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Article ; Online: Is it a wrap? Nucleosome interactions of the BRCA1-binding partner, BARD1, steal the scene.

Morris, Joanna R

2021 Volume 28, Issue 9, Page(s) 708–710

MeSH term(s)	Ankyrin Repeat ; BRCA1 Protein/metabolism ; Cryoelectron Microscopy ; DNA Damage ; Fanconi Anemia Complementation Group N Protein/metabolism ; Histone Code ; Histones/metabolism ; Humans ; Mutation, Missense ; Neoplasm Proteins/metabolism ; Neoplasms/enzymology ; Nucleosomes/metabolism ; Phthalazines/pharmacology ; Piperazines/pharmacology ; Protein Binding ; Protein Domains ; Recombinational DNA Repair ; Tumor Suppressor Proteins/chemistry ; Tumor Suppressor Proteins/genetics ; Tumor Suppressor Proteins/metabolism ; Ubiquitin-Protein Ligases/chemistry ; Ubiquitin-Protein Ligases/genetics ; Ubiquitin-Protein Ligases/metabolism ; Ubiquitination
Chemical Substances	BRCA1 Protein ; BRCA1 protein, human ; Fanconi Anemia Complementation Group N Protein ; Histones ; Neoplasm Proteins ; Nucleosomes ; PALB2 protein, human ; Phthalazines ; Piperazines ; Tumor Suppressor Proteins ; BARD1 protein, human (EC 2.3.2.27) ; Ubiquitin-Protein Ligases (EC 2.3.2.27) ; olaparib (WOH1JD9AR8)
Language	English
Publishing date	2021-09-13
Publishing country	United States
Document type	Journal Article
ZDB-ID	2126708-X
ISSN	1545-9985 ; 1545-9993
ISSN (online)	1545-9985
ISSN	1545-9993
DOI	10.1038/s41594-021-00658-7
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Article ; Online: SUMO monoclonal antibodies vary in sensitivity, specificity, and ability to detect types of SUMO conjugate.

Garvin, Alexander J / Lanz, Alexander J / Morris, Joanna R

Scientific reports

2022 Volume 12, Issue 1, Page(s) 21343

Abstract: Monoclonal antibodies (MAb) to members of the Small Ubiquitin-like modifier (SUMO) family are essential tools in the study of cellular SUMOylation. However, many anti-SUMO MAbs are poorly validated, and antibody matching to detection format is without an ...

Abstract	Monoclonal antibodies (MAb) to members of the Small Ubiquitin-like modifier (SUMO) family are essential tools in the study of cellular SUMOylation. However, many anti-SUMO MAbs are poorly validated, and antibody matching to detection format is without an evidence base. Here we test the specificity and sensitivity of twenty-four anti-SUMO MAbs towards monomeric and polymeric SUMO1-4 in dot-blots, immunoblots, immunofluorescence and immunoprecipitation. We find substantial variability between SUMO MAbs for different conjugation states, for detecting increased SUMOylation in response to thirteen different stress agents, and as enrichment reagents for SUMOylated RanGAP1 or KAP1. All four anti-SUMO4 monoclonal antibodies tested cross-reacted wit SUMO2/3, and several SUMO2/3 monoclonal antibodies cross-reacted with SUMO4. These data characterize the specificity of twenty-four anti-SUMO antibodies across commonly used assays, creating an enabling resource for the SUMO research community.
MeSH term(s)	Small Ubiquitin-Related Modifier Proteins/metabolism ; Antibodies, Monoclonal ; Ubiquitin ; Sumoylation ; Immunoprecipitation
Chemical Substances	Small Ubiquitin-Related Modifier Proteins ; Antibodies, Monoclonal ; Ubiquitin
Language	English
Publishing date	2022-12-09
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-25665-6
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Book: Abortion law in transnational perspective

Cook, Rebecca J. / Erdman, Joanna N. / Dickens, Bernard Morris

cases and controversies

(Pennsylvania studies in human rights)

2014

Author's details	edited by Rebecca J. Cook, Joanna N. Erdman, and Bernard M. Dickens
Series title	Pennsylvania studies in human rights
Keywords	Abortion, Induced / legislation & jurisprudence ; Abortion/Law and legislation
Subject code	342.08/4
Language	English
Size	vii, 472 Seiten, 24 cm
Edition	1st ed.
Publisher	University of Pennsylvania Press
Publishing place	Philadelphia
Publishing country	United States
Document type	Book
HBZ-ID	HT018982758
ISBN	978-0-8122-4627-8 ; 0-8122-4627-6
Database	Catalogue ZB MED Medicine, Health

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Shelf mark	Loan status	Location
2016 A 1414	available for loan (reading room)	Lesesaal EG

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Article: Moving Mountains-The BRCA1 Promotion of DNA Resection.

Densham, Ruth M / Morris, Joanna R

Frontiers in molecular biosciences

2019 Volume 6, Page(s) 79

Abstract: DNA double-strand breaks (DSBs) occur in our cells in the context of chromatin. This type of lesion is toxic, entirely preventing genome continuity and causing cell death or terminal arrest. Several repair mechanisms can act on DNA surrounding a DSB, ... ...

Abstract	DNA double-strand breaks (DSBs) occur in our cells in the context of chromatin. This type of lesion is toxic, entirely preventing genome continuity and causing cell death or terminal arrest. Several repair mechanisms can act on DNA surrounding a DSB, only some of which carry a low risk of mutation, so that which repair process is utilized is critical to the stability of genetic material of cells. A key component of repair outcome is the degree of DNA resection directed to either side of the break site. This in turn determines the subsequent forms of repair in which DNA homology plays a part. Here we will focus on chromatin and chromatin-bound complexes which constitute the "mountains" that block resection, with a particular focus on how the breast and ovarian cancer predisposition protein-1 (BRCA1) contributes to repair outcomes through overcoming these blocks.
Language	English
Publishing date	2019-09-03
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2814330-9
ISSN	2296-889X
ISSN	2296-889X
DOI	10.3389/fmolb.2019.00079
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Article ; Online: SUMO monoclonal antibodies vary in sensitivity, specificity, and ability to detect types of SUMO conjugate

Alexander J. Garvin / Alexander J. Lanz / Joanna R. Morris

Scientific Reports, Vol 12, Iss 1, Pp 1-

2022 Volume 16

Abstract: Abstract Monoclonal antibodies (MAb) to members of the Small Ubiquitin-like modifier (SUMO) family are essential tools in the study of cellular SUMOylation. However, many anti-SUMO MAbs are poorly validated, and antibody matching to detection format is ... ...

Abstract	Abstract Monoclonal antibodies (MAb) to members of the Small Ubiquitin-like modifier (SUMO) family are essential tools in the study of cellular SUMOylation. However, many anti-SUMO MAbs are poorly validated, and antibody matching to detection format is without an evidence base. Here we test the specificity and sensitivity of twenty-four anti-SUMO MAbs towards monomeric and polymeric SUMO1-4 in dot-blots, immunoblots, immunofluorescence and immunoprecipitation. We find substantial variability between SUMO MAbs for different conjugation states, for detecting increased SUMOylation in response to thirteen different stress agents, and as enrichment reagents for SUMOylated RanGAP1 or KAP1. All four anti-SUMO4 monoclonal antibodies tested cross-reacted wit SUMO2/3, and several SUMO2/3 monoclonal antibodies cross-reacted with SUMO4. These data characterize the specificity of twenty-four anti-SUMO antibodies across commonly used assays, creating an enabling resource for the SUMO research community.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2022-12-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Exercise-based rehabilitation programmes for pulmonary hypertension.

Morris, Norman R / Kermeen, Fiona D / Jones, Arwel W / Lee, Joanna Yt / Holland, Anne E

The Cochrane database of systematic reviews

2023 Volume 3, Page(s) CD011285

Abstract: Background: Individuals with pulmonary hypertension (PH) have reduced exercise capacity and quality of life. Despite initial concerns that exercise training may worsen symptoms in this group, several studies have reported improvements in functional ... ...

Abstract	Background: Individuals with pulmonary hypertension (PH) have reduced exercise capacity and quality of life. Despite initial concerns that exercise training may worsen symptoms in this group, several studies have reported improvements in functional capacity and well-being following exercise-based rehabilitation. Objectives: To evaluate the benefits and harms of exercise-based rehabilitation for people with PH compared with usual care or no exercise-based rehabilitation. Search methods: We used standard, extensive Cochrane search methods. The latest search date was 28 June 2022. Selection criteria: We included randomised controlled trials (RCTs) in people with PH comparing supervised exercise-based rehabilitation programmes with usual care or no exercise-based rehabilitation. Data collection and analysis: We used standard Cochrane methods. Our primary outcomes were 1. exercise capacity, 2. serious adverse events during the intervention period and 3. health-related quality of life (HRQoL). Our secondary outcomes were 4. cardiopulmonary haemodynamics, 5. Functional Class, 6. clinical worsening during follow-up, 7. mortality and 8. changes in B-type natriuretic peptide. We used GRADE to assess certainty of evidence. Main results: We included eight new studies in the current review, which now includes 14 RCTs. We extracted data from 11 studies. The studies had low- to moderate-certainty evidence with evidence downgraded due to inconsistencies in the data and performance bias. The total number of participants in meta-analyses comparing exercise-based rehabilitation to control groups was 462. The mean age of the participants in the 14 RCTs ranged from 35 to 68 years. Most participants were women and classified as Group I pulmonary arterial hypertension (PAH). Study durations ranged from 3 to 25 weeks. Exercise-based programmes included both inpatient- and outpatient-based rehabilitation that incorporated both upper and lower limb exercise. The mean six-minute walk distance following exercise-based rehabilitation was 48.52 metres higher than control (95% confidence interval (CI) 33.42 to 63.62; I² = 72%; 11 studies, 418 participants; low-certainty evidence), the mean peak oxygen uptake was 2.07 mL/kg/min higher than control (95% CI 1.57 to 2.57; I² = 67%; 7 studies, 314 participants; low-certainty evidence) and the mean peak power was 9.69 W higher than control (95% CI 5.52 to 13.85; I² = 71%; 5 studies, 226 participants; low-certainty evidence). Three studies reported five serious adverse events; however, exercise-based rehabilitation was not associated with an increased risk of serious adverse event (risk difference 0, 95% CI -0.03 to 0.03; I² = 0%; 11 studies, 439 participants; moderate-certainty evidence). The mean change in HRQoL for the 36-item Short Form (SF-36) Physical Component Score was 3.98 points higher (95% CI 1.89 to 6.07; I² = 38%; 5 studies, 187 participants; moderate-certainty evidence) and for the SF-36 Mental Component Score was 3.60 points higher (95% CI 1.21 to 5.98 points; I² = 0%; 5 RCTs, 186 participants; moderate-certainty evidence). There were similar effects in the subgroup analyses for participants with Group 1 PH versus studies of groups with mixed PH. Two studies reported mean reduction in mean pulmonary arterial pressure following exercise-based rehabilitation (mean reduction: 9.29 mmHg, 95% CI -12.96 to -5.61; I² = 0%; 2 studies, 133 participants; low-certainty evidence). Authors' conclusions: In people with PH, supervised exercise-based rehabilitation may result in a large increase in exercise capacity. Changes in exercise capacity remain heterogeneous and cannot be explained by subgroup analysis. It is likely that exercise-based rehabilitation increases HRQoL and is probably not associated with an increased risk of a serious adverse events. Exercise training may result in a large reduction in mean pulmonary arterial pressure. Overall, we assessed the certainty of the evidence to be low for exercise capacity and mean pulmonary arterial pressure, and moderate for HRQoL and adverse events. Future RCTs are needed to inform the application of exercise-based rehabilitation across the spectrum of people with PH, including those with chronic thromboembolic PH, PH with left-sided heart disease and those with more severe disease.
MeSH term(s)	Female ; Humans ; Adult ; Middle Aged ; Aged ; Male ; Hypertension, Pulmonary ; Exercise Therapy/adverse effects ; Quality of Life ; Exercise ; Bias
Language	English
Publishing date	2023-03-22
Publishing country	England
Document type	Systematic Review ; Journal Article ; Review ; Research Support, Non-U.S. Gov't
ISSN	1469-493X
ISSN (online)	1469-493X
DOI	10.1002/14651858.CD011285.pub3
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Article ; Online: A fork in the road: Where homologous recombination and stalled replication fork protection part ways.

Tye, Stephanie / Ronson, George E / Morris, Joanna R

Seminars in cell & developmental biology

2020 Volume 113, Page(s) 14–26

Abstract: In response to replication hindrances, DNA replication forks frequently stall and are remodelled into a four-way junction. In such a structure the annealed nascent strand is thought to resemble a DNA double-strand break and remodelled forks are ... ...

Abstract	In response to replication hindrances, DNA replication forks frequently stall and are remodelled into a four-way junction. In such a structure the annealed nascent strand is thought to resemble a DNA double-strand break and remodelled forks are vulnerable to nuclease attack by MRE11 and DNA2. Proteins that promote the recruitment, loading and stabilisation of RAD51 onto single-stranded DNA for homology search and strand exchange in homologous recombination (HR) repair and inter-strand cross-link repair also act to set up RAD51-mediated protection of nascent DNA at stalled replication forks. However, despite the similarities of these pathways, several lines of evidence indicate that fork protection is not simply analogous to the RAD51 loading step of HR. Protection of stalled forks not only requires separate functions of a number of recombination proteins, but also utilises nucleases important for the resection steps of HR in alternative ways. Here we discuss how fork protection arises and how its differences with HR give insights into the differing contexts of these two pathways.
MeSH term(s)	DNA Damage/genetics ; DNA Replication/genetics ; Genomic Instability/genetics ; Homologous Recombination/genetics ; Humans
Language	English
Publishing date	2020-07-09
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1312473-0
ISSN	1096-3634 ; 1084-9521
ISSN (online)	1096-3634
ISSN	1084-9521
DOI	10.1016/j.semcdb.2020.07.004
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Zs.A 2918: Show issues

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Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article: BRCA1-BARD1: the importance of being in shape.

Daza-Martin, Manuel / Densham, Ruth M / Morris, Joanna R

Molecular & cellular oncology

2019 Volume 6, Issue 6, Page(s) e1656500

Abstract: The breast cancer type-1 susceptibility protein (BRCA1) contributes to genome integrity through homologous recombinational DNA repair and by protecting stalled replication forks from nucleolytic degradation. We recently discovered that fork protection ... ...

Abstract	The breast cancer type-1 susceptibility protein (BRCA1) contributes to genome integrity through homologous recombinational DNA repair and by protecting stalled replication forks from nucleolytic degradation. We recently discovered that fork protection requires a conformational change of BRCA1 unimportant to homologous recombination repair, indicating separate roles for BRCA1 in these pathways.
Language	English
Publishing date	2019-09-11
Publishing country	United States
Document type	Journal Article
ISSN	2372-3556
ISSN	2372-3556
DOI	10.1080/23723556.2019.1656500
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: SUMO, a small, but powerful, regulator of double-strand break repair.

Garvin, Alexander J / Morris, Joanna R

Philosophical transactions of the Royal Society of London. Series B, Biological sciences

2017 Volume 372, Issue 1731

Abstract: The response to a DNA double-stranded break in mammalian cells is a process of sensing and signalling the lesion. It results in halting the cell cycle and local transcription and in the mediation of the DNA repair process itself. The response is launched ...

Abstract	The response to a DNA double-stranded break in mammalian cells is a process of sensing and signalling the lesion. It results in halting the cell cycle and local transcription and in the mediation of the DNA repair process itself. The response is launched through a series of post-translational modification signalling events coordinated by phosphorylation and ubiquitination. More recently modifications of proteins by
MeSH term(s)	Animals ; DNA Breaks, Double-Stranded ; DNA Repair ; Humans ; Mammals/genetics ; Small Ubiquitin-Related Modifier Proteins/metabolism ; Sumoylation
Chemical Substances	Small Ubiquitin-Related Modifier Proteins
Language	English
Publishing date	2017-08-28
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	208382-6
ISSN	1471-2970 ; 0080-4622 ; 0264-3839 ; 0962-8436
ISSN (online)	1471-2970
ISSN	0080-4622 ; 0264-3839 ; 0962-8436
DOI	10.1098/rstb.2016.0281
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Article ; Online: SUMO in the DNA Double-Stranded Break Response: Similarities, Differences, and Cooperation with Ubiquitin.

Morris, Joanna R / Garvin, Alexander J

Journal of molecular biology

2017 Volume 429, Issue 22, Page(s) 3376–3387

Abstract: In recent years, our knowledge of the varied role that ubiquitination plays in promoting signal amplification, novel protein interactions, and protein turnover has progressed rapidly. This is particularly remarkable in the examination of how DNA double- ... ...

Abstract	In recent years, our knowledge of the varied role that ubiquitination plays in promoting signal amplification, novel protein interactions, and protein turnover has progressed rapidly. This is particularly remarkable in the examination of how DNA double-stranded breaks (DSBs) are repaired, with many components of the ubiquitin (Ub) conjugation, de-conjugation, and recognition machinery now identified as key factors in DSB repair. In addition, a member of the Ub-like family, small Ub-like modifier (SUMO), has also been recognised as integral for efficient repair. Here, we summarise our emerging understanding of SUMOylation both as a distinct modification and as a cooperative modification with Ub, using the cellular response to DNA DSBs as the primary setting to compare these modifications.
MeSH term(s)	DNA Breaks, Double-Stranded ; DNA Repair ; Eukaryotic Cells/enzymology ; Eukaryotic Cells/physiology ; Small Ubiquitin-Related Modifier Proteins/metabolism ; Sumoylation ; Ubiquitin/metabolism
Chemical Substances	Small Ubiquitin-Related Modifier Proteins ; Ubiquitin
Language	English
Publishing date	2017-05-17
Publishing country	Netherlands
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	80229-3
ISSN	1089-8638 ; 0022-2836
ISSN (online)	1089-8638
ISSN	0022-2836
DOI	10.1016/j.jmb.2017.05.012
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Zs.A 867: Show issues

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