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  1. Book: Expressed protein ligation

    Vila-Perelló, Miquel

    methods and protocols

    (Methods in molecular biology ; 2133)

    2020  

    Author's details edited by Miguel Vila-Perelló
    Series title Methods in molecular biology ; 2133
    Collection
    Language English
    Size x, 362 Seiten, Illustrationen, Diagramme
    Publisher Humana Press
    Publishing place New York, NY
    Publishing country United States
    Document type Book
    HBZ-ID HT020403545
    ISBN 978-1-07-160433-5 ; 9781071604342 ; 1-07-160433-3 ; 1071604341
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    Zs A 7042 -2133- verfügbar in Königswinter Königswinter

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  2. Article ; Online: Neuromelanin, aging, and neuronal vulnerability in Parkinson's disease.

    Vila, Miquel

    Movement disorders : official journal of the Movement Disorder Society

    2019  Volume 34, Issue 10, Page(s) 1440–1451

    Abstract: Neuromelanin, a dark brown intracellular pigment, has long been associated with Parkinson's disease (PD). In PD, neuromelanin-containing neurons preferentially degenerate, tell-tale neuropathological inclusions form in close association with this pigment, ...

    Abstract Neuromelanin, a dark brown intracellular pigment, has long been associated with Parkinson's disease (PD). In PD, neuromelanin-containing neurons preferentially degenerate, tell-tale neuropathological inclusions form in close association with this pigment, and neuroinflammation is restricted to neuromelanin-containing areas. In humans, neuromelanin accumulates with age, which in turn is the main risk factor for PD. The potential contribution of neuromelanin to PD pathogenesis remains unknown because, in contrast to humans, common laboratory animals lack neuromelanin. The recent introduction of a rodent model exhibiting an age-dependent production of human-like neuromelanin has allowed, for the first time, for the consequences of progressive neuromelanin accumulation-up to levels reached in elderly human brains-to be assessed in vivo. In these animals, intracellular neuromelanin accumulation above a specific threshold compromises neuronal function and triggers a PD-like pathology. As neuromelanin levels reach this threshold in PD patients and presymptomatic PD patients, the modulation of neuromelanin accumulation could provide a therapeutic benefit for PD patients and delay brain aging. © 2019 The Author. Movement Disorders published by Wiley Periodicals, Inc. on behalf of International Parkinson and Movement Disorder Society.
    MeSH term(s) Aging/physiology ; Brain/drug effects ; Brain/pathology ; Humans ; Melanins/pharmacology ; Neurons/drug effects ; Neurons/pathology ; Parkinson Disease/diagnostic imaging ; Parkinson Disease/drug therapy ; Parkinson Disease/pathology ; Parkinsonian Disorders/drug therapy
    Chemical Substances Melanins ; neuromelanin
    Language English
    Publishing date 2019-06-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 607633-6
    ISSN 1531-8257 ; 0885-3185
    ISSN (online) 1531-8257
    ISSN 0885-3185
    DOI 10.1002/mds.27776
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.MO 238: Show issues

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  3. Article ; Online: Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea.

    Vila-Farré, Miquel / Vu, Hanh Thi-Kim / Rink, Jochen C

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2680, Page(s) 107–119

    Abstract: Whole-mount in situ hybridization (WISH), colorimetric or fluorescent (FISH), allows for the visualization of endogenous RNA. For planarians, robust WISH protocols exist for small-sized animals (>5 mm) of the model species Schmidtea mediterranea and ... ...

    Abstract Whole-mount in situ hybridization (WISH), colorimetric or fluorescent (FISH), allows for the visualization of endogenous RNA. For planarians, robust WISH protocols exist for small-sized animals (>5 mm) of the model species Schmidtea mediterranea and Dugesia japonica. However, the sexual strain of Schmidtea mediterranea studied for germline development and function reaches much larger body sizes in excess of 2 cm. The existing whole-mount WISH protocols are not optimal for such large specimens, owing to insufficient tissue permeabilization. Here, we describe a robust WISH protocol for 12-16 mm long sexually mature Schmidtea mediterranea individuals that could serve as a starting point for adapting WISH to other large planarian species.
    MeSH term(s) Animals ; Mediterranea ; Planarians/genetics ; RNA ; Germ Cells ; In Situ Hybridization
    Chemical Substances RNA (63231-63-0)
    Language English
    Publishing date 2023-07-10
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3275-8_7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Rh(I) Complexes with Hemilabile Thioether-Functionalized NHC Ligands as Catalysts for [2 + 2 + 2] Cycloaddition of 1,5-Bisallenes and Alkynes.

    Vila, Jordi / Solà, Miquel / Achard, Thierry / Bellemin-Laponnaz, Stéphane / Pla-Quintana, Anna / Roglans, Anna

    ACS catalysis

    2023  Volume 13, Issue 5, Page(s) 3201–3210

    Abstract: The [2 + 2 + 2] cycloaddition of 1,5-bisallenes and alkynes under the catalysis of Rh(I) with hemilabile thioether-functionalized N-heterocyclic carbene ligands is described. This protocol effectively provides an entry to ... ...

    Abstract The [2 + 2 + 2] cycloaddition of 1,5-bisallenes and alkynes under the catalysis of Rh(I) with hemilabile thioether-functionalized N-heterocyclic carbene ligands is described. This protocol effectively provides an entry to different
    Language English
    Publishing date 2023-02-17
    Publishing country United States
    Document type Journal Article
    ISSN 2155-5435
    ISSN 2155-5435
    DOI 10.1021/acscatal.2c05790
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Bacterial Adaptive Memory in Methicillin-Resistant

    Fernández-Barat, Laia / López-Aladid, Ruben / Vázquez, Nil / Cabrera, Roberto / Vila, Jordi / Ferrer, Miquel / Torres, Antoni

    Pathogens (Basel, Switzerland)

    2024  Volume 13, Issue 2

    Abstract: Objectives: To evaluate the expression dynamics of biofilm genes in methicillin-resistant : Methods: Biofilm was grown (24 h) in tryptic broth soy plus 0.25% glucose for a clinical MRSA isolate in planktonic state and after sessile growth named ETT- ... ...

    Abstract Objectives: To evaluate the expression dynamics of biofilm genes in methicillin-resistant
    Methods: Biofilm was grown (24 h) in tryptic broth soy plus 0.25% glucose for a clinical MRSA isolate in planktonic state and after sessile growth named ETT-MRSA (S2, S3, S4, S5, S6, S7). Gene expression of five biofilm-related genes (
    Results: The MRSA isolates retrieved from ETT were capable of producing biofilms dependent on
    Conclusions: MRSA loses its biofilm gene expression in vitro, by adaptive features across multiple generations, as evidenced by the progressive downregulation of
    Language English
    Publishing date 2024-02-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens13020144
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Parkinson disease, substantia nigra vulnerability, and calbindin expression: Enlightening the darkness?

    Blesa, Javier / Vila, Miquel

    Movement disorders : official journal of the Movement Disorder Society

    2019  Volume 34, Issue 2, Page(s) 161–163

    MeSH term(s) Calbindins ; Darkness ; Dopaminergic Neurons ; Humans ; MPTP Poisoning ; Parkinson Disease ; Substantia Nigra
    Chemical Substances Calbindins
    Language English
    Publishing date 2019-01-24
    Publishing country United States
    Document type Editorial ; Comment
    ZDB-ID 607633-6
    ISSN 1531-8257 ; 0885-3185
    ISSN (online) 1531-8257
    ISSN 0885-3185
    DOI 10.1002/mds.27618
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Quantum-classical approach to the reaction dynamics in a superfluid helium nanodroplet. The Ne

    Blancafort-Jorquera, Miquel / Vilà, Arnau / González, Miguel

    Physical chemistry chemical physics : PCCP

    2019  Volume 21, Issue 43, Page(s) 24218–24231

    Abstract: The dynamics of the ... ...

    Abstract The dynamics of the Ne
    Language English
    Publishing date 2019-10-29
    Publishing country England
    Document type Journal Article
    ZDB-ID 1476244-4
    ISSN 1463-9084 ; 1463-9076
    ISSN (online) 1463-9084
    ISSN 1463-9076
    DOI 10.1039/c9cp04561a
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Highly Selective Synthesis of Seven-Membered Azaspiro Compounds by a Rh(I)-Catalyzed Cycloisomerization/Diels-Alder Cascade of 1,5-Bisallenes.

    Vila, Jordi / Solà, Miquel / Pla-Quintana, Anna / Roglans, Anna

    The Journal of organic chemistry

    2022  Volume 87, Issue 8, Page(s) 5279–5286

    Abstract: The synthesis of spiro compounds featuring seven- and six-membered rings in the spirobicyclic motif is successfully achieved through a cascade process encompassing a rhodium(I)-catalyzed cycloisomerization followed by a highly selective Diels-Alder ... ...

    Abstract The synthesis of spiro compounds featuring seven- and six-membered rings in the spirobicyclic motif is successfully achieved through a cascade process encompassing a rhodium(I)-catalyzed cycloisomerization followed by a highly selective Diels-Alder homodimerization. The scope of the reaction is analyzed based on a series of synthetic substrates, and control experiments and DFT calculations led us to justify the exquisite degree of selectivity observed.
    MeSH term(s) Catalysis ; Density Functional Theory ; Rhodium/chemistry
    Chemical Substances Rhodium (DMK383DSAC)
    Language English
    Publishing date 2022-03-24
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 123490-0
    ISSN 1520-6904 ; 0022-3263
    ISSN (online) 1520-6904
    ISSN 0022-3263
    DOI 10.1021/acs.joc.2c00065
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Relevance of tissue-resident memory CD8 T cells in the onset of Parkinson's disease and examination of its possible etiologies: infectious or autoimmune?

    de Fàbregues, Oriol / Sellés, Maria / Ramos-Vicente, David / Roch, Gerard / Vila, Miquel / Bové, Jordi

    Neurobiology of disease

    2023  Volume 187, Page(s) 106308

    Abstract: Tissue-resident memory CD8 T cells are responsible for local immune surveillance in different tissues, including the brain. They constitute the first line of defense against pathogens and cancer cells and play a role in autoimmunity. A recently published ...

    Abstract Tissue-resident memory CD8 T cells are responsible for local immune surveillance in different tissues, including the brain. They constitute the first line of defense against pathogens and cancer cells and play a role in autoimmunity. A recently published study demonstrated that CD8 T cells with markers of residency containing distinct granzymes and interferon-γ infiltrate the parenchyma of the substantia nigra and contact dopaminergic neurons in an early premotor stage of Parkinson's disease. This infiltration precedes α-synuclein aggregation and neuronal loss in the substantia nigra, suggesting a relevant role for CD8 T cells in the onset of the disease. To date, the nature of the antigen that initiates the adaptive immune response remains unknown. This review will discuss the role of tissue-resident memory CD8 T cells in brain immune homeostasis and in the onset of Parkinson's disease and other neurological diseases. We also discuss how aging and genetic factors can affect the CD8 T cell immune response and how animal models can be misleading when studying human-related immune response. Finally, we speculate about a possible infectious or autoimmune origin of Parkinson's disease.
    Language English
    Publishing date 2023-09-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1211786-9
    ISSN 1095-953X ; 0969-9961
    ISSN (online) 1095-953X
    ISSN 0969-9961
    DOI 10.1016/j.nbd.2023.106308
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Personal exposure to radiofrequency electromagnetic fields in various occupations in Spain and France.

    Turuban, Maxime / Kromhout, Hans / Vila, Javier / Vallbona-Vistós, Miquel / Baldi, Isabelle / Turner, Michelle C

    Environment international

    2023  Volume 180, Page(s) 108156

    Abstract: Background: A preliminary job-exposure matrix (JEM) for radiofrequency electromagnetic fields (RF-EMF) was created based on self-reported occupational information from a multi-country population-based study of approximately 10,000 participants combined ... ...

    Abstract Background: A preliminary job-exposure matrix (JEM) for radiofrequency electromagnetic fields (RF-EMF) was created based on self-reported occupational information from a multi-country population-based study of approximately 10,000 participants combined with available measurement data compiled in a source-exposure matrix (spot measurements). In order to address the limited personal occupational RF-EMF measurement data available in the literature, we performed a measurement campaign among workers in various occupations in Spain and France.
    Methods: Personal full-shift measurements were conducted using RadMan 2XT™ (Narda) devices. A worker diary was used to capture information on occupational and background sources of RF exposure during the shift. Inclusion of occupations to be measured was initially based on exposure prevalence and level information in the preliminary JEM and expert judgment.
    Results: Personal full-shift measurements were conducted among 333 workers representing 46 ISCO88 occupations. Exposure to electric (E) and magnetic (H) fields was infrequent with >99% of measurements below the detection limit of the device (≥1% of the 1998 ICNIRP standards). A total of 50.2% and 77.2% of workers were ever exposed to E and H fields respectively (having at least one recorded 1-second measurement above the detection limit). Workers in elementary occupations, technicians and associate professionals, plant and machine operators and assemblers had somewhat greater numbers of measurements above the detection limit, higher maximum values and longer exposure durations. A small proportion of measurements were ≥100% of the standards, though these exceedances were brief (generally a few seconds in duration). Female workers and workers reporting use of any RF-EMF emitting source were more likely to have a measured exposure to E and H fields.
    Conclusion: We conducted personal RF-EMF measurements among workers in various occupations in Spain and France. Overall, RF-EMF exposure ≥1 % ICNIRP was infrequent, despite some intermittent exposures ≥100% observed among workers in some occupations.
    Language English
    Publishing date 2023-08-18
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 554791-x
    ISSN 1873-6750 ; 0160-4120
    ISSN (online) 1873-6750
    ISSN 0160-4120
    DOI 10.1016/j.envint.2023.108156
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