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  1. Article: High-throughput analysis of DNA repair in microplates towards identification of inhibitors.

    Kamiya, Hiroyuki

    Genes and environment : the official journal of the Japanese Environmental Mutagen Society

    2020  Volume 42, Page(s) 11

    Abstract: Environmental factors can inhibit DNA repair and cause indirect mutagenic actions. Here the author introduces a recent paper on a novel high-throughput system to analyze the various enzymatic activities involved in base excision repair. Such systems will ...

    Abstract Environmental factors can inhibit DNA repair and cause indirect mutagenic actions. Here the author introduces a recent paper on a novel high-throughput system to analyze the various enzymatic activities involved in base excision repair. Such systems will facilitate the identification of compounds that suppressively affect DNA repair.
    Language English
    Publishing date 2020-03-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 2269162-5
    ISSN 1880-7062 ; 1880-7046
    ISSN (online) 1880-7062
    ISSN 1880-7046
    DOI 10.1186/s41021-020-00153-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: [Low Output Syndrome:Points to Consider for Intraoperative Myocardial Protection and Treatment Methods].

    Suzuki, Fumitaka / Kamiya, Hiroyuki

    Kyobu geka. The Japanese journal of thoracic surgery

    2023  Volume 76, Issue 10, Page(s) 751–755

    Abstract: Low cardiac output syndrome (LOS) is a condition that causes low perfusion and perfusion failure of the entire body's tissues due to a decline in heart contractile strength, posing a significant challenge in cardiothoracic surgical perioperative ... ...

    Abstract Low cardiac output syndrome (LOS) is a condition that causes low perfusion and perfusion failure of the entire body's tissues due to a decline in heart contractile strength, posing a significant challenge in cardiothoracic surgical perioperative management. Appropriate myocardial protection is crucial to prevent ischemia-reperfusion injury during open-heart surgery and prevent LOS. The integrated myocardial protection method, proposed by Buckberg et al., is one technique employed for this purpose. In the treatment of LOS, interventions are made in the parameters of stroke volume and heart rate, structural abnormalities are excluded and dealt with, and mechanical assistance is utilized when necessary. With the aging and increasing severity of surgical patients, the risk of postoperative LOS is on the rise. Therefore, the application of appropriate myocardial protection and treatment methods leads to improved prognosis. It's worth noting that ensuring optimal myocardial protection during surgery and the correct application of medication and devices for intervention can significantly improve patient outcomes. With the rise in high-risk surgical cases due to aging and an increase in severe conditions, the importance of these interventions cannot be overstated.
    MeSH term(s) Humans ; Cardiac Output, Low ; Cardiac Surgical Procedures/adverse effects ; Reperfusion Injury/prevention & control ; Perioperative Period
    Language Japanese
    Publishing date 2023-12-13
    Publishing country Japan
    Document type English Abstract ; Journal Article
    ZDB-ID 603899-2
    ISSN 0021-5252
    ISSN 0021-5252
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 11: Show issues Location:
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  3. Article ; Online: High-throughput analysis of DNA repair in microplates towards identification of inhibitors

    Hiroyuki Kamiya

    Genes and Environment, Vol 42, Iss 1, Pp 1-

    2020  Volume 3

    Abstract: Abstract Environmental factors can inhibit DNA repair and cause indirect mutagenic actions. Here the author introduces a recent paper on a novel high-throughput system to analyze the various enzymatic activities involved in base excision repair. Such ... ...

    Abstract Abstract Environmental factors can inhibit DNA repair and cause indirect mutagenic actions. Here the author introduces a recent paper on a novel high-throughput system to analyze the various enzymatic activities involved in base excision repair. Such systems will facilitate the identification of compounds that suppressively affect DNA repair.
    Keywords DNA repair ; Base excision repair ; High-throughput analysis ; Ecology ; QH540-549.5 ; Genetics ; QH426-470
    Language English
    Publishing date 2020-03-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Correction of substitution, deletion, and insertion mutations by 5'-tailed duplexes.

    Kawai, Hidehiko / Sato, Kento / Kato, Taiki / Kamiya, Hiroyuki

    Journal of bioscience and bioengineering

    2024  Volume 137, Issue 3, Page(s) 157–164

    Abstract: Germline and somatic mutations cause various diseases, including cancer. Clinical applications of genome editing are keenly anticipated, since it can cure genetic diseases. Recently, we reported that a 5'-tailed duplex (TD), consisting of an ... ...

    Abstract Germline and somatic mutations cause various diseases, including cancer. Clinical applications of genome editing are keenly anticipated, since it can cure genetic diseases. Recently, we reported that a 5'-tailed duplex (TD), consisting of an approximately 80-base editor strand oligodeoxyribonucleotide and a 35-base assistant strand oligodeoxyribonucleotide, could edit a target gene on plasmid DNA and correct a single-base substitution mutation without an artificial nuclease in human cells. In this study, we assessed the ability of the TD to correct base substitution mutations located consecutively or separately, and deletion and insertion mutations. A TD with an 80-base editor strand was co-introduced into human U2OS cells with plasmid DNA bearing either a wild-type or mutated copepod green fluorescent protein (copGFP) gene. Among the mutations, three-base consecutive substitutions were efficiently repaired. The correction efficiencies of deletion mutations were similar to those of substitution mutations, and two to three times higher than those of insertion mutations. Up to three-base substitution, deletion, and insertion mutations were excellent targets for correction by TDs. These results suggested that the TDs are useful for editing disease-causing genes with small mutations.
    Language English
    Publishing date 2024-01-11
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 1465387-4
    ISSN 1347-4421 ; 1389-1723
    ISSN (online) 1347-4421
    ISSN 1389-1723
    DOI 10.1016/j.jbiosc.2023.12.011
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 4728: Show issues

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  5. Article ; Online: A bailout procedure with a surgical stapler for unsuccessful left atrium appendage clipping in minimally invasive cardiac surgery.

    Shirasaka, Tomonori / Shirakura, Kentaro / Setogawa, Yuki / Kamiya, Hiroyuki

    JTCVS techniques

    2023  Volume 18, Page(s) 57–59

    Language English
    Publishing date 2023-01-10
    Publishing country United States
    Document type Case Reports
    ISSN 2666-2507
    ISSN (online) 2666-2507
    DOI 10.1016/j.xjtc.2022.11.018
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Unexpectedly stable homopurine parallel triplex of SNA:RNA*SNA and L-

    Kamiya, Yukiko / Lao, Siyuan / Ariyoshi, Jumpei / Sato, Fuminori / Asanuma, Hiroyuki

    Chemical communications (Cambridge, England)

    2024  Volume 60, Issue 10, Page(s) 1257–1260

    Abstract: Homopurine strands are known to form antiparallel triplexes stabilized by G*G and A*A Hoogsteen pairs, which have two hydrogen bonds. But there has been no report on the parallel triplex formation of homopurine involving both adenosine and guanosine to ... ...

    Abstract Homopurine strands are known to form antiparallel triplexes stabilized by G*G and A*A Hoogsteen pairs, which have two hydrogen bonds. But there has been no report on the parallel triplex formation of homopurine involving both adenosine and guanosine to the duplex. In this paper, we first report parallel triplex formation between a homopurine serinol nucleic acid (SNA) strand and an RNA/SNA duplex. Melting profiles revealed that the parallel SNA:RNA*SNA triplex was remarkably stable, even though the A*A pair has a single hydrogen bond. An L-acyclic threoninol nucleic acid (L-
    MeSH term(s) Nucleic Acids/chemistry ; RNA/chemistry ; Amino Alcohols/chemistry ; Nucleic Acid Conformation ; Butylene Glycols ; Propylene Glycols ; Propanolamines
    Chemical Substances Nucleic Acids ; RNA (63231-63-0) ; threoninol ; serinol (IC94L30J8M) ; Amino Alcohols ; Butylene Glycols ; Propylene Glycols ; Propanolamines
    Language English
    Publishing date 2024-01-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/d3cc05555h
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: A Rare Case of Leukemoid Reaction During Mechanical Circulatory Support in a Patient With Severe Heart Failure: An Autopsy Study.

    Kunioka, Shingo / Suzuki, Fumitaka / Nagata, Marino / Tsutsui, Masahiro / Kamiya, Hiroyuki

    Cureus

    2024  Volume 16, Issue 2, Page(s) e54603

    Abstract: The leukemoid reaction (LR) is reported to be caused by severe stress conditions such as infection, malignancies, intoxication, severe hemorrhage, or acute hemolysis; this condition is attributed to a very severe prognosis. Some reports have suggested ... ...

    Abstract The leukemoid reaction (LR) is reported to be caused by severe stress conditions such as infection, malignancies, intoxication, severe hemorrhage, or acute hemolysis; this condition is attributed to a very severe prognosis. Some reports have suggested that the LR was associated with a systemic stress response. A 36-year-old man who required mechanical circulatory support (MCS), including veno-arterial extracorporeal membrane oxygenation and Impella 5.5 due to severe heart failure, was transferred to our hospital. He showed a markedly elevated WBC count and died of multiple organ failure. The autopsy revealed the possibility that leukocytosis might have been due to an LR; however, the cause of the cardiac failure was unknown. To the best of our knowledge, this study is the first to report a rare case of LR in a patient with severe heart failure requiring MCS.
    Language English
    Publishing date 2024-02-21
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2747273-5
    ISSN 2168-8184
    ISSN 2168-8184
    DOI 10.7759/cureus.54603
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: A Rapidly Progressive Aortic Aneurysm Due to Escherichia Coli.

    Nagahata, Ken / Kamiya, Hiroyuki / Takahashi, Hiroki

    The American journal of medicine

    2023  Volume 136, Issue 6, Page(s) e109–e110

    MeSH term(s) Humans ; Escherichia coli ; Aortic Aneurysm ; Aortic Aneurysm, Abdominal/diagnosis ; Aortic Aneurysm, Abdominal/diagnostic imaging ; Escherichia coli Infections/complications ; Escherichia coli Infections/diagnosis ; Escherichia coli Infections/drug therapy
    Language English
    Publishing date 2023-02-22
    Publishing country United States
    Document type Case Reports
    ZDB-ID 80015-6
    ISSN 1555-7162 ; 1873-2178 ; 0002-9343 ; 1548-2766
    ISSN (online) 1555-7162 ; 1873-2178
    ISSN 0002-9343 ; 1548-2766
    DOI 10.1016/j.amjmed.2023.01.041
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ua VI Zs.289: Show issues Location:
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  9. Article: [Minimally Invasive Cardiac Surgery with Thoracoscopy].

    Kamiya, Hiroyuki

    Kyobu geka. The Japanese journal of thoracic surgery

    2018  Volume 71, Issue 10, Page(s) 737–741

    Abstract: Minimally invasive cardiac surgery (MICS) with high quality thoracoscopy has been widespread. MICS has been very well established for mitral valve repair because of its anatomical condition. For aortic valve surgery and coronary artery surgery, MICS has ... ...

    Abstract Minimally invasive cardiac surgery (MICS) with high quality thoracoscopy has been widespread. MICS has been very well established for mitral valve repair because of its anatomical condition. For aortic valve surgery and coronary artery surgery, MICS has not been so widespread in comparison to mitral valve surgery, but there has yet room for further development. Here we review current status of thoracoscopy for MICS.
    MeSH term(s) Aortic Valve/surgery ; Cardiac Surgical Procedures/methods ; Humans ; Minimally Invasive Surgical Procedures/methods ; Mitral Valve/surgery ; Thoracoscopy
    Language Japanese
    Publishing date 2018-10-11
    Publishing country Japan
    Document type Journal Article ; Review
    ZDB-ID 603899-2
    ISSN 0021-5252
    ISSN 0021-5252
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 11: Show issues Location:
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  10. Article: Easily-controllable, helper phage-free single-stranded phagemid production system.

    Suzuki, Tetsuya / Kamiya, Hiroyuki

    Genes and environment : the official journal of the Japanese Environmental Mutagen Society

    2022  Volume 44, Issue 1, Page(s) 25

    Abstract: Background: Single-stranded (ss) DNAs are utilized in various molecular biological and biotechnological applications including the construction of double-stranded DNAs with a DNA lesion, and are commonly prepared by using chimeric phage-plasmids ( ... ...

    Abstract Background: Single-stranded (ss) DNAs are utilized in various molecular biological and biotechnological applications including the construction of double-stranded DNAs with a DNA lesion, and are commonly prepared by using chimeric phage-plasmids (phagemids) plus M13-derived helper phages. However, the yields of ss DNA with these methods are poorly reproducible, and multiple factors must be optimized.
    Results: In this report, we describe a new arabinose-inducible ss phagemid production method without helper phage infection. The newly exploited DNA derived from VCSM13 expresses the pII protein, which initiates ss DNA synthesis, under the control of the araBAD promoter. In addition, the packaging signal is deleted in the DNA to reduce the contamination of the phage-derived ss DNA. The phagemid DNA of interest, carrying the M13 origin of replication and the packaging signal, was introduced into bacterial cells maintaining the modified VCSM13 DNA as a plasmid, and the ss phagemid DNA production was induced by arabinose. The DNA recovered from the phage particles had less contamination from VCSM13 DNA, as compared to the conventional method. Moreover, we extended the method to purify the ss DNAs by using an anion-exchange column, to avoid the use of hazardous chemicals.
    Conclusion: Using this combination of methods, large quantities of phagemid ss DNAs of interest can be consistently obtained.
    Language English
    Publishing date 2022-11-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 2269162-5
    ISSN 1880-7062 ; 1880-7046
    ISSN (online) 1880-7062
    ISSN 1880-7046
    DOI 10.1186/s41021-022-00254-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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