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  1. Article ; Online: A dedicated cytoplasmic container collects extrachromosomal DNA away from the mammalian nucleus.

    Schenkel, Laura / Wang, Xuan / Le, Nhung / Burger, Michael / Kroschewski, Ruth

    Molecular biology of the cell

    2023  Volume 34, Issue 11, Page(s) ar105

    Abstract: Expression from transfected plasmid DNA is generally transient, but it is unclear what process terminates it. We show that DNA entering mammalian cells is rapidly surrounded by a double membrane in the cytoplasm, in some cases after leaving the nucleus. ... ...

    Abstract Expression from transfected plasmid DNA is generally transient, but it is unclear what process terminates it. We show that DNA entering mammalian cells is rapidly surrounded by a double membrane in the cytoplasm, in some cases after leaving the nucleus. This cytoplasmic container, termed exclusome, frequently also contains extrachromosomal telomeric DNA, and is maintained by the cell over several division cycles. The exclusome envelope contains endoplasmic reticulum proteins and the inner-nuclear membrane proteins Lap2β and Emerin, but differs from the nuclear envelope by its fenestrations and the absence of the Lamin B Receptor and nuclear pore complexes. Reduction of exclusome frequency upon overexpressing Emerin's LEM-domain suggests a role for Emerin in plasmid DNA compartmentalization. Thus, cells distinguish extrachromosomal DNA and chromosomes and wrap them into similar yet distinct envelopes keeping the former in the exclusome but the latter in the nucleus, where transcription occurs.
    MeSH term(s) Animals ; Nuclear Envelope/metabolism ; Cell Nucleus/metabolism ; Nuclear Pore ; Cytoplasm ; Chromosomes ; Mammals
    Language English
    Publishing date 2023-08-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1098979-1
    ISSN 1939-4586 ; 1059-1524
    ISSN (online) 1939-4586
    ISSN 1059-1524
    DOI 10.1091/mbc.E23-04-0118
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 2853: Show issues Location:
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    Zs.MO 410: Show issues

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  2. Book ; Thesis: Verteilung und subzelluläre Lokalisation von myr 1 und myr 4, zwei Myosinen der Klasse I aus Ratte

    Kroschewski, Ruth

    1995  

    Author's details vorgelegt von Ruth Kroschewski
    Language German
    Size III, 115 S. : Ill., graph. Darst.
    Publisher Kroschewski
    Publishing place Tübingen
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Tübingen, Univ., Diss., 1995
    HBZ-ID HT006800224
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    95 D 3993 order for loan Magazin

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  3. Article: Molecular mechanisms of epithelial polarity: about shapes, forces, and orientation problems.

    Kroschewski, Ruth

    News in physiological sciences : an international journal of physiology produced jointly by the International Union of Physiological Sciences and the American Physiological Society

    2004  Volume 19, Page(s) 61–66

    Abstract: In a variety of organs, epithelial cells assemble into networks of cysts and tubules. Such structures can be reproduced in vitro. Here the importance of plasma membrane compartmentalization and forces that drive morphogenetic events during cystogenesis ... ...

    Abstract In a variety of organs, epithelial cells assemble into networks of cysts and tubules. Such structures can be reproduced in vitro. Here the importance of plasma membrane compartmentalization and forces that drive morphogenetic events during cystogenesis are discussed.
    MeSH term(s) Animals ; Cell Compartmentation/physiology ; Cell Polarity/physiology ; Epithelial Cells/cytology ; Epithelial Cells/physiology ; Humans
    Language English
    Publishing date 2004-03-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 632842-8
    ISSN 1522-161X ; 0886-1714
    ISSN (online) 1522-161X
    ISSN 0886-1714
    DOI 10.1152/nips.01501.2003
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    Zs.A 2164: Show issues Location:
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  4. Article ; Online: Asymmetric division events promote variability in cell cycle duration in animal cells and Escherichia coli.

