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  1. Article ; Online: Pulling instead of pushing: A case report of gastrostomy-assisted pull technique as an alternative method for endoluminal sponge placement in EVAC therapy.

    Trocchia, Carolena / Shieh, Hester F / Dolan, Isabella / Wilsey, Michael / Smithers, Charles J

    JPGN reports

    2024  Volume 5, Issue 1, Page(s) 74–78

    Abstract: Endoscopic vacuum-assisted therapy offers an easier and safer alternative to thoracic surgery, self-expanding stents, or esophageal clips and has been shown to be a promising technique for management of pediatric esophageal perforations. In this report, ... ...

    Abstract Endoscopic vacuum-assisted therapy offers an easier and safer alternative to thoracic surgery, self-expanding stents, or esophageal clips and has been shown to be a promising technique for management of pediatric esophageal perforations. In this report, we present a novel application of a percutaneous endoscopic gastrostomy-assisted pull technique, wherein a preexisting gastrostomy is reaccessed to allow safe placement of the vacuum sponge with a more comfortable and effective endoscopic vacuum-assisted closure therapy compared to transnasal or transoral options. A 7-year-old male with a history of type C esophageal atresia with distal tracheoesophageal fistula complicated by leak and refractory esophageal stricture, severe tracheomalacia, and prior esophageal stricture resection presented for posterior tracheoplasty and tracheopexy complicated by esophageal perforation. A preexisting gastrostomy site was re-accessed to allow for a novel approach for endoluminal sponge placement in endoscopic vacuum-assisted closure (EVAC) therapy by gastrostomy-assisted pull technique. The patient had appropriate healing without further leak 1 month after repair. This case highlights the use of EVAC as a minimally invasive option for repair of esophageal perforation using a pull-through method at the percutaneous endoscopic gastrostomy tube site as gastric access. This method may improve control of placement and reduce sponge migration, reduce intraluminal distance of sponge placement, and reduce morbidity by avoiding thoracotomy.
    Language English
    Publishing date 2024-01-18
    Publishing country United States
    Document type Case Reports
    ISSN 2691-171X
    ISSN (online) 2691-171X
    DOI 10.1002/jpr3.12040
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: High-throughput screening of the Saccharomyces cerevisiae genome for 2-amino-3-methylimidazo [4,5-f] quinoline resistance identifies colon cancer-associated genes.

    Dolan, Michael / St John, Nick / Zaidi, Faizan / Doyle, Francis / Fasullo, Michael

    G3 (Bethesda, Md.)

    2023  Volume 13, Issue 12

    Abstract: ... budding yeast) to identify genes that confer resistance to 2-amino-3-methylimidazo[4,5-f] quinoline (IQ). CYP1A2 ...

    Abstract Heterocyclic aromatic amines (HAAs) are potent carcinogenic agents found in charred meats and cigarette smoke. However, few eukaryotic resistance genes have been identified. We used Saccharomyces cerevisiae (budding yeast) to identify genes that confer resistance to 2-amino-3-methylimidazo[4,5-f] quinoline (IQ). CYP1A2 and NAT2 activate IQ to become a mutagenic nitrenium compound. Deletion libraries expressing human CYP1A2 and NAT2 or no human genes were exposed to either 400 or 800 µM IQ for 5 or 10 generations. DNA barcodes were sequenced using the Illumina HiSeq 2500 platform and statistical significance was determined for exactly matched barcodes. We identified 424 ORFs, including 337 genes of known function, in duplicate screens of the "humanized" collection for IQ resistance; resistance was further validated for a select group of 51 genes by growth curves, competitive growth, or trypan blue assays. Screens of the library not expressing human genes identified 143 ORFs conferring resistance to IQ per se. Ribosomal protein and protein modification genes were identified as IQ resistance genes in both the original and "humanized" libraries, while nitrogen metabolism, DNA repair, and growth control genes were also prominent in the "humanized" library. Protein complexes identified included the casein kinase 2 (CK2) and histone chaperone (HIR) complex. Among DNA Repair and checkpoint genes, we identified those that function in postreplication repair (RAD18, UBC13, REV7), base excision repair (NTG1), and checkpoint signaling (CHK1, PSY2). These studies underscore the role of ribosomal protein genes in conferring IQ resistance, and illuminate DNA repair pathways for conferring resistance to activated IQ.
    MeSH term(s) Humans ; Cytochrome P-450 CYP1A2/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; High-Throughput Screening Assays ; Early Detection of Cancer ; Mutagens ; Quinolines/pharmacology ; Quinolines/metabolism ; Ribosomal Proteins ; Colonic Neoplasms ; Arylamine N-Acetyltransferase/genetics ; DNA-Binding Proteins ; Ubiquitin-Protein Ligases ; DNA-Directed DNA Polymerase
    Chemical Substances Cytochrome P-450 CYP1A2 (EC 1.14.14.1) ; Mutagens ; Quinolines ; Ribosomal Proteins ; NAT2 protein, human (EC 2.3.1.5) ; Arylamine N-Acetyltransferase (EC 2.3.1.5) ; RAD18 protein, human ; DNA-Binding Proteins ; Ubiquitin-Protein Ligases (EC 2.3.2.27) ; REV7 protein, S cerevisiae ; DNA-Directed DNA Polymerase (EC 2.7.7.7) ; UBC13 protein, S cerevisiae (EC 2.3.2.23)
    Language English
    Publishing date 2023-09-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 2629978-1
    ISSN 2160-1836 ; 2160-1836
    ISSN (online) 2160-1836
    ISSN 2160-1836
    DOI 10.1093/g3journal/jkad219
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: In Vitro Glycosylation of the Membrane Protein γ-Sarcoglycan in Nanodiscs.

    Harris, Michael S / Dolan, Rachel F / Bryce, James R / Ewusi, Jonas G / Cook, Gabriel A

    ACS omega

    2023  Volume 8, Issue 43, Page(s) 40904–40910

    Abstract: Membrane glycoproteins are proteins that reside in the membranes of cells and are post-translationally modified to have sugars attached to their amino acid side chains. Studies of this subset of proteins in their native states are becoming more important ...

    Abstract Membrane glycoproteins are proteins that reside in the membranes of cells and are post-translationally modified to have sugars attached to their amino acid side chains. Studies of this subset of proteins in their native states are becoming more important since they have been linked to numerous human diseases. However, these proteins are difficult to study due to their hydrophobic nature and their propensity to aggregate. Using membrane mimetics allows us to solubilize these proteins, which, in turn, allows us to perform glycosylation
    Language English
    Publishing date 2023-10-20
    Publishing country United States
    Document type Journal Article
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.3c06135
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: In Vitro Glycosylation of the Membrane Protein γ‑Sarcoglycan in Nanodiscs

    Michael S. Harris / Rachel F. Dolan / James R. Bryce / Jonas G. Ewusi / Gabriel A. Cook

    ACS Omega, Vol 8, Iss 43, Pp 40904-

    2023  Volume 40910

    Keywords Chemistry ; QD1-999
    Language English
    Publishing date 2023-10-01T00:00:00Z
    Publisher American Chemical Society
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: In memoriam: Lynn Margulis (1938-2011).

    Dolan, Michael F

    The Journal of eukaryotic microbiology

    2012  Volume 59, Issue 4, Page(s) 427–428

    MeSH term(s) Eukaryota/classification ; Eukaryota/physiology ; History, 20th Century ; History, 21st Century ; Symbiosis
    Language English
    Publishing date 2012-07
    Publishing country United States
    Document type Biography ; Historical Article ; Journal Article
    ZDB-ID 1147218-2
    ISSN 1550-7408 ; 1066-5234
    ISSN (online) 1550-7408
    ISSN 1066-5234
    DOI 10.1111/j.1550-7408.2012.00622.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Case report:

    Balik, Sarah Emily / Ossiboff, Robert James / Stacy, Nicole Indra / Wellehan, James F X / Huguet, Elodie E / Gallastegui, Aitor / Childress, April L / Baldrica, Brittany E / Dolan, Brittany A / Adler, Laurie E / Walsh, Michael Thomas

    Frontiers in veterinary science

    2023  Volume 10, Page(s) 1132161

    Abstract: A complete postmortem examination, including a computed tomography scan "virtopsy" (virtual necropsy), gross necropsy, cytology, histology, and molecular diagnostics were performed to investigate the cause of death of a deceased adult male Atlantic ... ...

    Abstract A complete postmortem examination, including a computed tomography scan "virtopsy" (virtual necropsy), gross necropsy, cytology, histology, and molecular diagnostics were performed to investigate the cause of death of a deceased adult male Atlantic spotted dolphin (
    Language English
    Publishing date 2023-04-03
    Publishing country Switzerland
    Document type Case Reports
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2023.1132161
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Using Instrumental Variables to Measure Causation over Time in Cross-Lagged Panel Models.

    Singh, Madhurbain / Verhulst, Brad / Vinh, Philip / Zhou, Yi Daniel / Castro-de-Araujo, Luis F S / Hottenga, Jouke-Jan / Pool, René / de Geus, Eco J C / Vink, Jacqueline M / Boomsma, Dorret I / Maes, Hermine H M / Dolan, Conor V / Neale, Michael C

    Multivariate behavioral research

    2024  Volume 59, Issue 2, Page(s) 342–370

    Abstract: Cross-lagged panel models (CLPMs) are commonly used to estimate causal influences between two variables with repeated assessments. The lagged effects in a CLPM depend on the time interval between assessments, eventually becoming undetectable at longer ... ...

    Abstract Cross-lagged panel models (CLPMs) are commonly used to estimate causal influences between two variables with repeated assessments. The lagged effects in a CLPM depend on the time interval between assessments, eventually becoming undetectable at longer intervals. To address this limitation, we incorporate instrumental variables (IVs) into the CLPM with two study waves and two variables. Doing so enables estimation of both the lagged (i.e., "distal") effects and the bidirectional cross-sectional (i.e., "proximal") effects at each wave. The distal effects reflect Granger-causal influences across time, which decay with increasing time intervals. The proximal effects capture causal influences that accrue over time and can help infer causality when the distal effects become undetectable at longer intervals. Significant proximal effects, with a negligible distal effect, would imply that the time interval is too long to estimate a lagged effect at that time interval using the standard CLPM. Through simulations and an empirical application, we demonstrate the impact of time intervals on causal inference in the CLPM and present modeling strategies to detect causal influences regardless of the time interval in a study. Furthermore, to motivate empirical applications of the proposed model, we highlight the utility and limitations of using genetic variables as IVs in large-scale panel studies.
    MeSH term(s) Models, Statistical ; Cross-Sectional Studies ; Causality
    Language English
    Publishing date 2024-02-15
    Publishing country United States
    Document type Journal Article
    ISSN 1532-7906
    ISSN (online) 1532-7906
    DOI 10.1080/00273171.2023.2283634
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: MR-DoC2: Bidirectional Causal Modeling with Instrumental Variables and Data from Relatives.

    Castro-de-Araujo, Luis F S / Singh, Madhurbain / Zhou, Yi / Vinh, Philip / Verhulst, Brad / Dolan, Conor V / Neale, Michael C

    Behavior genetics

    2022  Volume 53, Issue 1, Page(s) 63–73

    Abstract: Establishing causality is an essential step towards developing interventions for psychiatric disorders, substance use and many other conditions. While randomized controlled trials (RCTs) are considered the gold standard for causal inference, they are ... ...

    Abstract Establishing causality is an essential step towards developing interventions for psychiatric disorders, substance use and many other conditions. While randomized controlled trials (RCTs) are considered the gold standard for causal inference, they are unethical in many scenarios. Mendelian randomization (MR) can be used in such cases, but importantly both RCTs and MR assume unidirectional causality. In this paper, we developed a new model, MRDoC2, that can be used to identify bidirectional causation in the presence of confounding due to both familial and non-familial sources. Our model extends the MRDoC model (Minică et al. in Behav Genet 48:337-349,  https://doi.org/10.1007/s10519-018-9904-4 , 2018), by simultaneously including risk scores for each trait. Furthermore, the power to detect causal effects in MRDoC2 does not require the phenotypes to have different additive genetic or shared environmental sources of variance, as is the case in the direction of causation twin model (Heath et al. in Behav Genet 23:29-50,  https://doi.org/10.1007/BF01067552 , 1993).
    MeSH term(s) Humans ; Risk Factors ; Causality ; Phenotype ; Mental Disorders ; Genome-Wide Association Study
    Language English
    Publishing date 2022-11-02
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 280238-7
    ISSN 1573-3297 ; 0005-7851 ; 0001-8244
    ISSN (online) 1573-3297
    ISSN 0005-7851 ; 0001-8244
    DOI 10.1007/s10519-022-10122-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Self-reported childhood family adversity is linked to an attenuated gain of trust during adolescence.

    Reiter, Andrea M F / Hula, Andreas / Vanes, Lucy / Hauser, Tobias U / Kokorikou, Danae / Goodyer, Ian M / Fonagy, Peter / Moutoussis, Michael / Dolan, Raymond J

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 6920

    Abstract: A longstanding proposal in developmental research is that childhood family experiences provide a template that shapes a capacity for trust-based social relationships. We leveraged longitudinal data from a cohort of healthy adolescents (n = 570, aged 14- ... ...

    Abstract A longstanding proposal in developmental research is that childhood family experiences provide a template that shapes a capacity for trust-based social relationships. We leveraged longitudinal data from a cohort of healthy adolescents (n = 570, aged 14-25), which included decision-making and psychometric data, to characterise normative developmental trajectories of trust behaviour and inter-individual differences therein. Extending on previous cross-sectional findings from the same cohort, we show that a task-based measure of trust increases longitudinally from adolescence into young adulthood. Computational modelling suggests this is due to a decrease in social risk aversion. Self-reported family adversity attenuates this developmental gain in trust behaviour, and within our computational model, this relates to a higher 'irritability' parameter in those reporting greater adversity. Unconditional trust at measurement time point T1 predicts the longitudinal trajectory of self-reported peer relation quality, particularly so for those with higher family adversity, consistent with trust acting as a resilience factor.
    MeSH term(s) Humans ; Child ; Adolescent ; Young Adult ; Adult ; Self Report ; Trust ; Cross-Sectional Studies ; Longitudinal Studies ; Interpersonal Relations
    Language English
    Publishing date 2023-10-30
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-41531-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Self-reported childhood family adversity is linked to an attenuated gain of trust during adolescence

    Andrea M. F. Reiter / Andreas Hula / Lucy Vanes / Tobias U. Hauser / Danae Kokorikou / Ian M. Goodyer / NSPN Consortium / Peter Fonagy / Michael Moutoussis / Raymond J. Dolan

    Nature Communications, Vol 14, Iss 1, Pp 1-

    2023  Volume 14

    Abstract: Abstract A longstanding proposal in developmental research is that childhood family experiences provide a template that shapes a capacity for trust-based social relationships. We leveraged longitudinal data from a cohort of healthy adolescents (n = 570, ... ...

    Abstract Abstract A longstanding proposal in developmental research is that childhood family experiences provide a template that shapes a capacity for trust-based social relationships. We leveraged longitudinal data from a cohort of healthy adolescents (n = 570, aged 14–25), which included decision-making and psychometric data, to characterise normative developmental trajectories of trust behaviour and inter-individual differences therein. Extending on previous cross-sectional findings from the same cohort, we show that a task-based measure of trust increases longitudinally from adolescence into young adulthood. Computational modelling suggests this is due to a decrease in social risk aversion. Self-reported family adversity attenuates this developmental gain in trust behaviour, and within our computational model, this relates to a higher ‘irritability’ parameter in those reporting greater adversity. Unconditional trust at measurement time point T1 predicts the longitudinal trajectory of self-reported peer relation quality, particularly so for those with higher family adversity, consistent with trust acting as a resilience factor.
    Keywords Science ; Q
    Language English
    Publishing date 2023-10-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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