Article: Tyrosine replacement in P-selectin glycoprotein ligand-1 affects distinct kinetic and mechanical properties of bonds with P- and L-selectin.
Proceedings of the National Academy of Sciences of the United States of America
1999 Volume 96, Issue 24, Page(s) 13771–13776
Abstract: ... strength to resist premature dissociation by the forces applied in shear flow. P- and L-selectin bind ... to the N-terminal region of P-selectin glycoprotein ligand-1 (PSGL-1), a mucin on leukocytes. To define ... we compared rolling of transfected preB cells expressing P- or L-selectin on transfected cell monolayers ...
| Abstract | Selectins are adhesion molecules that initiate tethering and rolling of leukocytes on the vessel wall. Rolling requires rapid formation and breakage of selectin-ligand bonds that must have mechanical strength to resist premature dissociation by the forces applied in shear flow. P- and L-selectin bind to the N-terminal region of P-selectin glycoprotein ligand-1 (PSGL-1), a mucin on leukocytes. To define determinants on PSGL-1 that contribute to the kinetic and mechanical properties of bonds with selectins, we compared rolling of transfected preB cells expressing P- or L-selectin on transfected cell monolayers expressing wild-type PSGL-1 or PSGL-1 constructs with substitutions in targeted N-terminal residues. Rolling through P- or L-selectin required a Thr or Ser at a specific position on PSGL-1, the attachment site for an essential O-glycan, but required only one of three nearby Tyr residues, which are sites for Tyr-SO(3) formation. The adhesive strengths and numbers of cells rolling through P- or L-selectin were similar on wild-type PSGL-1 and on each of the three PSGL-1 constructs containing only a single Tyr. However, the cells rolled more irregularly on the single-Tyr forms of PSGL-1. Analysis of the lifetimes of transient tethers on limiting densities of PSGL-1 revealed that L-selectin dissociated faster from single-Tyr than wild-type PSGL-1 at all shears examined. In sharp contrast, P-selectin dissociated faster from single-Tyr than wild-type PSGL-1 at higher shear but not at lower shear. Thus, tyrosine replacements in PSGL-1 affect distinct kinetic and mechanical properties of bonds with P- and L-selectin. |
|---|---|
| MeSH term(s) | Amino Acid Sequence ; Animals ; B-Lymphocytes/cytology ; B-Lymphocytes/metabolism ; CHO Cells ; Cricetinae ; Gene Expression ; Genetic Engineering ; Hematopoietic Stem Cells/cytology ; Hematopoietic Stem Cells/metabolism ; Humans ; Kinetics ; L-Selectin/genetics ; L-Selectin/metabolism ; Ligands ; Membrane Glycoproteins/genetics ; Membrane Glycoproteins/metabolism ; Molecular Sequence Data ; Mucins/genetics ; Mucins/metabolism ; Mutagenesis, Site-Directed ; P-Selectin/genetics ; P-Selectin/metabolism ; Sequence Homology, Amino Acid ; Tyrosine/genetics ; Tyrosine/metabolism |
| Chemical Substances | Ligands ; Membrane Glycoproteins ; Mucins ; P-Selectin ; P-selectin ligand protein ; L-Selectin (126880-86-2) ; Tyrosine (42HK56048U) |
| Language | English |
| Publishing date | 1999-11-23 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S. |
| ZDB-ID | 209104-5 |
| ISSN | 1091-6490 ; 0027-8424 |
| ISSN (online) | 1091-6490 |
| ISSN | 0027-8424 |
| DOI | 10.1073/pnas.96.24.13771 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 1148: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.