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  1. Book ; Conference proceedings: 41. Arbeits- und Fortbildungstagung der DVG-Fachgruppe AVID

    Balzer, Jörg / Barth, Stefanie / Eisenberg, Tobias / Scuda, Nelly / Wernike, Kerstin

    Arbeitskreis für veterinärmedizinische Infektionsdiagnostik : Kloster Banz und online, 13. bis 15. September 2023

    2023  

    Title variant Einundvierzigste Arbeits- und Fortbildungstagung der DVG-Fachgruppe AVID, Arbeitskreis für Veterinärmedizinische Infektionsdiagnostik ; Tagung der DVG-Fachgruppe AVID, Schwerpunkt Virologie
    Institution Deutsche Veterinärmedizinische Gesellschaft
    Event/congress Deutsche Veterinärmedizinische Gesellschaft (41., 2023, BadStaffelstein-BanzOnline, Fachgruppe AVID)
    Author's details AVID Vorstand: Dr. J. Balzer / Dr. S. Barth / Prof. Dr. T. Eisenberg / Dr. N. Scuda / PD Dr. K. Wernike ; Veranstalter / Organisation: DVG Service GmbH (Gießen)
    Language German ; English
    Size 15 ungezählte Seiten, 87 Seiten, Illustration
    Publisher Verlag der DVG Service GmbH
    Publishing place Gießen
    Publishing country Germany
    Document type Book ; Conference proceedings
    Note Text teilweise deutsch, teilweise englisch
    HBZ-ID HT030641474
    ISBN 978-3-86345-680-1 ; 3-86345-680-7
    Database Catalogue ZB MED Nutrition, Environment, Agriculture

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  2. Article: Bovine Viral Diarrhea/Mucosal Disease-A Commentary of the Guest Editor.

    Wernike, Kerstin

    Vaccines

    2022  Volume 10, Issue 4

    Abstract: Bovine viral diarrhea (BVD) is one of the most significant cattle diseases worldwide, and control programs have been implemented in several countries [ ... ]. ...

    Abstract Bovine viral diarrhea (BVD) is one of the most significant cattle diseases worldwide, and control programs have been implemented in several countries [...].
    Language English
    Publishing date 2022-04-12
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines10040590
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book ; Online: WOAH Reference Laboratory Reports Activities

    Wernike, Kerstin

    Bovine viral diarrhoea: Activities in 2022

    2023  

    Keywords Text ; ddc:610
    Language English
    Publishing date 2023-03-09
    Publishing country de
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Desinfektionsverfahren bei den einzelnen Tierseuchen

    Wernike, Kerstin

    Bovine Virus Diarrhoe

    2023  

    Keywords Text ; ddc:630 ; FLI-Information -- Desinfektion -- Anzeigepflichtige Tierseuche -- Bovine Virus Diarrhoe -- BVD
    Language German
    Publishing date 2023-03-20
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Bovine Viral Diarrhea/Mucosal Disease—A Commentary of the Guest Editor

    Kerstin Wernike

    Vaccines, Vol 10, Iss 590, p

    2022  Volume 590

    Abstract: Bovine viral diarrhea (BVD) is one of the most significant cattle diseases worldwide, and control programs have been implemented in several countries [.] ...

    Abstract Bovine viral diarrhea (BVD) is one of the most significant cattle diseases worldwide, and control programs have been implemented in several countries [.]
    Keywords n/a ; Medicine ; R
    Language English
    Publishing date 2022-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Comparison of bovine viral diarrhea virus detection methods: Results of an international proficiency trial.

    Wernike, Kerstin / Beer, Martin

    Veterinary microbiology

    2024  Volume 290, Page(s) 109985

    Abstract: Bovine viral diarrhea virus (BVDV), one of the most important infectious cattle diseases globally, is being combated in multiple countries. The main source for virus transmission within herds and especially to unaffected cattle farms are life-long ... ...

    Abstract Bovine viral diarrhea virus (BVDV), one of the most important infectious cattle diseases globally, is being combated in multiple countries. The main source for virus transmission within herds and especially to unaffected cattle farms are life-long persistently infected (PI), immunotolerant animals. Therefore, the early identification of PI calves is a major pillar of disease control programs. In addition, rapid and reliable virus identification is necessary to confirm the causative agent in acute clinical cases. Here, we initiated an international interlaboratory proficiency trial in order to evaluate BVDV detection methods. Four ear notch samples and four sera were provided to the participating veterinary diagnostic laboratories (n = 40). Two of the ear notches and two sera contained BVDV and two ear notches and one serum were negative for pestiviruses. The remaining serum was positive for the ovine border disease virus (BDV). The sample panel was analyzed by an E
    MeSH term(s) Animals ; Cattle ; Antibodies, Viral ; Border disease virus ; Bovine Virus Diarrhea-Mucosal Disease ; Cattle Diseases ; Diarrhea/veterinary ; Diarrhea Virus 1, Bovine Viral ; Diarrhea Viruses, Bovine Viral ; Enzyme-Linked Immunosorbent Assay/veterinary ; Enzyme-Linked Immunosorbent Assay/methods ; Pestivirus ; Sheep ; Sheep Diseases ; Sheep, Domestic
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2024-01-11
    Publishing country Netherlands
    Document type Clinical Trial, Veterinary ; Journal Article
    ZDB-ID 753154-0
    ISSN 1873-2542 ; 0378-1135
    ISSN (online) 1873-2542
    ISSN 0378-1135
    DOI 10.1016/j.vetmic.2024.109985
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Bovine Viral Diarrhea/Mucosal Disease—A Commentary of the Guest Editor

    Wernike, Kerstin

    2022  

    Keywords Text ; ddc:630
    Language English
    Publishing date 2022-04-12
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Book ; Audio / Video ; Online: BVD und AHL – Was kommt diagnostisch auf uns zu?

    Wernike, Kerstin

    2021  

    Keywords Text ; ddc:570
    Language German
    Publishing date 2021-09-15
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article: "Fading out" - genomic epidemiology of the last persistently infected BVDV cattle in Germany.

    Wernike, Kerstin / Pfaff, Florian / Beer, Martin

    Frontiers in veterinary science

    2024  Volume 10, Page(s) 1339248

    Abstract: Bovine viral diarrhea virus (BVDV) is one of the most important cattle pathogens worldwide, causing major economic losses and animal welfare issues. Disease eradication programs have been implemented in several countries, including Germany where an ... ...

    Abstract Bovine viral diarrhea virus (BVDV) is one of the most important cattle pathogens worldwide, causing major economic losses and animal welfare issues. Disease eradication programs have been implemented in several countries, including Germany where an obligatory nationwide control program is in force since 2011. As molecular epidemiology has become an essential tool to understand the transmission dynamics and evolution of BVDV, 5' untranslated region (UTR) sequences are generated from viruses present in persistently infected animals since the beginning of the BVDV control program. Here, we report the results of the sequence-based subtyping of BVDV strains found from 2018 through 2022 in calves born in Germany. In 2018, 2019 and 2020, BVDV-1d and-1b were the dominant subtypes and cases were spread throughout the area that was not yet officially declared BVDV-free at that time. In addition, BVDV-1a, -1e, -1f and -1h could rarely be detected. From 2021 onwards, subtype 1d clearly took over the dominance, while the other subtypes could be gradually nearly eliminated from the cattle population. The eradication success not only results in a drastic reduction of cases, but also in a marked reduction of strain diversity. Interestingly, before vaccination has been banned in regions and farms with a disease-free status, two live-vaccine virus strains were repeatedly detected in ear tissue samples of newborn calves (
    Language English
    Publishing date 2024-01-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2023.1339248
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Comparison of bovine viral diarrhea virus detection methods

    Wernike, Kerstin / Beer, Martin

    Results of an international proficiency trial

    2024  

    Abstract: Bovine viral diarrhea virus (BVDV), one of the most important infectious cattle diseases globally, is being combated in multiple countries. The main source for virus transmission within herds and especially to unaffected cattle farms are life-long ... ...

    Abstract Bovine viral diarrhea virus (BVDV), one of the most important infectious cattle diseases globally, is being combated in multiple countries. The main source for virus transmission within herds and especially to unaffected cattle farms are life-long persistently infected (PI), immunotolerant animals. Therefore, the early identification of PI calves is a major pillar of disease control programs. In addition, rapid and reliable virus identification is necessary to confirm the causative agent in acute clinical cases. Here, we initiated an international interlaboratory proficiency trial in order to evaluate BVDV detection methods. Four ear notch samples and four sera were provided to the participating veterinary diagnostic laboratories (n=40). Two of the ear notches and two sera contained BVDV and two ear notches and one serum were negative for pestiviruses. The remaining serum was positive for the ovine border disease virus (BDV). The sample panel was analyzed by an ERNS-based ELISA for antigen detection, diverse real-time RT-PCR (RT-qPCR) assays and/or virus isolation. Occasionally, additional typing of the virus strains was performed by sequencing or specific antibody staining of the obtained cell culture isolates. While the antigen ELISA allowed reliable BVDV diagnostics, infectious virus could be isolated only in just under half of the attempts (43.33%). RT-qPCR enabled the sensitive detection of pestiviruses, though an impact of the extraction method on the resulting quantification cycle values was observed. In general, subsequent typing of the detected virus strains is required to differentiate BVDV from BDV infections. In conclusion, for BVDV identification in clinical cases or in the context of disease control, RT-qPCR methods or ERNS antigen ELISAs should be preferentially used.
    Keywords Text ; article ; ddc:570 ; bovine viral diarrhea -- pestivirus -- diagnostics -- antigen ELISA -- RT-qPCR -- proficiency testing
    Subject code 630
    Language English
    Publishing date 2024-01-11
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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