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  1. Article: Novel BRET combination for detection of rapamycin-induced protein dimerization using luciferase from fungus

    Mujawar, Aaiyas / Dimri, Shalini / Palkina, Ksenia A / Markina, Nadezhda M / Sarkisyan, Karen S / Balakireva, Anastasia V / Yampolsky, Ilia V / De, Abhijit

    Heliyon

    2024  Volume 10, Issue 4, Page(s) e25553

    Abstract: Bioluminescence resonance energy transfer (BRET) is one of the most promising approaches used for noninvasive imaging of protein-protein ... ...

    Abstract Bioluminescence resonance energy transfer (BRET) is one of the most promising approaches used for noninvasive imaging of protein-protein interactions
    Language English
    Publishing date 2024-02-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2024.e25553
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  2. Article ; Online: Direct knockdown of phospho-PTM targets mediated by TRIM21 can improve personalized treatment in breast cancer.

    Dey, Pranay / Joshi, Mansi / Mujawar, Aaiyas / Malhotra, Renu / De, Abhijit

    Cellular oncology (Dordrecht)

    2022  Volume 45, Issue 5, Page(s) 873–891

    Abstract: Purpose: In this work for the first time, we showed specific and direct knockdown of important oncogenic proteins of interest and their phospho-PTM targets in tripartite motif containing-21 (TRIM21) overexpressing breast cancer (BC) cells. We revealed ... ...

    Abstract Purpose: In this work for the first time, we showed specific and direct knockdown of important oncogenic proteins of interest and their phospho-PTM targets in tripartite motif containing-21 (TRIM21) overexpressing breast cancer (BC) cells. We revealed the functional and therapeutic consequences of this protein knockdown approach called 'TRIM-ing'.
    Methods: To target HER2, HER3, STAT3 or their activated forms, electroporation and puls-in transfection were standardized for mAb delivery in AU565 and MCF7 BC cell lines. Cancer cells were treated with HER2-targeted medicines (Trastuzumab and Neratinib) or STAT3 targeted inhibitors (Stattic and Niclosamide) with or without respective target TRIM-ing. Real-time PCR, immunoblotting, immunofluorescence, cytotoxicity, short- and long-term cell survival assessments were done following standard methodologies. 3-D structure modelling was used to verify the binding of mAb onto the STAT3 target.
    Results: TRIM-ing of HER2 or HER3 receptors or their activated phospho-forms in BC cells showed rapid degradation of respective protein forms, shattering down the downstream signaling (p-ERK, p-AKT) that lasts for up to 7-8 days. This significantly inhibited BC survival (p < 0.001), showing a synergistic therapeutic effect with HER2 medicine trastuzumab or neratinib. Additionally, specific TRIM-ing ability of canonical pY705 or non-canonical pS727 PTMs of STAT3 protein was demonstrated in MCF7 cells, causing significant cytotoxicity (p < 0.05). TRIM-ing of STAT3 PTM, when combined with the same PTM-specific inhibitors, a synergistic treatment effect was observed.
    Conclusion: The work demonstrated that TRIM-ing could directly reduce various oncogenic targets or their specific activated form inside the cancer cells without compensatory pathway activation, a conundrum limiting the therapeutic benefit of current personalized medicines.
    MeSH term(s) Humans ; Female ; Breast Neoplasms/drug therapy ; Breast Neoplasms/genetics ; Breast Neoplasms/metabolism ; Receptor, ErbB-2/metabolism ; STAT3 Transcription Factor/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Niclosamide/therapeutic use ; Precision Medicine ; Drug Resistance, Neoplasm ; Trastuzumab/pharmacology ; Trastuzumab/therapeutic use ; Cell Line, Tumor
    Chemical Substances Receptor, ErbB-2 (EC 2.7.10.1) ; STAT3 Transcription Factor ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Niclosamide (8KK8CQ2K8G) ; Trastuzumab (P188ANX8CK)
    Language English
    Publishing date 2022-07-14
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2595109-9
    ISSN 2211-3436 ; 1875-8606 ; 2211-3428
    ISSN (online) 2211-3436
    ISSN 1875-8606 ; 2211-3428
    DOI 10.1007/s13402-022-00693-6
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6908: Show issues Location:
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  3. Article: A retrospective three-dimensional assessment of the prevalence of apical periodontitis and quality of root canal treatment in Mid-West Indian population.

    Mujawar, Asiya / Hegde, Vivek / Srilatha, S

    Journal of conservative dentistry : JCD

    2021  Volume 24, Issue 2, Page(s) 184–189

    Abstract: ... presence of AP in the nonfilled teeth than filled ones (X2 - Chi square value M = 612.156, : Conclusion ...

    Abstract Introduction: This study aims to report a retrospective observation of the prevalence of apical periodontitis (AP) and quality of root canal treatment in cone-beam computed tomography (CBCT) scans among the Mid-West Indian population.
    Materials and methods: A total of 1229 CBCT scans were obtained across different CBCT centers in western India. After the exclusion criteria, those that were included were divided into broadly two groups - those that were filled and those that were unfilled. Various parameters were taken into account such as AP, length of the root canal filled, and coronal filling.
    Results: There were a significantly higher percentage of nonfilled canals. Poor filling quality, inadequate coronal restoration, and also missed extra canal were significantly associated with AP. Both males and females showed higher presence of AP in the nonfilled teeth than filled ones (X2 - Chi square value M = 612.156,
    Conclusion: (1) The inadequacy of the filling of the root length was a contributory factor to the higher prevalence of AP among the study population. (2) The density of the filling and the quality of the coronal filling also significantly affected the successful outcome of the root canal treatment.
    Language English
    Publishing date 2021-10-09
    Publishing country India
    Document type Journal Article
    ZDB-ID 2064481-4
    ISSN 0972-0707
    ISSN 0972-0707
    DOI 10.4103/jcd.jcd_44_21
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5545: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Point of Care Sensing Devices: Better Care for Everyone.

    Kaushik, Ajeet / Mujawar, Mubarak A

    Sensors (Basel, Switzerland)

    2018  Volume 18, Issue 12

    Abstract: Improved health management is a key to provide a better health care [ ... ]. ...

    Abstract Improved health management is a key to provide a better health care [...].
    MeSH term(s) Biosensing Techniques/instrumentation ; Electrochemical Techniques ; Humans ; Hydrocortisone/analysis ; Point-of-Care Systems
    Chemical Substances Hydrocortisone (WI4X0X7BPJ)
    Language English
    Publishing date 2018-12-06
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s18124303
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  5. Article ; Online: Effects of cold atmospheric plasma treatment on the morphological and optical properties of plasmonic silver nanoparticles.

    Sonawane, Apurva / Mujawar, Mubarak A / Bhansali, Shekhar

    Nanotechnology

    2020  Volume 31, Issue 36, Page(s) 365706

    Abstract: The use of plasma processes in nanomaterial synthesis is limited by a lack of understanding of the effects of plasma treatment on the morphology and other properties. Here, we studied the effects of atmospheric plasma treatment on the morphology and ... ...

    Abstract The use of plasma processes in nanomaterial synthesis is limited by a lack of understanding of the effects of plasma treatment on the morphology and other properties. Here, we studied the effects of atmospheric plasma treatment on the morphology and optical properties of Ag nanoparticles. The Ag nanoparticles were deposited on substrates by injecting an aerosol into flowing argon gas and then treated with a low-temperature atmospheric plasma jet. After plasma treatment, the mean Ag nanoparticle diameter reduced to an average of 5 nm, which was accompanied by a blue shift of ∼70 nm in the peak of the surface plasmon resonance; these results are similar to those obtained by thermal treatment at elevated temperatures. The reduction in nanoparticle size is explained by the redox reaction that occurs on the nanoparticle surface, which is evident from the presence of AgO and Ag
    Language English
    Publishing date 2020-05-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 1362365-5
    ISSN 1361-6528 ; 0957-4484
    ISSN (online) 1361-6528
    ISSN 0957-4484
    DOI 10.1088/1361-6528/ab9788
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  6. Article ; Online: Cellulose microcrystalline: A promising ecofriendly approach to control Culex quinquefasciatus larvae.

    Souza, Sindoval Silva de / Gomes, Alex Rodrigues / Guimarães, Abraão Tiago Batista / Rodrigues, Aline Sueli de Lima / de Matos, Letícia Paiva / Dos Santos Mendonça, Juliana / da Luz, Thiarlen Marinho / Matos, Stênio Gonçalves da Silva / Rahman, M Safiur / Ragavendran, Chinnasamy / Senthil-Nathan, Sengottayan / Guru, Ajay / Rakib, Md Refat Jahan / Mubarak, Nabisab Mujawar / Rahman, Md Mostafizur / Rocha, Thiago Lopes / Islam, Abu Reza Md Towfiqul / Malafaia, Guilherme

    The Science of the total environment

    2023  Volume 901, Page(s) 165952

    Abstract: The growing use of synthetic chemical compounds/substances in vector control of mosquitoes, associated with their adverse effects on the environment and non-target organisms, has demanded the development of eco-friendly alternatives. In this context, ... ...

    Abstract The growing use of synthetic chemical compounds/substances in vector control of mosquitoes, associated with their adverse effects on the environment and non-target organisms, has demanded the development of eco-friendly alternatives. In this context, this study aimed to evaluate the larvicidal action of different cellulose microcrystalline (CMs) concentrations and investigate their toxicity mechanisms in Culex quinquefasciatus fourth instar larvae as a model species. Probit analysis revealed that the median lethal concentrations (LC
    Language English
    Publishing date 2023-08-01
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2023.165952
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 949: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: Structural studies on 10-hydroxygeraniol dehydrogenase: A novel linear substrate-specific dehydrogenase from Catharanthus roseus.

    Sandholu, Anand S / Mujawar, Sharmila P / Ramakrishnan, Krithika / Thulasiram, Hirekodathakallu V / Kulkarni, Kiran

    Proteins

    2020  Volume 88, Issue 9, Page(s) 1197–1206

    Abstract: Conversion of 10-hydroxygeraniol to 10-oxogeranial is a crucial step in iridoid biosynthesis. This reaction is catalyzed by a zinc-dependent alcohol dehydrogenase, 10-hydroxygeraniol dehydrogenase, belonging to the family of medium-chain dehydrogenase/ ... ...

    Abstract Conversion of 10-hydroxygeraniol to 10-oxogeranial is a crucial step in iridoid biosynthesis. This reaction is catalyzed by a zinc-dependent alcohol dehydrogenase, 10-hydroxygeraniol dehydrogenase, belonging to the family of medium-chain dehydrogenase/reductase (MDR). Here, we report the crystal structures of a novel 10-hydroxygeraniol dehydrogenase from Catharanthus roseus in its apo and nicotinamide adenine dinucleotide phosphate (NADP
    MeSH term(s) Alcohol Dehydrogenase/chemistry ; Alcohol Dehydrogenase/genetics ; Alcohol Dehydrogenase/metabolism ; Amino Acid Sequence ; Apoproteins/chemistry ; Apoproteins/genetics ; Apoproteins/metabolism ; Binding Sites ; Catharanthus/chemistry ; Catharanthus/enzymology ; Cloning, Molecular ; Crystallography, X-Ray ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Gene Expression ; Genetic Vectors/chemistry ; Genetic Vectors/metabolism ; Zingiber officinale/chemistry ; Zingiber officinale/enzymology ; Kinetics ; Molecular Docking Simulation ; NADP/chemistry ; NADP/metabolism ; Plant Proteins/chemistry ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Protein Binding ; Protein Conformation, alpha-Helical ; Protein Conformation, beta-Strand ; Protein Interaction Domains and Motifs ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Sequence Alignment ; Structural Homology, Protein ; Substrate Specificity ; Terpenes/chemistry ; Terpenes/metabolism
    Chemical Substances Apoproteins ; Plant Proteins ; Recombinant Proteins ; Terpenes ; NADP (53-59-8) ; Alcohol Dehydrogenase (EC 1.1.1.1)
    Language English
    Publishing date 2020-03-26
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 806683-8
    ISSN 1097-0134 ; 0887-3585
    ISSN (online) 1097-0134
    ISSN 0887-3585
    DOI 10.1002/prot.25891
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2291: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  8. Article ; Online: Nano-enabled biosensing systems for intelligent healthcare: towards COVID-19 management.

    Mujawar, M A / Gohel, H / Bhardwaj, S K / Srinivasan, S / Hickman, N / Kaushik, A

    Materials today. Chemistry

    2020  Volume 17, Page(s) 100306

    Abstract: Biosensors are emerging as efficient (sensitive and selective) and affordable analytical diagnostic tools for early-stage disease detection, as required for personalized health wellness management. Low-level detection of a targeted disease biomarker (pM ... ...

    Abstract Biosensors are emerging as efficient (sensitive and selective) and affordable analytical diagnostic tools for early-stage disease detection, as required for personalized health wellness management. Low-level detection of a targeted disease biomarker (pM level) has emerged extremely useful to evaluate the progression of disease under therapy. Such collected bioinformatics and its multi-aspects-oriented analytics is in demand to explore the effectiveness of a prescribed treatment, optimize therapy, and correlate biomarker level with disease pathogenesis. Owing to nanotechnology-enabled advancements in sensing unit fabrication, device integration, interfacing, packaging, and sensing performance at point-of-care (POC) has rendered diagnostics according to the requirements of disease management and patient disease profile i.e. in a personalized manner. Efforts are continuously being made to promote the state of art biosensing technology as a next-generation non-invasive disease diagnostics methodology. Keeping this in view, this progressive opinion article describes personalized health care management related analytical tools which can provide access to better health for everyone, with overreaching aim to manage healthy tomorrow timely. Considering accomplishments and predictions, such affordable intelligent diagnostics tools are urgently required to manage COVID-19 pandemic, a life-threatening respiratory infectious disease, where a rapid, selective and sensitive detection of human beta severe acute respiratory system coronavirus (SARS-COoV-2) protein is the key factor.
    Keywords covid19
    Language English
    Publishing date 2020-06-05
    Publishing country England
    Document type Journal Article ; Review
    ISSN 2468-5194
    ISSN (online) 2468-5194
    DOI 10.1016/j.mtchem.2020.100306
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  9. Article ; Online: Bio-inspired graphene-based nano-systems for biomedical applications.

    Bharadwaj, Sheetal Kaushik / Mujawar, Mubarak / Mishra, Yogendra Kumar / Hickman, Nicoleta / Chavali, M / Kaushik, Ajeet

    Nanotechnology

    2021  

    Abstract: The increasing demands of environmentally sustainable, affordable, and scalable materials have inspired researchers to explore greener nanosystems of unique properties which can enhance the performance of existing systems. Such nanosystems, extracted ... ...

    Abstract The increasing demands of environmentally sustainable, affordable, and scalable materials have inspired researchers to explore greener nanosystems of unique properties which can enhance the performance of existing systems. Such nanosystems, extracted from nature, are state-of-art high-performance nanostructures due to intrinsic hierarchical micro/nanoscale architecture and generous interfacial interactions in natural resources. Among several, bio-inspired graphene nanosystems have emerged as an essential nano-platform wherein a highly electroactive, scalable, functional, flexible, and adaptable to a living being is a key factor. Preliminary investigation project bio-inspired graphene nanosystems as a multi-functional nano-platform suitable for electronic devices, energy storage, sensors, and medical sciences application. However, a broad understanding of bio-inspired graphene nanosystems and their projection towards applied application is not well-explored yet. Considering this as a motivation, this mini-review highlights the following; the emergence of bio-inspired graphene nanosystems, over time development to make them more efficient, state-of-art technology, and potential applications, mainly biomedical including biosensors, drug delivery, imaging, and biomedical systems. The outcomes of this review will certainly serve as a guideline to motivate scholars to design and develop novel bio-inspired graphene nanosystems to develop greener, affordable, and scalable next-generation biomedical systems.
    Language English
    Publishing date 2021-08-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 1362365-5
    ISSN 1361-6528 ; 0957-4484
    ISSN (online) 1361-6528
    ISSN 0957-4484
    DOI 10.1088/1361-6528/ac1bdb
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  10. Article ; Online: A comparative study on characteristics of composite (Cr3C2-NiCr) clad developed through diode laser and microwave energy.

    Hebbale, Ajit M / Kumar, Manish / Soudagar, Manzoore Elahi Mohammad / Ahamad, Tansir / Kalam, Md Abul / Mubarak, Nabisab Mujawar / Alfantazi, Akram / Khalid, Mohammad

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 10778

    Abstract: A typical ferrite/martensitic heat-resistant steel (T91) is widely used in reheaters, superheaters and power stations. ... ...

    Abstract A typical ferrite/martensitic heat-resistant steel (T91) is widely used in reheaters, superheaters and power stations. Cr
    Language English
    Publishing date 2023-07-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-37991-4
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