LIVIVO - Search results -

Search results

Result 1 - 10 of total 1075

Search options

Article ; Online: The N-Terminal Domain of Aβ

Meirovitch, Eva / Liang, Zhichun / Freed, Jack H

The journal of physical chemistry. B

2022 Volume 126, Issue 6, Page(s) 1202–1211

Abstract: We have developed the stochastic microscopic-order-macroscopic-disorder (MOMD) approach for elucidating dynamic structures in the solid-state ... ...

Abstract	We have developed the stochastic microscopic-order-macroscopic-disorder (MOMD) approach for elucidating dynamic structures in the solid-state from
MeSH term(s)	Amyloid/chemistry ; Amyloid beta-Peptides/chemistry ; Diffusion ; Magnetic Resonance Imaging ; Magnetic Resonance Spectroscopy ; Protein Aggregates
Chemical Substances	Amyloid ; Amyloid beta-Peptides ; Protein Aggregates
Language	English
Publishing date	2022-02-05
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ISSN	1520-5207
ISSN (online)	1520-5207
DOI	10.1021/acs.jpcb.1c10131
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: An analysis of double-quantum coherence ESR in an N-spin system: Analytical expressions and predictions.

Sinha Roy, Aritro / Marohn, John A / Freed, Jack H

The Journal of chemical physics

2024 Volume 160, Issue 13

Abstract: ... we have derived analytical expressions for the DQC signal for a system with N-dipolar coupled spin-1/2 ...

Abstract	Electron spin resonance pulsed dipolar spectroscopy (PDS) has become popular in protein 3D structure analysis. PDS studies yield distance distributions between a pair or multiple pairs of spin probes attached to protein molecules, which can be used directly in structural studies or as constraints in theoretical predictions. Double-quantum coherence (DQC) is a highly sensitive and accurate PDS technique to study protein structures in the solid state and under physiologically relevant conditions. In this work, we have derived analytical expressions for the DQC signal for a system with N-dipolar coupled spin-1/2 particles in the solid state. The expressions are integrated over the relevant spatial parameters to obtain closed form DQC signal expressions. These expressions contain the concentration-dependent "instantaneous diffusion" and the background signal. For micromolar and lower concentrations, these effects are negligible. An approximate analysis is provided for cases of finite pulses. The expressions obtained in this work should improve the analysis of DQC experimental data significantly, and the analytical approach could be extended easily to a wide range of magnetic resonance phenomena.
Language	English
Publishing date	2024-04-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	3113-6
ISSN	1089-7690 ; 0021-9606
ISSN (online)	1089-7690
ISSN	0021-9606
DOI	10.1063/5.0200054
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 4' 49/16: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article: The N-Terminal Domain of Aβ₄₀-Amyloid Fibril: The MOMD Perspective of its Dynamic Structure from NMR Lineshape Analysis

Meirovitch, Eva / Liang, Zhichun / Freed, Jack H.

Journal of physical chemistry. 2022 Feb. 05, v. 126, no. 6

2022

Abstract: ... amyloid fibril. Here, we apply it to the N-terminal domain of this fibril. We find that the dynamic structures ... independent. For the core domain, MOMD and MSM disagree on the character of the dynamic structure. For the N ...

Abstract	We have developed the stochastic microscopic-order-macroscopic-disorder (MOMD) approach for elucidating dynamic structures in the solid-state from ²H NMR lineshapes. In MOMD, the probe experiences an effective/collective motional mode. The latter is described by a potential, u, which represents the local spatial-restrictions, a local-motional diffusion tensor, R, and key features of local geometry. Previously we applied MOMD to the well-structured core domain of the 3-fold-symmetric twisted polymorph of the Aβ₄₀-amyloid fibril. Here, we apply it to the N-terminal domain of this fibril. We find that the dynamic structures of the two domains are largely similar but differ in the magnitude and complexity of the key physical parameters. This interpretation differs from previous multisimple-mode (MSM) interpretations of the same experimental data. MSM used for the two domains different combinations of simple motional modes taken to be independent. For the core domain, MOMD and MSM disagree on the character of the dynamic structure. For the N-terminal domain, they even disagree on whether this chain segment is structurally ordered (MOMD finds that it is), and whether it undergoes a phase transition at 260 K where bulklike water located in the fibril matrix freezes (MOMD finds that it does not). These are major differences associated with an important system. While the MOMD description is a physically sound one, there are drawbacks in the MSM descriptions. The results obtained in this study promote our understanding of the dynamic structure of protein aggregates. Thus, they contribute to the effort to pharmacologically control neurodegenerative disorders believed to be caused by such aggregates.
Keywords	freezing ; geometry ; journals ; neurodegenerative diseases ; protein aggregates ; protein structure
Language	English
Dates of publication	2022-0205
Size	p. 1202-1211.
Publishing place	American Chemical Society
Document type	Article
ISSN	1520-5207
DOI	10.1021/acs.jpcb.1c10131
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Book ; Online ; E-Book: Assessment of communication disorders in adults

Hegde, M. N. / Freed, Donald B.

resources and protocols

2022

Abstract: Assessment of Communication Disorders in Adults: Resources and Protocols, Third Edition offers a unique combination of scholarly information, invaluable resources, and time-saving protocols on assessment of communication disorders in adults. Most ... ...

Author's details	M.N. Hegde,PhD, Don Freed, PhD
Abstract	Assessment of Communication Disorders in Adults: Resources and Protocols, Third Edition offers a unique combination of scholarly information, invaluable resources, and time-saving protocols on assessment of communication disorders in adults. Most resource books offer limited research and scholarly information, thus making them unsuitable as textbooks for academic courses on assessment and diagnosis. Similarly, most traditional textbooks do not include practical, easy-to-use, and time-saving resources and protocols that the practicing clinicians can readily use during assessment sessions. By combining the strengths of traditional textbooks with newer assessment resources and protocols, this one-of-a-kind book offers a single, comprehensive source that is suitable as a textbook and useful as a practical clinical resource. This bestselling and trusted text: Covers the full range of communication disorders in adults, from aphasia to voice disorders Gives a comprehensive outline of basic assessment procedures Provides a set of protocols that are necessary to assess any communication disorder in adults Addresses the multicultural issues in assessing communication disorders in adults and offers an integrated assessment approach that includes the most desirable features of the traditional and several alternative approaches Contains two chapters for each disorder: one on resources that offers scholarly and research background on the disorder and one on resources that describes practical procedures and protocols that save preparation time and effort for the clinician New to the Third Edition: Expanded emphasis and specific guidelines on making a correct differential diagnosis Latest research on the characteristics of communication disorders in adults Review of recent trends on diagnostic assessment with critical recommendations for students and clinicians Updated epidemiological research on communication disorders Revised text to offer more succinct information on assessment tools and diagnostic criteria The latest standardized and informal assessment instruments Student-friendly, step-by-step instructions on how to conduct initial interviews and share final assessment results with patients in each protocol chapter
Keywords	Communicative disorders/Diagnosis ; Communicative disorders/Prevention/Needs assessment ; Medical protocols
Subject code	616.855
Language	English
Dates of publication	2022-2022
Size	1 online resource (446 pages) :, illustrations
Edition	Third edition.
Publisher	Plural Publishing, Inc
Publishing place	San Diego, CA
Document type	Book ; Online ; E-Book
Note	"The third edition of this book, Assessment of Communication Disorders in Adults, is a companion volume to the third edition of the Assessment of Communication Disorders in Children (Hegde & Pomaville, 2017)"--ECIP galley.
Remark	Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
ISBN	1-63550-195-4 ; 9781635502633 ; 1635502632 ; 978-1-63550-195-7
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article: Elevated concentration of N-CAM VASE isoforms in schizophrenia.

Vawter, M P / Frye, M A / Hemperly, J J / VanderPutten, D M / Usen, N / Doherty, P / Saffell, J L / Issa, F / Post, R M / Wyatt, R J / Freed, W J

Journal of psychiatric research

2000 Volume 34, Issue 1, Page(s) 25–34

Abstract: Neural cell adhesion molecule (N-CAM) is a cell recognition molecule, four major isoforms (180, 140 ... 120, and 105-115 kDa) of which are present in brain. N-CAM has several roles in cellular organization ... and CNS development. Previously we have found an elevation in CSF N-CAM 120 kDa in the CSF of patients ...

Abstract	Neural cell adhesion molecule (N-CAM) is a cell recognition molecule, four major isoforms (180, 140, 120, and 105-115 kDa) of which are present in brain. N-CAM has several roles in cellular organization and CNS development. Previously we have found an elevation in CSF N-CAM 120 kDa in the CSF of patients with schizophrenia, bipolar disorder, and depression. We now report an increase in the variable alternative spliced exon (VASE), a 10 amino acid sequence inserted into the fourth N-CAM domain, in the CSF of patients with schizophrenia, but not in bipolar disorder or depression. VASE-immunoreactive (VASE-ir) bands were measured in CSF from patients with schizophrenia (n = 14), bipolar disorder I (n = 7), bipolar disorder II (n = 9), unipolar depression (n = 17) and matched controls (n = 37) by Western immunoblotting. Three VASE-ir bands were distinguished in lumbar CSF corresponding to heavy (165 kDa), medium (155 kDa) and low (140 kDa) MW. A logarithmic transformation was applied to the VASE protein units and analyzed with a MANOVA. There was a 51% and 45% increase in VASE heavy (p = 0.0008) and medium (p = 0.04) MW protein, respectively, in patients with schizophrenia as compared with normal controls. Current neuroleptic treatment in patients with schizophrenia had no effect on CSF VASE concentrations. VASE concentration correlated significantly with behavioral ratings in patients with schizophrenia but not affective disorders. Thus, VASE immunoreactivity is increased in schizophrenia but not in affective disorders. These results provide further evidence of an abnormality of N-CAM protein in chronic schizophrenia and suggest differences between schizophrenia and affective disorders in regulation of N-CAM.
MeSH term(s)	Alternative Splicing/genetics ; Antibody Specificity/genetics ; Bipolar Disorder/diagnosis ; Blotting, Western ; Depressive Disorder/diagnosis ; Exons/genetics ; Humans ; Immune Sera ; Immunoproteins/cerebrospinal fluid ; Neural Cell Adhesion Molecules/cerebrospinal fluid ; Neural Cell Adhesion Molecules/genetics ; Protein Isoforms/cerebrospinal fluid ; Protein Isoforms/genetics ; Psychiatric Status Rating Scales ; Recombinant Fusion Proteins/cerebrospinal fluid ; Schizophrenia/cerebrospinal fluid ; Schizophrenia/diagnosis ; Schizophrenia/genetics
Chemical Substances	Immune Sera ; Immunoproteins ; Neural Cell Adhesion Molecules ; Protein Isoforms ; Recombinant Fusion Proteins
Language	English
Publishing date	2000-01
Publishing country	England
Document type	Journal Article
ZDB-ID	3148-3
ISSN	1879-1379 ; 0022-3956
ISSN (online)	1879-1379
ISSN	0022-3956
DOI	10.1016/s0022-3956(99)00026-6
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ui I Zs.243: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Antibodies to the buried N terminus of rhinovirus VP4 exhibit cross-serotypic neutralization.

Katpally, Umesh / Fu, Tong-Ming / Freed, Daniel C / Casimiro, Danilo R / Smith, Thomas J

Journal of virology

2009 Volume 83, Issue 14, Page(s) 7040–7048

Abstract: ... and transiently displays the buried N termini of viral protein 1 (VP1) and VP4. Here, further evidence ... for this "breathing" phenomenon is presented, using antibodies to several peptides representing the N terminus of VP4 ... Furthermore, there is evidence that the N termini of VP4 are interacting with each other upon extrusion ...

Abstract	Development of a vaccine for the common cold has been thwarted by the fact that there are more than 100 serotypes of human rhinovirus (HRV). We previously demonstrated that the HRV14 capsid is dynamic and transiently displays the buried N termini of viral protein 1 (VP1) and VP4. Here, further evidence for this "breathing" phenomenon is presented, using antibodies to several peptides representing the N terminus of VP4. The antibodies form stable complexes with intact HRV14 virions and neutralize infectivity. Since this region of VP4 is highly conserved among all of the rhinoviruses, antiviral activity by these anti-VP4 antibodies is cross-serotypic. The antibodies inhibit HRV16 infectivity in a temperature- and time-dependent manner consistent with the breathing behavior. Monoclonal and polyclonal antibodies raised against the 30-residue peptide do not react with peptides shorter than 24 residues, suggesting that these peptides are adopting three-dimensional conformations that are highly dependent upon the length of the peptide. Furthermore, there is evidence that the N termini of VP4 are interacting with each other upon extrusion from the capsid. A Ser5Cys mutation in VP4 yields an infectious virus that forms cysteine cross-links in VP4 when the virus is incubated at room temperature but not at 4 degrees C. The fact that all of the VP4s are involved in this cross-linking process strongly suggests that VP4 forms specific oligomers upon extrusion. Together these results suggest that it may be possible to develop a pan-serotypic peptide vaccine to HRV, but its design will likely require details about the oligomeric structure of the exposed termini.
MeSH term(s)	Amino Acid Motifs ; Amino Acid Sequence ; Animals ; Antibodies, Viral/immunology ; Capsid Proteins/chemistry ; Capsid Proteins/genetics ; Capsid Proteins/immunology ; Cell Line ; Cross Reactions ; Epitope Mapping ; Humans ; Molecular Sequence Data ; Neutralization Tests ; Picornaviridae Infections/immunology ; Picornaviridae Infections/virology ; Rabbits ; Rhinovirus/chemistry ; Rhinovirus/genetics ; Rhinovirus/immunology
Chemical Substances	Antibodies, Viral ; Capsid Proteins
Language	English
Publishing date	2009-04-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	80174-4
ISSN	1098-5514 ; 0022-538X
ISSN (online)	1098-5514
ISSN	0022-538X
DOI	10.1128/JVI.00557-09
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 609: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Evidence of a role for soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) machinery in HIV-1 assembly and release.

