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Article ; Online: Modulating pro-adhesive nature of metallic surfaces through a polypeptide coupling via diazonium chemistry.

Patel, Taral / Skonieczna, Magdalena / Turczyn, Roman / Krukiewicz, Katarzyna

2023 Volume 13, Issue 1, Page(s) 18365

Abstract: The design of biomaterials able to facilitate cell adhesion is critical in the field of tissue engineering. Precise control of surface chemistry at the material/tissue interface plays a major role in enhancing the interactions between a biomaterial and ... ...

Abstract	The design of biomaterials able to facilitate cell adhesion is critical in the field of tissue engineering. Precise control of surface chemistry at the material/tissue interface plays a major role in enhancing the interactions between a biomaterial and living cells. Bio-integration is particularly important in case of various electrotherapies, since a close contact between tissue and electrode's surface facilitates treatment. A promising approach towards surface biofunctionalization involves the electrografting of diazonium salts followed by the modification of organic layer with pro-adhesive polypeptides. This study focuses on the modification of platinum electrodes with a 4-nitrobenzenediazonium layer, which is then converted to the aminobenzene moiety. The electrodes are further biofunctionalized with polypeptides (polylysine and polylysine/laminin) to enhance cell adhesion. This study also explores the differences between physical and chemical coupling of selected polypeptides to modulate pro-adhesive nature of Pt electrodes with respect to human neuroblastoma SH-SY5Y cells and U87 astrocytes. Our results demonstrate the significant enhancement in cell adhesion for biofunctionalized electrodes, with more amplified adhesion noted for covalently coupled polypeptides. The implications of this research are crucial for the development of more effective and functional biomaterials, particularly biomedical electrodes, which have the potential to advance the field of bioelectronics and improve patients' outcomes.
MeSH term(s)	Humans ; Polylysine ; Adhesives ; Neuroblastoma ; Biocompatible Materials ; Peptides ; Cell Adhesion ; Surface Properties
Chemical Substances	Polylysine (25104-18-1) ; Adhesives ; Biocompatible Materials ; Peptides
Language	English
Publishing date	2023-10-26
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-45694-z
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Modulating pro-adhesive nature of metallic surfaces through a polypeptide coupling via diazonium chemistry

Taral Patel / Magdalena Skonieczna / Roman Turczyn / Katarzyna Krukiewicz

Scientific Reports, Vol 13, Iss 1, Pp 1-

2023 Volume 10

Abstract: Abstract The design of biomaterials able to facilitate cell adhesion is critical in the field of tissue engineering. Precise control of surface chemistry at the material/tissue interface plays a major role in enhancing the interactions between a ... ...

Abstract	Abstract The design of biomaterials able to facilitate cell adhesion is critical in the field of tissue engineering. Precise control of surface chemistry at the material/tissue interface plays a major role in enhancing the interactions between a biomaterial and living cells. Bio-integration is particularly important in case of various electrotherapies, since a close contact between tissue and electrode's surface facilitates treatment. A promising approach towards surface biofunctionalization involves the electrografting of diazonium salts followed by the modification of organic layer with pro-adhesive polypeptides. This study focuses on the modification of platinum electrodes with a 4-nitrobenzenediazonium layer, which is then converted to the aminobenzene moiety. The electrodes are further biofunctionalized with polypeptides (polylysine and polylysine/laminin) to enhance cell adhesion. This study also explores the differences between physical and chemical coupling of selected polypeptides to modulate pro-adhesive nature of Pt electrodes with respect to human neuroblastoma SH-SY5Y cells and U87 astrocytes. Our results demonstrate the significant enhancement in cell adhesion for biofunctionalized electrodes, with more amplified adhesion noted for covalently coupled polypeptides. The implications of this research are crucial for the development of more effective and functional biomaterials, particularly biomedical electrodes, which have the potential to advance the field of bioelectronics and improve patients' outcomes.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
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Article ; Online: Toxicity evaluation of choline ionic liquid-based nanocarriers of pharmaceutical agents for lung treatment.

