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  1. Article ; Online: Digital Forensics and Computer Crimes

    Imeri-Saiti Deshira / Hamiti Mentor

    SEEU Review, Vol 18, Iss 2, Pp 55-

    The Case of North Macedonia

    2023  Volume 73

    Abstract: The subject of the research refers to the aspect of digital forensics and computer crime and the latter is one of the reasons for the evolution of crime in general. Based on the growing trend of technology development and the increase in the number of ... ...

    Abstract The subject of the research refers to the aspect of digital forensics and computer crime and the latter is one of the reasons for the evolution of crime in general. Based on the growing trend of technology development and the increase in the number of digital crimes, a special emphasis is given to the statistical aspect of computer crime as well as measures to reduce the impact of computer crime, including the ethical and legal aspects in the Republic of North Macedonia (RNM).
    Keywords digital forensic ; computer crime ; ethics ; law ; north macedonia ; K ; Political science (General) ; JA1-92
    Language English
    Publishing date 2023-12-01T00:00:00Z
    Publisher Sciendo
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Density gradients for the isolation of germ cells from embryoid bodies.

    Saiti, Deshira / Lacham-Kaplan, Orly

    Reproductive biomedicine online

    2008  Volume 16, Issue 5, Page(s) 730–740

    Abstract: In previous reports, the isolation of embryonic stem cell (ESC)-derived germ cells utilized fluorescent protein knock-in cell lines that could be sorted by flow cytometry. The present study aimed to isolate putative germ stem cells from embryoid bodies ( ... ...

    Abstract In previous reports, the isolation of embryonic stem cell (ESC)-derived germ cells utilized fluorescent protein knock-in cell lines that could be sorted by flow cytometry. The present study aimed to isolate putative germ stem cells from embryoid bodies (EB) using Percoll and Nycodenz density gradients. The optimal ESC concentration to establish EB was identified as 15,000 cells per 30 mul drop and the optimal culture time to obtain the highest number of germ cells within EB was identified as 120 h, with over 25% of cells confirmed as germ cells for the specific cell line used. Germ cells were isolated from 120-hourold EB by Percoll and Nycodenz density gradients, while isolation of primordial germ cells from genital ridges of embryonic day 12.5 fetuses was used as a control. Putative germ cells were isolated from EB at proportions of 80.3 +/- 3.0% (SEM) and 75.8 +/- 0.9% for Percoll and Nycodenz respectively. Primordial germ cells were isolated from genital ridges at rates of 89.7 +/- 2.7% and 89.5 +/- 0.9% respectively. Although the purity of the isolated germ cells was similar between the two gradients, more germ cells with higher viability were obtained with the Percoll gradient.
    MeSH term(s) Cell Separation ; Embryo, Mammalian/cytology ; Germ Cells/cytology ; Humans
    Language English
    Publishing date 2008-05-05
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2113823-0
    ISSN 1472-6491 ; 1472-6483
    ISSN (online) 1472-6491
    ISSN 1472-6483
    DOI 10.1016/s1472-6483(10)60489-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Mouse Germ Cell Development in-vivo and in-vitro.

    Saiti, Deshira / Lacham-Kaplan, Orly

    Biomarker insights

    2007  Volume 2, Page(s) 241–252

    Abstract: In mammalian development, primordial germ cells (PGCs) represent the initial population of cells that are committed to the germ cell lineage. PGCs segregate early in development, triggered by signals from the extra-embryonic ectoderm. They are ... ...

    Abstract In mammalian development, primordial germ cells (PGCs) represent the initial population of cells that are committed to the germ cell lineage. PGCs segregate early in development, triggered by signals from the extra-embryonic ectoderm. They are distinguished from surrounding cells by their unique gene expression patterns. Some of the more common genes used to identify them are Blimp1, Oct3/4, Fragilis, Stella, c-Kit, Mvh, Dazl and Gcna1. These genes are involved in regulating their migration and differentiation, and in maintaining the pluripotency of these cells.Recent research has demonstrated the possibility of obtaining PGCs, and subsequently, mature germ cells from a starting population of embryonic stem cells (ESCs) in culture. This phenomenon has been investigated using a variety of methods, and ESC lines of both mouse and human origin. Embryonic stem cells can differentiate into germ cells of both the male and female phenotype and in one case has resulted in the birth of live pups from the fertilization of oocytes with ESC derived sperm. This finding leads to the prospect of using ESC derived germ cells as a treatment for sterility. This review outlines the evolvement of germ cells from ESCs in vitro in relation to in vivo events.
    Language English
    Publishing date 2007-06-06
    Publishing country United States
    Document type Journal Article
    ISSN 1177-2719
    ISSN 1177-2719
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Mouse Germ Cell Development in-vivo and in-vitro

    Deshira Saiti / Orly Lacham-Kaplan

    Biomarker Insights, Vol 2, Pp 241-

    2007  Volume 252

    Abstract: In mammalian development, primordial germ cells (PGCs) represent the initial population of cells that are committed to the germ cell lineage. PGCs segregate early in development, triggered by signals from the extra-embryonic ectoderm. They are ... ...

    Abstract In mammalian development, primordial germ cells (PGCs) represent the initial population of cells that are committed to the germ cell lineage. PGCs segregate early in development, triggered by signals from the extra-embryonic ectoderm. They are distinguished from surrounding cells by their unique gene expression patterns. Some of the more common genes used to identify them are Blimp1, Oct3/4, Fragilis, Stella, c-Kit, Mvh, Dazl and Gcna1. These genes are involved in regulating their migration and differentiation, and in maintaining the pluripotency of these cells. Recent research has demonstrated the possibility of obtaining PGCs, and subsequently, mature germ cells from a starting population of embryonic stem cells (ESCs) in culture. This phenomenon has been investigated using a variety of methods, and ESC lines of both mouse and human origin. Embryonic stem cells can differentiate into germ cells of both the male and female phenotype and in one case has resulted in the birth of live pups from the fertilization of oocytes with ESC derived sperm. This fi nding leads to the prospect of using ESC derived germ cells as a treatment for sterility. This review outlines the evolvement of germ cells from ESCs in vitro in relation to in vivo events.
    Keywords Medicine (General) ; R5-920
    Subject code 570
    Language English
    Publishing date 2007-01-01T00:00:00Z
    Publisher SAGE Publishing
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: In vitro investigation of renal epithelial injury suggests that primary cilium length is regulated by hypoxia-inducible mechanisms.

    Verghese, Elizabeth / Zhuang, Junli / Saiti, Deshira / Ricardo, Sharon D / Deane, James A

    Cell biology international

    2011  Volume 35, Issue 9, Page(s) 909–913

    Abstract: Primary cilia are non-motile sensory organelles that project from cells in many tissues. The role of renal primary cilium-based signalling in regulating epithelial cell proliferation and differentiation is highlighted by studies showing that defects of ... ...

    Abstract Primary cilia are non-motile sensory organelles that project from cells in many tissues. The role of renal primary cilium-based signalling in regulating epithelial cell proliferation and differentiation is highlighted by studies showing that defects of the cilium lead to epithelial de-differentiation, over proliferation and polycystic kidney disease. Recent studies show that renal primary cilia may also play a role in controlling epithelial differentiation during renal repair. After injury, renal cilium length increases dramatically and then undergoes a normalization that coincides with structural and functional repair in both human patients and mouse models of renal injury. These changes in cilium length are likely to modulate cilium-based signalling, but the injury-related factors that influence renal primary cilium length have yet to be determined. Here, we investigated the effect of three factors commonly associated with renal injury on renal cilium length in an in vitro setting. MDCK (Madin Darby canine kidney) cell cultures bearing primary cilia were treated with BSA to simulate albuminuria, cobalt chloride to simulate hypoxia and the inflammation-related cytokine tumour necrosis factor α. Primary cilium length was only increased in cultures treated with cobalt chloride. Our results suggest a role for hypoxia and the induction of HIF-1α (hypoxia-inducible factor 1α) in increasing renal primary cilium length following renal injury.
    MeSH term(s) Animals ; Cell Hypoxia ; Cells, Cultured ; Cilia/physiology ; Cilia/ultrastructure ; Dogs ; Epithelial Cells/pathology ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism ; Kidney/pathology ; Kidney/ultrastructure
    Chemical Substances Hypoxia-Inducible Factor 1, alpha Subunit
    Language English
    Publishing date 2011-09
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1143453-3
    ISSN 1095-8355 ; 1065-6995
    ISSN (online) 1095-8355
    ISSN 1065-6995
    DOI 10.1042/CBI20090154
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1451: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
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    ab Jg. 2022: Lesesaal (EG)

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