LIVIVO - Search results -

Search results

Result 1 - 10 of total 25

Article: Offbeat mice.

Gaussin, Vinciane

The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology

2004 Volume 280, Issue 2, Page(s) 1022–1026

Abstract: This review summarizes the recent advances in understanding the development and function of the cardiac conduction system using genetically modified mice. Null mice for different cardiac connexins confirmed their suspected roles in cardiac conduction and, ...

Abstract	This review summarizes the recent advances in understanding the development and function of the cardiac conduction system using genetically modified mice. Null mice for different cardiac connexins confirmed their suspected roles in cardiac conduction and, in addition, unraveled unexpected roles in cardiac morphogenesis. Genetically modified mice with LacZ-labeled conduction system cells are indispensable tools to the further understanding of the mechanisms governing the development of this system in the mammalian heart. Mouse models also addressed the role and contribution of specific signaling molecules, such as PRKAG2, Nkx2.5, ALK3, and Tbx5, in the development of the cardiac conduction system and the pathogenesis of cardiac dysfunction in humans.
MeSH term(s)	Animals ; Arrhythmias, Cardiac/metabolism ; Arrhythmias, Cardiac/pathology ; Connexins/genetics ; Connexins/physiology ; Death, Sudden, Cardiac/etiology ; Gene Expression Regulation, Developmental ; Heart Block/metabolism ; Heart Conduction System/embryology ; Heart Conduction System/physiology ; Humans ; Models, Animal ; Myocytes, Cardiac/metabolism
Chemical Substances	Connexins
Language	English
Publishing date	2004-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2103089-3
ISSN	1552-4884 ; 0003-276X
ISSN	1552-4884 ; 0003-276X
DOI	10.1002/ar.a.20074
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ub I Zs 65/A: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Relationships between melanocytes, mechanical properties and extracellular matrix composition in mouse heart valves.

Carneiro, Flavia / Kruithof, Boudewijn Pt / Balani, Kanthesh / Agarwal, Arvind / Gaussin, Vinciane / Kos, Lidia

Journal of long-term effects of medical implants

2015 Volume 25, Issue 1-2, Page(s) 17–26

Abstract: Heart valves are complex structures composed of organized layers of extracellular matrix, and interstitial and overlying endothelial cells. In this article, we present the specific localization of a population of melanocytes within the murine heart ... ...

Abstract	Heart valves are complex structures composed of organized layers of extracellular matrix, and interstitial and overlying endothelial cells. In this article, we present the specific localization of a population of melanocytes within the murine heart valves at ages important for their post-natal development. In all stages analyzed in our study, melanocytes were found in high numbers populating the atrial aspect of the tricuspid and mitral leaflets. The pulmonary valve did not present melanocytes. To characterize a putative role for the valve melanocytes, the dynamic nanomechanical properties of tricuspid leaftets containing large numbers or no melanocytes were measured. The stiffness coefficient of hyperpigmented leaflets was higher (11.5 GPa) than the ones from wild-type (7.5 GPa) and hypopigmented (5.5 GPa) leaflets. These results suggest that melanocytes may contribute to the mechanical properties of the heart valves. The arrangement of extracellular matrix molecules such as Collagen I and Versican B is responsible for the mechanical characteristics of the leaflets. Melanocytes were found to reside primarily in areas of Versican B expression. The patterns of expression of Collagen I and Versican B were not, however, disrupted in hyper or hypopigmented leaflets. Melanocytes may affect other extracellular matrix molecules to alter the valves' microenvironment.
MeSH term(s)	Animals ; Biomechanical Phenomena ; Cells, Cultured ; Disease Models, Animal ; Extracellular Matrix/metabolism ; Heart Valve Diseases/pathology ; Heart Valve Diseases/physiopathology ; Heart Valves/metabolism ; Heart Valves/pathology ; Heart Valves/physiopathology ; Melanocytes/metabolism ; Melanocytes/pathology ; Mice ; Mice, Inbred C57BL
Language	English
Publishing date	2015-05-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	1073166-0
ISSN	1940-4379 ; 1050-6934
ISSN (online)	1940-4379
ISSN	1050-6934
DOI	10.1615/jlongtermeffmedimplants.2015011748
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3217: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Myostatin, the cardiac chalone of insulin-like growth factor-1.

