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  1. Article: Uncovering the Inhibitory Molecular Mechanism of Pomegranate Peel to Urinary Bladder Urothelial Carcinoma Using Proteomics Techniques.

    Huang, Kuan-Hua / Chang, Ching-Ping / Chien, Lan-Hsiang / Li, Chien-Feng / Tang, Ling-Yu / Chan, Yu-Yi / Wu, Ting-Feng

    Life (Basel, Switzerland)

    2022  Volume 12, Issue 11

    Abstract: Pomegranate ( ...

    Abstract Pomegranate (
    Language English
    Publishing date 2022-11-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662250-6
    ISSN 2075-1729
    ISSN 2075-1729
    DOI 10.3390/life12111839
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  2. Article ; Online: SARS-CoV-2 NSP13 helicase suppresses interferon signaling by perturbing JAK1 phosphorylation of STAT1

    Sin-Yee Fung / Kam-Leung Siu / Huayue Lin / Ching-Ping Chan / Man Lung Yeung / Dong-Yan Jin

    Cell & Bioscience, Vol 12, Iss 1, Pp 1-

    2022  Volume 12

    Abstract: Abstract Background SARS-CoV-2 is the causative agent of COVID-19. Overproduction and release of proinflammatory cytokines are the underlying cause of severe COVID-19. Treatment of this condition with JAK inhibitors is a double-edged sword, which might ... ...

    Abstract Abstract Background SARS-CoV-2 is the causative agent of COVID-19. Overproduction and release of proinflammatory cytokines are the underlying cause of severe COVID-19. Treatment of this condition with JAK inhibitors is a double-edged sword, which might result in the suppression of proinflammatory cytokine storm and the concurrent enhancement of viral infection, since JAK signaling is essential for host antiviral response. Improving the current JAK inhibitor therapy requires a detailed molecular analysis on how SARS-CoV-2 modulates interferon (IFN)-induced activation of JAK-STAT signaling. Results In this study, we focused on the molecular mechanism by which SARS-CoV-2 NSP13 helicase suppresses IFN signaling. Expression of SARS-CoV-2 NSP13 alleviated transcriptional activity driven by type I and type II IFN-responsive enhancer elements. It also prevented nuclear translocation of STAT1 and STAT2. The suppression of NSP13 on IFN signaling occurred at the step of STAT1 phosphorylation. Nucleic acid binding-defective mutant K345A K347A and NTPase-deficient mutant E375A of NSP13 were found to have largely lost the ability to suppress IFN-β-induced STAT1 phosphorylation and transcriptional activation, indicating the requirement of the helicase activity for NSP13-mediated inhibition of STAT1 phosphorylation. NSP13 did not interact with JAK1 nor prevent STAT1-JAK1 complex formation. Mechanistically, NSP13 interacted with STAT1 to prevent JAK1 kinase from phosphorylating STAT1. Conclusion SARS-CoV-2 NSP13 helicase broadly suppresses IFN signaling by targeting JAK1 phosphorylation of STAT1.
    Keywords SARS-CoV-2 ; COVID-19 ; NSP13 ; Helicase ; JAK1 ; STAT1 ; Biotechnology ; TP248.13-248.65 ; Biology (General) ; QH301-705.5 ; Biochemistry ; QD415-436
    Subject code 570
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: SARS-CoV-2 NSP13 helicase suppresses interferon signaling by perturbing JAK1 phosphorylation of STAT1.

    Fung, Sin-Yee / Siu, Kam-Leung / Lin, Huayue / Chan, Ching-Ping / Yeung, Man Lung / Jin, Dong-Yan

    Cell & bioscience

    2022  Volume 12, Issue 1, Page(s) 36

    Abstract: Background: SARS-CoV-2 is the causative agent of COVID-19. Overproduction and release of proinflammatory cytokines are the underlying cause of severe COVID-19. Treatment of this condition with JAK inhibitors is a double-edged sword, which might result ... ...

