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  1. Article ; Online: A Severe Infection Caused by a White Colony-Producing Strain of

    Park, Se Yoon / Kim, Heejung / Lee, Yangsoon

    Annals of laboratory medicine

    2024  Volume 44, Issue 4, Page(s) 375–377

    MeSH term(s) Humans ; Clostridioides difficile/genetics ; Clostridium Infections/diagnosis
    Language English
    Publishing date 2024-03-07
    Publishing country Korea (South)
    Document type Letter
    ZDB-ID 2677441-0
    ISSN 2234-3814 ; 2234-3814
    ISSN (online) 2234-3814
    ISSN 2234-3814
    DOI 10.3343/alm.2023.0474
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Emerging of multidrug-resistant Pseudomonas guariconensis with bla

    Yoon, Choseok / Lee, Yangsoon

    Diagnostic microbiology and infectious disease

    2024  Volume 108, Issue 3, Page(s) 116182

    Abstract: This case presents the clinical and genomic aspects of a rare and multidrug-resistant Pseudomonas guariconensis isolate carrying ... ...

    Abstract This case presents the clinical and genomic aspects of a rare and multidrug-resistant Pseudomonas guariconensis isolate carrying bla
    MeSH term(s) Humans ; Female ; Middle Aged ; beta-Lactamases/genetics ; Bacteriuria/diagnosis ; DNA, Bacterial/genetics ; DNA, Bacterial/chemistry ; RNA, Ribosomal, 16S/genetics ; Agar ; Microbial Sensitivity Tests ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Pseudomonas
    Chemical Substances beta-Lactamases (EC 3.5.2.6) ; DNA, Bacterial ; RNA, Ribosomal, 16S ; Agar (9002-18-0) ; Anti-Bacterial Agents
    Language English
    Publishing date 2024-01-07
    Publishing country United States
    Document type Case Reports
    ZDB-ID 604920-5
    ISSN 1879-0070 ; 0732-8893
    ISSN (online) 1879-0070
    ISSN 0732-8893
    DOI 10.1016/j.diagmicrobio.2024.116182
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1845: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Biofilm Formation and Antimicrobial Resistance in Enterococcus.

    Lee, Yangsoon

    Infection & chemotherapy

    2017  Volume 49, Issue 3, Page(s) 236–237

    Language English
    Publishing date 2017-10-13
    Publishing country Korea (South)
    Document type Editorial
    ZDB-ID 2573798-3
    ISSN 2093-2340
    ISSN 2093-2340
    DOI 10.3947/ic.2017.49.3.236
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    Zs.A 6842: Show issues Location:
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  4. Article ; Online: Active Surveillance for Carbapenem-resistant

    Choi, Inho / Lee, Yangsoon

    Annals of laboratory medicine

    2021  Volume 42, Issue 3, Page(s) 367–369

    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Carbapenem-Resistant Enterobacteriaceae ; Carbapenems/pharmacology ; Enterobacteriaceae Infections/diagnosis ; Humans ; Microbial Sensitivity Tests ; Watchful Waiting
    Chemical Substances Anti-Bacterial Agents ; Carbapenems
    Language English
    Publishing date 2021-12-11
    Publishing country Korea (South)
    Document type Letter
    ZDB-ID 2677441-0
    ISSN 2234-3814 ; 2234-3814
    ISSN (online) 2234-3814
    ISSN 2234-3814
    DOI 10.3343/alm.2022.42.3.367
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  5. Article ; Online: Letter to the Editor:

    Kim, Jin-Hong / Lee, Yangsoon

    Annals of clinical and laboratory science

    2021  Volume 51, Issue 4, Page(s) 589–591

    MeSH term(s) Adult ; Dermatitis/diagnosis ; Dermatitis/etiology ; Dermatitis/microbiology ; Female ; Humans ; Immunocompromised Host/physiology ; Oxalobacteraceae/pathogenicity ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA/methods
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2021-08-24
    Publishing country United States
    Document type Case Reports ; Letter
    ZDB-ID 193092-8
    ISSN 1550-8080 ; 0091-7370 ; 0095-8905
    ISSN (online) 1550-8080
    ISSN 0091-7370 ; 0095-8905
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 942: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Genomic Analysis of Carbapenemase-Producing

    Lee, Yangsoon / Kim, Jin-Hong

    Annals of clinical and laboratory science

    2022  Volume 52, Issue 5, Page(s) 811–814

    Abstract: Objective: The increasing trend of : Methods: We randomly selected nine isolates among CPEC isolates carrying KPC, NDM and OXA-type carbapenemase genes for whole genome sequencing (WGS). WGS was performed using the Illumina system (Illumina, Inc., ... ...

    Abstract Objective: The increasing trend of
    Methods: We randomly selected nine isolates among CPEC isolates carrying KPC, NDM and OXA-type carbapenemase genes for whole genome sequencing (WGS). WGS was performed using the Illumina system (Illumina, Inc., San Diego, CA, USA) at Macrogen (Seoul, Korea).
    Results: Seven sequence types were detected: ST131, ST410, ST68, ST216, ST405, ST224 and ST409 variant. Six isolates harbored CTX-M-type genes such as
    Conclusions: The present study showed the diversity of the molecular characteristics of CPEC by analysis of the whole genome of bacteria. WGS is a useful tool that provides much information in the clinical microbiology.
    MeSH term(s) Humans ; Escherichia coli/genetics ; beta-Lactamases/genetics ; Whole Genome Sequencing ; Genomics
    Chemical Substances carbapenemase (EC 3.5.2.6) ; beta-Lactamases (EC 3.5.2.6)
    Language English
    Publishing date 2022-10-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 193092-8
    ISSN 1550-8080 ; 0091-7370 ; 0095-8905
    ISSN (online) 1550-8080
    ISSN 0091-7370 ; 0095-8905
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 942: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: Clinical and microbiological characteristics of female patients with acute pyelonephritis who experienced urinary tract infections within the previous year

    Choseok Yoon / Jeoungyeon Kim / Wooyoung Jang / Ji Won Go / Jinnam Kim / Hyunjoo Pai / Yangsoon Lee / Bongyoung Kim

    Journal of Infection and Public Health, Vol 17, Iss 2, Pp 349-

    2024  Volume 358

    Abstract: Background: This study aimed to examine the clinical and microbiological characteristics of female patients with recurrent acute pyelonephritis (APN). Methods: A retrospective cohort study was conducted at a tertiary care hospital in South Korea from ... ...

    Abstract Background: This study aimed to examine the clinical and microbiological characteristics of female patients with recurrent acute pyelonephritis (APN). Methods: A retrospective cohort study was conducted at a tertiary care hospital in South Korea from July 2019 to December 2021. All female patients aged ≥ 19 years who were diagnosed with community-acquired APN on admission were enrolled. The recurrent group included patients with APN who experienced urinary tract infections within the previous year. The clinical characteristics, types of causative organisms, major antibiotic resistance, and molecular characteristics of Escherichia coli strains were compared between the recurrent and non-recurrent groups. Results: A total of 285 patients with APN were analyzed, including 41 (14.4%) in the recurrent group. Compared to the non-recurrent group, the recurrent group had a higher Charlson Comorbidity Index (1.8 ± 2.1 vs. 1.1 ± 1.5; P = 0.01) and a higher proportion of bladder abnormalities, such as neurogenic bladder (12.2% vs. 2.0%; P = 0.001) and urinary catheterization (12.2% vs. 1.6%; P < 0.001). Escherichia coli was the most common causative organism in both groups. The proportion of Klebsiella pneumoniae (17.1% vs. 4.7%; P = 0.007) and Pseudomonas aeruginosa (5.7% vs. 0.5%; P = 0.014) as a causative organism was higher in the recurrent group. Regarding the microbiological characteristics of Escherichia coli, there were no significant differences in the proportion of antibiotic resistance, phylogenetic groups, resistance genes, and virulence factors between the two groups. Multivariable analysis showed that neurogenic bladder and a history of admission or antibiotic use during 1 year prior to inclusion were significantly associated with recurrent APN. Conclusions: The proportion of causative organisms except Escherichia coli was higher in the recurrent group than in the non-recurrent group. Neurogenic bladder and a history of admission or antibiotic use during 1 year prior to inclusion were risk factors for ...
    Keywords Acute pyelonephritis ; Recurrent ; Urinary tract infection ; South Korea ; Escherichia coli ; Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270
    Subject code 610
    Language English
    Publishing date 2024-02-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Antibiotic Sensitivity and Nasal Microbiome in Patients with Acute Bacterial Rhinosinusitis.

    Gwak, Ho-Jin / Lee, Hyeon A / Jeong, Jae Yeong / Lee, Yangsoon / Rho, Mina / Cho, Seok Hyun

    The Laryngoscope

    2023  Volume 134, Issue 3, Page(s) 1081–1088

    Abstract: Objectives: Acute rhinosinusitis (ARS) is a common upper respiratory tract infection that is mostly of viral origin. However, little is known about the nasal microbiome profile at presentation and the changes caused by antibiotics in acute bacterial ... ...

