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Article ; Online: 3D Printed Customizable Microsampling Devices for Neuroscience Applications.

Pysz, Patrick M / Hoskins, Julia K / Zou, Min / Stenken, Julie A

ACS chemical neuroscience

2023 Volume 14, Issue 18, Page(s) 3278–3287

Abstract: Multifunctional devices that incorporate chemical or physical measurements combined with ways to manipulate brain tissue via drug delivery, electrical stimulation, or light for optogenetics are desired by neuroscientists. The next generation in vivo ... ...

Abstract	Multifunctional devices that incorporate chemical or physical measurements combined with ways to manipulate brain tissue via drug delivery, electrical stimulation, or light for optogenetics are desired by neuroscientists. The next generation in vivo brain devices will likely utilize the extensive flexibility and rapid processing of 3D printing. This Perspective demonstrates how close we are to this reality for advanced neuroscience measurements. 3D printing provides the opportunity to improve microsampling-based devices in ways that have not been previously available. Not only can 3D printing be used for actual device creation, but it can also allow printing of peripheral objects necessary to assemble functional devices. The most probable 3D printing set up for microsampling devices with appropriate nm to μm feature size will likely require 2-photon polymerization-based printers. This Perspective describes the advantages and challenges for 3D printing of microsampling devices as an initial step to meet the next generation device needs of neuroscientists.
MeSH term(s)	Brain ; Drug Delivery Systems ; Electric Stimulation ; Neurosciences ; Printing, Three-Dimensional
Language	English
Publishing date	2023-08-30
Publishing country	United States
Document type	Journal Article ; Review ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
ISSN	1948-7193
ISSN (online)	1948-7193
DOI	10.1021/acschemneuro.3c00166
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Article ; Online: Enhancing resolution and contrast in fibre bundle-based fluorescence microscopy using generative adversarial network.

Ketabchi, Amir Mohammad / Morova, Berna / Uysalli, Yiğit / Aydin, Musa / Eren, Furkan / Bavili, Nima / Pysz, Dariusz / Buczynski, Ryszard / Kiraz, Alper

Journal of microscopy

2024

Abstract: Fibre bundle (FB)-based endoscopes are indispensable in biology and medical science due to their minimally invasive nature. However, resolution and contrast for fluorescence imaging are limited due to characteristic features of the FBs, such as low ... ...

Abstract	Fibre bundle (FB)-based endoscopes are indispensable in biology and medical science due to their minimally invasive nature. However, resolution and contrast for fluorescence imaging are limited due to characteristic features of the FBs, such as low numerical aperture (NA) and individual fibre core sizes. In this study, we improved the resolution and contrast of sample fluorescence images acquired using in-house fabricated high-NA FBs by utilising generative adversarial networks (GANs). In order to train our deep learning model, we built an FB-based multifocal structured illumination microscope (MSIM) based on a digital micromirror device (DMD) which improves the resolution and the contrast substantially compared to basic FB-based fluorescence microscopes. After network training, the GAN model, employing image-to-image translation techniques, effectively transformed wide-field images into high-resolution MSIM images without the need for any additional optical hardware. The results demonstrated that GAN-generated outputs significantly enhanced both contrast and resolution compared to the original wide-field images. These findings highlight the potential of GAN-based models trained using MSIM data to enhance resolution and contrast in wide-field imaging for fibre bundle-based fluorescence microscopy. Lay Description: Fibre bundle (FB) endoscopes are essential in biology and medicine but suffer from limited resolution and contrast for fluorescence imaging. Here we improved these limitations using high-NA FBs and generative adversarial networks (GANs). We trained a GAN model with data from an FB-based multifocal structured illumination microscope (MSIM) to enhance resolution and contrast without additional optical hardware. Results showed significant enhancement in contrast and resolution, showcasing the potential of GAN-based models for fibre bundle-based fluorescence microscopy.
Language	English
Publishing date	2024-04-02
Publishing country	England
Document type	Journal Article
ZDB-ID	219263-9
ISSN	1365-2818 ; 0022-2720
ISSN (online)	1365-2818
ISSN	0022-2720
DOI	10.1111/jmi.13296
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Article ; Online: Attachment and optimization of Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa biofilms to a 3D printed lattice.

Diaz Perez, Alda / Pysz, Patrick M / Usdrowski, Hunter / Hunter, Victoria K / Stenken, Julie A

Journal of microbiological methods

2022 Volume 204, Page(s) 106644

Abstract: A lattice was designed and fabricated using three-dimensional (3D) printing that allows for the facile transfer of biofilms formed from either Staphylococcus aureus, Staphylococcus epidermidis, or Pseudomonas aeruginosa into a fresh cell culture flask. ... ...

Abstract	A lattice was designed and fabricated using three-dimensional (3D) printing that allows for the facile transfer of biofilms formed from either Staphylococcus aureus, Staphylococcus epidermidis, or Pseudomonas aeruginosa into a fresh cell culture flask. To enhance biofilm production onto the filaments, three protein-based treatments were compared: fetal bovine serum (FBS), bovine serum albumin (BSA), and fibrinogen (Fb). Protein treatments included either supplementing the growth broths or pre-coating the lattice prior to immersion into the broth. S. aureus and P. aeruginosa biofilms were observed on all tested filaments that contained the supplement Fb. S. epidermidis required BSA to form biofilm. Ultimately, polycarbonate (PC) was chosen as the optimal material for lattice creation since it can be autoclaved without warping key design features. In addition, this 3D printed design may facilitate biofilm transfer from the bacterial culture to different cell culture platforms.
MeSH term(s)	Humans ; Pseudomonas aeruginosa ; Staphylococcus aureus ; Staphylococcus epidermidis ; Biofilms ; Staphylococcal Infections/microbiology
Language	English
Publishing date	2022-12-05
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	604916-3
ISSN	1872-8359 ; 0167-7012
ISSN (online)	1872-8359
ISSN	0167-7012
DOI	10.1016/j.mimet.2022.106644
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Article ; Online: Two octave supercontinuum generation in a non-silica graded-index multimode fiber.

Eslami, Zahra / Salmela, Lauri / Filipkowski, Adam / Pysz, Dariusz / Klimczak, Mariusz / Buczynski, Ryszard / Dudley, John M / Genty, Goëry

Nature communications

2022 Volume 13, Issue 1, Page(s) 2126

Abstract: The generation of a two-octave supercontinuum from the visible to mid-infrared (700-2800 nm) in a non-silica graded-index multimode fiber is reported. The fiber design is based on a nanostructured core comprised of two types of drawn lead-bismuth-gallate ...

Abstract	The generation of a two-octave supercontinuum from the visible to mid-infrared (700-2800 nm) in a non-silica graded-index multimode fiber is reported. The fiber design is based on a nanostructured core comprised of two types of drawn lead-bismuth-gallate glass rods with different refractive indices. This yields an effective parabolic index profile and ten times increased nonlinearity when compared to silica fibers. Using femtosecond pulse pumping at wavelengths in both normal and anomalous dispersion regimes, a detailed study is carried out into the supercontinuum generating mechanisms and instabilities seeded by periodic self-imaging. Significantly, suitable injection conditions in the high power regime are found to result in the output beam profile showing clear signatures of beam self-cleaning from nonlinear mode mixing. Experimental observations are interpreted using spatio-temporal 3+1D numerical simulations of the generalized nonlinear Schrödinger equation, and simulated spectra are in excellent agreement with experiment over the full two-octave spectral bandwidth. Experimental comparison with the generation of supercontinuum in a silica graded-index multimode fiber shows that the enhanced nonlinear refractive index of the lead-bismuth-gallate fiber yields a spectrum with a significantly larger bandwidth. These results demonstrate a new pathway towards the generation of bright, ultrabroadband light sources in the mid-infrared.
Language	English
Publishing date	2022-04-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-022-29776-6
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Article: Manufacturing and Recycling Impact on Environmental Life Cycle Assessment of Innovative Wind Power Plant Part 2/2.

Bałdowska-Witos, Patrycja / Doerffer, Krzysztof / Pysz, Michał / Doerffer, Piotr / Tomporowski, Andrzej / Opielak, Marek

Materials (Basel, Switzerland)

2021 Volume 14, Issue 1

Abstract: The process of conversion of wind kinetic energy into electricity in innovative wind power plant emits practically no harmful substances into the environment. However, the production stage of its components requires a lot of energy and materials. The ... ...

Abstract	The process of conversion of wind kinetic energy into electricity in innovative wind power plant emits practically no harmful substances into the environment. However, the production stage of its components requires a lot of energy and materials. The biggest problem during production planning process of an innovative wind power plant is selection of materials and technologies and, consequently, the waste generated at this stage. Therefore, the aim of this publication was to conduct an environmental analysis of the life cycle of elements of a wind turbine by means of life cycle assessment (LCA) method. The object of the research was a wind power plant divided into five sets of components (tower, turbine structure, rotors, generators, and instrumentation), made mainly of steel and small amounts of polymer materials. Eco-indicator 99 was used as an analytical procedure. The impact of the subjects of analysis on human health, ecosystem quality and resources was assessed. Among the analyzed components, the highest level of negative impact on the environment was characterized by the life cycle of the wind turbine tower. The application of recycling processes is reducing the negative impact on the environment in the perspective of the entire life cycle of all studied elements of the wind power plant construction.
Language	English
Publishing date	2021-01-04
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma14010204
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Article: Manufacturing and Recycling Impact on Environmental Life Cycle Assessment of Innovative Wind Power Plant Part 1/2.

Doerffer, Krzysztof / Bałdowska-Witos, Patrycja / Pysz, Michał / Doerffer, Piotr / Tomporowski, Andrzej

Materials (Basel, Switzerland)

2021 Volume 14, Issue 1

Abstract: Wind power plants are considered as an ecologically-clean source of energy. However, manufacturing processes cannot be treated that way. Manufacturing processes consume huge amounts of electrical and thermal energy and significant amount of materials, e ... ...

Abstract	Wind power plants are considered as an ecologically-clean source of energy. However, manufacturing processes cannot be treated that way. Manufacturing processes consume huge amounts of electrical and thermal energy and significant amount of materials, e.g., steel, polymers, oils, and lubricants. All of the above could be potentially harmful for environment. There are not many works and publications regarding life-cycle analysis of wind power plants. This study's objective is to use LCA (Life Cycle Assessment) to the manufacturing and utilization of a specific drag force-driven wind turbine. The discussed innovative wind turbine is of the type that assures safety for prosumer application. Drag force-driven turbines become more heavy than other types of lift driven turbines, but at the same time, their characteristic provides opportunity to use easily recyclable materials instead of materials like plastics or composites. The wider look through LCA tools, may change the perspective of view at that type of wind turbines. Analyzed turbine has capacity of 15 kW and is located in Poland. LCA was carried out using Eco-indicator 99 method in eleven impact categories. Among all of the turbine components, the highest negative impact was noted in the case of the tower. The wind turbine under consideration is characterized by high recycling potential. According to the presented research, recycling provides around 30% reduction of the environmental impact.
Language	English
Publishing date	2021-01-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma14010220
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Article ; Online: Magnetically sensitive fiber probe with nitrogen-vacancy center nanodiamonds integrated in a suspended core.

Filipkowski, Adam / Mrózek, Mariusz / Stępniewski, Grzegorz / Głowacki, Maciej / Pysz, Dariusz / Gawlik, Wojciech / Buczyński, Ryszard / Klimczak, Mariusz / Wojciechowski, Adam

Optics express

2022 Volume 30, Issue 11, Page(s) 19573–19581

Abstract: Efficient collection of photoluminescence arising from spin dynamics of nitrogen vacancy (NV) centers in diamond is important for practical applications involving precise magnetic field or temperature mapping. These goals may be realized by the ... ...

Abstract	Efficient collection of photoluminescence arising from spin dynamics of nitrogen vacancy (NV) centers in diamond is important for practical applications involving precise magnetic field or temperature mapping. These goals may be realized by the integration of nanodiamond particles with optical fibers and volumetric doping of the particles alongside the fiber core. That approach combines the advantages of robust axial fixation of NV diamonds with a direct spatial overlap of their fluorescence with the guided mode of the fiber. We developed a suspended core silicate glass fiber with 750 nm-diameter nanodiamonds located centrally in the 1.5 µm-core cross-section along its axis. The developed fiber probe was tested for its magnetic sensing performance in optically detected magnetic resonance measurements using a 24 cm-long fiber sample, with the NV excitation and fluorescence collection from the far ends of the sample and yielding optical readout contrast of 7% resulting in 0.5 µT·Hz
Language	English
Publishing date	2022-10-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	1491859-6
ISSN	1094-4087 ; 1094-4087
ISSN (online)	1094-4087
ISSN	1094-4087
DOI	10.1364/OE.458162
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Article: Multidisciplinary management of chronic pain in elderly oncology patients.

Pysz-Waberski, Dariusz Tomasz / Sadurska, Joanna / Kędzierska-Jamróz, Joanna / Pietrzyński, Łukasz / Lorek, Andrzej / Jarosz, Magdalena / Gisterek, Iwona

Contemporary oncology (Poznan, Poland)

2022 Volume 26, Issue 3, Page(s) 157–164

Abstract: Chronic pain is one of the most common and most bothersome symptoms in cancer patients, which occurs especially often in the elderly population. Although methods of pain treatment are well known, it is not uncommon for individuals with chronic or ... ...

Abstract	Chronic pain is one of the most common and most bothersome symptoms in cancer patients, which occurs especially often in the elderly population. Although methods of pain treatment are well known, it is not uncommon for individuals with chronic or terminal illnesses to remain underdiagnosed or untreated. Effective pain management has become the measure of success in oncology therapy. For this reason, effective pain management has become an indispensable success factor of multidisciplinary oncological therapy. Along with the growing interest in the holistic approach in medicine, and hence in interdisciplinary treatment, the management of cancer pain in older patients was presented.
Language	English
Publishing date	2022-09-22
Publishing country	Poland
Document type	Journal Article ; Review
ISSN	1428-2526
ISSN	1428-2526
DOI	10.5114/wo.2022.119466
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Article ; Online: All-fiber tunable devices based on high-index photonic crystal fibers filled with liquid crystals.

Ertman, Sławomir / Chychłowski, Miłosz / Bednarska, Karolina / Paździor, Adam / Jaworska, Olga / Czapla, Aleksandra / Bieda, Marcin / Halendy, Monika / Różycka, Julia / Wasilewska, Natalia / Kołodyńska, Oliwia / Harmata, Piotr / Pysz, Dariusz / Buczyński, Ryszard / Woliński, Tomasz R

Optics express

2023 Volume 31, Issue 22, Page(s) 36105–36122

Abstract: In this paper we present all-in fiber tunable devices based on specially designed and optimized high-index photonic crystal fibers filled with nematic liquid crystals. A special host microstructured optical fibers have been designed and manufactured to ... ...

Abstract	In this paper we present all-in fiber tunable devices based on specially designed and optimized high-index photonic crystal fibers filled with nematic liquid crystals. A special host microstructured optical fibers have been designed and manufactured to ensure low-loss index guiding and mode field diameter matching to SMF-28 fiber, ensuring low losses on interconnections with leading in-out FC/PC connectorized pigtails. We present four types of tunable all-fiber devices: tunable retarders with tuning range as high as 20 λ, tunable polarizers with variable axis of polarization and continuously tunable polarization dependent losses, tunable and fully controllable polarization controller and finally indeterministic depolarizer in which depolarization is caused by random thermodynamic process. We also present a cost-effective method to achieve change in the direction of the steering electric field, which was controlled by custom-made programable controllers. Finally, we present a method for effective packaging for the proposed devices.
Language	English
Publishing date	2023-11-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	1491859-6
ISSN	1094-4087 ; 1094-4087
ISSN (online)	1094-4087
ISSN	1094-4087
DOI	10.1364/OE.502351
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Article ; Online: Nanostructured active and photosensitive silica glass for fiber lasers with built-in Bragg gratings.

Franczyk, Marcin / Stefaniuk, Tomasz / Anuszkiewicz, Alicja / Kasztelanic, Rafal / Pysz, Dariusz / Filipkowski, Adam / Osuch, Tomasz / Buczynski, Ryszard

Optics express

2021 Volume 29, Issue 7, Page(s) 10659–10675

Abstract: ... We demonstrated fiber laser with good quality of generated laser beam (M ...

Abstract	A nanostructured core silica fiber with active and photosensitive areas implemented within the fiber core is demonstrated. The photosensitivity, active and passive properties of the fiber can be independently shaped with this new approach. We show that discrete local doping with active ions in form of nanorods allow to obtain effective laser action as in case of continuous distribution of the ions in the core. Co-existing discrete photosensitive nanostructure of germanium doped silica determine single-mode performance and allow inscription of highly efficient Bragg grating over the entire core area. Each nanostructure do not degrade performance of other one since physical interaction between active and photosensitive areas are removed. As a proof of concept, we have designed and fabricated the nanostructured, ytterbium single-mode silica fiber laser with the Bragg grating inscribed in the entire core area. We demonstrated fiber laser with good quality of generated laser beam (M
Language	English
Publishing date	2021-04-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	1491859-6
ISSN	1094-4087 ; 1094-4087
ISSN (online)	1094-4087
ISSN	1094-4087
DOI	10.1364/OE.413433
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