    Abstract Background: SARS-CoV-2 is the causative agent of COVID-19. Overproduction and release of proinflammatory cytokines are the underlying cause of severe COVID-19. Treatment of this condition with JAK inhibitors is a double-edged sword, which might result in the suppression of proinflammatory cytokine storm and the concurrent enhancement of viral infection, since JAK signaling is essential for host antiviral response. Improving the current JAK inhibitor therapy requires a detailed molecular analysis on how SARS-CoV-2 modulates interferon (IFN)-induced activation of JAK-STAT signaling.
    Results: In this study, we focused on the molecular mechanism by which SARS-CoV-2 NSP13 helicase suppresses IFN signaling. Expression of SARS-CoV-2 NSP13 alleviated transcriptional activity driven by type I and type II IFN-responsive enhancer elements. It also prevented nuclear translocation of STAT1 and STAT2. The suppression of NSP13 on IFN signaling occurred at the step of STAT1 phosphorylation. Nucleic acid binding-defective mutant K345A K347A and NTPase-deficient mutant E375A of NSP13 were found to have largely lost the ability to suppress IFN-β-induced STAT1 phosphorylation and transcriptional activation, indicating the requirement of the helicase activity for NSP13-mediated inhibition of STAT1 phosphorylation. NSP13 did not interact with JAK1 nor prevent STAT1-JAK1 complex formation. Mechanistically, NSP13 interacted with STAT1 to prevent JAK1 kinase from phosphorylating STAT1.
    Conclusion: SARS-CoV-2 NSP13 helicase broadly suppresses IFN signaling by targeting JAK1 phosphorylation of STAT1.
    Language English
    Publishing date 2022-03-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 2593367-X
    ISSN 2045-3701
    ISSN 2045-3701
    DOI 10.1186/s13578-022-00770-1
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  4. Article: Inter- and intra-specific variations in phenotypic traits of

    Tang, Wen-Chien / Wang, Liang-Hsuan / Chan, Jiun-Jie / Goh, Reun-Ping / Wu, Yea-Fang / Chu, Chia-Ching

    Plant disease

    2024  

    Abstract: ... ...

    Abstract Pectobacterium
    Language English
    Publishing date 2024-03-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-10-23-2130-RE
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  5. Article ; Online: The feasibility of emergency medical technicians performing intermittent high-quality cardiopulmonary resuscitation.

    Chang, Chun-Hao / Hsu, Yi-Ju / Li, Fang / Chan, Yuan-Shuo / Lo, Ching-Ping / Peng, Guan-Jian / Ho, Chin-Shan / Huang, Chi-Chang

    International journal of medical sciences

    2021  Volume 18, Issue 12, Page(s) 2615–2623

    Abstract: Background: ...

    Abstract Background:
    MeSH term(s) Adult ; Body Composition ; Cardiopulmonary Resuscitation/methods ; Emergency Medical Technicians ; Feasibility Studies ; Firefighters ; Humans ; Male ; Out-of-Hospital Cardiac Arrest/therapy ; Taiwan ; Time Factors ; Treatment Outcome ; Young Adult
    Language English
    Publishing date 2021-04-29
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 2151424-0
    ISSN 1449-1907 ; 1449-1907
    ISSN (online) 1449-1907
    ISSN 1449-1907
    DOI 10.7150/ijms.59757
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  6. Article ; Online: Reply to Lai and Wei.

    Su, Chia Ping / Chan, K Arnold / Huang, Ching Tai / Fang, Chi Tai

    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

    2022  Volume 75, Issue 6, Page(s) 1113

    Language English
    Publishing date 2022-04-26
    Publishing country United States
    Document type Letter ; Comment
    ZDB-ID 1099781-7
    ISSN 1537-6591 ; 1058-4838
    ISSN (online) 1537-6591
    ISSN 1058-4838
    DOI 10.1093/cid/ciac336
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    In stock of ZB MED Cologne/Königswinter

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  7. Article ; Online: Reply to Chang et al.

    Su, Chia-Ping / Chan, K Arnold / Huang, Ching-Tai / Fang, Chi-Tai

    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

    2022  Volume 75, Issue 9, Page(s) 1677

    Language English
    Publishing date 2022-06-15
    Publishing country United States
    Document type Letter
    ZDB-ID 1099781-7
    ISSN 1537-6591 ; 1058-4838
    ISSN (online) 1537-6591
    ISSN 1058-4838
    DOI 10.1093/cid/ciac464
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  8. Article ; Online: Climate velocities and species tracking in global mountain regions.

    Chan, Wei-Ping / Lenoir, Jonathan / Mai, Guan-Shuo / Kuo, Hung-Chi / Chen, I-Ching / Shen, Sheng-Feng

    Nature

    2024  

    Abstract: Mountain ranges contain high concentrations of endemic species and are indispensable refugia for lowland species that are facing anthropogenic climate ... ...

    Abstract Mountain ranges contain high concentrations of endemic species and are indispensable refugia for lowland species that are facing anthropogenic climate change
    Language English
    Publishing date 2024-03-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 120714-3
    ISSN 1476-4687 ; 0028-0836
    ISSN (online) 1476-4687
    ISSN 0028-0836
    DOI 10.1038/s41586-024-07264-9
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  9. Article ; Online: Uncovering the Inhibitory Molecular Mechanism of Pomegranate Peel to Urinary Bladder Urothelial Carcinoma Using Proteomics Techniques

    Kuan-Hua Huang / Ching-Ping Chang / Lan-Hsiang Chien / Chien-Feng Li / Ling-Yu Tang / Yu-Yi Chan / Ting-Feng Wu

    Life, Vol 12, Iss 1839, p

    2022  Volume 1839

    Abstract: Pomegranate ( Punica granatum L.) fruit demonstrates the repressive effectiveness of many tumors. Our previous studies showed that the PEP (pomegranate peel extract) E2 fraction obtained from the ethyl acetate layer of the pomegranate peel’s ethanol ... ...

