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Article: Differences in oxidase and esterase activities involved in pheromone biosynthesis in two species ofChoristoneura.

Morse, D / Meighen, E A

2013 Volume 16, Issue 5, Page(s) 1485–1493

Abstract: The released pheromone and the glandular lipids, labeled with [(14)C]acetate, were analyzed fromChoristoneura orae andChoristoneura fumiferana budworm moths by thin-layer chromatography and autoradiography. Radiolabeled lipids in the gland appeared to be ...

Abstract	The released pheromone and the glandular lipids, labeled with [(14)C]acetate, were analyzed fromChoristoneura orae andChoristoneura fumiferana budworm moths by thin-layer chromatography and autoradiography. Radiolabeled lipids in the gland appeared to be identical in the two moths with both insects containing high amounts of 11-tetradecenyl acetates. In contrast, theC. orae moths released primarily labeled acetate ester and alcohol, and the spruce budworm moths (C. fumiferana) labeled aldehyde consistent with the known composition of their respective pheromones. The levels of the enzymes responsible for converting the acetate ester into aldehyde were found to be significantly lower in gland extracts fromC. orae moths than fromC. fumiferana moths. These results implicate an acetate esterase and an alcohol oxidase in controlling the composition of the pheromone blend released from closely related budworm species.
Language	English
Publishing date	2013-11-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	800130-3
ISSN	1573-1561 ; 0098-0331
ISSN (online)	1573-1561
ISSN	0098-0331
DOI	10.1007/BF01014083
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Article: Steady-state fluorescence and phosphorescence spectroscopic studies of bacterial luciferase tryptophan mutants.

Li, Z / Meighen, E A

Journal of fluorescence

2013 Volume 4, Issue 3, Page(s) 209–216

Abstract: Bacterial luciferase, which catalyzes the bioluminescence reaction in luminous bacteria, consists of two nonidentical polypeptides, α and β. Eight mutants of luciferase with each of the tryptophans replaced by tyrosine were generated by site-directed ... ...

Abstract	Bacterial luciferase, which catalyzes the bioluminescence reaction in luminous bacteria, consists of two nonidentical polypeptides, α and β. Eight mutants of luciferase with each of the tryptophans replaced by tyrosine were generated by site-directed mutagenesis and purified to homogeneity. The steady-state tryptophan fluorescence and low-temperature phosphorescence spectroscopic properties of these mutants were characterized. In some instances, mutation of only a single tryptophan residue resulted in large spectral changes. The tryptophan residues conserved in both the α and the β subunits exhibited distinct fluorescence emission properties, suggesting that these tryptophans have different local enviroments. The low-temperature phosphorescence data suggest that the tryptophans conserved in bot the α and the β subunits are not located at the subunit interface and/or involved in subunit interactions. The differences in the spectral properties of the mutants have provided useful information on the local environment of the individual tryptophan residues as well as on the quaternary structure of the protein.
Language	English
Publishing date	2013-04-22
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2016892-5
ISSN	1573-4994 ; 1053-0509
ISSN (online)	1573-4994
ISSN	1053-0509
DOI	10.1007/BF01878453
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Article: Lux C, D and E genes of the Vibrio fischeri luminescence operon code for the reductase, transferase, and synthetase enzymes involved in aldehyde biosynthesis.

Boylan, M / Miyamoto, C / Wall, L / Graham, A / Meighen, E

Photochemistry and photobiology

1989 Volume 49, Issue 5, Page(s) 681–688

Abstract: The lux C, D, and E genes of the Vibrio fischeri luminescence operon code for three polypeptides ... as in recombinant E. coli harboring the cloned genes by specific acylation with [3H]fatty acid, showing ... the lux E gene, the 42 kDa polypeptide was missing and the 54 kDa polypeptide could not be acylated ...

Abstract	The lux C, D, and E genes of the Vibrio fischeri luminescence operon code for three polypeptides of 54, 33, and 42 kDa, respectively, which are required for synthesis of the aldehyde substrate for the luminescent reaction. These polypeptides have been identified in V. fischeri and V. harveyi as well as in recombinant E. coli harboring the cloned genes by specific acylation with [3H]fatty acid, showing that they are components of a fatty acid reductase system with reductase, synthetase and transferase activities. By using glycerol in the assay and/or extraction buffer and decreasing the reducing agent, the levels of the acylation of the 54 and 42 kDa polypeptides have been greatly increased. As a consequence, it was possible to demonstrate that the 54 kDa polypeptide coded by the lux C gene has reductase activity. In a subclone missing the lux E gene, the 42 kDa polypeptide was missing and the 54 kDa polypeptide could not be acylated in vitro with tetradecanoic acid (+ATP) and only to a low level in vivo indicating that the synthetase enzyme, responsible for fatty acid activation, is coded by the lux E gene. In vitro acylation with tetradecanoyl CoA of the 33 kDa polypeptide coupled with the specific cleavage of acyl-ACP only in E. coli extracts transformed with DNA containing the lux D gene, demonstrated that the lux D gene coded for the transferase enzyme.
MeSH term(s)	Aldehydes ; Electrophoresis, Polyacrylamide Gel ; Genes, Bacterial ; Ligases/genetics ; Luminescence ; Operon ; Oxidoreductases/genetics ; Transferases/genetics ; Vibrio/enzymology ; Vibrio/genetics ; Vibrio/metabolism
Chemical Substances	Aldehydes ; Oxidoreductases (EC 1.-) ; Transferases (EC 2.-) ; Ligases (EC 6.-)
Language	English
Publishing date	1989-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	123540-0
ISSN	1751-1097 ; 0031-8655
ISSN (online)	1751-1097
ISSN	0031-8655
DOI	10.1111/j.1751-1097.1989.tb08441.x
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Article: Pheromone biosynthesis and role of functional groups in pheromone specificity.

Morse, D / Meighen, E

Journal of chemical ecology

2013 Volume 12, Issue 2, Page(s) 335–351

Abstract: The sex attractants for many Lepidoptera are long-chain, monounsaturated acetate esters, alcohols, and aldehydes. In vivo metabolic studies and enzyme analysis in vitro have provided evidence that the aldehyde pheromone of the eastern spruce budworm is ... ...

Abstract	The sex attractants for many Lepidoptera are long-chain, monounsaturated acetate esters, alcohols, and aldehydes. In vivo metabolic studies and enzyme analysis in vitro have provided evidence that the aldehyde pheromone of the eastern spruce budworm is synthesized de novo via an acetate ester precursor. Interconversion of the functional groups (ester, alcohol, aldehyde) can explain differences in the pheromone blends used by closely related species.
Language	English
Publishing date	2013-12-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	800130-3
ISSN	1573-1561 ; 0098-0331
ISSN (online)	1573-1561
ISSN	0098-0331
DOI	10.1007/BF01020560
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Article: Development of a bioluminescence assay for aldehyde pheromones of insects : I. Sensitivity and specificity.

Meighen, E A / Slessor, K N / Grant, G G

Journal of chemical ecology

2014 Volume 8, Issue 6, Page(s) 911–921

Abstract: A rapid, quantitative assay for long-chain aldehydes based on bacterial luminescence was developed. Significant luminescent responses were obtained with both saturated and unsaturated aldehydes of 12-18 carbons. Maximum responses were obtained with the ... ...

Abstract	A rapid, quantitative assay for long-chain aldehydes based on bacterial luminescence was developed. Significant luminescent responses were obtained with both saturated and unsaturated aldehydes of 12-18 carbons. Maximum responses were obtained with the 14- and 16-carbon compounds, including those that are known insect sex pheromones. The bioluminescent response was linearly related to the amount of aldehyde over a 10(4)-10(5) concentration range with as little as 0.1 pmol (∼20 pg) of aldehyde being detected. The bioluminescent assay represents a new quantitative tool for rapidly measuring aldehyde pheromones of insects.
Language	English
Publishing date	2014-01-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	800130-3
ISSN	1573-1561 ; 0098-0331
ISSN (online)	1573-1561
ISSN	0098-0331
DOI	10.1007/BF00987658
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Article ; Online: Fluorescent palladium(II) and platinum(II) NHC/1,2,3-triazole complexes: antiproliferative activity and selectivity against cancer cells.

Schlagintweit, Jonas F / Jakob, Christian H G / Meighen-Berger, Kevin / Gronauer, Thomas F / Weigert Muñoz, Angela / Weiß, Vanessa / Feige, Matthias J / Sieber, Stephan A / Correia, João D G / Kühn, Fritz E

Dalton transactions (Cambridge, England : 2003)

2021 Volume 50, Issue 6, Page(s) 2158–2166

Abstract: ... conditions two different ligand coordination modes are observed, i.e., bidentate solely coordinating via NHCs ...

Abstract	Fluorescent Pd(ii) and Pt(ii) complexes bearing 4-methylene-7-methoxycoumarin (MMC) and 2,6-diispropylphenyl (Dipp) substituted NHC/1,2,3-triazole hybrid ligands are described. Depending on the reaction conditions two different ligand coordination modes are observed, i.e., bidentate solely coordinating via NHCs or tetradentate coordinating via NHCs and 1,2,3-triazoles. All Dipp substituted complexes show antiproliferative activity against cervix (HeLa) and breast (MCF-7) human carcinoma cells. The activity significantly depends on the coordination mode, with the tetradentate motif being notably more effective (HeLa: IC50 = 3.9 μM to 4.7 μM; MCF-7: IC50 = 2.07 μM to 2.35 μM). Amongst the MMC series, only the Pd(ii) complex featuring the bidentate coordination mode is active against HeLa (IC50 = 6.1 μM). In contrast to its structurally related Dipp derivative (SI = 0.6), it shows a high selectivity for HeLa (SI > 16) compared to healthy skin cells (HaCaT). According to fluorescence microscopy, this compound is presumably located in late endosomes or lysosomes.
MeSH term(s)	Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Cell Line ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Coordination Complexes/chemistry ; Coordination Complexes/pharmacology ; Coumarins/chemistry ; Coumarins/pharmacology ; Fluorescence ; Humans ; Microscopy, Fluorescence ; Neoplasms/drug therapy ; Organometallic Compounds/chemistry ; Organometallic Compounds/pharmacology ; Palladium/chemistry ; Palladium/pharmacology ; Platinum/chemistry ; Platinum/pharmacology ; Triazoles/chemistry ; Triazoles/pharmacology
Chemical Substances	Antineoplastic Agents ; Coordination Complexes ; Coumarins ; Organometallic Compounds ; Triazoles ; Platinum (49DFR088MY) ; Palladium (5TWQ1V240M)
Language	English
Publishing date	2021-01-25
Publishing country	England
Document type	Journal Article
ZDB-ID	1472887-4
ISSN	1477-9234 ; 1364-5447 ; 0300-9246 ; 1477-9226
ISSN (online)	1477-9234 ; 1364-5447
ISSN	0300-9246 ; 1477-9226
DOI	10.1039/d0dt04114a
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Article: Development of a bioluminescence assay for aldehyde pheromones of insects : III. Analysis of airborne pheromone.

Szittner, R B / Morse, D / Grant, G G / Meighen, E A

Journal of chemical ecology

2014 Volume 8, Issue 6, Page(s) 935–945

Abstract: A newly developed bioluminescent assay was used to measure quantitatively the amount of (E)-11 ...

Abstract	A newly developed bioluminescent assay was used to measure quantitatively the amount of (E)-11-tetradecenal, the major component of the sex pheromone of the spruce budworm, trapped on Porapak Q(®). The bioluminescent response was linearly related to the amount of aldehyde either deposited on the absorbent or trapped from an airstream. However, the recovery of pheromone from Porapak was dependent on whether the air was prefiltered (through Porapak) or taken directly from the atmosphere. Furthermore, pheromone on Porapak was lost with time during the flow of air through the absorbent, indicating that trapping of aldehyde pheromone should be conducted for short periods of time for optimal recoveries. The applicability of the assay system for the rapid and direct measurement of the release rates of aldehyde pheromone lures was demonstrated for pheromone lures used for baiting spruce budworm traps.
Language	English
Publishing date	2014-01-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	800130-3
ISSN	1573-1561 ; 0098-0331
ISSN (online)	1573-1561
ISSN	0098-0331
DOI	10.1007/BF00987660
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Article: The impact of digital contact tracing on the SARS-CoV-2 pandemic-a comprehensive modelling study.

Pollmann, Tina R / Schönert, Stefan / Müller, Johannes / Pollmann, Julia / Resconi, Elisa / Wiesinger, Christoph / Haack, Christian / Shtembari, Lolian / Turcati, Andrea / Neumair, Birgit / Meighen-Berger, Stephan / Zattera, Giovanni / Neumair, Matthias / Apel, Uljana / Okolie, Augustine

EPJ data science

2021 Volume 10, Issue 1, Page(s) 37

Abstract: Contact tracing is one of several strategies employed in many countries to curb the spread of SARS-CoV-2. Digital contact tracing (DCT) uses tools such as cell-phone applications to improve tracing speed and reach. We model the impact of DCT on the ... ...

