LIVIVO - Search results -

Search results

Result 1 - 10 of total 607

Search options

Article ; Online: PIF-pocket as a target for C. albicans Pkh selective inhibitors.

Pastor-Flores, Daniel / Schulze, Jörg O / Bahí, Anna / Giacometti, Romina / Ferrer-Dalmau, Jofre / Passeron, Susana / Engel, Matthias / Süss, Evelyn / Casamayor, Antonio / Biondi, Ricardo M

ACS chemical biology

2013 Volume 8, Issue 10, Page(s) 2283–2292

Abstract: ... not inhibit the human ortholog. Here, we describe C. albicans Pkh2 biochemically, structurally and ... by using chemical probes in comparison to human PDK1. We found that a regulatory site on the C. albicans ... for C. albicans Pkh2. Together, our results describe novel features of the biology of Pkh and chemical ...

Abstract	The phosphoinositide-dependent protein kinase 1, PDK1, is a master kinase that phosphorylates the activation loop of up to 23 AGC kinases. S. cerevisiae has three PDK1 orthologues, Pkh1-3, which also phosphorylate AGC kinases (e.g., Ypk, Tpk, Pkc1, and Sch9). Pkh1 and 2 are redundant proteins involved in multiple essential cellular functions, including endocytosis and cell wall integrity. Based on similarities with the budding yeast, the Pkh of fungal infectious species was postulated as a novel target for antifungals. Here, we found that depletion of Pkh eventually induces oxidative stress and DNA double-strand breaks, leading to programmed cell death. This finding supports Pkh as an antifungal target since pharmacological inhibition of Pkh would lead to the death of yeast cells, the ultimate goal of antifungals. It was therefore of interest to further investigate the possibility to develop Pkh inhibitors with selectivity for Candida Pkh that would not inhibit the human ortholog. Here, we describe C. albicans Pkh2 biochemically, structurally and by using chemical probes in comparison to human PDK1. We found that a regulatory site on the C. albicans Pkh2 catalytic domain, the PIF-pocket, diverges from human PDK1. Indeed, we identified and characterized PS77, a new small allosteric inhibitor directed to the PIF-pocket, which has increased selectivity for C. albicans Pkh2. Together, our results describe novel features of the biology of Pkh and chemical biology approaches that support the validation of Pkh as a drug target for selective antifungals.
MeSH term(s)	Allosteric Regulation ; Antifungal Agents/chemistry ; Antifungal Agents/pharmacology ; Binding Sites ; Candida albicans/drug effects ; Candida albicans/enzymology ; Chalcones/chemistry ; Chalcones/pharmacology ; Drug Delivery Systems ; Enzyme Activation/drug effects ; Humans ; Models, Molecular ; Protein Kinase Inhibitors/chemistry ; Protein Kinase Inhibitors/pharmacology ; Protein Kinases/chemistry ; Protein Kinases/metabolism ; Reactive Oxygen Species ; Receptors, Neurotransmitter/metabolism ; Thioglycolates/chemistry ; Thioglycolates/pharmacology
Chemical Substances	2-(3-(4-chlorophenyl)-1-(2-(4-chlorophenylthio)phenyl)-3-oxopropylthio)acetic acid ; Antifungal Agents ; Chalcones ; Protein Kinase Inhibitors ; Reactive Oxygen Species ; Receptors, Neurotransmitter ; Thioglycolates ; prolactin-inhibitory factor receptor ; Protein Kinases (EC 2.7.-)
Language	English
Publishing date	2013-10-18
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1554-8937
ISSN (online)	1554-8937
DOI	10.1021/cb400452z
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Editorial on the Special Issue Titled "Pathology and Diagnosis of Gynecologic Diseases".

Giacometti, Cinzia / Ludwig, Kathrin

Diagnostics (Basel, Switzerland)

2023 Volume 13, Issue 22

Abstract: In the medical and diagnostic daily routine, gynecologic diseases present many different scenarios [ ... ]. ...

Abstract	In the medical and diagnostic daily routine, gynecologic diseases present many different scenarios [...].
Language	English
Publishing date	2023-11-20
Publishing country	Switzerland
Document type	Editorial
ZDB-ID	2662336-5
ISSN	2075-4418
ISSN	2075-4418
DOI	10.3390/diagnostics13223480
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Multiple routes of communication within the amygdala-mPFC network: A comparative approach in humans and macaques.