Niesyto, Katarzyna / Skonieczna, Magdalena / Adamiec-Organiściok, Małgorzata / Neugebauer, Dorota

Journal of biomedical materials research. Part B, Applied biomaterials

2023 Volume 111, Issue 7, Page(s) 1374–1385

Abstract: In vitro cytotoxicity evaluation of linear copolymer (LC) containing choline ionic liquid units and its conjugates with an antibacterial drug in anionic form, that is, p-aminosalicylate (LC_PAS), clavulanate (LC_CLV), or piperacillin (LC_PIP) was carried ...

Abstract	In vitro cytotoxicity evaluation of linear copolymer (LC) containing choline ionic liquid units and its conjugates with an antibacterial drug in anionic form, that is, p-aminosalicylate (LC_PAS), clavulanate (LC_CLV), or piperacillin (LC_PIP) was carried out. These systems were tested against normal: human bronchial epithelial cells (BEAS-2B), and cancers: adenocarcinoma human alveolar basal epithelial cells (A549), and human non-small cell lung carcinoma cell line (H1299). Cells viability, after linear copolymer LC and their conjugates addition for 72 h, was measured at concentration range of 3.125-100 μg/mL. The MTT test allowed the designation of IC
MeSH term(s)	Humans ; Cell Line ; Choline/pharmacology ; Ionic Liquids/pharmacology ; Lung ; Antineoplastic Agents/pharmacology
Chemical Substances	Choline (N91BDP6H0X) ; Ionic Liquids ; Antineoplastic Agents
Language	English
Publishing date	2023-03-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2099992-6
ISSN	1552-4981 ; 1552-4973 ; 0021-9304
ISSN (online)	1552-4981
ISSN	1552-4973 ; 0021-9304
DOI	10.1002/jbm.b.35241
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Article ; Online: Polyurethane-Based Nanocomposites for Regenerative Therapies of Cancer Skin Surgery with Low Inflammatory Potential to Healthy Fibroblasts and Keratinocytes In Vitro

Maciej Mrówka / Joanna Lenża-Czempik / Anahit Dawicka / Magdalena Skonieczna

ACS Omega, Vol 8, Iss 41, Pp 37769-

2023 Volume 37780

Keywords	Chemistry ; QD1-999
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	American Chemical Society
Document type	Article ; Online
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Article: Compensative Resistance to Erastin-Induced Ferroptosis in GPX4 Knock-Out Mutants in HCT116 Cell Lines.

Adamiec-Organisciok, Malgorzata / Wegrzyn, Magdalena / Cienciala, Lukasz / Sojka, Damian / Nackiewicz, Joanna / Skonieczna, Magdalena

Pharmaceuticals (Basel, Switzerland)

2023 Volume 16, Issue 12

Abstract: Ferroptosis results from the accumulation of oxidized and damaged lipids which then leads to programmed cell death. This programmed process is iron-dependent, and as a fundamental biological process, plays a crucial role in tissue homeostasis. The ... ...

Abstract	Ferroptosis results from the accumulation of oxidized and damaged lipids which then leads to programmed cell death. This programmed process is iron-dependent, and as a fundamental biological process, plays a crucial role in tissue homeostasis. The ferroptosis molecular pathway depends on self-regulatory genes: GPX4; TFRC; ACSL4; FSP1; SLC7A11, and PROM2. Some of them were considered here as ferro-sensitive or ferro-resistance markers. We examined the impact of GPX4 gene knock-out, using the CRISPR/Cas-9 technique, on ferroptosis induction in the HCT116 colorectal cancer cell line. The results confirmed that cells lacking the GPX4 gene (GPX4 KO) should be more susceptible to ferroptosis after erastin treatment. However, the decrease in cell viability was not as significant as we initially assumed. Based on the lipid peroxidation markers profile and RT-qPCR gene expression analysis, we revealed the activation of an alternative antioxidant system supporting GPX4 KO cells, mostly for cellular ferroptotic death avoidance. Increased expression of FSP1 and PRDX1 genes in knock-out mutants was associated with their function-recognized here as ferroptosis suppressors. For such reasons, studies on the role of GPX4 and other crucial genes from the ferroptotic pathway should be explored. Despite promising prospects, the utilization of ferroptosis mechanisms in cancer therapy remains at the stage of experimental and in vitro preclinical studies.
Language	English
Publishing date	2023-12-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2193542-7
ISSN	1424-8247
ISSN	1424-8247
DOI	10.3390/ph16121710
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Article ; Online: Polyurethane-Based Nanocomposites for Regenerative Therapies of Cancer Skin Surgery with Low Inflammatory Potential to Healthy Fibroblasts and Keratinocytes In Vitro.

Mrówka, Maciej / Lenża-Czempik, Joanna / Dawicka, Anahit / Skonieczna, Magdalena

ACS omega

2023 Volume 8, Issue 41, Page(s) 37769–37780

Abstract: Nanocomposites based on thermoplastic polyurethanes (TPUs) filled with halloysite nanotubes (HNTs) were studied for their physicochemical and biological properties. Nanocomposites containing halloysite nanotube filler contents of 1 and 2% (E+1 and E+2), ... ...

Abstract	Nanocomposites based on thermoplastic polyurethanes (TPUs) filled with halloysite nanotubes (HNTs) were studied for their physicochemical and biological properties. Nanocomposites containing halloysite nanotube filler contents of 1 and 2% (E+1 and E+2), respectively, were obtained by extrusion. The newly formed E+1 and E+2 nanomaterials exhibited better flexibility and similar thermal properties compared to neat polyurethane. The use of atomic force microscopy (AFM) and differential scanning calorimetry (DSC) thermogram analysis showed that the distribution of halloysite nanotubes in the polymer matrix is more evenly dispersed in the E+1 nanomaterial, where the grains in the E+2 nanomaterial have a greater tendency to form agglomerates. Mechanical tests have shown that nanocomposites with the addition of HNT are characterized by a higher stress at break and elongation at break compared to neat TPU. The results of cytotoxicity tests suggest that the nanocomposite materials express lower toxicity to normal HaCaT and NHDF than to cancer Me45 cells. Further studies showed that the tested materials induced the expression of proinflammatory interleukins IL6 and IL8 in normal cells, but their overexpression in the cancer cell line resulted in cytostatic effects and proliferation reduction. Such a conclusion suggests the possible application of tested materials for regenerative therapies in cancer surgeries.
Language	English
Publishing date	2023-10-02
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.3c01663
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Article ; Online: Surface grafting of poly-L-lysine via diazonium chemistry to enhance cell adhesion to biomedical electrodes.

Patel, Taral / Skorupa, Małgorzata / Skonieczna, Magdalena / Turczyn, Roman / Krukiewicz, Katarzyna

Bioelectrochemistry (Amsterdam, Netherlands)

2023 Volume 152, Page(s) 108465

Abstract: The ability to study and regulate cell behavior at a biomaterial interface requires a strict control over its surface chemistry. Significance of studying cell adhesion in vitro and in vivo has become increasingly important, particularly in the field of ... ...

Abstract	The ability to study and regulate cell behavior at a biomaterial interface requires a strict control over its surface chemistry. Significance of studying cell adhesion in vitro and in vivo has become increasingly important, particularly in the field of tissue engineering and regenerative medicine. A promising surface modification route assumes using organic layers prepared by the method of electrografting of diazonium salts and their further functionalization with biologically active molecules as cell adhesion promoters. This work reports the modification of platinum electrodes with selected diazonium salts and poly-L-lysine to increase the number of sites available for cell adhesion. As-modified electrodes were characterized in terms of their chemical and morphological properties, as well as wettability. In order to monitor the process of cell attachment, biofunctionalized electrodes were used as substrates for culturing human neuroblastoma SH-SY5Y cells. The experiments revealed that cell adhesion is favored on the surface of diazonium-modified and poly-L-lysine coated electrodes, indicating proposed modification route as a valuable strategy enhancing the integration between bioelectronic devices and neural cells.
MeSH term(s)	Humans ; Cell Adhesion ; Polylysine ; Surface Properties ; Salts ; Neuroblastoma ; Electrodes
Chemical Substances	Polylysine (25104-18-1) ; Salts
Language	English
Publishing date	2023-05-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2010650-6
ISSN	1878-562X ; 0302-4598 ; 1567-5394
ISSN (online)	1878-562X
ISSN	0302-4598 ; 1567-5394
DOI	10.1016/j.bioelechem.2023.108465
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Article ; Online: Diversity among activated sludge in vacuum degassed laboratory systems.

Anna, Gnida / Magdalena, Skonieczna

Journal of environmental management

2021 Volume 281, Page(s) 111870

Abstract: Vacuum degassing of activated sludge is a technology used to improve sludge settling. By improving the settling ability of the sludge, a higher amount of biomass can be kept in the bioreactor, which further results in better wastewater treatment results. ...

Abstract	Vacuum degassing of activated sludge is a technology used to improve sludge settling. By improving the settling ability of the sludge, a higher amount of biomass can be kept in the bioreactor, which further results in better wastewater treatment results. However, the momentaneous vacuum exposition has been found a stress agent for activated sludge flocs and bacteria and may cause changes in sludge activity. However, no biological studies on the long-term intermittent application of vacuum to activated sludge have been published so far. The question arises whether the improvement in the degree of wastewater treatment results from an increase in the amount of biomass involved in the treatment process or does the change in pressure stimulate bacteria to increased activity? The study aimed to examine whether and how cyclic pressure reduction in the biological system affects the activity and composition of bacterial biocenosis of activated sludge. Three sequencing batch reactors were operated for almost three months. The work cycle of two of them included a vacuum degassing stage inserted between reaction and settling stage. Degassing was obtained with a pressure of 300 or 30 hPa. In addition to the wastewater quality analyzes, the microbial activity, number and variety of activated sludge bacteria and the characteristics of activated sludge flocs were determined. There were no significant differences between the reactors in the obtained effects of nutrient removal. All reactors showed organic compounds removal around 93%, and 40% and 58% of nitrogen and phosphorus removal, respectively. Obtained differences in respiratory and dehydrogenase activity were not significant. The biodiversity assessed with DNA sequencing revealed sludge enrichment with unclassified bacteria. Moreover, vacuum degassing caused flocs disintegration. In both the vacuum degassing reactors, the floc size range was much narrower than that of the control sludge. In the sludge degassed with a pressure of 30 hPa, the flocs were 25-80% smaller than in the sludge without the influence of a vacuum. The total number of bacteria was comparable among the reactors, however, in the reactor with degassing pressure of 30 hPa, the share of dead bacteria in the activated sludge (11%) was significantly lower than in other reactors (about 16%). The concentration of extracellular polymers in activated sludge was up to 87% higher when using vacuum degassing of 30 hPa than in other reactors. The results of the presented research show that the changes in the activated sludge occurring under the influence of vacuum degassing do not change the effectiveness of wastewater treatment, but may alter the community composition.
MeSH term(s)	Bioreactors ; Laboratories ; Sewage ; Vacuum ; Waste Disposal, Fluid
Chemical Substances	Sewage
Language	English
Publishing date	2021-01-09
Publishing country	England
Document type	Journal Article
ZDB-ID	184882-3
ISSN	1095-8630 ; 0301-4797
ISSN (online)	1095-8630
ISSN	0301-4797
DOI	10.1016/j.jenvman.2020.111870
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Article ; Online: Compensative Resistance to Erastin-Induced Ferroptosis in GPX4 Knock-Out Mutants in HCT116 Cell Lines

Malgorzata Adamiec-Organisciok / Magdalena Wegrzyn / Lukasz Cienciala / Damian Sojka / Joanna Nackiewicz / Magdalena Skonieczna

Pharmaceuticals, Vol 16, Iss 12, p

2023 Volume 1710

Abstract	Ferroptosis results from the accumulation of oxidized and damaged lipids which then leads to programmed cell death. This programmed process is iron-dependent, and as a fundamental biological process, plays a crucial role in tissue homeostasis. The ferroptosis molecular pathway depends on self-regulatory genes: GPX4; TFRC; ACSL4; FSP1; SLC7A11, and PROM2. Some of them were considered here as ferro-sensitive or ferro-resistance markers. We examined the impact of GPX4 gene knock-out, using the CRISPR/Cas-9 technique, on ferroptosis induction in the HCT116 colorectal cancer cell line. The results confirmed that cells lacking the GPX4 gene (GPX4 KO) should be more susceptible to ferroptosis after erastin treatment. However, the decrease in cell viability was not as significant as we initially assumed. Based on the lipid peroxidation markers profile and RT-qPCR gene expression analysis, we revealed the activation of an alternative antioxidant system supporting GPX4 KO cells, mostly for cellular ferroptotic death avoidance. Increased expression of FSP1 and PRDX1 genes in knock-out mutants was associated with their function—recognized here as ferroptosis suppressors. For such reasons, studies on the role of GPX4 and other crucial genes from the ferroptotic pathway should be explored. Despite promising prospects, the utilization of ferroptosis mechanisms in cancer therapy remains at the stage of experimental and in vitro preclinical studies.
Keywords	glutathione peroxidase GPX4 ; ferroptosis cell death ; ferroptosis-resistance ; ferroptosis-sensitiveness ; CRISPR/Cas-9 technique ; Medicine ; R ; Pharmacy and materia medica ; RS1-441
Subject code	616
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: UV radiation in HCT 116 cells influences intracellular H2O2 and glutathione levels, antioxidant expression, and protein glutathionylation.

Adamiec, Małgorzata / Skonieczna, Magdalena

Acta biochimica Polonica

2020 Volume 66, Issue 4, Page(s) 605–610

Abstract: UV radiation influences cellular levels of hydrogen peroxide (H2O2) and glutathione (GSH) and alters the expression of antioxidant genes in the human colorectal cancer cell line HCT116. In this study, cells were irradiated with UV light of different ... ...

Abstract	UV radiation influences cellular levels of hydrogen peroxide (H2O2) and glutathione (GSH) and alters the expression of antioxidant genes in the human colorectal cancer cell line HCT116. In this study, cells were irradiated with UV light of different wavelength (A, B, or C). A surge in H2O2 concentration and total glutathione (level occurred 6 hours later. Consequently, protein glutathionylation increased above control levels. Expression of the antioxidant enzymes: glutathione peroxidase (GPX) and glutathione reductase (GSR), assessed by real-time quantitative PCR, increased by 1.5-2 times after 24 hours post-irradiation, in comparison to the untreated controls. Glutathionylation of proteins was enhanced after UV radiation and the set of biotinylated glutathione ethyl ester (BioGEE) tagged proteins was detected by Western Blot procedure. This specific glutathione analogue is conjugated with antioxidant proteins during glutathionylation especially under oxidized conditions in cells. A pool of glutathionylated proteins in the treated cells showed peculiar characteristics. These proteins exhibited varying molecular weights. For UVA-irradiated cells, 24 hours after the treatment we observed two additional ~60 and ~72 kDa bands of glutathionylated proteins from NADPH oxidases (NOX family). Total glutathione level in the UV-irradiated HCT116 cells was higher than in the control. This correlates with the detection of glutathionylation in UV-irradiated cells in the first and twelfth hour of post-irradiation, and can be defined as a specific antioxidant element activation for cellular protection.
MeSH term(s)	Antioxidants/metabolism ; Colorectal Neoplasms/genetics ; Colorectal Neoplasms/metabolism ; Gene Expression Regulation, Neoplastic/radiation effects ; Glutathione/metabolism ; Glutathione Peroxidase/genetics ; Glutathione Reductase/genetics ; HCT116 Cells ; Humans ; Hydrogen Peroxide/metabolism ; NADPH Oxidases/genetics ; Oxidation-Reduction/drug effects ; Oxidative Stress/genetics ; Ultraviolet Rays
Chemical Substances	Antioxidants ; Hydrogen Peroxide (BBX060AN9V) ; Glutathione Peroxidase (EC 1.11.1.9) ; NADPH Oxidases (EC 1.6.3.-) ; Glutathione Reductase (EC 1.8.1.7) ; Glutathione (GAN16C9B8O)
Language	English
Publishing date	2020-01-13
Publishing country	Poland
Document type	Journal Article
ZDB-ID	595762-x
ISSN	1734-154X ; 0001-527X
ISSN (online)	1734-154X
ISSN	0001-527X
DOI	10.18388/abp.2019_2892
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