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  1. Article ; Online: Structural Impact of Steroidal Glycoalkaloids: Barrier Integrity, Permeability, Metabolism, and Uptake in Intestinal Cells.

    Keuter, Lucas / Wolbeck, Alessa / Kasimir, Matthias / Schürmann, Lina / Behrens, Matthias / Humpf, Hans-Ulrich

    Molecular nutrition & food research

    2024  Volume 68, Issue 6, Page(s) e2300639

    Abstract: Scope: Potato tubers represent an essential food component all over the world and an important supplier of carbohydrates, fiber, and valuable proteins. However, besides their health promoting effects, potatoes contain α-solanine and α-chaconine, which ... ...

    Abstract Scope: Potato tubers represent an essential food component all over the world and an important supplier of carbohydrates, fiber, and valuable proteins. However, besides their health promoting effects, potatoes contain α-solanine and α-chaconine, which are toxic steroidal glycoalkaloids (SGAs). Other solanaceous plants like eggplants and tomatoes produce SGAs as well, different in their chemical structure. This study aims to investigate toxic effects (cholinesterase inhibition, membrane, and barrier disruption), permeability, metabolism, and structure-activity relationships of SGAs.
    Methods and results: α-solanine, α-chaconine, α-solasonine, α-solamargine, α-tomatine, and their respective aglycones solanidine, solasodine, and tomatidine are analyzed using Ellman assay, cellular impedance spectroscopy, cell extraction, and Caco-2 intestinal model. Additionally, metabolism is analyzed by HPLC-MS techniques. The study observes dependencies of barrier disrupting potential and cellular uptake on the carbohydrate moiety of SGAs, while permeability and acetylcholinesterase (AChE) inhibition are dominated by the steroid backbone. SGAs show low permeabilities across Caco-2 monolayers in subtoxic concentrations. In contrast, their respective aglycones reveal higher permeabilities, but are extensively metabolized.
    Conclusion: Besides structure-activity relationships, this study provides new information on the overall effects of steroidal alkaloids on intestinal cells and closes a gap of knowledge for the metabolic pathway from oral uptake to final excretion.
    MeSH term(s) Humans ; Acetylcholinesterase ; Caco-2 Cells ; Alkaloids/pharmacology ; Alkaloids/chemistry ; Structure-Activity Relationship ; Solanum tuberosum ; Permeability
    Chemical Substances Acetylcholinesterase (EC 3.1.1.7) ; Alkaloids
    Language English
    Publishing date 2024-02-22
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2160372-8
    ISSN 1613-4133 ; 1613-4125
    ISSN (online) 1613-4133
    ISSN 1613-4125
    DOI 10.1002/mnfr.202300639
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  2. Article ; Online: Characterization of non-invasive oropharyngeal samples and nucleic acid isolation for molecular diagnostics.

    Hose, Leonie / Schürmann, Matthias / Mennebröcker, Inga / Kim, Rayoung / Busche, Tobias / Goon, Peter / Sudhoff, Holger

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 4061

    Abstract: Molecular diagnostics is an increasingly important clinical tool, especially in routine sampling. We evaluated two non-invasive methods (oral swabs and mouthwashes) for sampling nucleic acids from the oral/pharyngeal area. We created a workflow from ... ...

    Abstract Molecular diagnostics is an increasingly important clinical tool, especially in routine sampling. We evaluated two non-invasive methods (oral swabs and mouthwashes) for sampling nucleic acids from the oral/pharyngeal area. We created a workflow from sample collection (n = 59) to RT-qPCR based analysis. The samples were further characterized in terms of their cellular composition as well as the purity, degradation and microbial content of the derived DNA/RNA. We determined the optimal housekeeping genes applicable for these types of samples. The cellular composition indicated that mouthwashes contained more immune cells and bacteria. Even though the protocol was not specifically optimized to extract bacterial RNA it was possible to derive microbial RNA, from both sampling methods. Optimizing the protocol allowed us to generate stable quantities of DNA/RNA. DNA/RNA purity parameters were not significantly different between the two sampling methods. Even though integrity analysis demonstrated a high level of degradation of RNA, corresponding parameters confirmed their sequencing potential. RT-qPCR analysis determined TATA-Box Binding Protein as the most favorable housekeeping gene. In summary, we have developed a robust method suitable for multiple downstream diagnostic techniques. This protocol can be used as a foundation for further research endeavors focusing on developing molecular diagnostics for the oropharyngeal cavity.
    MeSH term(s) Nucleic Acids/genetics ; Mouthwashes ; Pathology, Molecular ; RNA/genetics ; DNA
    Chemical Substances Nucleic Acids ; Mouthwashes ; RNA (63231-63-0) ; DNA (9007-49-2)
    Language English
    Publishing date 2024-02-19
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-54179-6
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  3. Article ; Conference proceedings: "Microscopic characterization of tissues in middle ear/cholesteatoma surgery using shortwave infrared (SWIR)"

    Rempen, Alexander / Huser, Thomas / Schürmann, Matthias / Sudhoff, Holger

    Laryngo-Rhino-Otologie

    2023  Volume 102, Issue S 02

    Event/congress 94th Annual Meeting German Society of Oto-Rhino-Laryngology, Head and Neck Surgery e.V., Bonn, Congress Center Leipzig, 2023-05-17
    Language English
    Publishing date 2023-05-01
    Publisher Georg Thieme Verlag
    Publishing place Stuttgart ; New York
    Document type Article ; Conference proceedings
    ZDB-ID 96005-6
    ISSN 1438-8685 ; 0935-8943 ; 0340-1588
    ISSN (online) 1438-8685
    ISSN 0935-8943 ; 0340-1588
    DOI 10.1055/s-0043-1767111
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  4. Article ; Conference proceedings: ,,Mikroskopische Charakterisierung von Geweben in der Mittelohr-/Cholesteatomchirurgie mittels Kurzwellen-Infrarot (SWIR)“

    Rempen, Alexander / Huser, Thomas / Schürmann, Matthias / Sudhoff, Holger

    Laryngo-Rhino-Otologie

    2023  Volume 102, Issue S 02

    Event/congress 94. Jahresversammlung Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e.V., Bonn, Congress Center Leipzig, 2023-05-17
    Language German
    Publishing date 2023-05-01
    Publisher Georg Thieme Verlag
    Publishing place Stuttgart ; New York
    Document type Article ; Conference proceedings
    ZDB-ID 96005-6
    ISSN 1438-8685 ; 0935-8943 ; 0340-1588
    ISSN (online) 1438-8685
    ISSN 0935-8943 ; 0340-1588
    DOI 10.1055/s-0043-1766518
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  5. Article ; Online: Review of potential medical treatments for middle ear cholesteatoma.

    Schürmann, Matthias / Goon, Peter / Sudhoff, Holger

    Cell communication and signaling : CCS

    2022  Volume 20, Issue 1, Page(s) 148

    Abstract: Middle ear cholesteatoma (MEC), is a destructive, and locally invasive lesion in the middle ear driven by inflammation with an annual incidence of 10 per 100,000. Surgical extraction/excision remains the only treatment strategy available and recurrence ... ...

