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  1. Article ; Online: Morphology and Composition of Immunodiffusion Precipitin Complexes Evaluated via Microscopy and Proteomics.

    Jayawardena, Imanda / Wilson, Kirsty / Plebanski, Magdalena / Grøndahl, Lisbeth / Corrie, Simon

    Journal of proteome research

    2021  Volume 20, Issue 5, Page(s) 2618–2627

    Abstract: New approaches to rapid, simple, ...

    Abstract New approaches to rapid, simple,
    MeSH term(s) COVID-19 ; Communicable Disease Control ; Humans ; Immunodiffusion ; Microscopy ; Pandemics ; Precipitins ; Proteomics ; SARS-CoV-2
    Chemical Substances Precipitins
    Language English
    Publishing date 2021-04-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2078618-9
    ISSN 1535-3907 ; 1535-3893
    ISSN (online) 1535-3907
    ISSN 1535-3893
    DOI 10.1021/acs.jproteome.0c01042
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6189: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: Morphology and Composition of Immunodiffusion Precipitin Complexes Evaluated via Microscopy and Proteomics

    Jayawardena, Imanda / Wilson, Kirsty / Plebanski, Magdalena / Grøndahl, Lisbeth / Corrie, Simon

    Journal of proteome research. 2021 Apr. 06, v. 20, no. 5

    2021  

    Abstract: New approaches to rapid, simple, in vitro diagnostic immunoassays that do not rely on centralized laboratory facilities are urgently needed for disease diagnosis and to inform treatment strategies. The recent and ongoing COVID-19 pandemic has emphasized ... ...

    Abstract New approaches to rapid, simple, in vitro diagnostic immunoassays that do not rely on centralized laboratory facilities are urgently needed for disease diagnosis and to inform treatment strategies. The recent and ongoing COVID-19 pandemic has emphasized that rapid diagnostics are needed to help guide government policies on quarantines, social distancing measures, and community lockdowns. A common approach to developing new immunoassays is to modify existing platforms (e.g., automated ELISA and lateral flow assays) for the new analyte, even though this does not address the drawbacks of existing platforms. An alternate approach is to search for robust assays that have been superseded but could in fact solve important challenges using modern technologies. Immunodiffusion is one such platform based on unique “precipitin ring” patterns formed in gels or paper following interactions between proteins and cognate antibodies in diffusion/reaction systems. Herein, we investigate the microstructure of these precipitin rings using a combination of fluorescence and electron microscopy and also perform a mass spectrometry investigation to determine the proteomic composition of the rings. We observed that the rings were composed of microparticles, which we termed “precipitin complexes”, and that these complexes were composed of at least 19 key proteins, including immunoglobulins and complement factors along with a range of plasma proteins, possibly related to immune complexes and/or high-density lipoprotein particles. This information will be useful in developing new in vitro diagnostics using reaction/diffusion systems—techniques that require a single assay step and that only require calibrated length measurements for target protein quantification.
    Keywords COVID-19 infection ; complement ; diagnostic techniques ; disease diagnosis ; electron microscopy ; fluorescence ; high density lipoprotein ; immunoglobulins ; mass spectrometry ; microstructure ; paper ; proteome ; proteomics ; research
    Language English
    Dates of publication 2021-0406
    Size p. 2618-2627.
    Publishing place American Chemical Society
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 2078618-9
    ISSN 1535-3907 ; 1535-3893
    ISSN (online) 1535-3907
    ISSN 1535-3893
    DOI 10.1021/acs.jproteome.0c01042
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6189: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Migration of BTEX and phthalates from natural rubber latex balloons obtained from the Sri Lankan market.

    Jayawardena, Imanda / Godakumbura, Pahan I / Prashantha, M A B

    SpringerPlus

    2016  Volume 5, Page(s) 20

    Abstract: The current study evaluates the migration of benzene, toluene, ethylbenzene, xylene (BTEX) and phthalates into artificial saliva from natural rubber latex (NRL) balloons available for sale in Sri Lanka. It was discovered that at least one BTEX compound ... ...

    Abstract The current study evaluates the migration of benzene, toluene, ethylbenzene, xylene (BTEX) and phthalates into artificial saliva from natural rubber latex (NRL) balloons available for sale in Sri Lanka. It was discovered that at least one BTEX compound migrated from almost all the brands. The migration of four phthalates; diethyl phthalate, dibutyl phthalate, di-isobutyl phthalate and butyl benzyl phthalate were also observed. Migratory levels of BTEX and phthalates in most of the balloon brands were above the permissible levels set by the European Union. Assessment of factors affecting the migratory levels indicated migration under active mouthing conditions and migration from the neck region of the balloons were significantly higher. The migratory levels were observed to decrease with storage time, and in certain brands the BTEX levels decreased below the permissible level. One-way ANOVA indicated no significant differences (p ≥ 0.05) in migratory levels of each individual compound within the same brand for both BTEX and phthalates. When compared among different brands, BTEX levels indicated significant differences (p ≤ 0.05), while phthalate levels were observed to not be significantly different (p ≥ 0.05). A significant difference was also observed (p ≤ 0.05) among the migratory levels of compounds under each test condition evaluated as factors affecting the migratory level. Furthermore, the solvent based colorants added to color the latex were found to be the source of BTEX and phthalates in the NRL balloons.
    Language English
    Publishing date 2016-01-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2661116-8
    ISSN 2193-1801
    ISSN 2193-1801
    DOI 10.1186/s40064-015-1660-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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