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  1. Article ; Online: The Golgi checkpoint: Golgi unlinking during G2 is necessary for spindle formation and cytokinesis.

    Mascanzoni, Fabiola / Ayala, Inmaculada / Iannitti, Roberta / Luini, Alberto / Colanzi, Antonino

    Life science alliance

    2024  Volume 7, Issue 5

    Abstract: Entry into mitosis requires not only correct DNA replication but also extensive cell reorganization, including the separation of the Golgi ribbon into isolated stacks. To understand the significance of pre-mitotic Golgi reorganization, we devised a ... ...

    Abstract Entry into mitosis requires not only correct DNA replication but also extensive cell reorganization, including the separation of the Golgi ribbon into isolated stacks. To understand the significance of pre-mitotic Golgi reorganization, we devised a strategy to first block Golgi segregation, with the consequent G2-arrest, and then force entry into mitosis. We found that the cells forced to enter mitosis with an intact Golgi ribbon showed remarkable cell division defects, including spindle multipolarity and binucleation. The spindle defects were caused by reduced levels at the centrosome of the kinase Aurora-A, a pivotal spindle formation regulator controlled by Golgi segregation. Overexpression of Aurora-A rescued spindle formation, indicating a crucial role of the Golgi-dependent recruitment of Aurora-A at the centrosome. Thus, our results reveal that alterations of the pre-mitotic Golgi segregation in G2 have profound consequences on the fidelity of later mitotic processes and represent potential risk factors for cell transformation and cancer development.
    MeSH term(s) Cytokinesis ; Mitosis ; Golgi Apparatus ; Centrosome
    Language English
    Publishing date 2024-03-13
    Publishing country United States
    Document type Journal Article
    ISSN 2575-1077
    ISSN (online) 2575-1077
    DOI 10.26508/lsa.202302469
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Endomembrane-Based Signaling by GPCRs and G-Proteins.

    Liccardo, Federica / Luini, Alberto / Di Martino, Rosaria

    Cells

    2022  Volume 11, Issue 3

    Abstract: G-protein-coupled receptors (GPCRs) and G-proteins have a range of roles in many physiological and pathological processes and are among the most studied signaling proteins. A plethora of extracellular stimuli can activate the GPCR and can elicit distinct ...

    Abstract G-protein-coupled receptors (GPCRs) and G-proteins have a range of roles in many physiological and pathological processes and are among the most studied signaling proteins. A plethora of extracellular stimuli can activate the GPCR and can elicit distinct intracellular responses through the activation of specific transduction pathways. For many years, biologists thought that GPCR signaling occurred entirely on the plasma membrane. However, in recent decades, many lines of evidence have proved that the GPCRs and G-proteins may reside on endomembranes and can start or propagate signaling pathways through the organelles that form the secretory route. How these alternative intracellular signaling pathways of the GPCR and G-proteins influence the physiological and pathological function of the endomembranes is still under investigation. Here, we review the general role and classification of GPCRs and G-proteins with a focus on their signaling pathways in the membrane transport apparatus.
    MeSH term(s) Cell Membrane/metabolism ; GTP-Binding Proteins/metabolism ; Receptors, G-Protein-Coupled/metabolism ; Signal Transduction/physiology
    Chemical Substances Receptors, G-Protein-Coupled ; GTP-Binding Proteins (EC 3.6.1.-)
    Language English
    Publishing date 2022-02-03
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells11030528
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The Diffusion Model of Intra-Golgi Transport Has Limited Power.

    Beznoussenko, Galina V / Bejan, Andrei Iu / Parashuraman, Seetharaman / Luini, Alberto / Kweon, Hee-Seok / Mironov, Alexander A

    International journal of molecular sciences

    2023  Volume 24, Issue 2

    Abstract: The Golgi complex (GC) is the main station along the cell biosecretory pathway. Until now, mechanisms of intra-Golgi transport (IGT) have remained unclear. Herein, we confirm that the goodness-of-fit of the regression lines describing the exit of a cargo ...

    Abstract The Golgi complex (GC) is the main station along the cell biosecretory pathway. Until now, mechanisms of intra-Golgi transport (IGT) have remained unclear. Herein, we confirm that the goodness-of-fit of the regression lines describing the exit of a cargo from the Golgi zone (GZ) corresponds to an exponential decay. When the GC was empty before the re-initiation of the intra-Golgi transport, this parameter of the curves describing the kinetics of different cargoes (which are deleted in Golgi vesicles) with different diffusional mobilities within the GZ as well as their exit from the GZ was maximal for the piecewise nonlinear regression, wherein the first segment was horizontal, while the second segment was similar to the exponential decay. The kinetic curve describing cargo exit from the GC per se resembled a linear decay. The Monte-Carlo simulation revealed that such curves reflect the role of microtubule growth in cells with a central GC or the random hovering of ministacks in cells lacking a microtubule. The synchronization of cargo exit from the GC already filled with a cargo using the wave synchronization protocol did not reveal the equilibration of cargo within a Golgi stack, which would be expected from the diffusion model (DM) of IGT. Moreover, not all cisternae are connected to each other in mini-stacks that are transporting membrane proteins. Finally, the kinetics of post-Golgi carriers and the important role of SNAREs for IGT at different level of IGT also argue against the DM of IGT.
    MeSH term(s) Biological Transport ; Diffusion ; Golgi Apparatus/metabolism ; Protein Transport
    Language English
    Publishing date 2023-01-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24021375
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  4. Article ; Online: The efficacy in shoulder range of motion of a snapping manual maneuver added to a standardized exercise protocol in axillary web syndrome: a randomized controlled trial.

    Sandrin, Fabio / Nevola Teixeira, Luiz Felipe / Garavaglia, Manfredi / Gandini, Sara / Simoncini, Maria Claudia / Luini, Alberto

    Acta oncologica (Stockholm, Sweden)

    2023  Volume 62, Issue 8, Page(s) 969–976

    Abstract: Purpose: Axillary Web Syndrome (AWS) is a common sequela after surgical axillary lymph node dissection (ALND) often manifesting with reduced range of motion (ROM) of the limb, which requires rehabilitation. Notwithstanding, a standardized rehabilitation ...

    Abstract Purpose: Axillary Web Syndrome (AWS) is a common sequela after surgical axillary lymph node dissection (ALND) often manifesting with reduced range of motion (ROM) of the limb, which requires rehabilitation. Notwithstanding, a standardized rehabilitation protocol is currently lacking in clinical practice. Our primary objective was therefore to evaluate the effectiveness of the use of a snapping manual maneuver (SMM, used in our clinical practice) to increase ROM during abduction (ABD) when compared with a standardized stretching exercise (SSE) protocol. A three-year follow-up of the enrolled patients was also carried out to determine the incidence of Breast Cancer-Related Lymphedema (BCRL).
    Materials and methods: Between July 2013 and January 2019, we conducted a single-blinded randomized clinical trial. A total of 60 patients, who underwent ALND in our hospital, came to our clinic under medical advice or on voluntary access and reported AWS symptoms. The patients were randomly assigned into two equally divided groups. The treatment of group one consists in the execution of a supervised SSEs protocol, while group two additionally received a manual snapping maneuver. Patients of both groups received two treatment sessions within two weeks. At the end of the session, they were asked to continue the exercises autonomously on a daily basis, three times per day, for one month.
    Results: There were no statically significant differences in ROM at our one-month follow-up and the incidence of BCRL was equally distributed after three years.
    Conclusions: The use of the manual snapping maneuver in addition to stretching once per week for two weeks does not appear to improve the outcome of the patients in comparison with stretching alone and does not appear to be related to lymphedema in our 3 years follow-up.
    MeSH term(s) Humans ; Disease Progression ; Exercise ; Extremities ; Lymphedema/etiology ; Lymphedema/therapy ; Range of Motion, Articular ; Shoulder ; Axilla
    Language English
    Publishing date 2023-09-26
    Publishing country Sweden
    Document type Journal Article ; Randomized Controlled Trial
    ZDB-ID 896449-x
    ISSN 1651-226X ; 0349-652X ; 0284-186X ; 1100-1704
    ISSN (online) 1651-226X
    ISSN 0349-652X ; 0284-186X ; 1100-1704
    DOI 10.1080/0284186X.2023.2241995
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Endomembrane-Based Signaling by GPCRs and G-Proteins

    Federica Liccardo / Alberto Luini / Rosaria Di Martino

    Cells, Vol 11, Iss 528, p

    2022  Volume 528

    Abstract: G-protein-coupled receptors (GPCRs) and G-proteins have a range of roles in many physiological and pathological processes and are among the most studied signaling proteins. A plethora of extracellular stimuli can activate the GPCR and can elicit distinct ...

    Abstract G-protein-coupled receptors (GPCRs) and G-proteins have a range of roles in many physiological and pathological processes and are among the most studied signaling proteins. A plethora of extracellular stimuli can activate the GPCR and can elicit distinct intracellular responses through the activation of specific transduction pathways. For many years, biologists thought that GPCR signaling occurred entirely on the plasma membrane. However, in recent decades, many lines of evidence have proved that the GPCRs and G-proteins may reside on endomembranes and can start or propagate signaling pathways through the organelles that form the secretory route. How these alternative intracellular signaling pathways of the GPCR and G-proteins influence the physiological and pathological function of the endomembranes is still under investigation. Here, we review the general role and classification of GPCRs and G-proteins with a focus on their signaling pathways in the membrane transport apparatus.
    Keywords secretory pathway ; endomembrane signaling ; GPCR ; G-protein ; Golgi apparatus ; endosomes ; Biology (General) ; QH301-705.5
    Subject code 572
    Language English
    Publishing date 2022-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Calcium flow at ER-TGN contact sites facilitates secretory cargo export.

    Ramazanov, Bulat R / Parchure, Anup / Di Martino, Rosaria / Kumar, Abhishek / Chung, Minhwan / Kim, Yeongho / Griesbeck, Oliver / Schwartz, Martin A / Luini, Alberto / von Blume, Julia

    Molecular biology of the cell

    2024  Volume 35, Issue 4, Page(s) ar50

    Abstract: ... ...

    Abstract Ca
    MeSH term(s) Calcium/metabolism ; trans-Golgi Network/metabolism ; Biological Transport ; Protein Transport ; Endoplasmic Reticulum/metabolism ; Proteins/metabolism ; Carrier Proteins/metabolism
    Chemical Substances Calcium (SY7Q814VUP) ; Proteins ; Carrier Proteins
    Language English
    Publishing date 2024-01-31
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1098979-1
    ISSN 1939-4586 ; 1059-1524
    ISSN (online) 1939-4586
    ISSN 1059-1524
    DOI 10.1091/mbc.E23-03-0099
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2853: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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    Zs.MO 410: Show issues

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  7. Article: A brief history of the cisternal progression-maturation model.

    Luini, Alberto

    Cellular logistics

    2011  Volume 1, Issue 1, Page(s) 6–11

    Language English
    Publishing date 2011-05-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2682440-1
    ISSN 2159-2799 ; 2159-2780
    ISSN (online) 2159-2799
    ISSN 2159-2780
    DOI 10.4161/cl.1.1.14693
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  8. Article ; Online: The Golgi complex: 120 years and it doesn't show.

    De Matteis, Maria Antonietta / Corda, Daniela / Luini, Alberto

    FEBS letters

    2019  Volume 593, Issue 17, Page(s) 2277–2279

    MeSH term(s) Biological Transport ; Golgi Apparatus/metabolism ; Homeostasis ; Lipids/biosynthesis ; Signal Transduction
    Chemical Substances Lipids
    Language English
    Publishing date 2019-09-07
    Publishing country England
    Document type Editorial ; Introductory Journal Article
    ZDB-ID 212746-5
    ISSN 1873-3468 ; 0014-5793
    ISSN (online) 1873-3468
    ISSN 0014-5793
    DOI 10.1002/1873-3468.13577
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    In stock of ZB MED Cologne/Königswinter

    Zs.B 282: Show issues Location:
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  9. Article: Cancer metabolic features allow discrimination of tumor from white blood cells by label-free multimodal optical imaging.

    Mangini, Maria / Ferrara, Maria Antonietta / Zito, Gianluigi / Managò, Stefano / Luini, Alberto / De Luca, Anna Chiara / Coppola, Giuseppe

    Frontiers in bioengineering and biotechnology

    2023  Volume 11, Page(s) 1057216

    Abstract: Circulating tumor cells (CTCs) are tumor cells that have penetrated the circulatory system preserving tumor properties and heterogeneity. Detection and characterization of CTCs has high potential clinical values and many technologies have been developed ... ...

    Abstract Circulating tumor cells (CTCs) are tumor cells that have penetrated the circulatory system preserving tumor properties and heterogeneity. Detection and characterization of CTCs has high potential clinical values and many technologies have been developed for CTC identification. These approaches remain challenged by the extraordinary rarity of CTCs and the difficulty of efficiently distinguishing cancer from the much larger number of white blood cells in the bloodstream. Consequently, there is still a need for efficient and rapid methods to capture the broad spectrum of tumor cells circulating in the blood. Herein, we exploit the peculiarities of cancer metabolism for discriminating cancer from WBCs. Using deuterated glucose and Raman microscopy we show that a) the known ability of cancer cells to take up glucose at greatly increased rates compared to non-cancer cells results in the lipid generation and accumulation into lipid droplets and, b) by contrast, leukocytes do not appear to generate visible LDs. The difference in LD abundance is such that it provides a reliable parameter for distinguishing cancer from blood cells. For LD sensitive detections in a cell at rates suitable for screening purposes, we test a polarization-sensitive digital holographic imaging (PSDHI) technique that detects the birefringent properties of the LDs. By using polarization-sensitive digital holographic imaging, cancer cells (prostate cancer, PC3 and hepatocarcinoma cells, HepG2) can be rapidly discriminated from leukocytes with reliability close to 100%. The combined Raman and PSDHI microscopy platform lays the foundations for the future development of a new label-free, simple and universally applicable cancer cells' isolation method.
    Language English
    Publishing date 2023-02-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2023.1057216
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  10. Article ; Online: Regulation of cargo export and sorting at the trans-Golgi network.

    Di Martino, Rosaria / Sticco, Lucia / Luini, Alberto

    FEBS letters

    2019  Volume 593, Issue 17, Page(s) 2306–2318

    Abstract: The sorting and distribution to different final destinations of roughly a third of the membrane and secreted proteins occurs at the level of the trans-Golgi network (TGN). This TGN mission involves efficient mechanisms of cargo recognition and activation ...

    Abstract The sorting and distribution to different final destinations of roughly a third of the membrane and secreted proteins occurs at the level of the trans-Golgi network (TGN). This TGN mission involves efficient mechanisms of cargo recognition and activation of specific signalling pathways. This is important because protein localization is strictly connected with function, and many aberrant phenotypes may occur when a protein is missorted to the wrong cellular compartment. In this review, we briefly summarize the principal players known to be involved in TGN functions, highlighting the importance of regulatory signalling pathways and also the pathological outcomes of aberrant sorting and export events from the TGN compartment.
    MeSH term(s) Animals ; Humans ; Protein Transport ; Signal Transduction ; trans-Golgi Network/metabolism
    Language English
    Publishing date 2019-08-22
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 212746-5
    ISSN 1873-3468 ; 0014-5793
    ISSN (online) 1873-3468
    ISSN 0014-5793
    DOI 10.1002/1873-3468.13572
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