Giacometti, C / Amiez, C / Hadj-Bouziane, F

Current research in neurobiology

2023 Volume 5, Page(s) 100103

Abstract: The network formed by the amygdala (AMG) and the medial Prefrontal Cortex (mPFC), at the interface between our internal and external environment, has been shown to support some important aspects of behavioral adaptation. Whether and how the anatomo- ... ...

Abstract	The network formed by the amygdala (AMG) and the medial Prefrontal Cortex (mPFC), at the interface between our internal and external environment, has been shown to support some important aspects of behavioral adaptation. Whether and how the anatomo-functional organization of this network evolved across primates remains unclear. Here, we compared AMG nuclei morphological characteristics and their functional connectivity with the mPFC in humans and macaques to identify potential homologies and differences between these species. Based on selected studies, we highlight two subsystems within the AMG-mPFC circuits, likely involved in distinct temporal dynamics of integration during behavioral adaptation. We also show that whereas the mPFC displays a large expansion but a preserved intrinsic anatomo-functional organization, the AMG displays a volume reduction and morphological changes related to specific nuclei. We discuss potential commonalities and differences in the dialogue between AMG nuclei and mPFC in humans and macaques based on available data.
Language	English
Publishing date	2023-07-28
Publishing country	Netherlands
Document type	Journal Article ; Review
ISSN	2665-945X
ISSN (online)	2665-945X
DOI	10.1016/j.crneur.2023.100103
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Gastro-Esophageal Junction Precancerosis: Histological Diagnostic Approach and Pathogenetic Insights.

Giacometti, Cinzia / Gusella, Anna / Cassaro, Mauro

Cancers

2023 Volume 15, Issue 24

Abstract: Barrett's esophagus (BE) was initially defined in the 1950s as the visualization of gastric-like mucosa in the esophagus. Over time, the definition has evolved to include the identification of goblet cells, which confirm the presence of intestinal ... ...

Abstract	Barrett's esophagus (BE) was initially defined in the 1950s as the visualization of gastric-like mucosa in the esophagus. Over time, the definition has evolved to include the identification of goblet cells, which confirm the presence of intestinal metaplasia within the esophagus. Chronic gastro-esophageal reflux disease (GERD) is a significant risk factor for adenocarcinoma of the esophagus, as intestinal metaplasia can develop due to GERD. The development of adenocarcinomas related to BE progresses in sequence from inflammation to metaplasia, dysplasia, and ultimately carcinoma. In the presence of GERD, the squamous epithelium changes to columnar epithelium, which initially lacks goblet cells, but later develops goblet cell metaplasia and eventually dysplasia. The accumulation of multiple genetic and epigenetic alterations leads to the development and progression of dysplasia. The diagnosis of BE requires the identification of intestinal metaplasia on histologic examination, which has thus become an essential tool both in the diagnosis and in the assessment of dysplasia's presence and degree. The histologic diagnosis of BE dysplasia can be challenging due to sampling error, pathologists' experience, interobserver variation, and difficulty in histologic interpretation: all these problems complicate patient management. The development and progression of Barrett's esophagus (BE) depend on various molecular events that involve changes in cell-cycle regulatory genes, apoptosis, cell signaling, and adhesion pathways. In advanced stages, there are widespread genomic abnormalities with losses and gains in chromosome function, and DNA instability. This review aims to provide an updated and comprehensible diagnostic approach to BE based on the most recent guidelines available in the literature, and an overview of the pathogenetic and molecular mechanisms of its development.
Language	English
Publishing date	2023-12-06
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers15245725
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Response to raltegravir-based salvage therapy in HIV-infected patients with hepatitis C virus or hepatitis B virus coinfection.

Weimer, Liliana Elena / Fragola, Vincenzo / Floridia, Marco / Guaraldi, Giovanni / Ladisa, Nicoletta / Francisci, Daniela / Bellagamba, Rita / Degli Antoni, Anna / Parruti, Giustino / Giacometti, Andrea / Manconi, Paolo Emilio / Vivarelli, Angela / D'Ettorre, Gabriella / Mura, Maria Stella / Cicalini, Stefania / Preziosi, Roberta / Sighinolfi, Laura / Verucchi, Gabriella / Libertone, Raffaella /

Tavio, Marcello / Sarmati, Loredana / Bucciardini, Raffaella

The Journal of antimicrobial chemotherapy

2012 Volume 68, Issue 1, Page(s) 193–199

Abstract: Objectives: To define the impact of coinfection with hepatitis B virus (HBV) or hepatitis C ...

Abstract	Objectives: To define the impact of coinfection with hepatitis B virus (HBV) or hepatitis C virus (HCV) on viroimmunological response to raltegravir-based salvage regimens that also include new HIV inhibitors such as maraviroc, darunavir and etravirine. Methods: We used data from a national observational study of patients starting raltegravir-based regimens to compare virological suppression and CD4 cell change from baseline in patients with and without concomitant HBV or HCV infection. Results: Overall, 275 patients (107 coinfected and 168 non-coinfected) were evaluated. Coinfected patients were more commonly former intravenous drug users and had a longer history of HIV infection and higher baseline aminotransferase levels. Both HIV-RNA and CD4 response were similar in the two groups. Mean time to first HIV-RNA copy number <50 copies/mL was 4.1 months (95% CI 3.5-4.6) in non-coinfected patients and 3.9 months (95% CI 3.3-4.5) in coinfected patients (hazard ratio 1.039, 95% CI 0.761-1.418, P = 0.766, log-rank test). The risk of developing new grade 3-4 hepatic adverse events was significantly higher in coinfected patients (hazard ratio 1.779, 95% CI 1.123-2.817, P = 0.009). The two groups of coinfected and non-coinfected patients had similar rates of interruption of any baseline drug (hazard ratio 1.075, 95% CI 0.649-1.781, P = 0.776) and of raltegravir (hazard ratio 1.520, 95% CI 0.671-3.447, P = 0.311). Few AIDS-defining events and deaths occurred. Conclusions: Viroimmunological response to regimens based on raltegravir and other recent anti-HIV inhibitors is not negatively affected by coinfection with HBV or HCV. Liver toxicity, either pre-existing or new, is more common in coinfected patients, but with no increased risk of treatment interruption.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Cohort Studies ; Coinfection/drug therapy ; Coinfection/epidemiology ; Female ; Follow-Up Studies ; HIV Infections/drug therapy ; HIV Infections/epidemiology ; Hepacivirus/drug effects ; Hepatitis B/drug therapy ; Hepatitis B/epidemiology ; Hepatitis B virus/drug effects ; Hepatitis C/drug therapy ; Hepatitis C/epidemiology ; Humans ; Male ; Middle Aged ; Salvage Therapy/methods ; Treatment Outcome ; Young Adult
Language	English
Publishing date	2012-09-14
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	191709-2
ISSN	1460-2091 ; 0305-7453
ISSN (online)	1460-2091
ISSN	0305-7453
DOI	10.1093/jac/dks341
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1197: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 124: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: The association of high-sensitivity c-reactive protein and other biomarkers with cardiovascular disease in patients treated for HIV: a nested case-control study.

De Luca, Andrea / de Gaetano Donati, Katleen / Colafigli, Manuela / Cozzi-Lepri, Alessandro / De Curtis, Amalia / Gori, Andrea / Sighinolfi, Laura / Giacometti, Andrea / Capobianchi, Maria Rosaria / D'Avino, Alessandro / Iacoviello, Licia / Cauda, Roberto / D'Arminio Monforte, Antonella

BMC infectious diseases

2013 Volume 13, Page(s) 414

Abstract: Background: Elevated high-sensitivity C-reactive protein (hsCRP) increases the risk ...

Abstract	Background: Elevated high-sensitivity C-reactive protein (hsCRP) increases the risk of cardiovascular disease (CVD) in the general population, but its role as a predictive marker in HIV-positive patients remains unclear. Aim of the study was to evaluate whether hsCRP or other biomarkers are independent predictors of CVD risk in HIV-infected patients. Methods: Retrospective, nested case-control study. HIV-positive men and women (35-69 years of age) receiving combination antiretroviral therapy (cART) were included. Cases (n = 35) had a major CVD event. Controls (n = 74) free from CVD events for at least 5 years from starting ART were matched on diabetes and smoking. HsCRP, D-dimer, P-selectin, interleukin-6 (IL-6), tissue plasminogen activator, plasminogen activator inhibitor-1 levels were measured. Results: High hsCRP was associated with CVD risk, independently of traditional cardiovascular risk factors, HIV replication and the type of ART received at the time of sampling (adjusted odds ratio 8.00 [1.23-51.94] comparing >3.3 mg/L with <0.9 mg/L; P = 0.03). Higher IL-6 and P-selectin levels were also independently associated with increased CVD risk, although the association was weaker than for hsCRP. Higher total cholesterol and lower HDL cholesterol increased CVD risk, independent of hsCRP. Conclusion: hsCRP may be a useful additional biomarker to predict CVD risk in HIV-infected patients receiving cART.
MeSH term(s)	Adult ; Aged ; Anti-HIV Agents/therapeutic use ; Biomarkers/blood ; C-Reactive Protein/analysis ; Cardiovascular Diseases/blood ; Cardiovascular Diseases/diagnosis ; Cardiovascular Diseases/etiology ; Case-Control Studies ; Female ; HIV Infections/blood ; HIV Infections/complications ; HIV Infections/drug therapy ; Humans ; Interleukin-6/blood ; Male ; Middle Aged ; Retrospective Studies ; Risk Factors
Chemical Substances	Anti-HIV Agents ; Biomarkers ; Interleukin-6 ; C-Reactive Protein (9007-41-4)
Language	English
Publishing date	2013-09-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1471-2334
ISSN (online)	1471-2334
DOI	10.1186/1471-2334-13-414
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: How seasonality influences the thermal biology of lizards with different thermoregulatory strategies: a meta-analysis.

Giacometti, Danilo / Palaoro, Alexandre V / Leal, Laura C / de Barros, Fábio C

Biological reviews of the Cambridge Philosophical Society

2023 Volume 99, Issue 2, Page(s) 409–429

Abstract: Ectotherms that maintain thermal balance in the face of varying climates should be able to colonise a wide range of habitats. In lizards, thermoregulation usually appears as a variety of behaviours that buffer external influences over physiology. Basking ...

Abstract	Ectotherms that maintain thermal balance in the face of varying climates should be able to colonise a wide range of habitats. In lizards, thermoregulation usually appears as a variety of behaviours that buffer external influences over physiology. Basking species rely on solar radiation to raise body temperatures and usually show high thermoregulatory precision. By contrast, species that do not bask are often constrained by climatic conditions in their habitats, thus having lower thermoregulatory precision. While much focus has been given to the effects of mean habitat temperatures, relatively less is known about how seasonality affects the thermal biology of lizards on a macroecological scale. Considering the current climate crisis, assessing how lizards cope with temporal variations in environmental temperature is essential to understand better how these organisms will fare under climate change. Activity body temperatures (T
MeSH term(s)	Humans ; Animals ; Lizards/physiology ; Phylogeny ; Body Temperature Regulation ; Temperature ; Biology ; Body Temperature
Language	English
Publishing date	2023-10-23
Publishing country	England
Document type	Meta-Analysis ; Journal Article
ZDB-ID	1423558-4
ISSN	1469-185X ; 0006-3231 ; 1464-7931
ISSN (online)	1469-185X
ISSN	0006-3231 ; 1464-7931
DOI	10.1111/brv.13028
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 71/734: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Performances of two rapid LAMP-based techniques for the intrapartum detection of Group B Streptococcus vaginal colonization.

Charfi, Rym / Guyonnet, Cécile / Untrau, Meiggie / Giacometti, Gaëlle / Paper, Thierry / Poyart, Claire / Plainvert, Céline / Tazi, Asmaa

Annals of clinical microbiology and antimicrobials

2024 Volume 23, Issue 1, Page(s) 37

Abstract: Purpose: Group B Streptococcus (GBS) is the leading cause of invasive infections in newborns. The prevention of GBS neonatal disease relies on the administration of an intrapartum antibiotic prophylaxis to GBS-colonized women. In recent years, rapid ... ...

Abstract	Purpose: Group B Streptococcus (GBS) is the leading cause of invasive infections in newborns. The prevention of GBS neonatal disease relies on the administration of an intrapartum antibiotic prophylaxis to GBS-colonized women. In recent years, rapid intrapartum detection of GBS vaginal colonization using real-time nucleic acid amplification tests (NAATs) emerged as an alternative to antenatal culture screening methods. Methods: We compared the performances of two loop-mediated isothermal amplification (LAMP) tests, the Ampliflash® GBS and the PlusLife® GBS tests, to standard culture for GBS detection in vaginal specimens from pregnant women. The study was conducted from April to July 2023 in a French hospital of the Paris area. Results: A total of 303 samples were analyzed, including 85 culture-positive samples (28.1%). The Ampliflash® GBS test and the PlusLife® GBS tests gave a result for 100% and 96.3% tests, respectively. The performances of the tests were as follows: sensitivity 87.1% (95% confidence interval (CI) 78.3-92.6) and 98.7% (95% CI 93.0-99.8), specificity 99.1% (95% CI 96.7-99.8), and 91.9% (95% CI 87.3-95.0), respectively. False negative results of the Ampliflash® GBS test correlated with low-density GBS cultures. Time-to-results correlated with GBS culture density only for the PlusLife® GBS test (p < 0.001). Conclusion: Both techniques provide excellent analytical performances with high sensitivity and specificity together with a short turnaround time and results available in 10 to 35 min. Their potential to further reduce the burden of GBS neonatal disease compared with antenatal culture screening needs to be assessed in future clinical studies.
MeSH term(s)	Humans ; Female ; Nucleic Acid Amplification Techniques/methods ; Streptococcus agalactiae/genetics ; Streptococcus agalactiae/isolation & purification ; Pregnancy ; Streptococcal Infections/diagnosis ; Streptococcal Infections/microbiology ; Vagina/microbiology ; Pregnancy Complications, Infectious/diagnosis ; Pregnancy Complications, Infectious/microbiology ; Sensitivity and Specificity ; Molecular Diagnostic Techniques/methods ; Infant, Newborn ; Adult
Language	English
Publishing date	2024-04-25
Publishing country	England
Document type	Journal Article ; Comparative Study
ZDB-ID	2097873-X
ISSN	1476-0711 ; 1476-0711
ISSN (online)	1476-0711
ISSN	1476-0711
DOI	10.1186/s12941-024-00695-2
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Characterisation of Monovarietal Olive Oils Obtained from Croatian

Giacometti, Jasminka / Milin, Čedomila / Giacometti, Fabio / Ciganj, Zlatko

Foods (Basel, Switzerland)

2018 Volume 7, Issue 11

Abstract: The aim of this study was the monitoring of the chemical composition of olive oil at different ripening stages to determine the appropriate harvesting time during any given crop season in the northern Adriatic region. For this purpose, from September to ... ...

Abstract	The aim of this study was the monitoring of the chemical composition of olive oil at different ripening stages to determine the appropriate harvesting time during any given crop season in the northern Adriatic region. For this purpose, from September to November, two Croatian olive cultivars (Drobnica and Buza) were taken from two different olive orchards and for the respective olive oils, prepared on a laboratory scale, the major saponifiable, unsaponifiable and phenolic compounds were determined. Based on the chemical analyses performed, the optimal harvesting time has been set in October for both cultivars. Buza had a higher oleic acid, but lower total sterols, squalene and total alkanols. Compared to the local cultivars, the studied cultivars had a high total phenolic content and antioxidant activity. The antioxidant activity and concentrations of total phenols correlated with α-tocopherol in oil samples taken during the ripening progress. Finally, trace minerals detected in Buza and Drobnica oil differed, which can be an indicator of oxidative stability and authenticity of oils.
Language	English
Publishing date	2018-11-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods7110188
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Kaposi Sarcoma in People Living with HIV: Is it Water under the Bridge?

Papalini, Chiara / Brescini, Lucia / Curci, Laura / Bastianelli, Sabrina / Barchiesi, Francesco / Giacometti, Andrea / Francisci, Daniela

Mediterranean journal of hematology and infectious diseases

2023 Volume 15, Issue 1, Page(s) e2023027

Language	English
Publishing date	2023-05-01
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2674750-9
ISSN	2035-3006
ISSN	2035-3006
DOI	10.4084/MJHID.2023.027
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito