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Article ; Online: Current Insights in Elucidation of Possible Molecular Mechanisms of the Juvenile Form of Batten Disease.

Shematorova, Elena K / Shpakovski, George V

International journal of molecular sciences

2020 Volume 21, Issue 21

Abstract: The neuronal ceroid lipofuscinoses (NCLs) collectively constitute one of the most common forms of inherited childhood-onset neurodegenerative disorders. They form a heterogeneous group of incurable lysosomal storage diseases that lead to blindness, motor ...

Abstract	The neuronal ceroid lipofuscinoses (NCLs) collectively constitute one of the most common forms of inherited childhood-onset neurodegenerative disorders. They form a heterogeneous group of incurable lysosomal storage diseases that lead to blindness, motor deterioration, epilepsy, and dementia. Traditionally the NCL diseases were classified according to the age of disease onset (infantile, late-infantile, juvenile, and adult forms), with at least 13 different NCL varieties having been described at present. The current review focuses on classic juvenile NCL (JNCL) or the so-called Batten (Batten-Spielmeyer-Vogt; Spielmeyer-Sjogren) disease, which represents the most common and the most studied form of NCL, and is caused by mutations in the
MeSH term(s)	Animals ; Child ; Humans ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Molecular Chaperones/metabolism ; Mutation ; Neuronal Ceroid-Lipofuscinoses/physiopathology
Chemical Substances	Membrane Proteins ; Molecular Chaperones
Language	English
Publishing date	2020-10-29
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms21218055
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: The Human Isoform of RNA Polymerase II Subunit hRPB11bα Specifically Interacts with Transcription Factor ATF4.

Proshkin, Sergey A / Shematorova, Elena K / Shpakovski, George V

International journal of molecular sciences

2019 Volume 21, Issue 1

Abstract: Rpb11 subunit of RNA polymerase II of Eukaryotes is related to N-terminal domain of eubacterial α subunit and forms a complex with Rpb3 subunit analogous to prokaryotic ... ...

Abstract	Rpb11 subunit of RNA polymerase II of Eukaryotes is related to N-terminal domain of eubacterial α subunit and forms a complex with Rpb3 subunit analogous to prokaryotic α
MeSH term(s)	Activating Transcription Factor 4/genetics ; Activating Transcription Factor 4/metabolism ; Humans ; Protein Binding ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; RNA Polymerase II/genetics ; RNA Polymerase II/metabolism ; Two-Hybrid System Techniques
Chemical Substances	Protein Isoforms ; Activating Transcription Factor 4 (145891-90-3) ; POLR2J protein, human (EC 2.7.7.-) ; RNA Polymerase II (EC 2.7.7.-)
Language	English
Publishing date	2019-12-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms21010135
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Article ; Online: Current Insights in Elucidation of Possible Molecular Mechanisms of the Juvenile Form of Batten Disease

Elena K. Shematorova / George V. Shpakovski

International Journal of Molecular Sciences, Vol 21, Iss 8055, p

2020 Volume 8055

Abstract	The neuronal ceroid lipofuscinoses (NCLs) collectively constitute one of the most common forms of inherited childhood-onset neurodegenerative disorders. They form a heterogeneous group of incurable lysosomal storage diseases that lead to blindness, motor deterioration, epilepsy, and dementia. Traditionally the NCL diseases were classified according to the age of disease onset (infantile, late-infantile, juvenile, and adult forms), with at least 13 different NCL varieties having been described at present. The current review focuses on classic juvenile NCL (JNCL) or the so-called Batten (Batten-Spielmeyer-Vogt; Spielmeyer-Sjogren) disease, which represents the most common and the most studied form of NCL, and is caused by mutations in the CLN3 gene located on human chromosome 16. Most JNCL patients carry the same 1.02-kb deletion in this gene, encoding an unusual transmembrane protein, CLN3, or battenin. Accordingly, the names CLN3 -related neuronal ceroid lipofuscinosis or CLN3 -disease sometimes have been used for this malady. Despite excessive in vitro and in vivo studies, the precise functions of the CLN3 protein and the JNCL disease mechanisms remain elusive and are the main subject of this review. Although the CLN3 gene is highly conserved in evolution of all mammalian species, detailed analysis of recent genomic and transcriptomic data indicates the presence of human-specific features of its expression, which are also under discussion. The main recorded to date changes in cell metabolism, to some extent contributing to the emergence and progression of JNCL disease, and human-specific molecular features of CLN3 gene expression are summarized and critically discussed with an emphasis on the possible molecular mechanisms of the malady appearance and progression.
Keywords	neuronal ceroid lipofuscinoses ; juvenile Batten disease ; JNCL ; CLN3 ; CLN3 gene regulation ; biomarker POLR2J2 ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	610
Language	English
Publishing date	2020-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Molecular mechanisms of the juvenile form of Batten disease: important role of MAPK signaling pathways (ERK1/ERK2, JNK and p38) in pathogenesis of the malady.

Shematorova, Elena K / Shpakovski, Dmitry G / Chernysheva, Anna D / Shpakovski, George V

Biology direct

2018 Volume 13, Issue 1, Page(s) 19

Abstract: ... Particularly, CLN3 interacts with a subunit of Na/K ATPase ATP1A1 which changes its conformation and activates ...

Abstract	Background: Mutations in the CLN3 gene lead to so far an incurable juvenile-onset neuronal ceroid lipofuscinosis (JNCL) or Batten disease that starts at the age of 4-6 years with a progressive retinopathy leading to blindness. Motor disturbances, epilepsy and dementia manifest during several following years. Most JNCL patients carry the same 1.02-kb deletion in the CLN3 gene, encoding an unusual transmembrane protein, CLN3 or battenin. Results: Based on data of genome-wide expression profiling in CLN3 patients with different rate of the disease progression [Mol. Med., 2011, 17: 1253-1261] and our bioinformatic analysis of battenin protein-protein interactions in neurons we propose that CLN3 can function as a molecular chaperone for some plasma membrane proteins, being crucially important for their correct folding in endoplasmic reticulum. Changes in spatial structure of these membrane proteins lead to transactivation of the located nearby receptors. Particularly, CLN3 interacts with a subunit of Na/K ATPase ATP1A1 which changes its conformation and activates the adjacent epidermal growth factor receptor (EGFR). As a result, a large amount of erroneously activated EGFR generates MAPK signal cascades (ERK1/ERK2, JNKs and p38) from cell surface eventually causing neurons' death. Conclusions: Molecular mechanism of the juvenile form of Batten disease (JNCL), which is based on the excessive activation of signaling cascades in a time of the radical increase of neuronal membranes' area in the growing brain, have been proposed and substantiated. The primary cause of this phenomenon is the defective function of the CLN3 protein that could not act properly as molecular chaperone for some plasma membrane proteins in the endoplasmic reticulum. The incorrect three-dimensional structure of at least one such protein, ATP1A1, leads to unregulated spontaneous and repetitive activation of the SRC kinase that transactivates EGFR with the subsequent uncontrolled launch of various MAPK cascades. Possible ways of treatment of patients with JNCL have been suggested. Reviewers: This article was reviewed by Konstantinos Lefkimmiatis, Eugene Koonin and Vladimir Poroikov.
MeSH term(s)	Computational Biology ; Disease Progression ; Genome-Wide Association Study ; Humans ; Membrane Glycoproteins/genetics ; Membrane Glycoproteins/metabolism ; Mitogen-Activated Protein Kinase 1/genetics ; Mitogen-Activated Protein Kinase 1/metabolism ; Molecular Chaperones/genetics ; Molecular Chaperones/metabolism ; Neuronal Ceroid-Lipofuscinoses/genetics ; Signal Transduction/genetics
Chemical Substances	CLN3 protein, human ; Membrane Glycoproteins ; Molecular Chaperones ; MAPK1 protein, human (EC 2.7.11.24) ; Mitogen-Activated Protein Kinase 1 (EC 2.7.11.24)
Language	English
Publishing date	2018-09-25
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1745-6150
ISSN (online)	1745-6150
DOI	10.1186/s13062-018-0212-y
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Article ; Online: The Human Isoform of RNA Polymerase II Subunit hRPB11bα Specifically Interacts with Transcription Factor ATF4

Sergey A. Proshkin / Elena K. Shematorova / George V. Shpakovski

International Journal of Molecular Sciences, Vol 21, Iss 1, p

2019 Volume 135

Abstract: Rpb11 subunit of RNA polymerase II of Eukaryotes is related to N-terminal domain of eubacterial α subunit and forms a complex with Rpb3 subunit analogous to prokaryotic α 2 homodimer, which is involved in RNA polymerase assembly and promoter recognition. ...

Abstract	Rpb11 subunit of RNA polymerase II of Eukaryotes is related to N-terminal domain of eubacterial α subunit and forms a complex with Rpb3 subunit analogous to prokaryotic α 2 homodimer, which is involved in RNA polymerase assembly and promoter recognition. In humans, a POLR2J gene family has been identified that potentially encodes several hRPB11 proteins differing mainly in their short C-terminal regions. The functions of the different human specific isoforms are still mainly unknown. To further characterize the minor human specific isoform of RNA polymerase II subunit hRPB11bα, the only one from hRPB11 (POLR2J) homologues that can replace its yeast counterpart in vivo, we used it as bait in a yeast two-hybrid screening of a human fetal brain cDNA library. By this analysis and subsequent co-purification assay in vitro, we identified transcription factor ATF4 as a prominent partner of the minor RNA polymerase II (RNAP II) subunit hRPB11bα. We demonstrated that the hRPB11bα interacts with leucine b-Zip domain located on the C-terminal part of ATF4. Overexpression of ATF4 activated the reporter more than 10-fold whereas co-transfection of hRPB11bα resulted in a 2.5-fold enhancement of ATF4 activation. Our data indicate that the mode of interaction of human RNAP II main (containing major for of hRPB11 subunit) and minor (containing hRPB11bα isoform of POLR2J subunit) transcription enzymes with ATF4 is certainly different in the two complexes involving hRPB3−ATF4 (not hRPB11a−ATF4) and hRpb11bα−ATF4 platforms in the first and the second case, respectively. The interaction of hRPB11bα and ATF4 appears to be necessary for the activation of RNA polymerase II containing the minor isoform of the hRPB11 subunit (POLR2J) on gene promoters regulated by this transcription factor. ATF4 activates transcription by directly contacting RNA polymerase II in the region of the heterodimer of α-like subunits (Rpb3−Rpb11) without involving a Mediator, which provides fast and highly effective activation of transcription of the desired ...
Keywords	rna polymerase ii ; human isoforms of rpb11 ; hrpb11bα ; hrpb11a ; atf4 ; yeast two-hybrid system ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	572
Language	English
Publishing date	2019-12-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Molecular mechanisms of the juvenile form of Batten disease

Elena K. Shematorova / Dmitry G. Shpakovski / Anna D. Chernysheva / George V. Shpakovski

Biology Direct, Vol 13, Iss 1, Pp 1-

important role of MAPK signaling pathways (ERK1/ERK2, JNK and p38) in pathogenesis of the malady

2018 Volume 9

Abstract: ... Particularly, CLN3 interacts with a subunit of Na/K ATPase ATP1A1 which changes its conformation and activates ...

Abstract	Abstract Background Mutations in the CLN3 gene lead to so far an incurable juvenile-onset neuronal ceroid lipofuscinosis (JNCL) or Batten disease that starts at the age of 4–6 years with a progressive retinopathy leading to blindness. Motor disturbances, epilepsy and dementia manifest during several following years. Most JNCL patients carry the same 1.02-kb deletion in the CLN3 gene, encoding an unusual transmembrane protein, CLN3 or battenin. Results Based on data of genome-wide expression profiling in CLN3 patients with different rate of the disease progression [Mol. Med., 2011, 17: 1253–1261] and our bioinformatic analysis of battenin protein-protein interactions in neurons we propose that CLN3 can function as a molecular chaperone for some plasma membrane proteins, being crucially important for their correct folding in endoplasmic reticulum. Changes in spatial structure of these membrane proteins lead to transactivation of the located nearby receptors. Particularly, CLN3 interacts with a subunit of Na/K ATPase ATP1A1 which changes its conformation and activates the adjacent epidermal growth factor receptor (EGFR). As a result, a large amount of erroneously activated EGFR generates MAPK signal cascades (ERK1/ERK2, JNKs and p38) from cell surface eventually causing neurons’ death. Conclusions Molecular mechanism of the juvenile form of Batten disease (JNCL), which is based on the excessive activation of signaling cascades in a time of the radical increase of neuronal membranes’ area in the growing brain, have been proposed and substantiated. The primary cause of this phenomenon is the defective function of the CLN3 protein that could not act properly as molecular chaperone for some plasma membrane proteins in the endoplasmic reticulum. The incorrect three-dimensional structure of at least one such protein, ATP1A1, leads to unregulated spontaneous and repetitive activation of the SRC kinase that transactivates EGFR with the subsequent uncontrolled launch of various MAPK cascades. Possible ways of treatment of ...
Keywords	Juvenile neuronal ceroid lipofuscinosis (Batten disease) ; CLN3 ; Molecular chaperone ; ATP1A1 ; SRC ; EGFR ; Biology (General) ; QH301-705.5
Subject code	612
Language	English
Publishing date	2018-09-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Novel Interactions of Adrenodoxin-Related [2Fe-2S] Plant Ferredoxins MFDX1 and MFDX2 Indicate Their Involvement in a Wide Spectrum of Functions in Plant Mitochondria

Shematorova, Elena K / Slovokhotov, Ivan Yu / Shmakov, Vladimir N / Khaliluev, Marat R / Shpakovski, Dmitry G / Klykov, Valery N / Babak, Olga G / Spivak, Svetlana G / Konstantinov, Yuri M / Shpakovski, George V

Proceedings of the Latvian Academy of Sciences. 2019 Dec. 01, v. 73, no. 6

2019

Abstract: Electron transfer chains of plant organelles (both chloroplasts and mitochondria) contain their own special set of ferredoxins. The relatively recently described adrenodoxin-like [2Fe-2S]-ferredoxins MFDX1 and MFDX2 of plant mitochondria are among the ... ...

Abstract	Electron transfer chains of plant organelles (both chloroplasts and mitochondria) contain their own special set of ferredoxins. The relatively recently described adrenodoxin-like [2Fe-2S]-ferredoxins MFDX1 and MFDX2 of plant mitochondria are among the least studied of these. Until now, the only established function for them is participation in the final stage of biotin biosynthesis. In this work, using genetic and biochemical approaches, we searched for possible partners of these proteins in the genomes and proteomes of tobacco (Nicotiana tabacum L.) and foxglove (Digitalis purpurea L.) plants. MORF9 protein, one of the auxiliary components of the RNA editing complex of organelles (editosome), was found among the most prominent protein partners of adrenodoxin-like [2Fe-2S] tobacco ferredoxins. According to the results obtained from the yeast two-hybrid system, NtMFDX1 and NtMFDX2 of tobacco also bind and interact productively with the previously uncharacterised long non-coding polyadenylated RNA, which, based on its structural features, is capable of regulating the function of a number of components of complexes I (Nad1, Nad5) and III (protein of the cytochrome c synthesis system CcmF) and contributes to the formation of Fe/S-clusters in the corresponding protein complexes of the respiratory chain of plant mitochondria. We found one of the main components of the thiazol synthase complex (mitochondrial protein DpTHI1) to be the partner of ferredoxin DpMFDX2 of Digitalis purpurea. Finally, additional arguments were obtained in favour of the possible participation of MFDX1 and MFDX2 in the very ancient, but only recently described ‘progesterone’ steroid hormonal regulatory system: in leaves of the previously constructed CYP11A1-transgenic tomato plants, only the mature form of mitochondrial cytochrome P450scc (CYP11A1) of mammals is able to enter the mitochondria, where the above-mentioned components of the electron transport chain are localised. In summary, all of the newly revealed interactions of adrenodoxin-like [2Fe-2S] ferredoxins MFDX1 and MFDX2 indicate their participation in a wide range of functions in plant mitochondria.
Keywords	Digitalis purpurea ; Nicotiana tabacum ; biosynthesis ; biotin ; chloroplasts ; electron transfer ; electron transport chain ; ferredoxins ; genome ; messenger RNA ; mitochondria ; mitochondrial proteins ; proteome ; tobacco ; tomatoes ; two hybrid system techniques
Language	English
Dates of publication	2019-1201
Size	p. 478-486.
Publishing place	Sciendo
Document type	Article
Note	NAL-AP-2-clean
ISSN	2255-890X
DOI	10.2478/prolas-2019-0074
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Novel Interactions of Adrenodoxin-Related [2Fe-2S] Plant Ferredoxins MFDX1 and MFDX2 Indicate Their Involvement in a Wide Spectrum of Functions in Plant Mitochondria

Shematorova Elena K. / Slovokhotov Ivan Yu. / Shmakov Vladimir N. / Khaliluev Marat R. / Shpakovski Dmitry G. / Klykov Valery N. / Babak Olga G. / Spivak Svetlana G. / Konstantinov Yuri M. / Shpakovski George V.

Proceedings of the Latvian Academy of Sciences. Section B, Natural Sciences, Vol 73, Iss 6, Pp 478-

2019 Volume 486

Abstract	Electron transfer chains of plant organelles (both chloroplasts and mitochondria) contain their own special set of ferredoxins. The relatively recently described adrenodoxin-like [2Fe-2S]-ferredoxins MFDX1 and MFDX2 of plant mitochondria are among the least studied of these. Until now, the only established function for them is participation in the final stage of biotin biosynthesis. In this work, using genetic and biochemical approaches, we searched for possible partners of these proteins in the genomes and proteomes of tobacco (Nicotiana tabacum L.) and foxglove (Digitalis purpurea L.) plants. MORF9 protein, one of the auxiliary components of the RNA editing complex of organelles (editosome), was found among the most prominent protein partners of adrenodoxin-like [2Fe-2S] tobacco ferredoxins. According to the results obtained from the yeast two-hybrid system, NtMFDX1 and NtMFDX2 of tobacco also bind and interact productively with the previously uncharacterised long non-coding polyadenylated RNA, which, based on its structural features, is capable of regulating the function of a number of components of complexes I (Nad1, Nad5) and III (protein of the cytochrome c synthesis system CcmF) and contributes to the formation of Fe/S-clusters in the corresponding protein complexes of the respiratory chain of plant mitochondria. We found one of the main components of the thiazol synthase complex (mitochondrial protein DpTHI1) to be the partner of ferredoxin DpMFDX2 of Digitalis purpurea. Finally, additional arguments were obtained in favour of the possible participation of MFDX1 and MFDX2 in the very ancient, but only recently described ‘progesterone’ steroid hormonal regulatory system: in leaves of the previously constructed CYP11A1-transgenic tomato plants, only the mature form of mitochondrial cytochrome P450scc (CYP11A1) of mammals is able to enter the mitochondria, where the above-mentioned components of the electron transport chain are localised. In summary, all of the newly revealed interactions of adrenodoxin-like [2Fe-2S] ferredoxins MFDX1 and MFDX2 indicate their participation in a wide range of functions in plant mitochondria.
Keywords	cyp11a1 ; cytochrome p450scc ; mitochondrial ferredoxins mfdx1 and mfdx2 ; transgenic plants ; Science ; Q
Subject code	580
Language	English
Publishing date	2019-12-01T00:00:00Z
Publisher	Sciendo
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A key enzyme of animal steroidogenesis can function in plants enhancing their immunity and accelerating the processes of growth and development.

Shpakovski, George V / Spivak, Svetlana G / Berdichevets, Irina N / Babak, Olga G / Kubrak, Svetlana V / Kilchevsky, Alexander V / Aralov, Andrey V / Slovokhotov, Ivan Yu / Shpakovski, Dmitry G / Baranova, Ekaterina N / Khaliluev, Marat R / Shematorova, Elena K

BMC plant biology

2017 Volume 17, Issue Suppl 1, Page(s) 189

Abstract: Background: The initial stage of the biosynthesis of steroid hormones in animals occurs in the mitochondria of steroidogenic tissues, where cytochrome P450: Results: For a more detailed comparison of steroidogenic systems of Plantae and Animalia, we ... ...

Abstract	Background: The initial stage of the biosynthesis of steroid hormones in animals occurs in the mitochondria of steroidogenic tissues, where cytochrome P450 Results: For a more detailed comparison of steroidogenic systems of Plantae and Animalia, we have created and studied transgenic tobacco and tomato plants efficiently expressing mammalian CYP11A1 cDNA. The detailed phenotypic characterization of plants obtained has shown that through four generations studied, the transgenic tobacco plants have reduced a period of vegetative development (early flowering and maturation of bolls), enlarged biomass and increased productivity (quantity and quality of seeds) as compared to the only empty-vector containing or wild type plants. Moreover, the CYP11A1 transgenic plants show resistance to such fungal pathogen as Botrytis cinerea. Similar valuable phenotypes (the accelerated course of ontogenesis and/or stress resistance) are also visible in two clearly distinct transgenic tomato lines expressing CYP11A1 cDNA: one line (No. 4) has an accelerated rate of vegetative development, while the other (No. 7) has enhanced immunity to abiotic and biotic stresses. The progesterone level in transgenic tobacco and tomato leaves is 3-5 times higher than in the control plants of the wild type. Conclusions: For the first time, we could show the compatibility in vivo of even the most specific components of the systems of biosynthesis of steroid hormones in Plantae and Animalia. The hypothesis is proposed and substantiated that the formation of the above-noted special phenotypes of transgenic plants expressing mammalian CYP11A1 cDNA is due to the increased biosynthesis of progesterone that can be considered as a very ancient bioregulator of plant cells and the first real hormone common to plants and animals.
MeSH term(s)	Agrobacterium/genetics ; Animals ; Botrytis/physiology ; Cattle ; Cholesterol Side-Chain Cleavage Enzyme/genetics ; Cholesterol Side-Chain Cleavage Enzyme/metabolism ; Cloning, Molecular ; Solanum lycopersicum/genetics ; Solanum lycopersicum/immunology ; Solanum lycopersicum/microbiology ; Plants, Genetically Modified ; Progesterone/metabolism ; Steroids/biosynthesis ; Nicotiana/genetics ; Nicotiana/immunology ; Nicotiana/microbiology
Chemical Substances	Steroids ; Progesterone (4G7DS2Q64Y) ; Cholesterol Side-Chain Cleavage Enzyme (EC 1.14.15.6)
Language	English
Publishing date	2017-11-14
Publishing country	England
Document type	Journal Article
ZDB-ID	2059868-3
ISSN	1471-2229 ; 1471-2229
ISSN (online)	1471-2229
ISSN	1471-2229
DOI	10.1186/s12870-017-1123-2
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Article ; Online: Targeted Protection of Mitochondria of Mesophyll Cells in Transgenic CYP11A1 CDNA Expressing Tobacco Plant Leaves After NaCl-Induced Stress Damage

Baranova Ekaterina N. / Khaliluev Marat R. / Spivak Svetlana G. / Bogoutdinova Lilia R. / Klykov Valery N. / Babak Olga G. / Shpakovski Dmitry G. / Kilchevsky Alexander V. / Shematorova Elena K. / Shpakovski George V.

Proceedings of the Latvian Academy of Sciences. Section B, Natural Sciences, Vol 72, Iss 6, Pp 334-

2018 Volume 340

Abstract: Recently we have showed that the expression of the mammalian CYP11A1 cDNA in plants confers their resistance to abiotic and biotic stresses. To determine the role of heterologous expression of cytochrome P450scc cDNA in resistance to ROS (radical oxygen ... ...

Abstract	Recently we have showed that the expression of the mammalian CYP11A1 cDNA in plants confers their resistance to abiotic and biotic stresses. To determine the role of heterologous expression of cytochrome P450scc cDNA in resistance to ROS (radical oxygen species) dependent abiotic stresses, the structural changes of mitochondria and peroxisomes were studied under 150 mM NaCl-induced 14-day salinity treatment on juvenile tobacco plants in in vitro culture. Ultrastructural analysis of mesophyll cells of transgenic tobacco leaves constitutively expressing CYP11A1 cDNA was performed. Under NaCl stress, a change in shape from rounded to elon-gated, reduced section area, formation of branched mitochondria, as well as the emergence of triangular and rhomboid cristae, densification of a mitochondrial matrix, increase in density of contrasting membranes and their thickness were observed in non-transgenic plants. Transgenic plants without stress applied had mitochondria with rounded and elongated shape, twice as small as in non-transgenic plants, with a dense matrix and sinuous cristae. Surprisingly, the effect of NaCl led to increase in size of mitochondria by 1.5 times, decomposition of matrix and the emergence in organelles of light zones presumably containing mitochondrial DNA strands. Thus, the structural organisation of transgenic plant mitochondria under salinity treatment was comparable to that of non-transgenic plants under native conditions. It was also noted that the transgenic plant peroxisomes differed in non-transgenic tobacco both in normal condition and under the action of NaCl. The observed differences in ultrastructural organisation of mitochondria not only support our earlier notion about successful incorporation of the mature P450scc into this organelle, but for the first time demonstrate that the mammalian CYP11A1 signal peptide sequence could be efficiently used in the formation of targeted mitochondria protection of plants from salinity-induced damage.
Keywords	cyp11a1 ; cytochrome p450scc ; salinity ; mitochondrion ultrastructure ; transgenic plants ; Science ; Q
Subject code	580 ; 630
Language	English
Publishing date	2018-12-01T00:00:00Z
Publisher	Sciendo
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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