    Berge, Ulrich / Bochenek, Daria / Schnabel, Ralf / Wehling, Arne / Schroeder, Timm / Stadler, Tanja / Kroschewski, Ruth

    Nature communications

    2019  Volume 10, Issue 1, Page(s) 1901

    Abstract: Asymmetric cell division is a major mechanism generating cell diversity. As cell cycle duration varies among cells in mammalian tissue culture cells, we asked whether their division asymmetry contributes to this variability. We identify among sibling ... ...

    Abstract Asymmetric cell division is a major mechanism generating cell diversity. As cell cycle duration varies among cells in mammalian tissue culture cells, we asked whether their division asymmetry contributes to this variability. We identify among sibling cells an outlier using hierarchical clustering on cell cycle durations of granddaughter cells obtained by lineage tracking of single histone2B-labelled MDCKs. Remarkably, divisions involving outlier cells are not uniformly distributed in lineages, as shown by permutation tests, but appear to emerge from asymmetric divisions taking place at non-stochastic levels: a parent cell influences with 95% confidence and 0.5% error the unequal partitioning of the cell cycle duration in its two progenies. Upon ninein downregulation, this variability propagation is lost, and outlier frequency and variability in cell cycle durations in lineages is reduced. As external influences are not detectable, we propose that a cell-autonomous process, possibly involved in cell specialisation, determines cell cycle duration variability.
    MeSH term(s) Animals ; Asymmetric Cell Division ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Cell Lineage/genetics ; Cell Tracking/methods ; Cytoskeletal Proteins/genetics ; Cytoskeletal Proteins/metabolism ; Dogs ; Escherichia coli/cytology ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Gene Expression ; Genes, Reporter ; Histones/genetics ; Histones/metabolism ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Madin Darby Canine Kidney Cells ; Models, Biological ; Nuclear Proteins/genetics ; Nuclear Proteins/metabolism ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism ; Time Factors
    Chemical Substances Bacterial Proteins ; Cytoskeletal Proteins ; Histones ; Luminescent Proteins ; Nuclear Proteins ; Recombinant Fusion Proteins ; yellow fluorescent protein, Bacteria
    Language English
    Publishing date 2019-04-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-019-09413-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Asymmetric division events promote variability in cell cycle duration in animal cells and Escherichia coli

    Ulrich Berge / Daria Bochenek / Ralf Schnabel / Arne Wehling / Timm Schroeder / Tanja Stadler / Ruth Kroschewski

    Nature Communications, Vol 10, Iss 1, Pp 1-

    2019  Volume 12

    Abstract: We know that variations in cell cycle duration between cells naturally occur but the mechanisms are largely unknown. Here, using lineage tracking, hierarchical clustering and Monte Carlo methods, the authors show that large differences in granddaughter ... ...

    Abstract We know that variations in cell cycle duration between cells naturally occur but the mechanisms are largely unknown. Here, using lineage tracking, hierarchical clustering and Monte Carlo methods, the authors show that large differences in granddaughter cell cycle duration are driven by asymmetric divisions.
    Keywords Science ; Q
    Language English
    Publishing date 2019-04-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Asymmetric division events promote variability in cell cycle duration in animal cells and Escherichia coli

    Ulrich Berge / Daria Bochenek / Ralf Schnabel / Arne Wehling / Timm Schroeder / Tanja Stadler / Ruth Kroschewski

    Nature Communications, Vol 10, Iss 1, Pp 1-

    2019  Volume 12

    Abstract: We know that variations in cell cycle duration between cells naturally occur but the mechanisms are largely unknown. Here, using lineage tracking, hierarchical clustering and Monte Carlo methods, the authors show that large differences in granddaughter ... ...

    Abstract We know that variations in cell cycle duration between cells naturally occur but the mechanisms are largely unknown. Here, using lineage tracking, hierarchical clustering and Monte Carlo methods, the authors show that large differences in granddaughter cell cycle duration are driven by asymmetric divisions.
    Keywords Science ; Q
    Language English
    Publishing date 2019-04-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: The study of polarisation in single cells using model cell membranes.

    Charnley, Mirren / Kroschewski, Ruth / Textor, Marcus

    Integrative biology : quantitative biosciences from nano to macro

    2012  Volume 4, Issue 9, Page(s) 1059–1071

    Abstract: The apicobasal polarisation of epithelial cells within an epithelium is critical for its function as a selective barrier. Microenvironmental parameters, including cell-matrix and cell-cell interactions, contribute to the initiation and orientation of ... ...

    Abstract The apicobasal polarisation of epithelial cells within an epithelium is critical for its function as a selective barrier. Microenvironmental parameters, including cell-matrix and cell-cell interactions, contribute to the initiation and orientation of this polarity. However, it is often non-trivial to decipher the differential effects of these parameters in a controlled manner using traditional in vitro platforms. A reductionist platform, consisting of E-cadherin coupled onto laterally mobile supported lipid bilayers, was utilised to mimic E-cadherin presentation in the cell membrane. These functionalised bilayers were generated either on flat 2D surfaces or the interior surfaces of round microwells. This platform enabled the study of E-cadherin adhesion and the initiation of polarisation in a controlled environment, where the dimensionality of the microenvironment, type of protein coating and cell shape could be independently studied. A high proportion of single epithelial cells interacted with and clustered cellular E-cadherin in the presence of E-cadherin functionalised bilayers, which was reduced in the presence of integrin-mediated adhesion. The differential response in E-cadherin clustering correlated with the polarisation of E-cadherin and Na,K-ATPase, a reporter for the induction of basolateral polarity. Neither the three-dimensional presentation of E-cadherin nor the cell shape affected E-cadherin clustering or polarisation in single cells. Thus, the mobile presentation of E-cadherin was sufficient to mimic a cell-cell contact and induce basolateral polarisation in single cells.
    MeSH term(s) Animals ; Cadherins/metabolism ; Cell Culture Techniques ; Cell Membrane/enzymology ; Cell Membrane/metabolism ; Cell Membrane/physiology ; Cell Polarity/physiology ; Dogs ; Epithelial Cells/cytology ; Epithelial Cells/enzymology ; Lipid Bilayers/metabolism ; Madin Darby Canine Kidney Cells ; Microscopy, Confocal ; Microscopy, Fluorescence ; Sodium-Potassium-Exchanging ATPase/metabolism
    Chemical Substances Cadherins ; Lipid Bilayers ; Sodium-Potassium-Exchanging ATPase (EC 3.6.3.9)
    Language English
    Publishing date 2012-09
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2480063-6
    ISSN 1757-9708 ; 1757-9694
    ISSN (online) 1757-9708
    ISSN 1757-9694
    DOI 10.1039/c2ib20111a
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  8. Article ; Online: Asymmetric partitioning of transfected DNA during mammalian cell division.

    Wang, Xuan / Le, Nhung / Denoth-Lippuner, Annina / Barral, Yves / Kroschewski, Ruth

    Proceedings of the National Academy of Sciences of the United States of America

    2016  Volume 113, Issue 26, Page(s) 7177–7182

    Abstract: Foreign DNA molecules and chromosomal fragments are generally eliminated from proliferating cells, but we know little about how mammalian cells prevent their propagation. Here, we show that dividing human and canine cells partition transfected plasmid ... ...

    Abstract Foreign DNA molecules and chromosomal fragments are generally eliminated from proliferating cells, but we know little about how mammalian cells prevent their propagation. Here, we show that dividing human and canine cells partition transfected plasmid DNA asymmetrically, preferentially into the daughter cell harboring the young centrosome. Independently of how they entered the cell, most plasmids clustered in the cytoplasm. Unlike polystyrene beads of similar size, these clusters remained relatively immobile and physically associated to endoplasmic reticulum-derived membranes, as revealed by live cell and electron microscopy imaging. At entry of mitosis, most clusters localized near the centrosomes. As the two centrosomes split to assemble the bipolar spindle, predominantly the old centrosome migrated away, biasing the partition of the plasmid cluster toward the young centrosome. Down-regulation of the centrosomal proteins Ninein and adenomatous polyposis coli abolished this bias. Thus, we suggest that DNA clustering, cluster immobilization through association to the endoplasmic reticulum membrane, initial proximity between the cluster and centrosomes, and subsequent differential behavior of the two centrosomes together bias the partition of plasmid DNA during mitosis. This process leads to their progressive elimination from the proliferating population and might apply to any kind of foreign DNA molecule in mammalian cells. Furthermore, the functional difference of the centrosomes might also promote the asymmetric partitioning of other cellular components in other mammalian and possibly stem cells.
    MeSH term(s) Animals ; Cell Division ; Centrosome/metabolism ; Cytoskeletal Proteins/genetics ; DNA/metabolism ; Dogs ; Endoplasmic Reticulum/metabolism ; HeLa Cells ; Humans ; Madin Darby Canine Kidney Cells ; Mitosis ; Nuclear Proteins/genetics ; Plasmids ; Transfection
    Chemical Substances Cytoskeletal Proteins ; NIN protein, human ; Nuclear Proteins ; DNA (9007-49-2)
    Language English
    Publishing date 2016--28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.1606091113
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    Zs.A 1148: Show issues Location:
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  9. Article ; Online: Tissue-culture light sheet fluorescence microscopy (TC-LSFM) allows long-term imaging of three-dimensional cell cultures under controlled conditions.

    Pampaloni, Francesco / Berge, Ulrich / Marmaras, Anastasios / Horvath, Peter / Kroschewski, Ruth / Stelzer, Ernst H K

    Integrative biology : quantitative biosciences from nano to macro

    2014  Volume 6, Issue 10, Page(s) 988–998

    Abstract: Fluorescence long-term imaging of cellular processes in three-dimensional cultures requires the control of media supply, temperature, and pH, as well as minimal photodamage. We describe a system based on a light sheet fluorescence microscope (LSFM), ... ...

    Abstract Fluorescence long-term imaging of cellular processes in three-dimensional cultures requires the control of media supply, temperature, and pH, as well as minimal photodamage. We describe a system based on a light sheet fluorescence microscope (LSFM), which is optimized for long-term, multi-position imaging of three-dimensional in-gel cell cultures. The system integrates a stable culture condition control system in the optical path of the light-sheet microscope. A further essential element is a biocompatible agarose container suitable for the LSFM, in which any cell type can be cultured in different gel matrices. The TC-LSFM allows studying any in vitro cultured cell type reacting to, dividing in, or migrating through a three-dimensional extracellular matrix (ECM) gel. For this reason we called it "tissue culture-LSFM" (TC-LSFM). The TC-LSFM system allows fast imaging at multiple locations within a millimeter-sized ECM gel. This increases the number of analyzed events and allows testing population effects. As an example, we show the maturation of a cyst of MDCK (canine kidney epithelial) cells over a period of three days. Moreover, we imaged, tracked, and analyzed MDCK cells during the first five days of cell aggregate formation and discovered a remarkable heterogeneity in cell cycle lengths and an interesting cell death pattern. Thus, TC-LSFM allows performing new long-term assays assessing cellular behavior in three-dimensional ECM-gel cultures. For example migration, invasion or differentiation in epithelial cell systems, stem cells, as well as cancer cells can be investigated.
    MeSH term(s) Animals ; Cell Culture Techniques/instrumentation ; Cell Culture Techniques/methods ; Cell Line ; Dogs ; Epithelial Cells/cytology ; Epithelial Cells/physiology ; Extracellular Matrix/physiology ; Image Processing, Computer-Assisted ; Imaging, Three-Dimensional/methods ; Microscopy, Fluorescence/instrumentation ; Microscopy, Fluorescence/methods ; Statistics, Nonparametric
    Language English
    Publishing date 2014-10
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2480063-6
    ISSN 1757-9708 ; 1757-9694
    ISSN (online) 1757-9708
    ISSN 1757-9694
    DOI 10.1039/c4ib00121d
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Book ; Thesis: Verteilung und subzelluläre Lokalisation von myr 1 und myr 4, zwei Myosinen der Klasse I aus Ratte

    Kroschewski, Ruth

    1995  

    Author's details vorgelegt von Ruth Kroschewski
    Language German
    Size III, 115 S, Ill., graph. Darst, 21 cm
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Univ., Diss.--Tübingen, 1995
    Database Former special subject collection: coastal and deep sea fishing

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