LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 722

Search options

  1. Book ; Online ; Conference proceedings: Tumor Necrosis Factor: Molecular and Cellular Biology and Clinical Relevance

    Fiers, W.

    4th International Conference on Tumor Necrosis Factor and Related Cytokines, Veldhoven, May 1992

    1993  

    Keywords Immunology ; Oncology ; Biochemistry ; Infectious Diseases ; Molecular Biology ; Pathology ; Pharmacology ; Toxicology
    Language English
    Size 1 Online-Ressource (X + 256 S.)
    Publisher S. Karger
    Publishing place Basel
    Document type Book ; Online ; Conference proceedings
    HBZ-ID HT019541933
    ISBN 978-3-318-05220-6 ; 3-318-05220-5
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article: Cloning and expression of human interferon-beta: from bc to ac.

    Fiers, W

    Verhandelingen - Koninklijke Academie voor Geneeskunde van Belgie

    2009  Volume 71, Issue 1-2, Page(s) 43–50

    Abstract: In 1963 I started the Laboratory of Molecular Biology (LMB) at the University of Ghent. Molecular Biology was then a new scientific discipline. Nucleic acids (NA) could be sequenced, manipulated, and recombined to form genetic information which never ... ...

    Abstract In 1963 I started the Laboratory of Molecular Biology (LMB) at the University of Ghent. Molecular Biology was then a new scientific discipline. Nucleic acids (NA) could be sequenced, manipulated, and recombined to form genetic information which never before had existed. Cloning of DNA-segments allowed multiplying a single molecule a billion-fold. By 1975 recombinant DNA-technology had sufficiently progressed that one could start the pursuit of a medically important goal. Our choice was to go for Interferon, a mysterious substance which could protect against viral infection, and might possibly be used as an anti-cancer agent if available in unlimited quantities. Fortunately, Piet De Somer, then Rector of the KUL, was one of the pioneers in interferon research. He encouraged his young colleague Erik De Clercq to collaborate with us. Erik brought extensive interferon expertise and reagents to the collaboration. But molecular biologists work with NA and interferon is a protein. There was a missing link which was provided by Jean Content of the Brussels Pasteur Institute. Jean had developed a system to convert interferon mRNA into protein, which was send to Leuven for quantification. This close collaboration between 3 laboratories led in January 1980 to the cloning of the human fibroblast, now interferon-beta, gene, and to the primary structure of the protein. However, 2 months earlier, Tadatsugu Taniguchi had succeeded already to obtain such a clone. But we were the first to express the clone in E. coli, and this was the definite proof that the cloned gene coded for human interferon-beta.
    MeSH term(s) Cloning, Molecular ; DNA, Recombinant ; Gene Expression ; Humans ; Interferon-beta/genetics ; Molecular Biology
    Chemical Substances DNA, Recombinant ; Interferon-beta (77238-31-4)
    Language English
    Publishing date 2009
    Publishing country Belgium
    Document type Journal Article ; Review
    ZDB-ID 196359-4
    ISSN 0302-6469 ; 0372-610X
    ISSN 0302-6469 ; 0372-610X
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 802: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Cytokine research in Belgium.

    Fiers, Walter

    Cytokine & growth factor reviews

    2011  Volume 22, Issue 5-6, Page(s) 255–256

    MeSH term(s) Animals ; Belgium ; Biomedical Research ; Cytokines ; Humans
    Chemical Substances Cytokines
    Language English
    Publishing date 2011-10
    Publishing country England
    Document type Editorial
    ZDB-ID 1330534-7
    ISSN 1879-0305 ; 1359-6101
    ISSN (online) 1879-0305
    ISSN 1359-6101
    DOI 10.1016/j.cytogfr.2011.11.008
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2653: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: A universal vaccine against influenza.

    Fiers, W

    Bulletin et memoires de l'Academie royale de medecine de Belgique

    2006  Volume 161, Issue 5, Page(s) 237–239

    MeSH term(s) Humans ; Influenza Vaccines ; Influenza, Human/prevention & control
    Chemical Substances Influenza Vaccines
    Language English
    Publishing date 2006
    Publishing country Belgium
    Document type Journal Article
    ZDB-ID 1445946-2
    ISSN 0377-8231
    ISSN 0377-8231
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ua VI Zs.7: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article: The determination of ribonuclease activity.

    FIERS, W

    Analytical biochemistry

    2003  Volume 2, Page(s) 126–139

    MeSH term(s) Ribonucleases/chemistry
    Chemical Substances Ribonucleases (EC 3.1.-)
    Language English
    Publishing date 2003-04-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1110-1
    ISSN 1096-0309 ; 0003-2697
    ISSN (online) 1096-0309
    ISSN 0003-2697
    DOI 10.1016/0003-2697(61)90061-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 389: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: ABA signalling promotes cell totipotency in the shoot apex of germinating embryos.

    Chen, Baojian / Fiers, Martijn / Dekkers, Bas J W / Maas, Lena / van Esse, G Wilma / Angenent, Gerco C / Zhao, Yang / Boutilier, Kim

    Journal of experimental botany

    2021  Volume 72, Issue 18, Page(s) 6418–6436

    Abstract: Somatic embryogenesis (SE) is a type of induced cell totipotency where embryos develop from vegetative tissues of the plant instead of from gamete fusion after fertilization. SE can be induced in vitro by exposing explants to growth regulators, such as ... ...

    Abstract Somatic embryogenesis (SE) is a type of induced cell totipotency where embryos develop from vegetative tissues of the plant instead of from gamete fusion after fertilization. SE can be induced in vitro by exposing explants to growth regulators, such as the auxinic herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The plant hormone abscisic acid (ABA) has been proposed to be a downstream signalling component at the intersection between 2,4-D- and stress-induced SE, but it is not known how these pathways interact to induce cell totipotency. Here we show that 2,4-D-induced SE from the shoot apex of germinating Arabidopsis thaliana seeds is characterized by transcriptional maintenance of an ABA-dependent seed maturation pathway. Molecular-genetic analysis of Arabidopsis mutants revealed a role for ABA in promoting SE at three different levels: ABA biosynthesis, ABA receptor complex signalling, and ABA-mediated transcription, with essential roles for the ABSCISIC ACID INSENSITIVE 3 (ABI3) and ABI4 transcription factors. Our data suggest that the ability of mature Arabidopsis embryos to maintain the ABA seed maturation environment is an important first step in establishing competence for auxin-induced cell totipotency. This finding provides further support for the role of ABA in directing processes other than abiotic stress response.
    MeSH term(s) Abscisic Acid ; Arabidopsis/genetics ; Arabidopsis/metabolism ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Gene Expression Regulation, Plant ; Germination ; Plant Growth Regulators ; Seeds/metabolism
    Chemical Substances Arabidopsis Proteins ; Plant Growth Regulators ; Abscisic Acid (72S9A8J5GW)
    Language English
    Publishing date 2021-06-26
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2976-2
    ISSN 1460-2431 ; 0022-0957
    ISSN (online) 1460-2431
    ISSN 0022-0957
    DOI 10.1093/jxb/erab306
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 55/29: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article ; Online: M2-based influenza vaccines: recent advances and clinical potential.

    Kolpe, Annasaheb / Schepens, Bert / Fiers, Walter / Saelens, Xavier

    Expert review of vaccines

    2017  Volume 16, Issue 2, Page(s) 123–136

    Abstract: Introduction: Current influenza vaccines can prevent disease caused by influenza viruses but require annual administration and almost yearly reformulation. An attractive alternative approach would be to use a vaccine that provides broad and, ideally, ... ...

    Abstract Introduction: Current influenza vaccines can prevent disease caused by influenza viruses but require annual administration and almost yearly reformulation. An attractive alternative approach would be to use a vaccine that provides broad and, ideally, lifelong protection against all influenza A and B virus strains. The extracellular domain of matrix protein 2 (M2e) of influenza A viruses is conserved and thus fits well in such a broadly protective vaccine. Areas covered: Recent advances in M2e vaccine design, the mode of action of M2e-based immunity and clinical progress of M2-based influenza vaccines. Expert commentary: Many M2e vaccine have been successfully tested for efficacy against a panel of divergent influenza viruses in animal models. More recently, clinical studies have been conducted with M2e vaccine candidates, which demonstrated their safety and immunogenicity in humans. Efficacy studies in humans are still needed to provide evidence that an M2e-based vaccine can protect against human influenza.
    MeSH term(s) Animals ; Clinical Trials as Topic ; Disease Models, Animal ; Drug Discovery/trends ; Drug Evaluation, Preclinical ; Humans ; Influenza Vaccines/adverse effects ; Influenza Vaccines/genetics ; Influenza Vaccines/immunology ; Vaccines, Synthetic/adverse effects ; Vaccines, Synthetic/genetics ; Vaccines, Synthetic/immunology ; Viral Matrix Proteins/genetics ; Viral Matrix Proteins/immunology
    Chemical Substances Influenza Vaccines ; M2 protein, Influenza A virus ; Vaccines, Synthetic ; Viral Matrix Proteins
    Language English
    Publishing date 2017-02
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2181284-6
    ISSN 1744-8395 ; 1476-0584
    ISSN (online) 1744-8395
    ISSN 1476-0584
    DOI 10.1080/14760584.2017.1240041
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6077: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article: [Cancer: what does the future bring?].

    Fiers, W

    Verhandelingen - Koninklijke Academie voor Geneeskunde van Belgie

    2001  Volume 63, Issue 2, Page(s) 153–7; discussion 158–60

    MeSH term(s) Forecasting ; Humans ; Molecular Biology ; Neoplasms/genetics ; Neoplasms/therapy
    Language English
    Publishing date 2001
    Publishing country Belgium
    Document type Journal Article ; Review
    ZDB-ID 196359-4
    ISSN 0302-6469 ; 0372-610X
    ISSN 0302-6469 ; 0372-610X
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 802: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article ; Online: ABA signalling promotes cell totipotency in the shoot apex of germinating embryos

    Chen, B. / Fiers, M.A. / Dekkers, S.J.W. / Maas, L. / van Esse, G.W. / Angenent, G.C. / Zhao, Yang / Boutilier, K.A.

    Journal of Experimental Botany

    2021  Volume 72, Issue 18

    Abstract: Somatic embryogenesis (SE) is a type of induced cell totipotency where embryos develop from vegetative tissues of the plant instead of from gamete fusion after fertilization. SE can be induced in vitro by exposing explants to growth regulators, such as ... ...

    Abstract Somatic embryogenesis (SE) is a type of induced cell totipotency where embryos develop from vegetative tissues of the plant instead of from gamete fusion after fertilization. SE can be induced in vitro by exposing explants to growth regulators, such as the auxinic herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The plant hormone abscisic acid (ABA) has been proposed to be a downstream signalling component at the intersection between 2,4-D- and stress-induced SE, but it is not known how these pathways interact to induce cell totipotency. Here we show that 2,4-D-induced SE from the shoot apex of germinating Arabidopsis thaliana seeds is characterized by transcriptional maintenance of an ABA-dependent seed maturation pathway. Molecular–genetic analysis of Arabidopsis mutants revealed a role for ABA in promoting SE at three different levels: ABA biosynthesis, ABA receptor complex signalling, and ABA-mediated transcription, with essential roles for the ABSCISIC ACID INSENSITIVE 3 (ABI3) and ABI4 transcription factors. Our data suggest that the ability of mature Arabidopsis embryos to maintain the ABA seed maturation environment is an important first step in establishing competence for auxin-induced cell totipotency. This finding provides further support for the role of ABA in directing processes other than abiotic stress response.
    Keywords 2,4-D ; ABA ; Arabidopsis ; mature zygotic embryo ; seed maturation ; somatic embryogenesis ; totipotency
    Subject code 580
    Language English
    Publishing country nl
    Document type Article ; Online
    ZDB-ID 2976-2
    ISSN 1460-2431 ; 0022-0957
    ISSN (online) 1460-2431
    ISSN 0022-0957
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 55/29: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  10. Article: Mapping gene regulatory networks from single-cell omics data

    Fiers, Mark W E J / Minnoye, Liesbeth / Aibar, Sara / Bravo González-Blas, Carmen / Kalender Atak, Zeynep / Aerts, Stein

    Briefings in functional genomics. 2018 Jan. 12, v. 17, no. 4

    2018  

    Abstract: Single-cell techniques are advancing rapidly and are yielding unprecedented insight into cellular heterogeneity. Mapping the gene regulatory networks (GRNs) underlying cell states provides attractive opportunities to mechanistically understand this ... ...

    Abstract Single-cell techniques are advancing rapidly and are yielding unprecedented insight into cellular heterogeneity. Mapping the gene regulatory networks (GRNs) underlying cell states provides attractive opportunities to mechanistically understand this heterogeneity. In this review, we discuss recently emerging methods to map GRNs from single-cell transcriptomics data, tackling the challenge of increased noise levels and data sparsity compared with bulk data, alongside increasing data volumes. Next, we discuss how new techniques for single-cell epigenomics, such as single-cell ATAC-seq and single-cell DNA methylation profiling, can be used to decipher gene regulatory programmes. We finally look forward to the application of single-cell multi-omics and perturbation techniques that will likely play important roles for GRN inference in the future.
    Keywords DNA methylation ; epigenetics ; gene regulatory networks ; transcriptomics
    Language English
    Dates of publication 2018-0112
    Size p. 246-254.
    Publishing place Oxford University Press
    Document type Article
    ZDB-ID 2540916-5
    ISSN 2041-2657 ; 2041-2649 ; 2041-2647
    ISSN (online) 2041-2657
    ISSN 2041-2649 ; 2041-2647
    DOI 10.1093/bfgp/elx046
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6076: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top