Gaussin, Vinciane / Depre, Christophe

Cardiovascular research

2005 Volume 68, Issue 3, Page(s) 347–349

MeSH term(s)	Acromegaly/metabolism ; Acromegaly/pathology ; Animals ; Cardiomegaly/metabolism ; Cardiomegaly/pathology ; Chalones/metabolism ; Humans ; Insulin-Like Growth Factor I/metabolism ; Mitogen-Activated Protein Kinase Kinases/metabolism ; Myocardial Infarction/metabolism ; Myocardial Infarction/pathology ; Myocardium/metabolism ; Myocardium/pathology ; Myostatin ; Organ Size ; Signal Transduction/physiology ; Transforming Growth Factor beta/metabolism
Chemical Substances	Chalones ; MSTN protein, human ; Myostatin ; Transforming Growth Factor beta ; Insulin-Like Growth Factor I (67763-96-6) ; Mitogen-Activated Protein Kinase Kinases (EC 2.7.12.2)
Language	English
Publishing date	2005-12-01
Publishing country	England
Document type	Comment ; Editorial
ZDB-ID	80340-6
ISSN	1755-3245 ; 0008-6363
ISSN (online)	1755-3245
ISSN	0008-6363
DOI	10.1016/j.cardiores.2005.09.007
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 565: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Atrioventricular valve development during late embryonic and postnatal stages involves condensation and extracellular matrix remodeling.

Kruithof, Boudewijn P T / Krawitz, Steven A / Gaussin, Vinciane

Developmental biology

2007 Volume 302, Issue 1, Page(s) 208–217

Abstract: Although the signaling molecules regulating the early stages of valvular development have been well described, little is known on the late steps leading to mature fibrous leaflets. We hypothesized that atrioventricular (AV) valve development continues ... ...

Abstract	Although the signaling molecules regulating the early stages of valvular development have been well described, little is known on the late steps leading to mature fibrous leaflets. We hypothesized that atrioventricular (AV) valve development continues after birth to adjust to the postnatal maturation of the heart. By doing a systematic analysis of the AV valves of mice from embryonic day (E) 15.5 to 8 weeks old, we identified key developmental steps that map the maturation process of embryonic cushion-like leaflets into adult stress-resistant valves. Condensation of the mesenchymal cells occurred between E15.5 and E18.5 and was accompanied by increased cellular proliferation and adhesion. Cellular proliferation also contributed transiently to the concomitant elongation of the leaflets. Patterning of the extracellular matrix (ECM) proteins along the AV axis was achieved 1 week after birth, with the differentiation of two reciprocal structural regions, glycosaminoglycans and versican at the atrial side, and densely packed collagen fibers at the ventricular side. Formation and remodeling of the nodular thickenings at the closure points of the leaflets occurred between N4.5 and N11.5. In conclusion, AV valve development during late embryonic and postnatal stages includes condensation, elongation, formation of nodular thickenings, and remodeling of tension-resistant ECM proteins.
MeSH term(s)	Animals ; Animals, Newborn ; Cell Count ; Cell Proliferation ; Embryo, Mammalian/metabolism ; Extracellular Matrix/metabolism ; Extracellular Matrix Proteins/metabolism ; Heart/embryology ; Heart/growth & development ; Heart Septum/embryology ; Heart Septum/growth & development ; Heart Valves/embryology ; Heart Valves/growth & development ; Heart Valves/metabolism ; Mesoderm/cytology ; Mice ; Mice, Inbred Strains
Chemical Substances	Extracellular Matrix Proteins
Language	English
Publishing date	2007-02-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1114-9
ISSN	1095-564X ; 0012-1606
ISSN (online)	1095-564X
ISSN	0012-1606
DOI	10.1016/j.ydbio.2006.09.024
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 508: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Activation of the bone morphogenetic protein receptor by H11kinase/Hsp22 promotes cardiac cell growth and survival.

Sui, Xiangzhen / Li, Dan / Qiu, Hongyu / Gaussin, Vinciane / Depre, Christophe

Circulation research

2009 Volume 104, Issue 7, Page(s) 887–895

Abstract: H11 kinase/Hsp22 (H11K) is a chaperone promoting cardiac cell growth and survival through the activation of Akt, a downstream effector of phosphatidylinositol 3-kinase (PI3K). In this study, we tested whether H11K-induced activation of the PI3K/Akt ... ...

Abstract	H11 kinase/Hsp22 (H11K) is a chaperone promoting cardiac cell growth and survival through the activation of Akt, a downstream effector of phosphatidylinositol 3-kinase (PI3K). In this study, we tested whether H11K-induced activation of the PI3K/Akt pathway is mediated by the bone morphogenetic protein (BMP) signaling, both in a transgenic mouse model with cardiac-specific overexpression of H11K and in isolated cardiac myocytes. Microarrays in hearts from transgenic compared to wild-type mice showed an upregulation of the BMP receptors Alk3 and BMPR-II, and of their ligand BMP4 (P<0.01 versus wild type). Activation of the BMP pathway in transgenic mice was confirmed by increased phosphorylation of the "canonical" BMP effectors Smad 1/5/8 (P<0.01 versus wild type). In isolated myocytes, adenovirus-mediated overexpression of H11K was accompanied by a significant (P<0.01) increase in PI3K activity, phospho-Akt, Smad 1/5/8 phosphorylation and [(3)H]phenylalanine incorporation, and by a 70% reduction in H(2)O(2)-mediated apoptosis. All these effects were abolished by the BMP antagonist noggin. In presence of BMP4, Smad 1/5/8 phosphorylation was enhanced by 5-fold on H11K overexpression but decreased by 3-fold on H11K knockdown (P<0.01 versus control), showing that H11K potentiates the BMP signaling. In pull-down experiments, H11K increased both the association of Alk3 and BMPR-II together, and their interaction with the transforming growth factor-beta-activated kinase (TAK)1, a "noncanonical" mediator of the BMP receptor signaling. TAK1 inhibition prevented H11K-mediated activation of Akt. Therefore, potentiation of the BMP receptor by H11K promotes an activation of the PI3K/Akt pathway mediated by TAK1, which dictates the physiological effects of H11K on cardiac cell growth and survival.
MeSH term(s)	Animals ; Animals, Newborn ; Apoptosis ; Bone Morphogenetic Protein 4/metabolism ; Bone Morphogenetic Protein Receptors/genetics ; Bone Morphogenetic Protein Receptors/metabolism ; Bone Morphogenetic Protein Receptors, Type I/metabolism ; Bone Morphogenetic Protein Receptors, Type II/metabolism ; Carrier Proteins/metabolism ; Cell Proliferation ; Cell Survival ; Cells, Cultured ; Heat-Shock Proteins/genetics ; Heat-Shock Proteins/metabolism ; Humans ; MAP Kinase Kinase Kinases/metabolism ; Male ; Mice ; Mice, Transgenic ; Molecular Chaperones ; Myocytes, Cardiac/enzymology ; Myocytes, Cardiac/pathology ; Phosphatidylinositol 3-Kinases/metabolism ; Phosphorylation ; Protein-Serine-Threonine Kinases/genetics ; Protein-Serine-Threonine Kinases/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Rats ; Signal Transduction ; Smad Proteins, Receptor-Regulated/metabolism ; Transduction, Genetic
Chemical Substances	Bmp4 protein, mouse ; Bone Morphogenetic Protein 4 ; Carrier Proteins ; HSPB8 protein, human ; Heat-Shock Proteins ; Molecular Chaperones ; Smad Proteins, Receptor-Regulated ; noggin protein (148294-77-3) ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; MAP Kinase Kinase Kinases (EC 2.7.11.25) ; MAP kinase kinase kinase 7 (EC 2.7.11.25) ; Bmpr1a protein, mouse (EC 2.7.11.30) ; Bmpr2 protein, mouse (EC 2.7.11.30) ; Bone Morphogenetic Protein Receptors (EC 2.7.11.30) ; Bone Morphogenetic Protein Receptors, Type I (EC 2.7.11.30) ; Bone Morphogenetic Protein Receptors, Type II (EC 2.7.11.30)
Language	English
Publishing date	2009-02-26
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	80100-8
ISSN	1524-4571 ; 0009-7330 ; 0931-6876
ISSN (online)	1524-4571
ISSN	0009-7330 ; 0931-6876
DOI	10.1161/CIRCRESAHA.108.192328
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ui IV Zs.70: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Ui IV Zs.60: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Design of a miniature tissue culture system to culture mouse heart valves.

Lieber, Samuel C / Kruithof, Boudewijn P T / Aubry, Nadine / Vatner, Stephen F / Gaussin, Vinciane

Annals of biomedical engineering

2010 Volume 38, Issue 3, Page(s) 674–682

Abstract: Valvular heart disease is a leading cause of morbidity and mortality in adults but little is known about the underlying etiology. A better understanding of the genetic and hemodynamic mechanisms involved in growth and remodeling of heart valves during ... ...

Abstract	Valvular heart disease is a leading cause of morbidity and mortality in adults but little is known about the underlying etiology. A better understanding of the genetic and hemodynamic mechanisms involved in growth and remodeling of heart valves during physiological and pathological conditions is needed for a better understanding of valvular heart disease. Here, we report the design of a miniature tissue culture system (MTCS) that allows the culture of mitral valves from perinatal to adult mice. The design of the MTCS is novel in that fine positioning and cannulation can be conducted with hearts of different sizes (perinatal to adult). Perfusion of the heart and hence, culture of the mitral valve in its natural position, occurs in a hydraulically sealed culture bath environment. Using the MTCS, we successfully cultured the mitral valve of adult mouse hearts for 3 days. Histological analysis indicated that the cultured valves remained viable and their extracellular matrix organization was similar to age-matched native valves. Gene expression could also be modified in cultured valves by perfusion with medium containing beta-galactosidase-expressing adenovirus. Thus, the MTCS is a new tool to study the genetic and hemodynamic mechanisms underlying the three-dimensional organization of the heart valves, which could provide insights in the pathology of valvular heart disease and be used in animal models for the development of tissue-engineered heart valves.
MeSH term(s)	Animals ; Bioprosthesis/veterinary ; Cells, Cultured ; Equipment Design ; Equipment Failure Analysis ; Heart Valve Prosthesis/veterinary ; Mice ; Miniaturization ; Myocytes, Cardiac/cytology ; Myocytes, Cardiac/physiology ; Organ Culture Techniques/instrumentation ; Organ Culture Techniques/methods ; Perfusion/instrumentation ; Perfusion/veterinary
Language	English
Publishing date	2010-01-23
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	185984-5
ISSN	1573-9686 ; 0191-5649 ; 0090-6964
ISSN (online)	1573-9686
ISSN	0191-5649 ; 0090-6964
DOI	10.1007/s10439-010-9922-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1018: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Remodeling of the myocardium in early trabeculation and cardiac valve formation; a role for TGFβ2.

Kruithof, Boudewijn P T / Kruithof-De-Julio, Marianna / Poelmann, Robert E / Gittenberger-De-Groot, Adriana C / Gaussin, Vinciane / Goumans, Marie-José

The International journal of developmental biology

2013 Volume 57, Issue 11-12, Page(s) 853–863

Abstract: Trabeculation and the formation of the leaflets of the mitral and tricuspid valves both involve remodeling of the embryonic myocardium. The nature and possible connection of these myocardial remodeling processes, however, are unclear. Therefore, we ... ...

Abstract	Trabeculation and the formation of the leaflets of the mitral and tricuspid valves both involve remodeling of the embryonic myocardium. The nature and possible connection of these myocardial remodeling processes, however, are unclear. Therefore, we examined the morphogenesis of the early ventricular and atrioventricular (AV) myocardium and report for the first time that the formation of the early trabeculae and the positioning of the valve primordia (endocardial cushions) into the ventricular lumen are part of one continuous myocardial remodeling process, which involves the dissociation of the myocardial layers. For the endocardial cushions, this process results in delamination from the AV myocardium. The AV myocardium that will harbor the right lateral cushion is the exception and becomes positioned in the ventricular lumen by folding of the right ventricle. As a consequence, remodeling of the left and right AV myocardium occurs differently with implications for the formation of the mural leaflets and annulus fibrosis. At both the right and left side, the valvular myocardium harbors a distinct molecular phenotype and its removal from the cardiac leaflets involves a second wave of delamination. Interestingly, in the TGFβ2-KO mouse, which is a known model for cushion and valve defects, remodeling of the early myocardium is disturbed as indicated by defective trabeculae formation, persistence of valvular myocardium, disturbed myocardial phenotypes and differential defects at left and right side of the AV canal. Based on these results we propose a new model clarifying early trabeculae formation and AV valve formation and provide new inroads for an enhanced understanding of congenital heart defects.
MeSH term(s)	Animals ; Apoptosis ; Endocardium/embryology ; Heart/embryology ; Heart Atria/pathology ; Heart Valves/embryology ; Heart Valves/pathology ; Heart Ventricles/pathology ; Mice ; Mice, Knockout ; Myocardium/metabolism ; Myocardium/pathology ; T-Box Domain Proteins/metabolism ; Time Factors ; Transforming Growth Factor beta2/metabolism ; Ventricular Remodeling/physiology
Chemical Substances	T-Box Domain Proteins ; Tbx3 protein, mouse ; Tgfb2 protein, mouse ; Transforming Growth Factor beta2
Language	English
Publishing date	2013
Publishing country	Spain
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1036070-0
ISSN	1696-3547 ; 0214-6282
ISSN (online)	1696-3547
ISSN	0214-6282
DOI	10.1387/ijdb.130302bk
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3202: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Tempting fate: BMP signals for cardiac morphogenesis.

Schneider, Michael D / Gaussin, Vinciane / Lyons, Karen M

Cytokine & growth factor reviews

2002 Volume 14, Issue 1, Page(s) 1–4

Abstract: Heart muscle cell specification (cardiac myogenesis) and creating the four-chambered heart (cardiac morphogenesis) are subject to regulation, in certain model organisms, by bone morphogenetic proteins and their receptors. Extrapolation to mammals from ... ...

Abstract	Heart muscle cell specification (cardiac myogenesis) and creating the four-chambered heart (cardiac morphogenesis) are subject to regulation, in certain model organisms, by bone morphogenetic proteins and their receptors. Extrapolation to mammals from organisms that develop outside the mother (flies, fish, frogs, and avians) has been confounded by very early lethality-at gastrulation-of many null alleles needed to prove cause-effect relations in this pathway. Here, we describe the use of lineage- or compartment-restricted null alleles as well as hypomorphic alleles, which circumvent these limitations and pinpoint novel essential functions for the bone morphogenetic protein cascade in mammalian cardiac development.
MeSH term(s)	Animals ; Bone Morphogenetic Protein Receptors ; Bone Morphogenetic Proteins/genetics ; Bone Morphogenetic Proteins/metabolism ; Gene Targeting ; Heart/growth & development ; Morphogenesis ; Receptors, Growth Factor/genetics ; Receptors, Growth Factor/metabolism ; Signal Transduction/physiology
Chemical Substances	Bone Morphogenetic Proteins ; Receptors, Growth Factor ; Bone Morphogenetic Protein Receptors (EC 2.7.11.30)
Language	English
Publishing date	2002-12-16
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S. ; Review
ZDB-ID	1330534-7
ISSN	1359-6101
ISSN	1359-6101
DOI	10.1016/s1359-6101(02)00053-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2653: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Design of a Miniature Tissue Culture System to Culture Mouse Heart Valves

Lieber, Samuel C / Kruithof, Boudewijn P. T / Aubry, Nadine / Vatner, Stephen F / Gaussin, Vinciane

Annals of biomedical engineering. 2010 Mar., v. 38, no. 3

2010

Abstract	Valvular heart disease is a leading cause of morbidity and mortality in adults but little is known about the underlying etiology. A better understanding of the genetic and hemodynamic mechanisms involved in growth and remodeling of heart valves during physiological and pathological conditions is needed for a better understanding of valvular heart disease. Here, we report the design of a miniature tissue culture system (MTCS) that allows the culture of mitral valves from perinatal to adult mice. The design of the MTCS is novel in that fine positioning and cannulation can be conducted with hearts of different sizes (perinatal to adult). Perfusion of the heart and hence, culture of the mitral valve in its natural position, occurs in a hydraulically sealed culture bath environment. Using the MTCS, we successfully cultured the mitral valve of adult mouse hearts for 3 days. Histological analysis indicated that the cultured valves remained viable and their extracellular matrix organization was similar to age-matched native valves. Gene expression could also be modified in cultured valves by perfusion with medium containing beta-galactosidase-expressing adenovirus. Thus, the MTCS is a new tool to study the genetic and hemodynamic mechanisms underlying the three-dimensional organization of the heart valves, which could provide insights in the pathology of valvular heart disease and be used in animal models for the development of tissue-engineered heart valves.
Language	English
Dates of publication	2010-03
Size	p. 674-682.
Publisher	Springer US
Publishing place	Boston
Document type	Article
ZDB-ID	185984-5
ISSN	1573-9686 ; 0191-5649 ; 0090-6964
ISSN (online)	1573-9686
ISSN	0191-5649 ; 0090-6964
DOI	10.1007/s10439-010-9922-8
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 1018: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: An in vivo map of bone morphogenetic protein 2 post-transcriptional repression in the heart.

Kruithof, Boudewijn P T / Xu, Junwang / Fritz, David T / Cabral, Carolina S / Gaussin, Vinciane / Rogers, Melissa B

Genesis (New York, N.Y. : 2000)

2011 Volume 49, Issue 11, Page(s) 841–850

Abstract: The Bmp2 3'untranslated region (UTR) sequence bears a sequence conserved between mammals and fishes that can post-transcriptionally activate or repress protein synthesis. We developed a map of embryonic cells in the mouse where this potent Bmp2 ... ...

Abstract	The Bmp2 3'untranslated region (UTR) sequence bears a sequence conserved between mammals and fishes that can post-transcriptionally activate or repress protein synthesis. We developed a map of embryonic cells in the mouse where this potent Bmp2 regulatory sequence functions by using a lacZ reporter transgene with a 3'UTR bearing two loxP sites flanking the ultra-conserved sequence. Cre-recombinase-mediated deletion of the ultra-conserved sequence caused strong ectopic expression in proepicardium, epicardium and epicardium-derived cells (EPDC) and in tissues with known epicardial contributions (coronary vessels and valves). Transient transfections of reporters in the epicardial/mesothelial cell (EMC) line confirmed this repression. Ectopic expression of the recombined transgene also occurred in the aorta, outlet septum, posterior cardiac plexus, cardiac and extracardiac nerves and neural ganglia. Bmp2 is dynamically regulated in the developing heart. 3'UTR-mediated mechanisms that restrain BMP2 synthesis may be relevant to congenital heart and vasculature malformations and to adult diseases involving aberrant BMP2 synthesis.
MeSH term(s)	3' Untranslated Regions ; Animals ; Bone Morphogenetic Protein 2/genetics ; Bone Morphogenetic Protein 2/metabolism ; Cell Line ; Conserved Sequence ; Embryo, Mammalian/cytology ; Embryo, Mammalian/metabolism ; Embryo, Mammalian/physiology ; Embryonic Development ; Gene Expression Regulation, Developmental ; Genes, Reporter ; Heart/embryology ; Heart/innervation ; Heart/physiology ; Immunohistochemistry ; Integrases/metabolism ; Lac Operon ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic/genetics ; Mice, Transgenic/metabolism ; Neurofilament Proteins/genetics ; Neurofilament Proteins/metabolism ; Pericardium/cytology ; Pericardium/embryology ; Pericardium/metabolism ; Pericardium/physiology ; Protein Processing, Post-Translational ; Rats ; Regulatory Sequences, Nucleic Acid ; Sequence Deletion ; Transcription, Genetic ; Transfection ; Transgenes
Chemical Substances	3' Untranslated Regions ; Bmp2 protein, mouse ; Bone Morphogenetic Protein 2 ; Neurofilament Proteins ; neurofilament protein M (111365-29-8) ; Cre recombinase (EC 2.7.7.-) ; Integrases (EC 2.7.7.-)
Language	English
Publishing date	2011-10-14
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2004544-X
ISSN	1526-968X ; 1526-954X
ISSN (online)	1526-968X
ISSN	1526-954X
DOI	10.1002/dvg.20757
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2772: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito