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  1. Article ; Online: Mutational analysis of catalytic site domain of CCHFV L RNA segment.

    Kaushal, Neha / Baranwal, Manoj

    Journal of molecular modeling

    2023  Volume 29, Issue 4, Page(s) 88

    Abstract: Introduction: Crimean-Congo haemorrhagic fever virus (CCHFV) has tripartite RNA genome and is endemic in various countries of Asia, Africa and Europe.: Method: The present study is focused on mutation profiling of CCHFV L segment and phylogenetic ... ...

    Abstract Introduction: Crimean-Congo haemorrhagic fever virus (CCHFV) has tripartite RNA genome and is endemic in various countries of Asia, Africa and Europe.
    Method: The present study is focused on mutation profiling of CCHFV L segment and phylogenetic clustering of protein dataset into six CCHFV genotypes.
    Results: Phylogenetic tree rooted with NCBI reference sequence (YP_325663.1) indicated less divergence from genotype III and the sequences belonging to same genotypes have shown less divergence among each other. Mutation frequency at 729 mutated positions was calculated and 563, 49, 33, 46 and 38 amino acid positions were found to be mutated at mutation frequency intervals of 0-0.2, 0.21-0.4, 0.41-0.6, 0.61-0.8 and 0.81-1.0 respectively. Thirty-eight highly frequent mutations (0.81-1.0 interval) were found in all genotypes and mapping in L segment (encoded for RdRp) revealed four mutations (V2074I, I2134T/A, V2148A and Q2695H/R) in catalytic site domain and no mutation in OTU domain. Molecular dynamic simulation and in silico analysis showed that catalytic site domain displayed large deviation and fluctuation upon introduction of these point mutations.
    Conclusion: Overall study provides strong evidence that OTU domain is highly conserved and less prone to mutation whereas point mutations recorded in catalytic domain have affected the stability of protein and were found to be persistent in the large population.
    MeSH term(s) Catalytic Domain/genetics ; Phylogeny ; Mutation ; Point Mutation ; RNA
    Chemical Substances RNA (63231-63-0)
    Language English
    Publishing date 2023-03-06
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1284729-X
    ISSN 0948-5023 ; 1610-2940
    ISSN (online) 0948-5023
    ISSN 1610-2940
    DOI 10.1007/s00894-023-05487-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Addressing diagnostic challenges in suspected contact dermatitis to cosmetics: Can standard series and patient material suffice for patch test?

    Verma, Kaushal / Ahuja, Rhea / Taneja, Neha

    Indian journal of dermatology, venereology and leprology

    2024  , Page(s) 1–2

    Language English
    Publishing date 2024-02-06
    Publishing country United States
    Document type Letter
    ZDB-ID 416068-x
    ISSN 0973-3922 ; 0019-5162 ; 0378-6323
    ISSN (online) 0973-3922
    ISSN 0019-5162 ; 0378-6323
    DOI 10.25259/IJDVL_812_2023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 85: Show issues Location:
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  3. Article ; Online: Analysis of highly frequent point mutations in glycoprotein C, glycoprotein N, and nucleoprotein of CCHFV.

    Kaushal, Neha / Baranwal, Manoj

    Biotechnology and applied biochemistry

    2023  Volume 71, Issue 2, Page(s) 280–294

    Abstract: Crimean-Congo hemorrhagic fever virus (CCHFV) is classified among top 10 priority pathogens by World Health Organization. CCHFV belongs to Bunyaviridae family and negative sense ssRNA genome composed of three RNA segments: L, M, and S. RNA viruses show ... ...

    Abstract Crimean-Congo hemorrhagic fever virus (CCHFV) is classified among top 10 priority pathogens by World Health Organization. CCHFV belongs to Bunyaviridae family and negative sense ssRNA genome composed of three RNA segments: L, M, and S. RNA viruses show higher mutation rate as compared to DNA viruses. To gain deeper understanding of impact of point mutations in CCHFV M and S segment, mutation profiling, homology modeling, and molecular dynamic (MD) simulation were performed. Structural glycoproteins (glycoprotein C [Gc] and glycoprotein N [Gn]) of CCHFV are important for host-virus interaction and genome packaging, whereas CCHFV nucleoprotein (NP) is crucial for viral replication. Hence, current study is focused on evaluation of eight mutations in structural glycoproteins (Gc: 7 and Gn: 1) of M segment and seven mutations in NP of S segment. All these mutations were highly frequent, with mutation frequency between 0.81 and 1.0 and found to be persistent in the recent strains of CCHFV. Solubility analysis predicted that selected point mutations reduce solubility of Gc protein and increase solubility of Gn and NP proteins. MD simulation study deciphered that A1046V and G1158E in Gc protein, I778T in Gn protein, and H195R in NP protein displayed large deviation and fluctuation, and affected intramolecular interactions. In conclusion, we observed that point mutations could impact structure, stability, and host-virus interaction of protein, and might lead to evolution of new strains for better survival and drug resistance.
    MeSH term(s) Hemorrhagic Fever Virus, Crimean-Congo/chemistry ; Hemorrhagic Fever Virus, Crimean-Congo/genetics ; Hemorrhagic Fever Virus, Crimean-Congo/metabolism ; Nucleoproteins/genetics ; Nucleoproteins/metabolism ; Point Mutation ; Glycoproteins/genetics ; Glycoproteins/chemistry ; RNA ; Viral Envelope Proteins
    Chemical Substances glycoprotein gC, herpes simplex virus type 1 ; Nucleoproteins ; Glycoproteins ; RNA (63231-63-0) ; Viral Envelope Proteins
    Language English
    Publishing date 2023-12-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 883433-7
    ISSN 1470-8744 ; 0885-4513
    ISSN (online) 1470-8744
    ISSN 0885-4513
    DOI 10.1002/bab.2540
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1865: Show issues Location:
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  4. Article ; Online: Mutational analysis of catalytic site domain of CCHFV L RNA segment

    Kaushal, Neha / Baranwal, Manoj

    J Mol Model. 2023 Apr., v. 29, no. 4 p.88-88

    2023  

    Abstract: INTRODUCTION: Crimean-Congo haemorrhagic fever virus (CCHFV) has tripartite RNA genome and is endemic in various countries of Asia, Africa and Europe. METHOD: The present study is focused on mutation profiling of CCHFV L segment and phylogenetic ... ...

    Abstract INTRODUCTION: Crimean-Congo haemorrhagic fever virus (CCHFV) has tripartite RNA genome and is endemic in various countries of Asia, Africa and Europe. METHOD: The present study is focused on mutation profiling of CCHFV L segment and phylogenetic clustering of protein dataset into six CCHFV genotypes. RESULTS: Phylogenetic tree rooted with NCBI reference sequence (YP_325663.1) indicated less divergence from genotype III and the sequences belonging to same genotypes have shown less divergence among each other. Mutation frequency at 729 mutated positions was calculated and 563, 49, 33, 46 and 38 amino acid positions were found to be mutated at mutation frequency intervals of 0–0.2, 0.21–0.4, 0.41–0.6, 0.61–0.8 and 0.81–1.0 respectively. Thirty-eight highly frequent mutations (0.81–1.0 interval) were found in all genotypes and mapping in L segment (encoded for RdRp) revealed four mutations (V2074I, I2134T/A, V2148A and Q2695H/R) in catalytic site domain and no mutation in OTU domain. Molecular dynamic simulation and in silico analysis showed that catalytic site domain displayed large deviation and fluctuation upon introduction of these point mutations. CONCLUSION: Overall study provides strong evidence that OTU domain is highly conserved and less prone to mutation whereas point mutations recorded in catalytic domain have affected the stability of protein and were found to be persistent in the large population.
    Keywords Crimean-Congo hemorrhagic fever orthonairovirus ; RNA ; active sites ; amino acids ; computer simulation ; data collection ; genome ; genotype ; models ; mutation ; mutation rate ; mutational analysis ; phylogeny ; Africa ; Asia ; Europe
    Language English
    Dates of publication 2023-04
    Size p. 88.
    Publishing place Springer Berlin Heidelberg
    Document type Article ; Online
    ZDB-ID 1284729-X
    ISSN 0948-5023 ; 1610-2940
    ISSN (online) 0948-5023
    ISSN 1610-2940
    DOI 10.1007/s00894-023-05487-7
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Sclerosing epithelioid fibrosarcoma of the kidney.

    Pathak, Neha / Kakkar, Aanchal / Kaushal, Seema / Batra, Atul

    BMJ case reports

    2023  Volume 16, Issue 6

    Abstract: A woman in her 20s presented with headache and back pain and was found to have a left renal mass with bony metastases. She underwent nephrectomy, and on histopathology was initially diagnosed with stage 4 clear cell sarcoma of the kidney. She underwent ... ...

    Abstract A woman in her 20s presented with headache and back pain and was found to have a left renal mass with bony metastases. She underwent nephrectomy, and on histopathology was initially diagnosed with stage 4 clear cell sarcoma of the kidney. She underwent palliative radiation and chemotherapy; however, the disease progressed and she came to our centre. We started her on second-line chemotherapy and submitted her tissue blocks for review. Due to her age and lack of sclerotic stroma in the tissue, we had our doubts about the diagnosis and hence, tissue sample was submitted for next-generation sequencing (NGS). NGS detected an
    MeSH term(s) Female ; Humans ; Fibrosarcoma/diagnosis ; Fibrosarcoma/therapy ; Nephrectomy ; Bone Neoplasms ; Kidney/pathology
    Language English
    Publishing date 2023-06-09
    Publishing country England
    Document type Case Reports ; Journal Article
    ISSN 1757-790X
    ISSN (online) 1757-790X
    DOI 10.1136/bcr-2022-253447
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: An Innovative Approach for Biocontrol of Meloidogyne incognita in Ginger Using Potential Bacteria Isolated from Indian Himalayas.

    Kaushal, Neha / Sharma, Nivedita / Sharma, Pooja

    Current microbiology

    2023  Volume 80, Issue 12, Page(s) 381

    Abstract: The prevalence of Meloidogyne incognita, a severe root-knot nematode, is alarmingly high in the production of ginger-a main cash crop of Himachal Pradesh, a Himalayan state of India. In order to control this through natural means, the nematicidal ... ...

    Abstract The prevalence of Meloidogyne incognita, a severe root-knot nematode, is alarmingly high in the production of ginger-a main cash crop of Himachal Pradesh, a Himalayan state of India. In order to control this through natural means, the nematicidal potential of plant growth-promoting rhizobacteria (PGPR) against M. incognita was analyzed. This is an effective alternative solution to manage nematode incidence as compared to hazardous chemicals under protected and field cultivation of ginger. In the present study an attempt has been made to isolate, characterize, and identify potential rhizobacteria associated with ginger rhizosphere and endosphere. In total, 169 bacterial isolates were isolated from ginger (Zingiber officinale) rhizosphere and endosphere of 4 different sites of Sirmaur district, screened out for multifarious PGP traits showing positive results. The combined cluster analysis and 16S rRNA genotypic analysis of selected bacterial isolates revealed that Serratia marcescens FS-23, Pseudochrobacter sp. GS-15, Stonotrophomonas pavanii HER-9, Pseudomonas brassicacearum HER-20 and Serratia marcescens IS-2 exhibited highest PGP traits. All tested bacterial isolates were capable of exerting a significant effect on mortality of juvenile M. incognita ranging upto 40-90% in laboratory experiments. Further a consortium of these screened isolates showed 86.67% reduction in gall formation by M. incognita in lab conditions. The remarkable increase to 93.24% with 138.73 q/ha with application of charcoal based bio-formulation of consortium without adding any chemical fertilizer was observed in field trials of Nohradhar of Sirmaur district. An alternative choice as a biocontrol agent as well as for PGP activities, the novel and most robust isolate Serratia marcescens IS-2 had revealed to have a variety of bioactive metabolic products with abilities against nematodes, bacteria, and fungi.
    MeSH term(s) Animals ; Zingiber officinale ; Tylenchoidea/genetics ; RNA, Ribosomal, 16S/genetics ; Bacteria ; Fungi/genetics
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2023-10-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 134238-1
    ISSN 1432-0991 ; 0343-8651
    ISSN (online) 1432-0991
    ISSN 0343-8651
    DOI 10.1007/s00284-023-03496-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1446: Show issues Location:
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  7. Article ; Online: Visible-Light-Mediated Synthesis of α-Ketoamides via Oxidative Amination of 2-Bromoacetophenones Using Eosin Y as a Photoredox Catalyst.

    Kishor, Kaushal / Prabhakar, Neha Sharma / Singh, Krishna Nand

    Chemistry, an Asian journal

    2023  Volume 18, Issue 19, Page(s) e202300669

    Abstract: An oxidative amination of 2-bromoacetophenones has been accomplished to provide α-ketoamides by using photoredox catalysis with air as oxidant. The reactants are readily accessible, and the method is endowed with broad substrate scope and good functional ...

    Abstract An oxidative amination of 2-bromoacetophenones has been accomplished to provide α-ketoamides by using photoredox catalysis with air as oxidant. The reactants are readily accessible, and the method is endowed with broad substrate scope and good functional group tolerance. The practicality of the approach is also shown by a gram-scale reaction.
    Language English
    Publishing date 2023-09-05
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2233006-9
    ISSN 1861-471X ; 1861-4728
    ISSN (online) 1861-471X
    ISSN 1861-4728
    DOI 10.1002/asia.202300669
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Computational design of immunogenic peptide constructs comprising multiple human leukocyte antigen restricted dengue virus envelope epitopes.

    Kaushal, Neha / Jain, Sahil / Baranwal, Manoj

    Journal of molecular recognition : JMR

    2022  Volume 35, Issue 9, Page(s) e2961

    Abstract: Dengue virus (DENV) is endemic in 100 countries with the ability to impact nearly 50% of world population. DENV envelope (E) protein is responsible for viral attachment to host cells and has been target of various countermeasure development efforts. The ... ...

    Abstract Dengue virus (DENV) is endemic in 100 countries with the ability to impact nearly 50% of world population. DENV envelope (E) protein is responsible for viral attachment to host cells and has been target of various countermeasure development efforts. The current study focuses on a consensus computational approach to identify cross-reactive, immunogenic DENV-2 E peptides displaying promiscuity with a wide array of human leukocyte antigen (HLA) molecules. Four conserved peptides (FP-1, FP-2, FP-3 and FP-4) containing multiple CD8
    MeSH term(s) Dengue ; Epitopes, T-Lymphocyte/chemistry ; HLA Antigens/chemistry ; Histocompatibility Antigens Class II ; Humans ; Molecular Docking Simulation ; Peptides/chemistry ; Viral Envelope
    Chemical Substances Epitopes, T-Lymphocyte ; HLA Antigens ; Histocompatibility Antigens Class II ; Peptides
    Language English
    Publishing date 2022-05-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 1015084-5
    ISSN 1099-1352 ; 0952-3499
    ISSN (online) 1099-1352
    ISSN 0952-3499
    DOI 10.1002/jmr.2961
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  9. Article ; Online: A Colossal Rhinolith

    Neha Shakrawal / Bikram Choudhury / Kapil Soni / Darwin Kaushal

    Bengal Journal of Otolaryngology and Head Neck Surgery, Vol 29, Iss

    2021  Volume 1

    Abstract: Introduction Rhinolith is a calcic deposition formed by mineralization of salts in an impacted nasal foreign body. It is a benign condition that can be troublesome owing to its size and extent of impact. Case Report A 35-year-old man with a history of ... ...

    Abstract Introduction Rhinolith is a calcic deposition formed by mineralization of salts in an impacted nasal foreign body. It is a benign condition that can be troublesome owing to its size and extent of impact. Case Report A 35-year-old man with a history of right nasal obstruction with occasional foul-smelling discharge and right eye pain since ten years was diagnosed with rhinolith. During its removal, it was three times bigger than what could be visualized in nasal endoscopy. Conclusion Proper history, examination, and a high suspicion can clinch the diagnosis in almost all cases. A rigid diagnostic nasendoscopy is an important tool in the diagnosis. The treatment of choice is endoscopic removal under local or general anaesthesia.
    Keywords Rhinolith ; Foreign Bodies ; Nose ; Nasal Obstruction ; Medicine ; R ; Otorhinolaryngology ; RF1-547
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher The Association of Otolaryngologists of India, West Bengal
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Luminescence nanothermometry using a trivalent lanthanide co-doped perovskite.

    Singh, Prashansha / Jain, Neha / Shukla, Shraddha / Tiwari, Anish Kumar / Kumar, Kaushal / Singh, Jai / Pandey, Avinash C

    RSC advances

    2023  Volume 13, Issue 5, Page(s) 2939–2948

    Abstract: This study investigates in detail the laser-mediated upconversion emission and temperature-sensing capability of ( ... ...

    Abstract This study investigates in detail the laser-mediated upconversion emission and temperature-sensing capability of (Ca
    Language English
    Publishing date 2023-01-18
    Publishing country England
    Document type Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/d2ra05935e
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