    Abstract Objectives: Acute rhinosinusitis (ARS) is a common upper respiratory tract infection that is mostly of viral origin. However, little is known about the nasal microbiome profile at presentation and the changes caused by antibiotics in acute bacterial rhinosinusitis (ABRS).
    Methods: This was a prospective single-center study. Overall, 43 ARS patients were screened and were assessed with the symptom questionnaires, nasal endoscopy, and Water's view. Five healthy subjects were recruited as controls. Middle meatal mucus samples were obtained using a cotton swab (for bacterial culture and antimicrobial susceptibility testing) and the suction technique (for 16S rRNA sequencing). After 1 week of antibiotic use (amoxicillin with clavulanic acid), we enrolled 13 patients with ABRS with positive isolates and middle meatal samples for 16S rRNA sequencing were obtained again.
    Results: Overall, we demonstrated a significantly lower abundance of the Lactobacillaceae family in ABRS patients than in healthy controls. Resistant ABRS had different characteristics of middle meatal microbiomes when compared to sensitive ABRS as follows: (1) lower proportion of lactic acid bacteria, (2) increased pathogens such as Rhodococcus sp., Massila sp., Acinetobacter sp., and H. influenza, and (3) increased beta diversity. However, no remarkable changes were observed in the middle meatal microbiome after antibiotic use.
    Conclusion: We showed the roles of Lactobacillaceae in ABRS, and Acinetobacter and Massilia in case of amoxicillin resistance.
    Level of evidence: 3 Laryngoscope, 134:1081-1088, 2024.
    MeSH term(s) Humans ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; RNA, Ribosomal, 16S ; Prospective Studies ; Rhinosinusitis ; Rhinitis/diagnosis ; Sinusitis/diagnosis ; Amoxicillin ; Acute Disease ; Microbiota
    Chemical Substances Anti-Bacterial Agents ; RNA, Ribosomal, 16S ; Amoxicillin (804826J2HU)
    Language English
    Publishing date 2023-08-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80180-x
    ISSN 1531-4995 ; 0023-852X
    ISSN (online) 1531-4995
    ISSN 0023-852X
    DOI 10.1002/lary.30950
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    Ul II Zs.82: Show issues Location:
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  9. Article ; Online: Effects of Immune Cell Heterogeneity and Protein Corona on the Cellular Association and Cytotoxicity of Gold Nanoparticles: A Single-Cell-Based, High-Dimensional Mass Cytometry Study.

    Park, Sehee / Ha, My Kieu / Lee, Yangsoon / Song, Jaewoo / Yoon, Tae Hyun

    ACS nanoscience Au

    2023  Volume 3, Issue 4, Page(s) 323–334

    Abstract: Understanding how nanoparticles (NPs) interact with biological systems is important in many biomedical research areas. However, the heterogeneous nature of biological systems, including the existence of numerous cell types and multitudes of key ... ...

    Abstract Understanding how nanoparticles (NPs) interact with biological systems is important in many biomedical research areas. However, the heterogeneous nature of biological systems, including the existence of numerous cell types and multitudes of key environmental factors, makes these interactions extremely challenging to investigate precisely. Here, using a single-cell-based, high-dimensional mass cytometry approach, we demonstrated that the presence of protein corona has significant influences on the cellular associations and cytotoxicity of gold NPs for human immune cells, and those effects vary significantly with the types of immune cells and their subsets. The altered surface functionality of protein corona reduced the cytotoxicity and cellular association of gold NPs in most cell types (e.g., monocytes, dendritic cells, B cells, natural killer (NK) cells, and T cells) and those immune cells selected different endocytosis pathways such as receptor-mediated endocytosis, phagocytosis, and micropinocytosis. However, even slight alterations in the major cell type (phagocytic cells and non-phagocytic cells) and T cell subsets (e.g., memory and naive T cells) resulted in significant protein corona-dependent variations in their cellular dose of gold NPs. Especially, naive T killer cells exhibited additional heterogeneity than memory T killer cells, with clusters exhibiting distinct cellular association patterns in single-cell contour plots. This multi-parametric analysis of mass cytometry data established a conceptual framework for a more holistic understanding of how the human immune system responds to external stimuli, paving the way for the application of precisely engineered NPs as promising tools of nanomedicine under various clinical settings, including targeted drug delivery and vaccine development.
    Language English
    Publishing date 2023-04-24
    Publishing country United States
    Document type Journal Article
    ISSN 2694-2496
    ISSN (online) 2694-2496
    DOI 10.1021/acsnanoscienceau.3c00001
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  10. Article ; Online: Virulence Factors Associated With

    Kim, Bongyoung / Kim, Jin-Hong / Lee, Yangsoon

    Annals of laboratory medicine

    2021  Volume 42, Issue 2, Page(s) 203–212

    Abstract: Background: Extraintestinal pathogenic : Methods: We conducted a single-center, prospective study in an 855-bed tertiary-care hospital in Korea. We consecutively recruited 80 hospitalized UTI patients with : Results: We found no significant ... ...

    Abstract Background: Extraintestinal pathogenic
    Methods: We conducted a single-center, prospective study in an 855-bed tertiary-care hospital in Korea. We consecutively recruited 80 hospitalized UTI patients with
    Results: We found no significant differences in STs, antimicrobial resistance patterns, or VFs between isolates from blood and urine specimens. ST131, a pandemic multidrug-resistant clone present in both blood and urine, was the most frequent ST (N=19/80, 24%), and ST131 isolates carried more virulence genes, especially,
    Conclusions: We found no STs and VFs associated with bacteremia in WGS data of
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Bacteremia/diagnosis ; Escherichia coli/genetics ; Escherichia coli Infections/diagnosis ; Humans ; Prospective Studies ; Urinary Tract Infections/diagnosis ; Virulence Factors/genetics
    Chemical Substances Anti-Bacterial Agents ; Virulence Factors
    Language English
    Publishing date 2021-10-08
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 2677441-0
    ISSN 2234-3814 ; 2234-3814
    ISSN (online) 2234-3814
    ISSN 2234-3814
    DOI 10.3343/alm.2022.42.2.203
    Database MEDical Literature Analysis and Retrieval System OnLINE

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