Abstract	Contact tracing is one of several strategies employed in many countries to curb the spread of SARS-CoV-2. Digital contact tracing (DCT) uses tools such as cell-phone applications to improve tracing speed and reach. We model the impact of DCT on the spread of the virus for a large epidemiological parameter space consistent with current literature on SARS-CoV-2. We also model DCT in combination with random testing (RT) and social distancing (SD). Modelling is done with two independently developed individual-based (stochastic) models that use the Monte Carlo technique, benchmarked against each other and against two types of deterministic models. For current best estimates of the number of asymptomatic SARS-CoV-2 carriers (approximately 40%), their contagiousness (similar to that of symptomatic carriers), the reproductive number before interventions (
Language	English
Publishing date	2021-07-20
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2705691-0
ISSN	2193-1127
ISSN	2193-1127
DOI	10.1140/epjds/s13688-021-00290-x
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Article: Autoinduction of light emission in different species of bioluminescent bacteria.

Meighen, E A

Luminescence : the journal of biological and chemical luminescence

1999 Volume 14, Issue 1, Page(s) 3–9

Abstract: The production of light in most luminescent bacteria lags behind cellular growth with induction of luminescence only occurring at high cell density. The maximum intensity and the cell density reached before induction of luminescence is highly dependent ... ...

Abstract	The production of light in most luminescent bacteria lags behind cellular growth with induction of luminescence only occurring at high cell density. The maximum intensity and the cell density reached before induction of luminescence is highly dependent on the bacterial species and the growth conditions including temperature, salt and nutrient composition as well as other experimental conditions. Although common N-acylhomoserine lactone autoinducers have been identified in Vibrio (Photobacterium) fischeri and Vibrio harveyi, most regulatory components are quite different. Recognition of the common as well as the different elements controlling luminescence in the diverse bacterial species is essential to understand the basis for high levels of light emission at the later stages of cellular growth.
MeSH term(s)	Light ; Luminescence ; Photobacterium/physiology ; Vibrio/physiology
Language	English
Publishing date	1999-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1470995-8
ISSN	1522-7243 ; 1522-7235 ; 1099-1271
ISSN (online)	1522-7243
ISSN	1522-7235 ; 1099-1271
DOI	10.1002/(SICI)1522-7243(199901/02)14:1<3::AID-BIO507>3.0.CO;2-4
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Article ; Online: Effects of Shade on Nitrogen and Phosphorus Acquisition in Cereal-Legume Intercropping Systems

Marney E. Isaac / Meighen Whitehead

Agriculture, Vol 2, Iss 1, Pp 12-

2012 Volume 24

Abstract: With increasing economic and environmental costs associated with fertilizer use, the need for low-input agroecological systems is on the rise. It is well documented that intercropping legumes can increase the supply of nutrients, through N2-fixation and ... ...

Abstract	With increasing economic and environmental costs associated with fertilizer use, the need for low-input agroecological systems is on the rise. It is well documented that intercropping legumes can increase the supply of nutrients, through N2-fixation and P mobilization. Concurrently, the integration of trees in the agricultural landscape shows increasing evidence of nutrient inputs through efficient biogeochemical cycles. However, little is known about the effects shade has on legume-crop performance. This research aims to determine whether intercropping of the legumes soybean (Glycine max L. Merr.) and alfalfa (Medicago sativa) with wheat (Triticum turgidum durum) is beneficial for performance, particularly under shady conditions associated with tree-based intercropping. Test species were cultivated in hydroponics with a broad nutrient solution and steady state addition of N for 3 weeks. Individual plants were transferred to rhizoboxes with a 2 mm zone of soil for 16 days under (i) full sun or (ii) shade to mimic light levels at the tree-crop interface. Under monocropping, shading was found to significantly decrease wheat biomass. Intercropping wheat with alfalfa under full sun had no negative effect on growth but did increase wheat P uptake as compared to monocropped wheat. In contrast, intercropping wheat with soybean under full sun decreased wheat biomass, suggesting competition. However, under shade, this competitive effect was mitigated, as wheat exhibited similar biomass and higher N and P shoot concentration when associated with soybean as compared to monocropped wheat under lower light levels. This effect may be attributed to reduced biomass of soybean combined with higher soybean N2-fixation under shade. Legume-based intercrops may increase nutrient supply and growth but these beneficial effects will be dependent on matching species selection to light levels under tree-based intercropping.
Keywords	intercropping ; 15N natural abundance ; N2-fixation ; Ontario ; rhizobox ; shade effects ; Agriculture (General) ; S1-972
Subject code	571
Language	English
Publishing date	2012-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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