Joshi, Anjali / Garg, Himanshu / Ablan, Sherimay D / Freed, Eric O

The Journal of biological chemistry

2011 Volume 286, Issue 34, Page(s) 29861–29871

Abstract: ... The soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins constitute ...

Abstract	Retrovirus assembly is a complex process that requires the orchestrated participation of viral components and host-cell factors. The concerted movement of different viral proteins to specific sites in the plasma membrane allows for virus particle assembly and ultimately budding and maturation of infectious virions. The soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins constitute the minimal machinery that catalyzes the fusion of intracellular vesicles with the plasma membrane, thus regulating protein trafficking. Using siRNA and dominant negative approaches we demonstrate here that generalized disruption of the host SNARE machinery results in a significant reduction in human immunodeficiency virus type 1 (HIV-1) and equine infectious anemia virus particle production. Further analysis of the mechanism involved revealed a defect at the level of HIV-1 Gag localization to the plasma membrane. Our findings demonstrate for the first time a role of SNARE proteins in HIV-1 assembly and release, likely by affecting cellular trafficking pathways required for Gag transport and association with the plasma membrane.
MeSH term(s)	Biological Transport/genetics ; Cell Membrane/genetics ; Cell Membrane/metabolism ; HIV-1/physiology ; HeLa Cells ; Humans ; Infectious Anemia Virus, Equine/physiology ; SNARE Proteins/genetics ; SNARE Proteins/metabolism ; Virus Assembly/physiology ; Virus Release/physiology ; gag Gene Products, Human Immunodeficiency Virus/genetics ; gag Gene Products, Human Immunodeficiency Virus/metabolism
Chemical Substances	SNARE Proteins ; gag Gene Products, Human Immunodeficiency Virus
Language	English
Publishing date	2011-06-16
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Intramural
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1074/jbc.M111.241521
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uc I Zs.108: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Does the association between objective and subjective memory vary by age among healthy older adults?

Freed, Sara A / Sprague, Briana N / Ross, Lesley A

Neuropsychology, development, and cognition. Section B, Aging, neuropsychology and cognition

2022 Volume 31, Issue 2, Page(s) 249–262

Abstract: Subjective memory is commonly used as an indicator of older adults' objective memory in clinical screening; however, the correspondence between subjective and objective memory across different ages is unclear. The current study examined age-varying ... ...

Abstract	Subjective memory is commonly used as an indicator of older adults' objective memory in clinical screening; however, the correspondence between subjective and objective memory across different ages is unclear. The current study examined age-varying associations between subjective and objective memory in a cross-sectional sample of healthy older adults from the Advanced Cognitive Training for Independent and Vital Elderly (ACTIVE) study (
MeSH term(s)	Humans ; Aged ; Cross-Sectional Studies ; Health Status ; Memory Disorders/psychology
Language	English
Publishing date	2022-11-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	1482447-4
ISSN	1744-4128 ; 1382-5585
ISSN (online)	1744-4128
ISSN	1382-5585
DOI	10.1080/13825585.2022.2143471
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: N,N-dimethylglycine, betaine, and seizures.

Freed, W J

Archives of neurology

1984 Volume 41, Issue 11, Page(s) 1129–1130

MeSH term(s)	Betaine/therapeutic use ; Epilepsy/drug therapy ; Glycine/analogs & derivatives ; Humans ; Sarcosine/analogs & derivatives ; Sarcosine/therapeutic use
Chemical Substances	Betaine (3SCV180C9W) ; dimethylglycine (7797M4CPPA) ; Glycine (TE7660XO1C) ; Sarcosine (Z711V88R5F)
Language	English
Publishing date	1984-11
Publishing country	United States
Document type	Letter
ZDB-ID	80049-1
ISSN	1538-3687 ; 0003-9942
ISSN (online)	1538-3687
ISSN	0003-9942
DOI	10.1001/archneur.1984.04050220023004
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ui II Zs.191: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Data Processing and Germline Variant Calling with the Sentieon Pipeline.

Aldana, Rafael / Freed, Donald

Methods in molecular biology (Clifton, N.J.)

2022 Volume 2493, Page(s) 1–19

Abstract: Public and private genomic sequencing initiatives generate ever-increasing amounts of genomic data creating a need for improved solutions for genomics data processing (Stephens et al.PLoS Biol 13:e1002195, 2015). The ... ...

Abstract	Public and private genomic sequencing initiatives generate ever-increasing amounts of genomic data creating a need for improved solutions for genomics data processing (Stephens et al.PLoS Biol 13:e1002195, 2015). The Sentieon
MeSH term(s)	Genomics/methods ; Germ Cells ; High-Throughput Nucleotide Sequencing/methods ; Software
Language	English
Publishing date	2022-06-25
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-2293-3_1
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED