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Article ; Online: Prevalence of massively diluted bone marrow cell samples aspirated from patients with myelodysplastic syndromes (MDS) or suspected of MDS: A retrospective analysis of nationwide samples in Japan.

Ogata, Kiyoyuki / Mochimaru, Yuto / Kasai, Nana / Sei, Kazuma / Kawahara, Naoya / Ogata, Mika / Yamamoto, Yumi

2024

Abstract: Bone marrow (BM) examination is a key element in the diagnosis and prognostic grading of myelodysplastic syndromes (MDSs), and obtaining adequate BM cell samples is critical for accurate test results. Massive haemodilution of aspirated BM samples is a ... ...

Abstract	Bone marrow (BM) examination is a key element in the diagnosis and prognostic grading of myelodysplastic syndromes (MDSs), and obtaining adequate BM cell samples is critical for accurate test results. Massive haemodilution of aspirated BM samples is a well-known problem; however, its incidence in patients with MDS has not been well studied. We report the first study to examine the incidence of massive haemodilution in nationwide BM samples aspirated from patients diagnosed with or suspected of MDS in Japan. Among 283 cases available for analysis, BM smears from 92 cases (32.5%) were hypospicular (massively haemodiluted) and, particularly, no BM particles were observed in 52 cases (18.4%). Regarding hypospicular cases, we examined how the doctors in charge interpreted the BM smears of their patients. In only 19 of 92 cases (20.7%), doctors realised that the BM smears were haemodiluted. Furthermore, the BM biopsy, which can help diagnose hypospicular cases, was oftentimes not performed when the haemodilution was overlooked by doctors (not performed in 50 of 73 such cases). These real-world data highlight that not only researchers who are working to improve diagnostic tests but also clinicians who perform and use diagnostic tests must realise this common and potentially critical problem.
Language	English
Publishing date	2024-04-08
Publishing country	England
Document type	Journal Article
ZDB-ID	80077-6
ISSN	1365-2141 ; 0007-1048
ISSN (online)	1365-2141
ISSN	0007-1048
DOI	10.1111/bjh.19447
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Article ; Online: WT1 Expression in Patients with Myelodysplastic Syndromes: A Variety of Possibilities in Future Clinical Practice.

Ogata, Kiyoyuki

Acta haematologica

2017 Volume 137, Issue 1, Page(s) 30–31

MeSH term(s)	Biomarkers/metabolism ; Bone Marrow Cells/immunology ; Bone Marrow Cells/pathology ; Gene Expression ; Humans ; Myelodysplastic Syndromes/diagnosis ; Myelodysplastic Syndromes/genetics ; Myelodysplastic Syndromes/immunology ; Myelodysplastic Syndromes/pathology ; Prognosis ; RNA, Messenger/genetics ; RNA, Messenger/immunology ; WT1 Proteins/genetics ; WT1 Proteins/immunology
Chemical Substances	Biomarkers ; RNA, Messenger ; WT1 Proteins ; WT1 protein, human
Language	English
Publishing date	2017
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	80008-9
ISSN	1421-9662 ; 0001-5792
ISSN (online)	1421-9662
ISSN	0001-5792
DOI	10.1159/000449363
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Article ; Online: Myeloblasts transition to megakaryoblastic immunophenotypes over time in some patients with myelodysplastic syndromes.

Ogata, Kiyoyuki / Mochimaru, Yuto / Sei, Kazuma / Kawahara, Naoya / Ogata, Mika / Yamamoto, Yumi

PloS one

2023 Volume 18, Issue 9, Page(s) e0291662

Abstract: Objectives: In myelodysplastic syndromes (MDS), neoplastic myeloblast (CD34+CD13+CD33+ cells) numbers often increase over time, leading to secondary acute myeloid leukemia (AML). In recent studies, blasts in some MDS patients have been found to express ... ...

Abstract	Objectives: In myelodysplastic syndromes (MDS), neoplastic myeloblast (CD34+CD13+CD33+ cells) numbers often increase over time, leading to secondary acute myeloid leukemia (AML). In recent studies, blasts in some MDS patients have been found to express a megakaryocyte-lineage molecule, CD41, and such patients show extremely poor prognosis. This is the first study to evaluate whether myeloblasts transition to CD41+ blasts over time and to investigate the detailed immunophenotypic features of CD41+ blasts in MDS. Methods: We performed a retrospective cohort study, in which time-dependent changes in blast immunophenotypes were analyzed using multidimensional flow cytometry (MDF) in 74 patients with MDS and AML (which progressed from MDS). Results: CD41+ blasts (at least 20% of CD34+ blasts expressing CD41) were detected in 12 patients. In five of these 12 patients, blasts were CD41+ from the first MDF analysis. In the other seven patients, myeloblasts (CD34+CD33+CD41- cells) transitioned to megakaryoblasts (CD34+CD41+ cells) over time, which was often accompanied by disease progression (including leukemic transformation). These CD41+ patients were more frequently observed among patients with monosomal and complex karyotypes. CD41+ blasts were negative for the erythroid antigen, CD235a, and positive for CD33 in all cases, but CD33 expression levels were lower in three cases when compared with CD34+CD41- blasts. Among the five CD41+ patients who underwent extensive immunophenotyping, CD41+ blasts all expressed CD61, but two cases had reduced CD42b expression, three had reduced/absent CD13 expression, and three also expressed CD7. Conclusions: Myeloblasts become megakaryoblastic over time in some MDS patients, and examining the megakaryocyte lineage (not only as a diagnostic work-up but also as follow-up) is needed to detect CD41+ MDS. The immunophenotypic features revealed in this study may have diagnostic relevance for CD41+ MDS patients.
MeSH term(s)	Humans ; Granulocyte Precursor Cells ; Immunophenotyping ; Megakaryocyte Progenitor Cells ; Retrospective Studies ; Antigens, CD34 ; Myelodysplastic Syndromes
Chemical Substances	Antigens, CD34
Language	English
Publishing date	2023-09-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0291662
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Article ; Online: Myeloblasts transition to megakaryoblastic immunophenotypes over time in some patients with myelodysplastic syndromes.

Kiyoyuki Ogata / Yuto Mochimaru / Kazuma Sei / Naoya Kawahara / Mika Ogata / Yumi Yamamoto

PLoS ONE, Vol 18, Iss 9, p e

2023 Volume 0291662

Abstract: Objectives In myelodysplastic syndromes (MDS), neoplastic myeloblast (CD34+CD13+CD33+ cells) numbers often increase over time, leading to secondary acute myeloid leukemia (AML). In recent studies, blasts in some MDS patients have been found to express a ... ...

Abstract	Objectives In myelodysplastic syndromes (MDS), neoplastic myeloblast (CD34+CD13+CD33+ cells) numbers often increase over time, leading to secondary acute myeloid leukemia (AML). In recent studies, blasts in some MDS patients have been found to express a megakaryocyte-lineage molecule, CD41, and such patients show extremely poor prognosis. This is the first study to evaluate whether myeloblasts transition to CD41+ blasts over time and to investigate the detailed immunophenotypic features of CD41+ blasts in MDS. Methods We performed a retrospective cohort study, in which time-dependent changes in blast immunophenotypes were analyzed using multidimensional flow cytometry (MDF) in 74 patients with MDS and AML (which progressed from MDS). Results CD41+ blasts (at least 20% of CD34+ blasts expressing CD41) were detected in 12 patients. In five of these 12 patients, blasts were CD41+ from the first MDF analysis. In the other seven patients, myeloblasts (CD34+CD33+CD41- cells) transitioned to megakaryoblasts (CD34+CD41+ cells) over time, which was often accompanied by disease progression (including leukemic transformation). These CD41+ patients were more frequently observed among patients with monosomal and complex karyotypes. CD41+ blasts were negative for the erythroid antigen, CD235a, and positive for CD33 in all cases, but CD33 expression levels were lower in three cases when compared with CD34+CD41- blasts. Among the five CD41+ patients who underwent extensive immunophenotyping, CD41+ blasts all expressed CD61, but two cases had reduced CD42b expression, three had reduced/absent CD13 expression, and three also expressed CD7. Conclusions Myeloblasts become megakaryoblastic over time in some MDS patients, and examining the megakaryocyte lineage (not only as a diagnostic work-up but also as follow-up) is needed to detect CD41+ MDS. The immunophenotypic features revealed in this study may have diagnostic relevance for CD41+ MDS patients.
Keywords	Medicine ; R ; Science ; Q
Subject code	610
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
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Article ; Online: Clinical, immunophenotypic, and cytogenetic characteristics of high-grade myelodysplastic syndromes with CD41-positive progenitor cells.

Ogata, Kiyoyuki / Sei, Kazuma / Kawahara, Naoya / Ogata, Mika / Yamamoto, Yumi

Cytometry. Part B, Clinical cytometry

2021 Volume 104, Issue 1, Page(s) 98–107

Abstract: Background: Patients with myelodysplastic syndromes (MDS) with progenitors expressing CD41 (CD41+ MDS) showed a poor prognosis in a previous study but their detailed characteristics remain unclear.: Methods: One hundred thirty-seven subjects at our ... ...

Abstract	Background: Patients with myelodysplastic syndromes (MDS) with progenitors expressing CD41 (CD41+ MDS) showed a poor prognosis in a previous study but their detailed characteristics remain unclear. Methods: One hundred thirty-seven subjects at our institution were diagnosed with excess blasts (EB)-1, EB-2, and acute myeloid leukemia with a low blast count (20%-30%). The immunophenotypes of progenitor cells in their bone marrow (BM) were determined by CD45-gating flow cytometry. A false-positive reaction to CD41 was eliminated by examining the flow cytometry data of lymphocytes and monocytes in addition to progenitors and by examining CD42b in histological sections. The characteristics were compared between CD41+ and CD41- MDS patients. Results: Forty-three patients (31%) were CD41+. Additionally, 91% of the CD41+ MDS patients were very high-risk defined by the Revised International Prognostic Score System, which was higher than in patients with CD41- MDS (p = 0.015). Approximately 60% of the CD41+ MDS patients had a monosomal karyotype and very poor cytogenetics, which was higher than in CD41- MDS patients (p < 0.001). Normal cytogenetics was less common in CD41+ patients (p = 0.0016). Blasts with bleb formation were more abundant in CD41+ MDS patients (p = 0.026). All CD41+ MDS patients were positive for CD13 and were mostly positive for CD33. The frequency of aberrant expression of other antigens on progenitors was similar between CD41+ and CD41- MDS patients. Conclusions: We determined clinical, immunophenotypic, and cytogenetic characteristics of CD41+ MDS patients. Further studies are needed to improve the survival of these patients.
MeSH term(s)	Humans ; Flow Cytometry ; Myelodysplastic Syndromes/diagnosis ; Myelodysplastic Syndromes/genetics ; Myelodysplastic Syndromes/pathology ; Bone Marrow/pathology ; Karyotyping ; Stem Cells/pathology
Language	English
Publishing date	2021-12-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	2099657-3
ISSN	1552-4957 ; 1552-4949 ; 0196-4763
ISSN (online)	1552-4957
ISSN	1552-4949 ; 0196-4763
DOI	10.1002/cyto.b.22052
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Article ; Online: Clinical significance of CD41-positive blasts in association with a monosomal karyotype in patients with myelodysplastic syndrome treated with azacitidine.

Ogata, Kiyoyuki / Sei, Kazuma / Kawahara, Naoya / Yamamoto, Yumi

British journal of haematology

2020 Volume 189, Issue 4, Page(s) e144–e147

MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antimetabolites, Antineoplastic/pharmacology ; Antimetabolites, Antineoplastic/therapeutic use ; Azacitidine/pharmacology ; Azacitidine/therapeutic use ; Female ; Humans ; Karyotype ; Male ; Middle Aged ; Myelodysplastic Syndromes/drug therapy ; Myelodysplastic Syndromes/immunology ; Platelet Membrane Glycoprotein IIb/immunology ; Young Adult
Chemical Substances	Antimetabolites, Antineoplastic ; Platelet Membrane Glycoprotein IIb ; Azacitidine (M801H13NRU)
Language	English
Publishing date	2020-03-04
Publishing country	England
Document type	Letter
ZDB-ID	80077-6
ISSN	1365-2141 ; 0007-1048
ISSN (online)	1365-2141
ISSN	0007-1048
DOI	10.1111/bjh.16565
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Article ; Online: Flow cytometry will be a routine tool in clinical practice in myelodysplastic syndromes: a real story.

Ogata, Kiyoyuki

Leukemia research

2011 Volume 35, Issue 7, Page(s) 848–849

MeSH term(s)	Flow Cytometry/methods ; Flow Cytometry/utilization ; Humans ; Myelodysplastic Syndromes/diagnosis ; Prognosis ; Survival Rate
Language	English
Publishing date	2011-07
Publishing country	England
Document type	Comment ; Editorial
ZDB-ID	752396-8
ISSN	1873-5835 ; 0145-2126
ISSN (online)	1873-5835
ISSN	0145-2126
DOI	10.1016/j.leukres.2011.03.018
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Article: [New horizons in MDS: flow cytometric diagnosis and a role of immunomodulatory molecules in disease progression].

Ogata, Kiyoyuki

Rinsho ketsueki] The Japanese journal of clinical hematology

2011 Volume 52, Issue 3, Page(s) 106–110

MeSH term(s)	Antigens, CD ; B7-H1 Antigen ; Disease Progression ; Flow Cytometry/methods ; Humans ; Interleukin-2 Receptor alpha Subunit ; Myelodysplastic Syndromes/diagnosis ; Myelodysplastic Syndromes/immunology
Chemical Substances	Antigens, CD ; B7-H1 Antigen ; CD274 protein, human ; Interleukin-2 Receptor alpha Subunit
Language	Japanese
Publishing date	2011-03
Publishing country	Japan
Document type	Journal Article ; Review
ZDB-ID	390900-1
ISSN	0485-1439
ISSN	0485-1439
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Article: Diagnostic flow cytometry for low-grade myelodysplastic syndromes.

Ogata, Kiyoyuki

Hematological oncology

2008 Volume 26, Issue 4, Page(s) 193–198

Abstract: It has long been considered that flow cytometry (FCM) has little role in clinical practice in the diagnosis of myelodysplastic syndromes (MDS). However, recent advances in the analytical method and knowledge of MDS FCM are changing this stereotype. This ... ...

Abstract	It has long been considered that flow cytometry (FCM) has little role in clinical practice in the diagnosis of myelodysplastic syndromes (MDS). However, recent advances in the analytical method and knowledge of MDS FCM are changing this stereotype. This paper reviews the concept and current status of FCM in the diagnosis of low-grade MDS. The diagnosis of low-grade MDS in the absence of ringed sideroblasts and chromosomal aberration is not always straightforward, and a report from a recent international working conference has proposed FCM as an adjunctive diagnostic test for such cases. Currently, only a limited number of laboratories are applying FCM to the diagnosis of MDS. Furthermore, standard analytical methods in FCM for MDS have not been established, and no single FCM parameter is sufficiently sensitive and specific to make the diagnosis of MDS. To establish MDS FCM as a widely accepted, dependable diagnostic tool, prospective studies should increase flow parameters that can be analysed reproducibly and determine their sensitivity and specificity, either alone or in combination. CD34+ cell-related parameters that are applicable for diagnosing low-grade MDS in many laboratories are introduced here.
MeSH term(s)	Antigens, CD34/analysis ; Flow Cytometry/methods ; Forecasting ; Granulocyte Precursor Cells/metabolism ; Humans ; Leukocyte Common Antigens/analysis ; Myelodysplastic Syndromes/diagnosis ; Pattern Recognition, Visual ; Precursor Cells, B-Lymphoid/metabolism ; Reproducibility of Results ; Sensitivity and Specificity
Chemical Substances	Antigens, CD34 ; Leukocyte Common Antigens (EC 3.1.3.48)
Language	English
Publishing date	2008-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	604884-5
ISSN	1099-1069 ; 0278-0232
ISSN (online)	1099-1069
ISSN	0278-0232
DOI	10.1002/hon.857
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Article: Myelodysplastic syndromes: recent progress in diagnosis and understanding of their pathophysiology.

Ogata, Kiyoyuki

Journal of Nippon Medical School = Nippon Ika Daigaku zasshi

2006 Volume 73, Issue 6, Page(s) 300–307

Abstract: Myelodysplastic syndromes (MDS) are common malignant disorders with a poor prognosis. MDS are a group of highly heterogeneous disorders but show certain universal findings including a high incidence in the elderly population, cytopenia, dysplastic ... ...

Abstract	Myelodysplastic syndromes (MDS) are common malignant disorders with a poor prognosis. MDS are a group of highly heterogeneous disorders but show certain universal findings including a high incidence in the elderly population, cytopenia, dysplastic myeloid cells, and frequent transformation to acute myeloid leukemia. Until recently, the vast majority of MDS patients were treated with supportive therapy alone, such as transfusions. Allogeneic stem cell transplantation (SCT) has the potential for cure, although due to the age and comorbidity of MDS patients, the role of allogeneic SCT in MDS has been limited. Recently, research in MDS has shown substantial advances that have deepened our understanding of MDS pathophysiology and changed our approach to MDS patients. This review touches on some recent developments in the diagnosis and pathophysiology of MDS.
MeSH term(s)	Apoptosis ; Cytogenetics ; Epigenesis, Genetic ; Flow Cytometry ; Humans ; Immunocompetence ; Leukemia, Myeloid, Acute ; Myelodysplastic Syndromes/classification ; Myelodysplastic Syndromes/diagnosis ; Myelodysplastic Syndromes/etiology ; T-Lymphocytes/immunology
Language	English
Publishing date	2006-11-15
Publishing country	Japan
Document type	Journal Article ; Review
ZDB-ID	2091563-9
ISSN	1347-3409 ; 1345-4676
ISSN (online)	1347-3409
ISSN	1345-4676
DOI	10.1272/jnms.73.300
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