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Article: Development of a Genome-Informed Protocol for Detection of

Díaz, Daniela / Zamorano, Alan / García, Héctor / Ramos, Cecilia / Cui, Weier / Carreras, Claudia / Beltrán, María Francisca / Sagredo, Boris / Pinto, Manuel / Fiore, Nicola

Plants (Basel, Switzerland)

2023 Volume 12, Issue 24

Abstract: One of the causal agents of bacterial canker ... ...

Abstract	One of the causal agents of bacterial canker is
Language	English
Publishing date	2023-12-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants12244119
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Article ; Online: CartoCell, a high-content pipeline for 3D image analysis, unveils cell morphology patterns in epithelia.

Andrés-San Román, Jesús A / Gordillo-Vázquez, Carmen / Franco-Barranco, Daniel / Morato, Laura / Fernández-Espartero, Cecilia H / Baonza, Gabriel / Tagua, Antonio / Vicente-Munuera, Pablo / Palacios, Ana M / Gavilán, María P / Martín-Belmonte, Fernando / Annese, Valentina / Gómez-Gálvez, Pedro / Arganda-Carreras, Ignacio / Escudero, Luis M

Cell reports methods

2023 Volume 3, Issue 10, Page(s) 100597

Abstract: Decades of research have not yet fully explained the mechanisms of epithelial self-organization and 3D packing. Single-cell analysis of large 3D epithelial libraries is crucial for understanding the assembly and function of whole tissues. Combining 3D ... ...

Abstract	Decades of research have not yet fully explained the mechanisms of epithelial self-organization and 3D packing. Single-cell analysis of large 3D epithelial libraries is crucial for understanding the assembly and function of whole tissues. Combining 3D epithelial imaging with advanced deep-learning segmentation methods is essential for enabling this high-content analysis. We introduce CartoCell, a deep-learning-based pipeline that uses small datasets to generate accurate labels for hundreds of whole 3D epithelial cysts. Our method detects the realistic morphology of epithelial cells and their contacts in the 3D structure of the tissue. CartoCell enables the quantification of geometric and packing features at the cellular level. Our single-cell cartography approach then maps the distribution of these features on 2D plots and 3D surface maps, revealing cell morphology patterns in epithelial cysts. Additionally, we show that CartoCell can be adapted to other types of epithelial tissues.
MeSH term(s)	Humans ; Imaging, Three-Dimensional/methods ; Image Processing, Computer-Assisted/methods ; Epithelium ; Epithelial Cells ; Cysts
Language	English
Publishing date	2023-09-25
Publishing country	United States
Document type	Journal Article
ISSN	2667-2375
ISSN (online)	2667-2375
DOI	10.1016/j.crmeth.2023.100597
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Article ; Online: Selection process and causes of non-eligibility for CD19 CAR-T cell therapy in patients with relapsed/refractory aggressive B-cell non-Hodgkin lymphoma in a European center.

Carpio, Cecilia / Iacoboni, Gloria / Villacampa, Guillermo / Catalá, Eva / Bobillo, Sabela / Pérez, Ana / Jiménez, Moraima / Segura, Laura / Olivé, Mireia / Farriols, Anna / Abrisqueta, Pau / Valcárcel, David / Carreras, María Josep / Bosch, Francesc / Barba, Pere

Leukemia & lymphoma

2021 Volume 62, Issue 9, Page(s) 2288–2291

MeSH term(s)	Antigens, CD19 ; B-Lymphocytes ; Cell- and Tissue-Based Therapy ; Humans ; Immunotherapy, Adoptive ; Lymphoma, B-Cell/therapy ; Receptors, Antigen, T-Cell ; Receptors, Chimeric Antigen/genetics
Chemical Substances	Antigens, CD19 ; Receptors, Antigen, T-Cell ; Receptors, Chimeric Antigen
Language	English
Publishing date	2021-04-24
Publishing country	United States
Document type	Letter
ZDB-ID	1042374-6
ISSN	1029-2403 ; 1042-8194
ISSN (online)	1029-2403
ISSN	1042-8194
DOI	10.1080/10428194.2021.1913141
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Article ; Online: p66

Pérez, Hernán / Finocchietto, Paola Vanesa / Alippe, Yael / Rebagliati, Inés / Elguero, María Eugenia / Villalba, Nerina / Poderoso, Juan José / Carreras, María Cecilia

Oxidative medicine and cellular longevity

2018 Volume 2018, Page(s) 8561892

Abstract: Programmed and damage aging theories have traditionally been conceived as stand-alone schools of thought. However, the ... ...

Abstract	Programmed and damage aging theories have traditionally been conceived as stand-alone schools of thought. However, the p66
MeSH term(s)	Aging/metabolism ; Animals ; Brain/metabolism ; Homeostasis ; Mice ; Mice, Knockout ; Mitochondria/metabolism ; Reactive Nitrogen Species/metabolism ; Sirtuin 3/metabolism ; Src Homology 2 Domain-Containing, Transforming Protein 1/metabolism
Chemical Substances	Reactive Nitrogen Species ; Shc1 protein, mouse ; Sirt3 protein, mouse ; Src Homology 2 Domain-Containing, Transforming Protein 1 ; Sirtuin 3 (EC 3.5.1.-)
Language	English
Publishing date	2018-03-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	2455981-7
ISSN	1942-0994 ; 1942-0994
ISSN (online)	1942-0994
ISSN	1942-0994
DOI	10.1155/2018/8561892
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Article ; Online: Arsenic exposure of child populations in Northern Argentina.

Calatayud, Marta / Farias, Silvia Sara / de Paredes, Gladys Soria / Olivera, Mónica / Carreras, Natalia Ávila / Giménez, Maria Cecilia / Devesa, Vicenta / Vélez, Dinoraz

The Science of the total environment

2019 Volume 669, Page(s) 1–6

Abstract: Chronic exposure to inorganic arsenic (As) is associated with numerous adverse effects. Argentina is one of the countries affected by arsenicism; however, there are few studies that evaluate inorganic As exposure and its effects on child population. The ... ...

Abstract	Chronic exposure to inorganic arsenic (As) is associated with numerous adverse effects. Argentina is one of the countries affected by arsenicism; however, there are few studies that evaluate inorganic As exposure and its effects on child population. The aim of this study is to evaluate exposure to As through water and food in child populations living in the provinces of Santiago del Estero and Chaco (n = 101), and to determine the impact of this exposure analysing biomarkers of exposure (urine and hair As contents) and effect [8-oxo-7,8-dihydro-2'-deoxyguanosine (8-OHdG)]. The populations selected live in three areas with different levels of As in the drinking water (Santa Teresa de Carballo, 0.925 mg/L; Taco Pozo, 0.210 mg/L; Jumi Pozo, 0.016 mg/L). The As intakes through water and food are especially high in the areas with the greatest As exposure (Santa Teresa de Carballo, 1575 ± 8 μg/day; Taco Pozo, 386 ± 8 μg/day; Jumi Pozo, 39 ± 1 μg/day). The total As contents in most of the samples of hair (0.11-13.11 mg/kg) and urine (31-4258 μg/g creatinine) are higher than the reference values (hair: 1 mg/kg; urine: 50 μg/g creatinine). The increase in the level of As exposure alters the profile of metabolites in urine, with a decrease of dimethylarsinic acid (10%) and an increase in the percentages of monomethylarsonic acid (4%) and inorganic As (6%). The results also show high values of 8-OHdG (3.7-37.8 μg/g creatinine), a oxidative DNA damage marker, in the two areas with greater As exposure.
MeSH term(s)	Argentina ; Arsenic/metabolism ; Arsenicals/metabolism ; Child, Preschool ; Deoxyguanosine/analogs & derivatives ; Deoxyguanosine/metabolism ; Diet ; Drinking Water ; Environmental Exposure ; Environmental Monitoring ; Environmental Pollutants/metabolism ; Female ; Hair/chemistry ; Humans ; Male ; Students
Chemical Substances	Arsenicals ; Drinking Water ; Environmental Pollutants ; 8-oxo-7-hydrodeoxyguanosine (88847-89-6) ; Deoxyguanosine (G9481N71RO) ; Arsenic (N712M78A8G)
Language	English
Publishing date	2019-02-27
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2019.02.415
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Zs.A 949: Show issues

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Article: Mitochondrial nitric oxide in the signaling of cell integrated responses.

Carreras, Maria Cecilia / Poderoso, Juan José

American journal of physiology. Cell physiology

2007 Volume 292, Issue 5, Page(s) C1569–80

Abstract: Mitochondria are the specialized organelles for energy metabolism, but, as a typical example of system biology, they also activate a multiplicity of pathways that modulate cell proliferation and mitochondrial biogenesis or oppositely promote cell arrest ... ...

Abstract	Mitochondria are the specialized organelles for energy metabolism, but, as a typical example of system biology, they also activate a multiplicity of pathways that modulate cell proliferation and mitochondrial biogenesis or oppositely promote cell arrest and programmed cell death by a limited number of oxidative or nitrosative reactions. These reactions are influenced by matrix nitric oxide (NO) steady-state concentration, either from local production or by gas diffusion to mitochondria from the canonical sources. Likewise, in a range of approximately 30-200 nM, NO turns mitochondrial O(2) utilization down by binding to cytochrome oxidase and elicits a burst of superoxide anion and hydrogen peroxide that diffuses outside mitochondria. Depending on NO levels and antioxidant defenses, more or less H(2)O(2) accumulates in cytosol and nucleus, and the resulting redox grading contributes to dual activation of proliferating and proapoptotic cascades, like ERK1/2 or p38 MAPK. Moreover, these sequential activating pathways participate in rat liver and brain development and in thyroid modulation of mitochondrial metabolism and contribute to hypothyroid phenotype through complex I nitration. On the contrary, lack of NO disrupts pathways like S-nitrosylation or H(2)O(2) production and likewise is a gateway to disease in amyotrophic lateral sclerosis with superoxide dismutase 1 mutations or to cancer proliferation.
MeSH term(s)	Animals ; Apoptosis ; Brain/growth & development ; Brain/metabolism ; Cell Cycle/physiology ; Cell Proliferation ; Humans ; Hydrogen Peroxide/metabolism ; Hypothyroidism/metabolism ; Hypothyroidism/physiopathology ; Liver/growth & development ; Liver/metabolism ; Mitochondria/metabolism ; Mitogen-Activated Protein Kinases/metabolism ; Models, Biological ; Nitric Oxide/metabolism ; Nitric Oxide Synthase/metabolism ; Oxidation-Reduction ; Oxidative Stress ; Peroxynitrous Acid/metabolism ; Reactive Oxygen Species/metabolism ; Signal Transduction
Chemical Substances	Reactive Oxygen Species ; Peroxynitrous Acid (14691-52-2) ; Nitric Oxide (31C4KY9ESH) ; Hydrogen Peroxide (BBX060AN9V) ; Nitric Oxide Synthase (EC 1.14.13.39) ; Mitogen-Activated Protein Kinases (EC 2.7.11.24)
Language	English
Publishing date	2007-05-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	392098-7
ISSN	1522-1563 ; 0363-6143
ISSN (online)	1522-1563
ISSN	0363-6143
DOI	10.1152/ajpcell.00248.2006
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Article: Arsenic exposure of child populations in Northern Argentina

Calatayud, Marta / Silvia Sara Farias / Gladys Soria de Paredes / Mónica Olivera / Natalia Ávila Carreras / Maria Cecilia Giménez / Vicenta Devesa / Dinoraz Vélez

Science of the total environment. 2019 June 15, v. 669

2019

Abstract	Chronic exposure to inorganic arsenic (As) is associated with numerous adverse effects. Argentina is one of the countries affected by arsenicism; however, there are few studies that evaluate inorganic As exposure and its effects on child population. The aim of this study is to evaluate exposure to As through water and food in child populations living in the provinces of Santiago del Estero and Chaco (n = 101), and to determine the impact of this exposure analysing biomarkers of exposure (urine and hair As contents) and effect [8-oxo-7,8-dihydro-2′-deoxyguanosine (8-OHdG)]. The populations selected live in three areas with different levels of As in the drinking water (Santa Teresa de Carballo, 0.925 mg/L; Taco Pozo, 0.210 mg/L; Jumi Pozo, 0.016 mg/L). The As intakes through water and food are especially high in the areas with the greatest As exposure (Santa Teresa de Carballo, 1575 ± 8 μg/day; Taco Pozo, 386 ± 8 μg/day; Jumi Pozo, 39 ± 1 μg/day).The total As contents in most of the samples of hair (0.11–13.11 mg/kg) and urine (31–4258 μg/g creatinine) are higher than the reference values (hair: 1 mg/kg; urine: 50 μg/g creatinine). The increase in the level of As exposure alters the profile of metabolites in urine, with a decrease of dimethylarsinic acid (10%) and an increase in the percentages of monomethylarsonic acid (4%) and inorganic As (6%). The results also show high values of 8-OHdG (3.7–37.8 μg/g creatinine), a oxidative DNA damage marker, in the two areas with greater As exposure.
Keywords	DNA damage ; adverse effects ; arsenic ; biomarkers ; cacodylic acid ; children ; chronic exposure ; creatinine ; drinking water ; metabolites ; normal values ; urine ; Argentina
Language	English
Dates of publication	2019-0615
Size	p. 1-6.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2019.02.415
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Mitochondrial regulation of cell cycle and proliferation.

Antico Arciuch, Valeria Gabriela / Elguero, María Eugenia / Poderoso, Juan José / Carreras, María Cecilia

Antioxidants & redox signaling

2012 Volume 16, Issue 10, Page(s) 1150–1180

Abstract: Eukaryotic mitochondria resulted from symbiotic incorporation of α-proteobacteria into ancient archaea species. During evolution, mitochondria lost most of the prokaryotic bacterial genes and only conserved a small fraction including those encoding 13 ... ...

Abstract	Eukaryotic mitochondria resulted from symbiotic incorporation of α-proteobacteria into ancient archaea species. During evolution, mitochondria lost most of the prokaryotic bacterial genes and only conserved a small fraction including those encoding 13 proteins of the respiratory chain. In this process, many functions were transferred to the host cells, but mitochondria gained a central role in the regulation of cell proliferation and apoptosis, and in the modulation of metabolism; accordingly, defective organelles contribute to cell transformation and cancer, diabetes, and neurodegenerative diseases. Most cell and transcriptional effects of mitochondria depend on the modulation of respiratory rate and on the production of hydrogen peroxide released into the cytosol. The mitochondrial oxidative rate has to remain depressed for cell proliferation; even in the presence of O₂, energy is preferentially obtained from increased glycolysis (Warburg effect). In response to stress signals, traffic of pro- and antiapoptotic mitochondrial proteins in the intermembrane space (B-cell lymphoma-extra large, Bcl-2-associated death promoter, Bcl-2 associated X-protein and cytochrome c) is modulated by the redox condition determined by mitochondrial O₂ utilization and mitochondrial nitric oxide metabolism. In this article, we highlight the traffic of the different canonical signaling pathways to mitochondria and the contributions of organelles to redox regulation of kinases. Finally, we analyze the dynamics of the mitochondrial population in cell cycle and apoptosis.
MeSH term(s)	Animals ; Cell Cycle ; Cell Death ; Cell Proliferation ; Humans ; Mitochondria/physiology ; Oxidation-Reduction ; Phosphotransferases/metabolism ; Reactive Oxygen Species/metabolism ; Signal Transduction
Chemical Substances	Reactive Oxygen Species ; Phosphotransferases (EC 2.7.-)
Language	English
Publishing date	2012-01-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1483836-9
ISSN	1557-7716 ; 1523-0864
ISSN (online)	1557-7716
ISSN	1523-0864
DOI	10.1089/ars.2011.4085
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Zs.A 5488: Show issues

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Article ; Online: Progesterone prevents mitochondrial dysfunction in the spinal cord of wobbler mice.

Gonzalez Deniselle, Maria Claudia / Carreras, Maria Cecilia / Garay, Laura / Gargiulo-Monachelli, Gisella / Meyer, Maria / Poderoso, Juan Jose / De Nicola, Alejandro F

Journal of neurochemistry

2012 Volume 122, Issue 1, Page(s) 185–195

Abstract: In the Wobbler mouse, a mutation of the Vps54 protein increases oxidative stress in spinal motoneurons, associated to toxic levels of nitric oxide and hyperactivity of nitric oxide synthase (NOS). Progesterone neuroprotection has been reported for ... ...

Abstract	In the Wobbler mouse, a mutation of the Vps54 protein increases oxidative stress in spinal motoneurons, associated to toxic levels of nitric oxide and hyperactivity of nitric oxide synthase (NOS). Progesterone neuroprotection has been reported for several CNS diseases, including the Wobbler mouse neurodegeneration. In the present study, we analyzed progesterone effects on mitochondrial-associated parameters of symptomatic Wobbler mice. The activities of mitochondrial respiratory chain complexes I, II-III and IV and protein levels of mitochondrial and cytosolic NOS were determined in cervical and lumbar cords from control, Wobbler and Wobbler mice receiving a progesterone implant for 18 days. We found a significant reduction of complex I and II-III activities in mitochondria and increased protein levels of mitochondrial, but not cytosolic nNOS, in the cervical cord of Wobbler mice. Progesterone treatment prevented the reduction of complex I in the cervical region and the increased level of mitochondrial nNOS. Wobbler motoneurons also showed accumulation of amyloid precursor protein immunoreactivity and decreased activity and immunostaining of MnSOD. Progesterone treatment avoided these abnormalities. Therefore, administration of progesterone to clinically afflicted Wobblers (i) prevented the abnormal increase of mitochondrial nNOS and normalized respiratory complex I; (ii) decreased amyloid precursor protein accumulation, a sign of axonal degeneration, and (iii) increased superoxide dismutation. Thus, progesterone neuroprotection decreases mitochondriopathy of Wobbler mouse cervical spinal cord.
MeSH term(s)	Albinism, Oculocutaneous/genetics ; Amyloid beta-Protein Precursor/metabolism ; Animals ; Disease Models, Animal ; Drosophila Proteins ; Gene Expression Regulation, Enzymologic/drug effects ; Gene Expression Regulation, Enzymologic/genetics ; Mice ; Mice, Mutant Strains ; Mitochondria/drug effects ; Mitochondria/enzymology ; Mitochondrial Diseases/genetics ; Mitochondrial Diseases/pathology ; Mitochondrial Diseases/prevention & control ; Motor Neurons/drug effects ; Motor Neurons/pathology ; Multienzyme Complexes/metabolism ; NADPH Dehydrogenase/metabolism ; Neuroprotective Agents/therapeutic use ; Nitric Oxide Synthase Type I/genetics ; Nitric Oxide Synthase Type I/metabolism ; Progesterone/therapeutic use ; RNA, Messenger/metabolism ; RNA-Binding Proteins ; Spinal Cord/drug effects ; Spinal Cord/pathology ; Spinal Cord/ultrastructure ; Subcellular Fractions/drug effects ; Subcellular Fractions/metabolism ; Superoxide Dismutase/metabolism ; Vesicular Transport Proteins/genetics
Chemical Substances	Amyloid beta-Protein Precursor ; Drosophila Proteins ; Multienzyme Complexes ; Neuroprotective Agents ; RNA, Messenger ; RNA-Binding Proteins ; Vesicular Transport Proteins ; Vps54 protein, mouse ; nos protein, Drosophila (142661-95-8) ; Progesterone (4G7DS2Q64Y) ; Nitric Oxide Synthase Type I (EC 1.14.13.39) ; Superoxide Dismutase (EC 1.15.1.1) ; NADPH Dehydrogenase (EC 1.6.99.1)
Language	English
Publishing date	2012-04-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80158-6
ISSN	1471-4159 ; 0022-3042 ; 1474-1644
ISSN (online)	1471-4159
ISSN	0022-3042 ; 1474-1644
DOI	10.1111/j.1471-4159.2012.07753.x
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Article ; Online: The LRRK2 G2019S mutation in a series of Argentinean patients with Parkinson's disease: clinical and demographic characteristics.

Gatto, Emilia Mabel / Parisi, Virginia / Converso, Daniela Paola / Poderoso, Juan José / Carreras, María Cecilia / Martí-Massó, José Felix / Paisán-Ruiz, Coro

Neuroscience letters

2013 Volume 537, Page(s) 1–5

Abstract: Objectives: To determine clinical characteristics and frequency of leucine-rich repeat kinase 2 gene (LRRK2) mutations in a cohort of patients with Parkinson's disease (PD) from Argentina.: Background: Variation in the LRRK2 gene represents the most ... ...

Abstract	Objectives: To determine clinical characteristics and frequency of leucine-rich repeat kinase 2 gene (LRRK2) mutations in a cohort of patients with Parkinson's disease (PD) from Argentina. Background: Variation in the LRRK2 gene represents the most common genetic determinant of PD, only few data are available from Latin-America. Design/methods: Informed consent was obtained and all studies were approved by the Institutional Review Boards. Fifty five consecutive PD patients were recruited. A structured interview and neurological examination were used to collect demographic and clinical information. Blood samples were obtained and DNA extracted from patient venous blood. All LRRK2 exons from 25 exon to 51 exon were screened in all patients. Results: Clinical and molecular data of 55 patients with PD were analyzed. Mean age was 68.8±10.6 years. Jewish and Basque ancestries were found positive in 9 and 7 patients, respectively; family history of PD was identified in 16 patients. The G2019S mutation was present in 3 Ashkenazi Jewish subjects (5.45%); all of them reported family history of PD in first-degree relatives. Although Argentina possesses one of the most important Basque communities outside Spain, non R1414G mutation was identified in this cohort. Eleven single polymorphisms (SNP) were identified in this cohort. The mean age at onset was higher in G2019S mutation carriers than non-carriers (66.67 vs 58.78 years). Asymmetrical tremor as initial symptom and non-motor symptoms occurred at similar frequencies in both groups. The G2019S mutation carriers showed a non significant increase in dyskinesias, and 2/3 developed Dopamine Dysregulation Syndrome and visual hallucinations. Systemic disorder identified in G2019S mutation carriers included: celiac disease, hypothyroidism, Hashimoto's Thyroiditis and arterial hypertension. Conclusions: The prevalence of LRRK2 G2019S mutation in this Argentinean cohort was similar to other international series, with a higher prevalence in Ashkenazi Jewish. The phenotype was indistinguishable from patients with idiopathic PD. Interestingly, we identified immune mediated disorders in two PD patients carrying the G2019S mutation. Within this context, recent studies have identified full-length LRRK2 as a relatively common constituent of many cell types in the immune system including human peripheral blood mononuclear cells. Nevertheless, a casual association could not be excluded and the analysis of more extensive series is required.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Argentina ; European Continental Ancestry Group ; Female ; Heterozygote ; Humans ; Jews ; Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 ; Male ; Middle Aged ; Mutation ; Parkinson Disease/ethnology ; Parkinson Disease/genetics ; Parkinson Disease/physiopathology ; Pedigree ; Protein-Serine-Threonine Kinases/genetics
Chemical Substances	LRRK2 protein, human (EC 2.7.11.1) ; Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 (EC 2.7.11.1) ; Protein-Serine-Threonine Kinases (EC 2.7.11.1)
Language	English
Publishing date	2013-03-14
Publishing country	Ireland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	194929-9
ISSN	1872-7972 ; 0304-3940
ISSN (online)	1872-7972
ISSN	0304-3940
DOI	10.1016/j.neulet.2013.01.011
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