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  1. Article ; Online: Interaction of Mycobacteria With Host Cell Inflammasomes.

    Rastogi, Shivangi / Briken, Volker

    Frontiers in immunology

    2022  Volume 13, Page(s) 791136

    Abstract: The inflammasome complex is important for host defense against intracellular bacterial infections. ...

    Abstract The inflammasome complex is important for host defense against intracellular bacterial infections.
    MeSH term(s) Humans ; Inflammasomes/metabolism ; Interleukin-1beta/metabolism ; Macrophages/metabolism ; Mycobacterium Infections/metabolism ; Mycobacterium tuberculosis ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
    Chemical Substances Inflammasomes ; Interleukin-1beta ; NLR Family, Pyrin Domain-Containing 3 Protein
    Language English
    Publishing date 2022-02-14
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2022.791136
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Salmonella

    Shuster, Michael / Lyu, Zhihui / Augenstreich, Jacques / Mathur, Shrestha / Ganesh, Akshaya / Ling, Jiqiang / Briken, Volker

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Type I Interferons (IFNs) generally have a protective role during viral infections, but their function during bacterial infections is dependent on the bacterial species. ...

    Abstract Type I Interferons (IFNs) generally have a protective role during viral infections, but their function during bacterial infections is dependent on the bacterial species.
    Language English
    Publishing date 2024-03-05
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.03.05.583530
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: BBQ methods: streamlined workflows for bacterial burden quantification in infected cells by confocal microscopy.

    Augenstreich, Jacques / Shuster, Michael / Fan, Yongqiang / Lyu, Zhihui / Ling, Jiqiang / Briken, Volker

    Biology open

    2024  Volume 13, Issue 1

    Abstract: Accurate quantification of bacterial burden within macrophages, termed bacterial burden quantification (BBQ), is crucial for understanding host-pathogen interactions. Various methods have been employed, each with strengths and weaknesses. This article ... ...

    Abstract Accurate quantification of bacterial burden within macrophages, termed bacterial burden quantification (BBQ), is crucial for understanding host-pathogen interactions. Various methods have been employed, each with strengths and weaknesses. This article addresses limitations in existing techniques and introduces two novel, automated methods for BBQ within macrophages based on confocal microscopy data analysis. The first method refines total fluorescence quantification by incorporating filtering steps to exclude uninfected cells, while the second method calculates total bacterial volume per cell to mitigate potential biases in fluorescence-based readouts. These workflows utilize PyImageJ and Cellpose software, providing reliable, unbiased, and rapid quantification of bacterial load. The proposed workflows were validated using Salmonella enterica serovar Typhimurium and Mycobacterium tuberculosis models, demonstrating their effectiveness in accurately assessing bacterial burden. These automated workflows offer valuable tools for studying bacterial interactions within host cells and provide insights for various research applications.
    MeSH term(s) Workflow ; Salmonella typhimurium ; Macrophages ; Host-Pathogen Interactions
    Language English
    Publishing date 2024-01-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 2632264-X
    ISSN 2046-6390 ; 2046-6390
    ISSN (online) 2046-6390
    ISSN 2046-6390
    DOI 10.1242/bio.060189
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: da_Tracker: Automated workflow for high throughput single cell and single phagosome tracking in infected cells.

    Augenstreich, Jacques / Poddar, Anushka / Belew, Ashton T / El-Sayed, Najib M / Briken, Volker

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Time-lapse microscopy has emerged as a crucial tool in cell biology, facilitating a deeper understanding of dynamic cellular processes. While existing tracking tools have proven effective in detecting and monitoring objects over time, the quantification ... ...

    Abstract Time-lapse microscopy has emerged as a crucial tool in cell biology, facilitating a deeper understanding of dynamic cellular processes. While existing tracking tools have proven effective in detecting and monitoring objects over time, the quantification of signals within these tracked objects often faces implementation constraints. In the context of infectious diseases, the quantification of signals at localized compartments within the cell and around intracellular pathogens can provide even deeper insight into the interactions between the pathogen and host cell organelles. Existing quantitative analysis at a single-phagosome level remains limited and dependent on manual tracking methods. We developed a near-fully automated workflow that performs with limited bias, high-throughput cell segmentation and quantitative tracking of both single cell and single bacterium/phagosome within multi-channel, z-stack, time-lapse confocal microscopy videos. We took advantage of the PyImageJ library to bring Fiji functionality into a Python environment and combined deep-learning-based segmentation from Cellpose with tracking algorithms from Trackmate. Our workflow provides a versatile toolkit of functions for measuring relevant signal parameters at the single-cell level (such as velocity or bacterial burden) and at the single-phagosome level (
    Language English
    Publishing date 2024-04-11
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.04.10.588863
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Putting the p(hosphor) in pyroptosis.

    Rastogi, Shivangi / Evavold, Charles L / Briken, Volker

    Cell host & microbe

    2022  Volume 30, Issue 12, Page(s) 1650–1652

    Abstract: A recent study in Science found Mycobacterium tuberculosis inhibits pyroptosis of the host cell by secreting a phosphatase (PtpB). PtpB targets the plasma membrane to dephosphorylate PI4P and PI(4,5) ... ...

    Abstract A recent study in Science found Mycobacterium tuberculosis inhibits pyroptosis of the host cell by secreting a phosphatase (PtpB). PtpB targets the plasma membrane to dephosphorylate PI4P and PI(4,5)P
    MeSH term(s) Mice ; Animals ; Pyroptosis ; Phosphate-Binding Proteins/metabolism ; Intracellular Signaling Peptides and Proteins/metabolism ; Cell Membrane/metabolism ; Mycobacterium tuberculosis/metabolism
    Chemical Substances Phosphate-Binding Proteins ; Intracellular Signaling Peptides and Proteins
    Language English
    Publishing date 2022-12-16
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2278004-X
    ISSN 1934-6069 ; 1931-3128
    ISSN (online) 1934-6069
    ISSN 1931-3128
    DOI 10.1016/j.chom.2022.11.010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6578: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Host Cell Targets of Released Lipid and Secreted Protein Effectors of

    Augenstreich, Jacques / Briken, Volker

    Frontiers in cellular and infection microbiology

    2020  Volume 10, Page(s) 595029

    Abstract: Mycobacterium ... ...

    Abstract Mycobacterium tuberculosis
    MeSH term(s) Bacterial Proteins ; Humans ; Lipids ; Macrophages ; Mycobacterium tuberculosis ; Phagosomes
    Chemical Substances Bacterial Proteins ; Lipids
    Language English
    Publishing date 2020-10-23
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2020.595029
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  7. Article: BBQ methods: Streamlined workflows for Bacterial Burden Quantification in infected cells by confocal microscopy.

    Augenstreich, Jacques / Shuster, Michael / Fan, Yongqiang / Lyu, Zhihui / Ling, Jiqiang / Briken, Volker

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Accurate quantification of bacterial burden within macrophages, termed Bacterial Burden Quantification (BBQ), is crucial for understanding host-pathogen interactions. Various methods have been employed, each with strengths and weaknesses. This article ... ...

    Abstract Accurate quantification of bacterial burden within macrophages, termed Bacterial Burden Quantification (BBQ), is crucial for understanding host-pathogen interactions. Various methods have been employed, each with strengths and weaknesses. This article addresses limitations in existing techniques and introduces two novel automated methods for BBQ within macrophages based on confocal microscopy data analysis. The first method refines total fluorescence quantification by incorporating filtering steps to exclude uninfected cells, while the second method calculates total bacterial volume per cell to mitigate potential biases in fluorescence-based readouts. These workflows utilize PyImageJ and Cellpose software, providing reliable, unbiased, and rapid quantification of bacterial load. The proposed workflows were validated using Salmonella enterica serovar Typhimurium and Mycobacterium tuberculosis models, demonstrating their effectiveness in accurately assessing bacterial burden. These automated workflows offer valuable tools for studying bacterial interactions within host cells and provide insights for various research applications.
    Language English
    Publishing date 2023-10-02
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.10.01.560379
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Apoptosis inhibition by intracellular bacteria and its consequence on host immunity.

    Behar, Samuel M / Briken, Volker

    Current opinion in immunology

    2019  Volume 60, Page(s) 103–110

    Abstract: Regulated cell death via apoptosis not only is important for organismal homeostasis but also serves as an innate defense mechanism. The engulfment of apoptotic infected cells, a process known as efferocytosis, is a common pathway for the destruction of ... ...

    Abstract Regulated cell death via apoptosis not only is important for organismal homeostasis but also serves as an innate defense mechanism. The engulfment of apoptotic infected cells, a process known as efferocytosis, is a common pathway for the destruction of many intracellular bacteria. Some pathogens take advantage of efferocytosis to prevent activation of macrophages and thereby facilitate their dissemination. Conversely, many obligate intracellular bacterial pathogens and some facultative-intracellular bacteria inhibit apoptosis, preventing efferocytosis, and evading innate host defenses. The molecular mechanism of bacterial effectors includes secreted proteins that bind to and inhibit apoptosis cell signaling pathways. We provide an overview of the known bacterial effectors, their host cell targets and their importance for the virulence of human pathogens.
    MeSH term(s) Animals ; Apoptosis/immunology ; Bacterial Infections/immunology ; Bacterial Infections/metabolism ; Bacterial Infections/microbiology ; Bacterial Physiological Phenomena ; Biomarkers ; Host-Pathogen Interactions/immunology ; Humans ; Immunity ; Immunity, Innate ; Intracellular Space/immunology ; Intracellular Space/metabolism ; Intracellular Space/microbiology ; Phagocytosis/immunology ; Signal Transduction
    Chemical Substances Biomarkers
    Language English
    Publishing date 2019-06-19
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 1035767-1
    ISSN 1879-0372 ; 0952-7915
    ISSN (online) 1879-0372
    ISSN 0952-7915
    DOI 10.1016/j.coi.2019.05.007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2773: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 13: Show issues

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  9. Article: Mycobacterium tuberculosis genes involved in regulation of host cell death.

    Briken, Volker

    Advances in experimental medicine and biology

    2013  Volume 783, Page(s) 93–102

    Abstract: The topic of host cell death response upon Mycobacterium tuberculosis (Mtb) infection has been a controversial one [1]. Recent findings demonstrate that one of the important confounding factors was most likely the fact that while Mtb inhibits host cell ... ...

    Abstract The topic of host cell death response upon Mycobacterium tuberculosis (Mtb) infection has been a controversial one [1]. Recent findings demonstrate that one of the important confounding factors was most likely the fact that while Mtb inhibits host cell apoptosis induction early during the infection it clearly induces a necrotic form of cell death during later infection stages [2, 3]. This bi-phasic intracellular lifestyle in regard to host cell death manipulation is emerging as a common theme shared with other facultative and obligate intracellular bacterial pathogens such as Chlamydia and Legionella [4-6]. Accordingly, the list of discovered bacterial proteins involved in host cell apoptosis inhibition is growing [7, 8]. At the same time it is clearly beneficial for the resistance of the host to overcome the bacterial apoptosis block during the early stage of the infection [9-11]. Hence, host cell components have evolved to recognize intracellular pathogens and mediate host cell apoptosis induction if necessary [12]. There have been several reviews on the various aspects of the host cell death response upon Mtb infection [1, 3, 13-15]. Thus in this chapter I will focus on the pathogen side of the equation and describe the tremendous progress that has been made in the identification and characterization of Mtb genes involved in manipulation of host cell death pathways.
    MeSH term(s) Animals ; Apoptosis/genetics ; Bacterial Proteins/genetics ; Bacterial Proteins/physiology ; Genes, Bacterial ; Humans ; Immune Evasion/genetics ; Macrophages/immunology ; Macrophages/microbiology ; Macrophages/pathology ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/physiology ; Necrosis/genetics ; Phagosomes/physiology ; Reactive Nitrogen Species ; Reactive Oxygen Species ; Tuberculosis/immunology ; Tuberculosis/microbiology ; Tuberculosis/pathology
    Chemical Substances Bacterial Proteins ; Reactive Nitrogen Species ; Reactive Oxygen Species
    Language English
    Publishing date 2013
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ISSN 2214-8019 ; 0065-2598
    ISSN (online) 2214-8019
    ISSN 0065-2598
    DOI 10.1007/978-1-4614-6111-1_5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Legionella pneumophila

    Allen, Charles N S / Banks, Dallas A / Shuster, Michael / Vogel, Stefanie N / O'Connor, Tamara J / Briken, Volker

    Infection and immunity

    2023  Volume 91, Issue 11, Page(s) e0036523

    Abstract: The host type I interferon (IFN) response protects ... ...

    Abstract The host type I interferon (IFN) response protects against
    MeSH term(s) Humans ; Legionella pneumophila/physiology ; Type IV Secretion Systems ; Interferon-gamma/metabolism ; Legionnaires' Disease ; Signal Transduction ; Interferon Type I
    Chemical Substances Type IV Secretion Systems ; Interferon-gamma (82115-62-6) ; Interferon Type I
    Language English
    Publishing date 2023-10-16
    Publishing country United States
    Document type Journal Article
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/iai.00365-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 684: Show issues Location:
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