    Abstract Pomegranate ( Punica granatum L.) fruit demonstrates the repressive effectiveness of many tumors. Our previous studies showed that the PEP (pomegranate peel extract) E2 fraction obtained from the ethyl acetate layer of the pomegranate peel’s ethanol extract exhibited the highest inhibitory activities to induce Urinary bladder urothelial carcinoma (UBUC) cell apoptosis. The ethyl acetate layer could lower the volume and weight of T24 tumors and initiate apoptosis in nude mice xenografted bladder tumors. In this study, we intended to clarify the inhibitory molecular process of Taiwanese local pomegranate peel to urinary bladder urothelial carcinoma using a proteomics strategy. Gel-based proteomics (two-dimensional gel electrophoresis coupled with tandem mass spectrometry) was used to get an insight into the molecular mechanisms initiated by PEPE2 to evoke bladder cancer cell apoptosis. We found eleven down-regulated and eight up-regulated proteins in PEPE2-treated T24 cells. Our results implied that these PEPE2-dysregulated proteins belong to cell apoptosis, cell proliferation, death receptor signaling, JAK/STAT signaling, the PPAR pathway, the PPARα/RXR α pathway, Rho family GTPase signaling, and RhoGDI signaling. In addition, HSP90 and PTP1B proteins, associated with apoptosis, were de-regulated in xenografted bladder tumors in nude mice fed with an ethyl acetate layer of ethanol extract. The findings above implied that pomegranate might be a potential chemopreventive resource for UBUC carcinogenesis.
    Keywords pomegranate ; proteomics ; HSP90 ; PTP1B ; bladder cancer ; urinary bladder urothelial carcinoma ; Science ; Q
    Subject code 616
    Language English
    Publishing date 2022-11-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
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  10. Article ; Online: Production of single-cycle infectious SARS-CoV-2 through a trans-complemented replicon.

    Cheung, Pak-Hin Hinson / Ye, Zi-Wei / Lui, Wai-Yin / Ong, Chon Phin / Chan, Pearl / Lee, Tak-Wang Terence / Tang, Tze-Tung / Yuen, Tin-Long / Fung, Sin-Yee / Cheng, Yun / Chan, Ching-Ping / Chan, Chi-Ping / Jin, Dong-Yan

    Journal of medical virology

    2022  Volume 94, Issue 12, Page(s) 6078–6090

    Abstract: Single-cycle infectious virus can elicit close-to-natural immune response and memory. One approach to generate single-cycle severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is through deletion of structural genes such as spike (S) and ... ...

    Abstract Single-cycle infectious virus can elicit close-to-natural immune response and memory. One approach to generate single-cycle severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is through deletion of structural genes such as spike (S) and nucleocapsid (N). Transcomplementation of the resulting ΔS or ΔN virus through enforced expression of S or N protein in the cells gives rise to a live but unproductive virus. In this study, ΔS and ΔN BAC clones were constructed and their live virions were rescued by transient expression of S and N proteins from the ancestral and the Omicron strains. ΔS and ΔN virions were visualized by transmission electron microscopy. Virion production of ΔS was more efficient than that of ΔN. The coated S protein from ΔS was delivered to infected cells in which the expression of N protein was also robust. In contrast, expression of neither S nor N was detected in ΔN-infected cells. ΔS underwent viral RNA replication, induced type I interferon (IFN) response, but did not form plaques. Despite RNA replication in cells, ΔS infection did not produce viral progeny in culture supernatant. Interestingly, viral RNA replication was not further enhanced upon overexpression of S protein. Taken together, our work provides a versatile platform for development of single-cycle vaccines for SARS-CoV-2.
    MeSH term(s) COVID-19 ; COVID-19 Vaccines ; Humans ; Interferon Type I/genetics ; RNA, Viral/genetics ; Replicon ; SARS-CoV-2/genetics ; Spike Glycoprotein, Coronavirus/genetics
    Chemical Substances COVID-19 Vaccines ; Interferon Type I ; RNA, Viral ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2022-08-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 752392-0
    ISSN 1096-9071 ; 0146-6615
    ISSN (online) 1096-9071
    ISSN 0146-6615
    DOI 10.1002/jmv.28057
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