    Abstract Middle ear cholesteatoma (MEC), is a destructive, and locally invasive lesion in the middle ear driven by inflammation with an annual incidence of 10 per 100,000. Surgical extraction/excision remains the only treatment strategy available and recurrence is high (up to 40%), therefore developing the first pharmaceutical treatments for MEC is desperately required. This review was targeted at connecting the dysregulated inflammatory network of MEC to pathogenesis and identification of pharmaceutical targets. We summarized the numerous basic research endeavors undertaken over the last 30+ years to identify the key targets in the dysregulated inflammatory pathways and judged the level of evidence for a given target if it was generated by in vitro, in vivo or clinical experiments. MEC pathogenesis was found to be connected to cytokines characteristic for Th1, Th17 and M1 cells. In addition, we found that the inflammation created damage associated molecular patterns (DAMPs), which further promoted inflammation. Similar positive feedback loops have already been described for other Th1/Th17 driven inflammatory diseases (arthritis, Crohn's disease or multiple sclerosis). A wide-ranging search for molecular targeted therapies (MTT) led to the discovery of over a hundred clinically approved drugs already applied in precision medicine. Based on exclusion criteria designed to enable fast translation as well as efficacy, we condensed the numerous MTTs down to 13 top drugs. The review should serve as groundwork for the primary goal, which is to provide potential pharmaceutical therapies to MEC patients for the first time in history. Video Abstract.
    MeSH term(s) Cholesteatoma, Middle Ear/metabolism ; Cholesteatoma, Middle Ear/pathology ; Cholesteatoma, Middle Ear/surgery ; Cytokines ; Ear, Middle/metabolism ; Ear, Middle/pathology ; Humans ; Inflammation/pathology
    Chemical Substances Cytokines
    Language English
    Publishing date 2022-09-19
    Publishing country England
    Document type Journal Article ; Review ; Video-Audio Media ; Research Support, Non-U.S. Gov't
    ZDB-ID 2126315-2
    ISSN 1478-811X ; 1478-811X
    ISSN (online) 1478-811X
    ISSN 1478-811X
    DOI 10.1186/s12964-022-00953-w
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  6. Book ; Thesis: Lungenembolien im chirurgischen Krankengut

    Schürmann, Matthias

    eine retrospektive Studie über 9 Jahre

    1991  

    Author's details vorgelegt von Matthias Schürmann
    Size 105 S. : Ill., graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis München, Univ., Diss., 1991
    HBZ-ID HT004286133
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    92 D 3478 order for loan Magazin

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  7. Article ; Online: Bayesian random-effects meta-analysis with empirical heterogeneity priors for application in health technology assessment with very few studies.

    Lilienthal, Jona / Sturtz, Sibylle / Schürmann, Christoph / Maiworm, Matthias / Röver, Christian / Friede, Tim / Bender, Ralf

    Research synthesis methods

    2023  Volume 15, Issue 2, Page(s) 275–287

    Abstract: In Bayesian random-effects meta-analysis, the use of weakly informative prior distributions is of particular benefit in cases where only a few studies are included, a situation often encountered in health technology assessment (HTA). Suggestions for ... ...

    Abstract In Bayesian random-effects meta-analysis, the use of weakly informative prior distributions is of particular benefit in cases where only a few studies are included, a situation often encountered in health technology assessment (HTA). Suggestions for empirical prior distributions are available in the literature but it is unknown whether these are adequate in the context of HTA. Therefore, a database of all relevant meta-analyses conducted by the Institute for Quality and Efficiency in Health Care (IQWiG, Germany) was constructed to derive empirical prior distributions for the heterogeneity parameter suitable for HTA. Previously, an extension to the normal-normal hierarchical model had been suggested for this purpose. For different effect measures, this extended model was applied on the database to conservatively derive a prior distribution for the heterogeneity parameter. Comparison of a Bayesian approach using the derived priors with IQWiG's current standard approach for evidence synthesis shows favorable properties. Therefore, these prior distributions are recommended for future meta-analyses in HTA settings and could be embedded into the IQWiG evidence synthesis approach in the case of very few studies.
    MeSH term(s) Bayes Theorem ; Technology Assessment, Biomedical ; Databases, Factual ; Germany ; Information Dissemination
    Language English
    Publishing date 2023-12-28
    Publishing country England
    Document type Meta-Analysis ; Journal Article
    ZDB-ID 2548499-0
    ISSN 1759-2887 ; 1759-2879
    ISSN (online) 1759-2887
    ISSN 1759-2879
    DOI 10.1002/jrsm.1685
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  8. Article ; Online: Mouthrinses against SARS-CoV-2: anti-inflammatory effectivity and a clinical pilot study.

    Schürmann, Matthias / Aljubeh, Mohamed / Tiemann, Carsten / Sudhoff, Holger

    European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery

    2021  Volume 278, Issue 12, Page(s) 5059–5067

    Abstract: Purpose: The scope of this research endeavor was the determination of the applicability of over the counter mouthwash solutions in reducing the viral load in the saliva of COVID-19 patients and hence decreasing their infectivity. Beyond that, new ... ...

    Abstract Purpose: The scope of this research endeavor was the determination of the applicability of over the counter mouthwash solutions in reducing the viral load in the saliva of COVID-19 patients and hence decreasing their infectivity. Beyond that, new experimental mouthwashes were investigated in terms of a possible positive immune modulation, which might offer an additional opportunity for a positive pharmaceutical effect.
    Methods: The effectivity of the mouth washing solution was determined on 34 hospitalized COVID-19 patients by measuring the viral load by RT-qPCR in pharyngeal swabs, which were taken before and after rinsing. The inflammatory modulation thru the experimental solutions was assayed in an in vitro model of virus infected nasopharyngeal epithelium cells.
    Results: The clinical pilot study demonstrated that the mouth rinsing solution was able to reduce the viral load by about 90% in the saliva of most patients. This reduction was determined to persist for about 6 h. In the experimental solutions, the ingredients dexpanthenol and zinc were able to reduce the expression of proinflammatory cytokines in the cell culture model, while the antiviral response was not altered significantly.
    Conclusion: We recommend the application of mouth wash solutions to COVID-19 patients, since our results indicate a reduction in infectivity and might govern the protection of health care professionals. Further improvement to the over the counter formulation can be made by utilizing zinc and dexpanthenol, as they which might be beneficial for the patients' health.
    MeSH term(s) Anti-Inflammatory Agents ; COVID-19 ; Humans ; Pilot Projects ; SARS-CoV-2 ; Saliva ; Viral Load
    Chemical Substances Anti-Inflammatory Agents
    Language English
    Publishing date 2021-05-22
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1017359-6
    ISSN 1434-4726 ; 0937-4477
    ISSN (online) 1434-4726
    ISSN 0937-4477
    DOI 10.1007/s00405-021-06873-8
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  9. Article ; Conference proceedings: Untersuchung von Korrelationen zwischen dem Auftreten von Symptomen eines Post-COVID-Syndroms nach SARS-CoV-2-Infektion, des cycle threshold in der RT-PCR, dem Lebensalter und der Hospitalisierung

    Rempen, Alexander / Pfeiffer, Christoph / Riemann, Conrad / Sudhoff, Holger / Scholtz, Lars-Uwe / Todt, Ingo / Schürmann, Matthias / Hose, Leonie

    Laryngo-Rhino-Otologie

    2024  Volume 103, Issue S 02

    Event/congress 95. Jahresversammlung Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V., Bonn, Messe Essen, 2024-05-08
    Language German
    Publishing date 2024-04-19
    Publisher Georg Thieme Verlag KG
    Publishing place Stuttgart ; New York
    Document type Article ; Conference proceedings
    ZDB-ID 96005-6
    ISSN 1438-8685 ; 0935-8943 ; 0340-1588
    ISSN (online) 1438-8685
    ISSN 0935-8943 ; 0340-1588
    DOI 10.1055/s-0044-1784059
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  10. Article ; Conference proceedings: Molecular biomarker identification in non-invasive material for OSCC – a dual-omics approach

    Hose, Leonie / Kim, Rayoung / Moermans, Nathalie / Sudhoff, Holger / Goon, Peter / Scholtz, Lars-Uwe / Todt, Ingo / Schürmann, Matthias

    Laryngo-Rhino-Otologie

    2024  Volume 103, Issue S 02

    Event/congress 95th Annual Meeting German Society of Oto-Rhino-Laryngology, Head and Neck Surgery e. V., Bonn, Messe Essen, 2024-05-08
    Language English
    Publishing date 2024-04-19
    Publisher Georg Thieme Verlag KG
    Publishing place Stuttgart ; New York
    Document type Article ; Conference proceedings
    ZDB-ID 96005-6
    ISSN 1438-8685 ; 0935-8943 ; 0340-1588
    ISSN (online) 1438-8685
    ISSN 0935-8943 ; 0340-1588
    DOI 10.1055/s-0044-1784745
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