmentation of elastin fibers, accompanied by alveolar epithelial cell death. Immunoblotting of lung autopsy tissue extracts confirmed elastin degradation. Importantly, we found that the loss of elastin was strongly correlated with the induction of neutrophil elastase (NE), a potent protease that degrades ECM. This study affirms the critical role of neutrophils and neutrophil enzymes in the pathogenesis of COVID-19. Consistently, we observed increased staining for peptidyl arginine deiminase, a marker for neutrophil extracellular trap release, and myeloperoxidase, an enzyme-generating reactive oxygen radical, indicating active neutrophil involvement in lung pathology. These findings place neutrophils and elastin degradation at the center of impaired alveolar function and argue that elastolysis and alveolitis trigger abnormal ECM repair and fibrosis in fatal COVID-19 cases. Importantly, this study has implications for severe COVID-19 complications, including long COVID and other chronic inflammatory and fibrotic disorders.
    MeSH term(s) Humans ; Neutrophils/metabolism ; Post-Acute COVID-19 Syndrome ; COVID-19/metabolism ; Lung/metabolism ; Elastin ; Collagen/metabolism ; Extracellular Matrix Proteins/metabolism ; Endopeptidases ; Extracellular Matrix/metabolism ; Fibrosis
    Chemical Substances Elastin (9007-58-3) ; Collagen (9007-34-5) ; Extracellular Matrix Proteins ; Endopeptidases (EC 3.4.-)
    Language English
    Publishing date 2024-02-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom14020236
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: Identification of two major QTLs for pod shell thickness in peanut (Arachis hypogaea L.) using BSA-seq analysis.

    Liu, Hongfei / Zheng, Zheng / Sun, Ziqi / Qi, Feiyan / Wang, Juan / Wang, Mengmeng / Dong, Wenzhao / Cui, Kailu / Zhao, Mingbo / Wang, Xiao / Zhang, Meng / Wu, Xiaohui / Wu, Yue / Luo, Dandan / Huang, Bingyan / Zhang, Zhongxin / Cao, Gangqiang / Zhang, Xinyou

    BMC genomics

    2024  Volume 25, Issue 1, Page(s) 65

    Abstract: Background: Pod shell thickness (PST) is an important agronomic trait of peanut because it affects the ability of shells to resist pest infestations and pathogen attacks, while also influencing the peanut shelling process. However, very few studies have ...

    Abstract Background: Pod shell thickness (PST) is an important agronomic trait of peanut because it affects the ability of shells to resist pest infestations and pathogen attacks, while also influencing the peanut shelling process. However, very few studies have explored the genetic basis of PST.
    Results: An F
    Conclusions: The QTLs identified and molecular markers developed in this study may lay the foundation for breeding cultivars with a shell thickness suitable for mechanized peanut shelling.
    MeSH term(s) Quantitative Trait Loci ; Arachis/genetics ; Chromosome Mapping ; Plant Breeding ; Phenotype
    Language English
    Publishing date 2024-01-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041499-7
    ISSN 1471-2164 ; 1471-2164
    ISSN (online) 1471-2164
    ISSN 1471-2164
    DOI 10.1186/s12864-024-10005-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article: Mining the Antibody Repertoire for Solutions to SARS-CoV-2

    Meng, Wenzhao / Rosenfeld, Aaron M / Luning Prak, Eline T

    Cell Host Microbe

    Abstract: In this issue of Cell Host & Microbe, Nielsen and colleagues sequence antibody repertoires of patients with severe COVID-19 to reveal potentially convergent features on the background of a larger, polyclonal response. Their findings suggest that, as ... ...

    Abstract In this issue of Cell Host & Microbe, Nielsen and colleagues sequence antibody repertoires of patients with severe COVID-19 to reveal potentially convergent features on the background of a larger, polyclonal response. Their findings suggest that, as databases improve, it may be possible to monitor virus-specific B cells after infection or vaccination using antibody sequencing.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #838112
    Database COVID19

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  9. Article ; Online: Application of informatics in cancer research and clinical practice: Opportunities and challenges.

    Hong, Na / Sun, Gang / Zuo, Xiuran / Chen, Meng / Liu, Li / Wang, Jiani / Feng, Xiaobin / Shi, Wenzhao / Gong, Mengchun / Ma, Pengcheng

    Cancer innovation

    2022  Volume 1, Issue 1, Page(s) 80–91

    Abstract: Cancer informatics has significantly progressed in the big data era. We summarize the application of informatics approaches to the cancer domain from both the informatics perspective (e.g., data management and data science) and the clinical perspective ( ... ...

    Abstract Cancer informatics has significantly progressed in the big data era. We summarize the application of informatics approaches to the cancer domain from both the informatics perspective (e.g., data management and data science) and the clinical perspective (e.g., cancer screening, risk assessment, diagnosis, treatment, and prognosis). We discuss various informatics methods and tools that are widely applied in cancer research and practices, such as cancer databases, data standards, terminologies, high-throughput omics data mining, machine-learning algorithms, artificial intelligence imaging, and intelligent radiation. We also address the informatics challenges within the cancer field that pursue better treatment decisions and patient outcomes, and focus on how informatics can provide opportunities for cancer research and practices. Finally, we conclude that the interdisciplinary nature of cancer informatics and collaborations are major drivers for future research and applications in clinical practices. It is hoped that this review is instrumental for cancer researchers and clinicians with its informatics-specific insights.
    Language English
    Publishing date 2022-06-15
    Publishing country England
    Document type Journal Article ; Review
    ISSN 2770-9183
    ISSN (online) 2770-9183
    DOI 10.1002/cai2.9
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  10. Article ; Online: Bulk gDNA Sequencing of Antibody Heavy-Chain Gene Rearrangements for Detection and Analysis of B-Cell Clone Distribution: A Method by the AIRR Community.

    Rosenfeld, Aaron M / Meng, Wenzhao / Horne, Kalisse I / Chen, Elaine C / Bagnara, Davide / Stervbo, Ulrik / Luning Prak, Eline T

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2453, Page(s) 317–343

    Abstract: In this method we illustrate how to amplify, sequence, and analyze antibody/immunoglobulin (IG) heavy-chain gene rearrangements from genomic DNA that is derived from bulk populations of cells by next-generation sequencing (NGS). We focus on human source ... ...

    Abstract In this method we illustrate how to amplify, sequence, and analyze antibody/immunoglobulin (IG) heavy-chain gene rearrangements from genomic DNA that is derived from bulk populations of cells by next-generation sequencing (NGS). We focus on human source material and illustrate how bulk gDNA-based sequencing can be used to examine clonal architecture and networks in different samples that are sequenced from the same individual. Although bulk gDNA-based sequencing can be performed on both IG heavy (IGH) or kappa/lambda light (IGK/IGL) chains, we focus here on IGH gene rearrangements because IG heavy chains are more diverse, tend to harbor higher levels of somatic hypermutations (SHM), and are more reliable for clone identification and tracking. We also provide a procedure, including code, and detailed instructions for processing and annotation of the NGS data. From these data we show how to identify expanded clones, visualize the overall clonal landscape, and track clonal lineages in different samples from the same individual. This method has a broad range of applications, including the identification and monitoring of expanded clones, the analysis of blood and tissue-based clonal networks, and the study of immune responses including clonal evolution.
    MeSH term(s) B-Lymphocytes ; Clone Cells ; DNA ; Gene Rearrangement ; Humans ; Immunoglobulin Heavy Chains/genetics
    Chemical Substances Immunoglobulin Heavy Chains ; DNA (9007-49-2)
    Language English
    Publishing date 2022-05-24
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2115-8_18
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top