LIVIVO - Search results -

Search results

Result 1 - 10 of total 160

Search options

Article ; Online: Human Induced Pluripotent Stem Cell (iPSC) Handling Protocols: Maintenance, Expansion, and Cryopreservation.

Marotta, Davide / Rao, Chandrika / Fossati, Valentina

Methods in molecular biology (Clifton, N.J.)

2021 Volume 2454, Page(s) 1–15

Abstract: Human induced pluripotent stem cells (iPSCs) have emerged as an invaluable resource for basic research, disease modeling, and drug discovery over recent years. Given the numerous advantages of iPSCs over alternative models-including their human origin, ... ...

Abstract	Human induced pluripotent stem cells (iPSCs) have emerged as an invaluable resource for basic research, disease modeling, and drug discovery over recent years. Given the numerous advantages of iPSCs over alternative models-including their human origin, their ability to be differentiated into almost any cell type, and the therapeutic potential of patient-specific iPSCs in personalized medicine-many labs are now considering iPSC models for their studies. As the quality of the starting population of iPSCs is a key determinant in the success of any one of these applications, it is crucial to adhere to best practices in iPSC culture. In the following protocol, we offer a comprehensive guide to the culture, cryopreservation, and quality control methods required for the establishment and maintenance of high-quality iPSC cultures.
MeSH term(s)	Cell Differentiation ; Cryopreservation ; Humans ; Induced Pluripotent Stem Cells ; Precision Medicine
Language	English
Publishing date	2021-04-09
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/7651_2021_358
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article: Psychiatrists' attitudes towards functional neurological disorders: results from a national survey.

Marotta, Angela / Lasalvia, Antonio / Fiorio, Mirta / Zanalda, Enrico / Di Sciascio, Guido / Palumbo, Claudia / Papola, Davide / Barbui, Corrado / Tinazzi, Michele

Frontiers in psychiatry

2023 Volume 14, Page(s) 1216756

Abstract: Introduction: Functional neurological disorder (FND) presents motor, sensory, and cognitive symptoms characterized by clinical signs incongruent with known neurological disease. Together with other health professionals, like neurologists, psychiatrists ... ...

Abstract	Introduction: Functional neurological disorder (FND) presents motor, sensory, and cognitive symptoms characterized by clinical signs incongruent with known neurological disease. Together with other health professionals, like neurologists, psychiatrists can play an essential role in diagnosing and managing these disorders. Hence, understanding their opinion and clinical experience with FND is of utmost importance to catch potential educational needs and improve healthcare services for patients. This study aims at assessing the knowledge, opinion, and clinical approach of Italian psychiatrists to FND. Methods: Members of the Italian Society of Psychiatry completed a 14-item web-based survey investigating their approach to FND. Results. Overall, 174 questionnaires were completed. Our main findings suggest that Italian psychiatrists have a psychogenetic conceptualization of FND. "Conversion disorders", in fact, is the term most frequently used by Italian psychiatrists to refer to FND, thus implying a psychological etiology of these disorders. Congruently with this view, psychotherapy associated with pharmacological therapy is considered the most appropriate treatment by psychiatrists, while physiotherapy is an under-recognized treatment option for FND. Discussion: The present study highlights that a psychogenetic view of FND dominates among Italian psychiatrists. This could be due to out-of-date knowledge about the pathophysiology of this group of disorders. Promoting education about novel approaches to FND would be of crucial importance to improving care for patients suffering from this condition.
Language	English
Publishing date	2023-07-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2564218-2
ISSN	1664-0640
ISSN	1664-0640
DOI	10.3389/fpsyt.2023.1216756
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Psychiatrists’ attitudes towards functional neurological disorders

Angela Marotta / Antonio Lasalvia / Mirta Fiorio / Enrico Zanalda / Guido Di Sciascio / Claudia Palumbo / Davide Papola / Corrado Barbui / Michele Tinazzi

Frontiers in Psychiatry, Vol

results from a national survey

2023 Volume 14

Abstract: IntroductionFunctional neurological disorder (FND) presents motor, sensory, and cognitive symptoms characterized by clinical signs incongruent with known neurological disease. Together with other health professionals, like neurologists, psychiatrists can ...

Abstract	IntroductionFunctional neurological disorder (FND) presents motor, sensory, and cognitive symptoms characterized by clinical signs incongruent with known neurological disease. Together with other health professionals, like neurologists, psychiatrists can play an essential role in diagnosing and managing these disorders. Hence, understanding their opinion and clinical experience with FND is of utmost importance to catch potential educational needs and improve healthcare services for patients. This study aims at assessing the knowledge, opinion, and clinical approach of Italian psychiatrists to FND.MethodsMembers of the Italian Society of Psychiatry completed a 14-item web-based survey investigating their approach to FND. Results. Overall, 174 questionnaires were completed. Our main findings suggest that Italian psychiatrists have a psychogenetic conceptualization of FND. “Conversion disorders”, in fact, is the term most frequently used by Italian psychiatrists to refer to FND, thus implying a psychological etiology of these disorders. Congruently with this view, psychotherapy associated with pharmacological therapy is considered the most appropriate treatment by psychiatrists, while physiotherapy is an under-recognized treatment option for FND.DiscussionThe present study highlights that a psychogenetic view of FND dominates among Italian psychiatrists. This could be due to out-of-date knowledge about the pathophysiology of this group of disorders. Promoting education about novel approaches to FND would be of crucial importance to improving care for patients suffering from this condition.
Keywords	functional neurological disorders ; conversion disorders ; psychosomatic medicine ; psychiatric practice ; education ; survey ; Psychiatry ; RC435-571
Subject code	150
Language	English
Publishing date	2023-07-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Resting-State fMRI in Chronic Patients with Disorders of Consciousness: The Role of Lower-Order Networks for Clinical Assessment.

Medina, Jean Paul / Nigri, Anna / Stanziano, Mario / D'Incerti, Ludovico / Sattin, Davide / Ferraro, Stefania / Rossi Sebastiano, Davide / Pinardi, Chiara / Marotta, Giorgio / Leonardi, Matilde / Bruzzone, Maria Grazia / Rosazza, Cristina

Brain sciences

2022 Volume 12, Issue 3

Abstract: Resting-state fMRI (rs-fMRI) is a widely used technique to investigate the residual brain functions of patients with Disorders of Consciousness (DoC). Nonetheless, it is unclear how the networks that are more associated with primary functions, such as ... ...

Abstract	Resting-state fMRI (rs-fMRI) is a widely used technique to investigate the residual brain functions of patients with Disorders of Consciousness (DoC). Nonetheless, it is unclear how the networks that are more associated with primary functions, such as the sensory-motor, medial/lateral visual and auditory networks, contribute to clinical assessment. In this study, we examined the rs-fMRI lower-order networks alongside their structural MRI data to clarify the corresponding association with clinical assessment. We studied 109 chronic patients with DoC and emerged from DoC with structural MRI and rs-fMRI: 65 in vegetative state/unresponsive wakefulness state (VS/UWS), 34 in minimally conscious state (MCS) and 10 with severe disability. rs-fMRI data were analyzed with independent component analyses and seed-based analyses, in relation to structural MRI and clinical data. The results showed that VS/UWS had fewer networks than MCS patients and the rs-fMRI activity in each network was decreased. Visual networks were correlated to the clinical status, and in cases where no clinical response occurred, rs-fMRI indicated distinctive networks conveying information in a similar way to other techniques. The information provided by single networks was limited, whereas the four networks together yielded better classification results, particularly when the model included rs-fMRI and structural MRI data (AUC = 0.80). Both quantitative and qualitative rs-fMRI analyses yielded converging results; vascular etiology might confound the results, and disease duration generally reduced the number of networks observed. The lower-order rs-fMRI networks could be used clinically to support and corroborate visual function assessments in DoC.
Language	English
Publishing date	2022-03-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2651993-8
ISSN	2076-3425
ISSN	2076-3425
DOI	10.3390/brainsci12030355
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: NCLs and ER: A stressful relationship.

Marotta, Davide / Tinelli, Elisa / Mole, Sara E

Biochimica et biophysica acta. Molecular basis of disease

2017 Volume 1863, Issue 6, Page(s) 1273–1281

Abstract: The Neuronal Ceroid Lipofuscinoses (NCLs, Batten disease) are a group of inherited neurodegenerative disorders with variable age of onset, characterized by the lysosomal accumulation of autofluorescent ceroid lipopigments. The endoplasmic reticulum (ER) ... ...

Abstract	The Neuronal Ceroid Lipofuscinoses (NCLs, Batten disease) are a group of inherited neurodegenerative disorders with variable age of onset, characterized by the lysosomal accumulation of autofluorescent ceroid lipopigments. The endoplasmic reticulum (ER) is a critical organelle for normal cell function. Alteration of ER homeostasis leads to accumulation of misfolded protein in the ER and to activation of the unfolded protein response. ER stress and the UPR have recently been linked to the NCLs. In this review, we will discuss the evidence for UPR activation in the NCLs, and address its connection to disease pathogenesis. Further understanding of ER-stress response involvement in the NCLs may encourage development of novel therapeutical agents targeting these pathogenic pathways.
MeSH term(s)	Animals ; Endoplasmic Reticulum/genetics ; Endoplasmic Reticulum/metabolism ; Endoplasmic Reticulum/pathology ; Endoplasmic Reticulum Stress ; Humans ; Neuronal Ceroid-Lipofuscinoses/genetics ; Neuronal Ceroid-Lipofuscinoses/metabolism ; Neuronal Ceroid-Lipofuscinoses/pathology ; Neuronal Ceroid-Lipofuscinoses/therapy ; Unfolded Protein Response
Language	English
Publishing date	2017-04-06
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	60-7
ISSN	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0925-4439 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 1874-9399
ISSN (online)	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
ISSN	0925-4439 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 1874-9399
DOI	10.1016/j.bbadis.2017.04.003
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uc I Zs.247: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Impact of SARS-CoV-2 Epidemic on Antimicrobial Resistance: A Literature Review.

Segala, Francesco Vladimiro / Bavaro, Davide Fiore / Di Gennaro, Francesco / Salvati, Federica / Marotta, Claudia / Saracino, Annalisa / Murri, Rita / Fantoni, Massimo

Viruses

2021 Volume 13, Issue 11

Abstract: Antimicrobial resistance is an urgent threat to public health and global development; in this scenario, the SARS-CoV2 pandemic has caused a major disruption of healthcare systems and practices. A narrative review was conducted on articles focusing on the ...

Abstract	Antimicrobial resistance is an urgent threat to public health and global development; in this scenario, the SARS-CoV2 pandemic has caused a major disruption of healthcare systems and practices. A narrative review was conducted on articles focusing on the impact of COVID-19 on multidrug-resistant gram-negative, gram-positive bacteria, and fungi. We found that, worldwide, multiple studies reported an unexpected high incidence of infections due to methicillin-resistant
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Bacteria/drug effects ; Bacterial Infections/complications ; Bacterial Infections/epidemiology ; Bacterial Infections/microbiology ; COVID-19/complications ; COVID-19/epidemiology ; Coinfection/epidemiology ; Coinfection/microbiology ; Drug Resistance, Bacterial ; Drug Resistance, Fungal ; Drug Resistance, Multiple, Bacterial ; Fungi/drug effects ; Humans ; Infection Control ; Intensive Care Units ; Mycoses/complications ; Mycoses/epidemiology ; Mycoses/microbiology
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2021-10-20
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v13112110
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Antibody-independent protection against heterologous SARS-CoV-2 challenge conferred by prior infection or vaccination.

Fumagalli, Valeria / Ravà, Micol / Marotta, Davide / Di Lucia, Pietro / Bono, Elisa B / Giustini, Leonardo / De Leo, Federica / Casalgrandi, Maura / Monteleone, Emanuele / Mouro, Violette / Malpighi, Chiara / Perucchini, Chiara / Grillo, Marta / De Palma, Sara / Donnici, Lorena / Marchese, Silvia / Conti, Matteo / Muramatsu, Hiromi / Perlman, Stanley /

Pardi, Norbert / Kuka, Mirela / De Francesco, Raffaele / Bianchi, Marco E / Guidotti, Luca G / Iannacone, Matteo

Nature immunology

2024 Volume 25, Issue 4, Page(s) 633–643

Abstract: Vaccines have reduced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) morbidity and mortality, yet emerging variants challenge their effectiveness. The prevailing approach to updating vaccines targets the antibody response, operating under ... ...

Abstract	Vaccines have reduced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) morbidity and mortality, yet emerging variants challenge their effectiveness. The prevailing approach to updating vaccines targets the antibody response, operating under the presumption that it is the primary defense mechanism following vaccination or infection. This perspective, however, can overlook the role of T cells, particularly when antibody levels are low or absent. Here we show, through studies in mouse models lacking antibodies but maintaining functional B cells and lymphoid organs, that immunity conferred by prior infection or mRNA vaccination can protect against SARS-CoV-2 challenge independently of antibodies. Our findings, using three distinct models inclusive of a novel human/mouse ACE2 hybrid, highlight that CD8
MeSH term(s)	Humans ; Animals ; Mice ; SARS-CoV-2 ; CD8-Positive T-Lymphocytes ; COVID-19/prevention & control ; Antibodies ; Vaccination ; Vaccines ; Antibodies, Viral ; Antibodies, Neutralizing
Chemical Substances	Antibodies ; Vaccines ; Antibodies, Viral ; Antibodies, Neutralizing
Language	English
Publishing date	2024-03-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	2016987-5
ISSN	1529-2916 ; 1529-2908
ISSN (online)	1529-2916
ISSN	1529-2908
DOI	10.1038/s41590-024-01787-z
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5294: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 42: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Corrigendum: Preservation of Language Processing and Auditory Performance in Patients With Disorders of Consciousness: A Multimodal Assessment.

Ferraro, Stefania / Nigri, Anna / D'Incerti, Ludovico / Rosazza, Cristina / Sattin, Davide / Rossi Sebastiano, Davide / Visani, Elisa / Duran, Dunja / Marotta, Giorgio / Demichelis, Greta / Catricala', Eleonora / Kotz, Sonja / Verga, Laura / Leonardi, Matilde / Cappa, Stefano / Bruzzone, Maria Grazia

Frontiers in neurology

2021 Volume 12, Page(s) 656747

Abstract: This corrects the article DOI: 10.3389/fneur.2020.526465.]. ...

Abstract	[This corrects the article DOI: 10.3389/fneur.2020.526465.].
Language	English
Publishing date	2021-02-16
Publishing country	Switzerland
Document type	Published Erratum
ZDB-ID	2564214-5
ISSN	1664-2295
ISSN	1664-2295
DOI	10.3389/fneur.2021.656747
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Microglia reactivity entails microtubule remodeling from acentrosomal to centrosomal arrays.

Rosito, Maria / Sanchini, Caterina / Gosti, Giorgio / Moreno, Manuela / De Panfilis, Simone / Giubettini, Maria / Debellis, Doriana / Catalano, Federico / Peruzzi, Giovanna / Marotta, Roberto / Indrieri, Alessia / De Leonibus, Elvira / De Stefano, Maria Egle / Ragozzino, Davide / Ruocco, Giancarlo / Di Angelantonio, Silvia / Bartolini, Francesca

Cell reports

2023 Volume 42, Issue 2, Page(s) 112104

Abstract: Microglia reactivity entails a large-scale remodeling of cellular geometry, but the behavior of the microtubule cytoskeleton during these changes remains unexplored. Here we show that activated microglia provide an example of microtubule reorganization ... ...

Abstract	Microglia reactivity entails a large-scale remodeling of cellular geometry, but the behavior of the microtubule cytoskeleton during these changes remains unexplored. Here we show that activated microglia provide an example of microtubule reorganization from a non-centrosomal array of parallel and stable microtubules to a radial array of more dynamic microtubules. While in the homeostatic state, microglia nucleate microtubules at Golgi outposts, and activating signaling induces recruitment of nucleating material nearby the centrosome, a process inhibited by microtubule stabilization. Our results demonstrate that a hallmark of microglia reactivity is a striking remodeling of the microtubule cytoskeleton and suggest that while pericentrosomal microtubule nucleation may serve as a distinct marker of microglia activation, inhibition of microtubule dynamics may provide a different strategy to reduce microglia reactivity in inflammatory disease.
MeSH term(s)	Microglia ; Microtubules ; Centrosome ; Cytoskeleton ; Golgi Apparatus ; Tubulin
Chemical Substances	Tubulin
Language	English
Publishing date	2023-02-13
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2649101-1
ISSN	2211-1247 ; 2211-1247
ISSN (online)	2211-1247
ISSN	2211-1247
DOI	10.1016/j.celrep.2023.112104
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Does access to care play a role in liver cancer survival? The ten-year (2006-2015) experience from a population-based cancer registry in Southern Italy.

Mazzucco, Walter / Vitale, Francesco / Mazzola, Sergio / Amodio, Rosalba / Zarcone, Maurizio / Alba, Davide / Marotta, Claudia / Cusimano, Rosanna / Allemani, Claudia

BMC cancer

2021 Volume 21, Issue 1, Page(s) 307

Abstract: Background: Hepatocellular carcinoma (HCC) is the most frequent primary invasive cancer of the liver. During the last decade, the epidemiology of HCC has been continuously changing in developed countries, due to more effective primary prevention and to ... ...

Abstract	Background: Hepatocellular carcinoma (HCC) is the most frequent primary invasive cancer of the liver. During the last decade, the epidemiology of HCC has been continuously changing in developed countries, due to more effective primary prevention and to successful treatment of virus-related liver diseases. The study aims to examine survival by level of access to care in patients with HCC, for all patients combined and by age. Methods: We included 2018 adult patients (15-99 years) diagnosed with a primary liver tumour, registered in the Palermo Province Cancer Registry during 2006-2015, and followed-up to 30 October 2019. We obtained a proxy measure of access to care by linking each record to the Hospital Discharge Records and the Ambulatory Discharge Records. We estimated net survival up to 5 years after diagnosis by access to care ("easy access to care" versus "poor access to care"), using the Pohar-Perme estimator. Estimates were age-standardised using International Cancer Survival Standard (ICSS) weights. We also examined survival by access to care and age (15-64, 65-74 and ≥ 75 years). Results: Among the 2018 patients, 62.4% were morphologically verified and 37.6% clinically diagnosed. Morphologically verified tumours were more frequent in patients aged 65-74 years (41.6%), while tumours diagnosed clinically were more frequent in patients aged 75 years or over (50.2%). During 2006-2015, age-standardised net survival was higher among HCC patients with "easy access to care" than in those with "poor access to care" (68% vs. 48% at 1 year, 29% vs. 11% at 5 years; p < 0.0001). Net survival up to 5 years was higher for patients with "easy access to care" in each age group (p < 0.0001). Moreover, survival increased slightly for patients with easier access to care, while it remained relatively stable for patients with poor access to care. Conclusions: During 2006-2015, 5-year survival was higher for HCC patients with easier access to care, probably reflecting progressive improvement in the effectiveness of health care services offered to these patients. Our linkage algorithm could provide valuable evidence to support healthcare decision-making in the context of the evolving epidemiology of hepatocellular carcinoma.
MeSH term(s)	Adolescent ; Adult ; Age Distribution ; Aged ; Aged, 80 and over ; Carcinoma, Hepatocellular/mortality ; Carcinoma, Hepatocellular/therapy ; Decision Making, Organizational ; Decision Support Techniques ; Female ; Follow-Up Studies ; Health Services Accessibility/organization & administration ; Health Services Accessibility/statistics & numerical data ; Health Services Needs and Demand/statistics & numerical data ; Humans ; Italy/epidemiology ; Liver Neoplasms/mortality ; Liver Neoplasms/therapy ; Male ; Middle Aged ; Registries/statistics & numerical data ; Survival Analysis ; Young Adult
Language	English
Publishing date	2021-03-24
Publishing country	England
Document type	Journal Article
ISSN	1471-2407
ISSN (online)	1471-2407
DOI	10.1186/s12885-021-07935-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED