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Article ; Online: Robust method for chromatogram shape analysis to improve early detection of performance drifts and adverse changes in process parameters during purification column operations.

Torres, Alexis J

2023 Volume 19, Issue 1, Page(s) e2300271

Abstract: The biopharmaceutical industry is under increased pressure to maximize efficiency, enhance quality compliance, and reduce the cost of drug substance manufacturing. Ways to reduce costs associated with manufacturing of complex biological molecules include ...

Abstract	The biopharmaceutical industry is under increased pressure to maximize efficiency, enhance quality compliance, and reduce the cost of drug substance manufacturing. Ways to reduce costs associated with manufacturing of complex biological molecules include maximizing efficiency of chromatography purification steps. For example, process analytical technology (PAT) tools can be employed to improve column resin life, prevent column operating failures, and decrease the time it takes to solve investigations of process deviations. We developed a robust method to probe the shape of the chromatogram for indications of column failure or detrimental changes in the process. The approach herein utilizes raw data obtained from manufacturing followed by a pre-processing routine to align chromatograms and patch together the different chromatogram phases in preparation for multivariate analysis. A principal component analysis (PCA) was performed on the standardized chromatograms to compare different batches, and resulted in the identification specific process change that affected the profile. In addition, changes in the chromatogram peaks were used to create predictive models for impurity clearance. This approach has the potential for early detection of column processing issues, improving timely resolution in large-scale chromatographic operations.
MeSH term(s)	Chromatography ; Biological Products ; Principal Component Analysis
Chemical Substances	Biological Products
Language	English
Publishing date	2023-12-11
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2221885-3
ISSN	1860-7314 ; 1860-6768
ISSN (online)	1860-7314
ISSN	1860-6768
DOI	10.1002/biot.202300271
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Article ; Online: Oral infectivity through carnivorism in murine model of

Torres, Víctor / Contreras, Víctor / Gutiérrez, Bessy / San Francisco, Juan / Catalán, Alejandro / Vega, José Luis / Moon, Kyung-Mee / Foster, Leonard J / de Almeida, Rafael F / Kalergis, Alexis M / González, Jorge

Frontiers in cellular and infection microbiology

2024 Volume 14, Page(s) 1297099

Abstract: Introduction: Oral transmission of : Methods: Cell infectivity assays were performed on AGS cells in the presence or absence of mucin, and the roles of pepsin and acidic pH were determined. Moreover, groups of five female Balb/c mice were fed with ... ...

Abstract	Introduction: Oral transmission of Methods: Cell infectivity assays were performed on AGS cells in the presence or absence of mucin, and the roles of pepsin and acidic pH were determined. Moreover, groups of five female Balb/c mice were fed with muscle tissue obtained from mice in the acute phase of infection by the clone H510 C8C3 Results: Trypomastigote migration in the presence of mucin was reduced by approximately 30%, whereas in the presence of mucin and pepsin at pH 3.5, only a small proportion of parasites were able to migrate (∼6%). Similarly, the ability of TCTs to infect AGS cells in the presence of mucin is reduced by approximately 20%. In all cases, 60-100% of the animals were fed meat from mice infected in the acute phase or infected with trypomastigotes or amastigotes developed high parasitemia, and 80% died around day 40 post-infection. The adhesion assay showed that cruzipain is a molecule of trypomastigotes and amastigotes that binds to AGS cells. LC-MS/MS and bioinformatics analysis, also confirmed that transialidase, cysteine proteinases, and gp63 may be involved in TCTs attachment or invasion of human stomach cells because they can potentially interact with different proteins in the human stomach mucosa. In addition, several human gastric mucins have cysteine protease cleavage sites. Discussion: Then, under our experimental conditions, consuming meat from infected animals in the acute phase allows the
MeSH term(s)	Female ; Animals ; Mice ; Humans ; Trypanosoma cruzi/metabolism ; Pepsin A/metabolism ; Parasitemia ; Disease Models, Animal ; Chromatography, Liquid ; Tandem Mass Spectrometry ; Chagas Disease/parasitology ; Mucins ; Communicable Diseases
Chemical Substances	Pepsin A (EC 3.4.23.1) ; Mucins
Language	English
Publishing date	2024-02-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2024.1297099
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Article ; Online: Percutaneous closure of interventricular septal defect in a pediatric patient with dextrocardia. Clinical case report.

Aristizábal-Villa, Guillermo J / Plata-Marriaga, Alexis E / Torres-Nieto, Mary L

Archivos de cardiologia de Mexico

2022 Volume 92, Issue 2, Page(s) 289–291

Title translation	Cierre percutáneo de comunicación interventricular en paciente pediátrico con dextrocardia. Reporte de caso clínico.
MeSH term(s)	Cardiac Catheterization ; Child ; Dextrocardia/complications ; Heart Septal Defects, Atrial ; Heart Septal Defects, Ventricular/surgery ; Humans ; Septal Occluder Device ; Treatment Outcome
Language	Spanish
Publishing date	2022-04-04
Publishing country	Mexico
Document type	Case Reports ; Journal Article
ISSN	1665-1731
ISSN (online)	1665-1731
DOI	10.24875/ACM.21000087
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Article ; Online: Medical students' knowledge about human papillomavirus (HPV), HPV vaccine and head and neck cancer.

Solis-Torres, Norangelys / Braverman-Diaz, Isardo / Rivera-Morales, Luis A / Perez-Sanchez, Jose J / Perez-Bravo, Valeria S / Neris-Sanchez, Angel J / Vera, Alexis / Diaz-Algorri, Yaritza

Human vaccines & immunotherapeutics

2024 Volume 20, Issue 1, Page(s) 2344248

Abstract: The Human Papillomavirus (HPV) is a sexually transmitted infection that significantly affects the population worldwide. HPV preventive methods include vaccination, prophylactics, and education. Different types of cancers associated with HPV usually take ... ...

Abstract	The Human Papillomavirus (HPV) is a sexually transmitted infection that significantly affects the population worldwide. HPV preventive methods include vaccination, prophylactics, and education. Different types of cancers associated with HPV usually take years or decades to develop after infections, such as Head and Neck Cancer(HNC). Therefore, HPV prevention can be considered cancer prevention. A sample of medical students in Puerto Rico was evaluated to assess their knowledge about HPV, HPV vaccine, and HNC through two previously validated online questionnaires composed of 38 dichotomized questions, we measured HPV, HPV vaccination(HPVK), and HNC knowledge (HNCK). Out of 104 students surveyed, the mean HPVK score obtained was 20.07/26, SD = 3.86, while the mean score for HNCK was 6.37/12, SD = 1.78. Bidirectional stepwise regression showed study year and HPV Vaccine name had been the most influential variables on HPVK and HNCK. MS1 participants scored lower than MS2-MS4 participants, with no significant difference between MS2-MS4 scores. The results reveal knowledge gaps in HPV/HPV Vaccine and HNC among surveyed medical students. Our findings also suggest an association between knowledge of personal vaccination status, self-perceived risk, and how uncertainty in these factors may affect the medical students' understanding of HPV, HPV vaccination, and associated cancers.
MeSH term(s)	Humans ; Students, Medical/psychology ; Students, Medical/statistics & numerical data ; Papillomavirus Vaccines/administration & dosage ; Papillomavirus Infections/prevention & control ; Health Knowledge, Attitudes, Practice ; Female ; Male ; Surveys and Questionnaires ; Head and Neck Neoplasms/prevention & control ; Young Adult ; Puerto Rico ; Vaccination/psychology ; Vaccination/statistics & numerical data ; Adult ; Human Papillomavirus Viruses
Chemical Substances	Papillomavirus Vaccines
Language	English
Publishing date	2024-04-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	2664176-8
ISSN	2164-554X ; 2164-5515
ISSN (online)	2164-554X
ISSN	2164-5515
DOI	10.1080/21645515.2024.2344248
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Article ; Online: Testing locus coeruleus-norepinephrine accounts of working memory, attention control, and fluid intelligence.

Robison, Matthew K / Ralph, Kathryn J / Gondoli, Dawn M / Torres, Alexis / Campbell, Stephen / Brewer, Gene A / Gibson, Bradley S

Cognitive, affective & behavioral neuroscience

2023 Volume 23, Issue 4, Page(s) 1014–1058

Abstract: The current set of studies examined the relationship among working memory capacity, attention control, fluid intelligence, and pupillary correlates of tonic arousal regulation and phasic responsiveness in a combined sample of more than 1,000 participants ...

Abstract	The current set of studies examined the relationship among working memory capacity, attention control, fluid intelligence, and pupillary correlates of tonic arousal regulation and phasic responsiveness in a combined sample of more than 1,000 participants in two different age ranges (young adults and adolescents). Each study was designed to test predictions made by two recent theories regarding the role of the locus coeruleus-norepinephrine (LC-NE) system in determining individual differences in cognitive ability. The first theory, proposed by Unsworth and Robison (2017a), posits two important individual differences: the moment-to-moment regulation of tonic arousal, and the phasic responsiveness of the system to goal-relevant stimuli. The second theory, proposed by Tsukahara and Engle (2021a), argues that people with higher cognitive abilities have greater functional connectivity between the LC-NE system and cortical networks at rest. These two theories are not mutually exclusive, but they make different predictions. Overall, we found no evidence consistent with a resting-state theory. However, phasic responsiveness was consistently correlated with working memory capacity, attention control, and fluid intelligence, supporting a prediction made by Unsworth and Robison (2017a). Tonic arousal regulation was not correlated with working memory or fluid intelligence and was inconsistently correlated with attention control, which offers only partial support for Unsworth and Robison's (2017a) second prediction.
MeSH term(s)	Humans ; Adolescent ; Memory, Short-Term/physiology ; Norepinephrine/physiology ; Locus Coeruleus/physiology ; Attention/physiology ; Intelligence
Chemical Substances	Norepinephrine (X4W3ENH1CV)
Language	English
Publishing date	2023-04-20
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
ZDB-ID	2029088-3
ISSN	1531-135X ; 1530-7026
ISSN (online)	1531-135X
ISSN	1530-7026
DOI	10.3758/s13415-023-01096-2
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Article: Physiologic and pharmacologic considerations in morbid obesity and bariatric anesthesia.

Brondeel, Kimberley C / Lakatta, Alexis C / Torres, Grant B / Hurley, Joshua J / Kunik, Illan L / Haney, Kaley F / Cornett, Elyse M / Kaye, Alan D

Saudi journal of anaesthesia

2022 Volume 16, Issue 3, Page(s) 306–313

Abstract: Obesity is a growing worldwide health hazard that is characterized by excess malnutrition. Excess food intake leads to dysregulated energy homeostasis and increased adiposity, activating pro-inflammatory physiologic pathways that can contribute to the ... ...

Abstract	Obesity is a growing worldwide health hazard that is characterized by excess malnutrition. Excess food intake leads to dysregulated energy homeostasis and increased adiposity, activating pro-inflammatory physiologic pathways that can contribute to the chronic inflammatory state associated with many chronic illnesses. Obesity is a preventable illness, but its multifaceted etiology, including genetic, behavioral, and environmental variables, is critical to understanding its epidemiology and pathophysiology. Obesity is a critical predisposing factor for illnesses including type II diabetes, cardiovascular disease, and cancer, with higher morbidity and death. Obesity rates are rising, and so will the need for perioperative anesthesia for subjects with obesity. Obesity epidemiology, biochemistry, and pathophysiology are significant concepts in perioperative anesthesia management for subjects with obesity. To provide optimal intraoperative care for subjects with obesity, preoperative cardiovascular assessment for coronary artery disease and drug monitoring is required. Individuals suffering from obesity have significantly higher oxygen consumption rates and a higher risk of desaturation and surgical complications. Individuals suffering from obesity require specialized perioperative treatment related to higher prevalence of perioperative complications.
Language	English
Publishing date	2022-06-20
Publishing country	India
Document type	Journal Article ; Review
ZDB-ID	2562174-9
ISSN	0975-3125 ; 1658-354X
ISSN (online)	0975-3125
ISSN	1658-354X
DOI	10.4103/sja.sja_185_22
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Article ; Online: Survival according to the site of metastasis in triple-negative breast cancer patients: The Peruvian experience.

Piedra-Delgado, Luis / Chambergo-Michilot, Diego / Morante, Zaida / Fairen, Carlos / Jerves-Coello, Fernando / Luque-Benavides, Renato / Casas, Fresia / Bustamante, Eduarda / Razuri-Bustamante, Cesar / Torres-Roman, J Smith / Fuentes, Hugo / Gomez, Henry / Narvaez-Rojas, Alexis / De la Cruz-Ku, Gabriel / Araujo, Jhajaira

PloS one

2024 Volume 19, Issue 2, Page(s) e0293833

Abstract: Background: Evidence regarding differences in survival associated with the site of metastasis in triple-negative breast cancer (TNBC) remains limited. Our aim was to analyze the overall survival (OS), distant relapse free survival (DRFS), and survival ... ...

Abstract	Background: Evidence regarding differences in survival associated with the site of metastasis in triple-negative breast cancer (TNBC) remains limited. Our aim was to analyze the overall survival (OS), distant relapse free survival (DRFS), and survival since the diagnosis of the relapse (MS), according to the side of metastasis. Methods: This was a retrospective study of TNBC patients with distant metastases at the Instituto Nacional de Enfermedades Neoplasicas (Lima, Peru) from 2000 to 2014. Prognostic factors were determined by multivariate Cox regression analysis. Results: In total, 309 patients were included. Regarding the type of metastasis, visceral metastasis accounted for 41% and the lung was the most frequent first site of metastasis (33.3%). With a median follow-up of 10.2 years, the 5-year DRFS and OS were 10% and 26%, respectively. N staging (N2-N3 vs. N0, HR = 1.49, 95%CI: 1.04-2.14), metastasis in visceral sites (vs. bone; HR = 1.55, 95%CI: 0.94-2.56), the central nervous system (vs. bone; HR = 1.88, 95% CI: 1.10-3.22), and multiple sites (vs. bone; HR = 2.55, 95%CI:1.53-4.25) were prognostic factors of OS whereas multiple metastasis (HR = 2.30, 95% CI: 1.42-3.72) was a predictor of MS. In terms of DRFS, there were no differences according to metastasis type or solid organ. Conclusion: TNBC patients with multiple metastasis and CNS metastasis have an increased risk of death compared to those with bone metastasis in terms of OS and MS.
MeSH term(s)	Humans ; Female ; Triple Negative Breast Neoplasms/pathology ; Peru/epidemiology ; Neoplasm Staging ; Retrospective Studies ; Breast Neoplasms/pathology ; Neoplasm Recurrence, Local/pathology ; Prognosis
Language	English
Publishing date	2024-02-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0293833
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Article: Typification of Dactylis ibizensis (Poaceae) and inference of the ploidy level of the type specimen

Rosselló, Josep A. / Maravilla, Alexis J. / Rosato, Marcela / Torres, Nèstor / Ferrer‐Gallego, P. Pablo

Taxon. 2021 Aug., v. 70, no. 4

2021

Abstract: Dactylis ibizensis (Poaceae) is lectotypified based on a specimen at W. Anatomical and macromorphological data from karyologically verified individuals strongly support that the lectotype comprises exclusively diploid plants. This allows for preservation ...

Abstract	Dactylis ibizensis (Poaceae) is lectotypified based on a specimen at W. Anatomical and macromorphological data from karyologically verified individuals strongly support that the lectotype comprises exclusively diploid plants. This allows for preservation of the name in current use for the Balearic cocksfoot restricted to the islands of Formentera and Ibiza.
Keywords	Dactylis glomerata ; diploidy ; lectotypes
Language	English
Dates of publication	2021-08
Size	p. 866-874.
Publishing place	John Wiley & Sons, Inc.
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	204216-2
ISSN	0040-0262
ISSN	0040-0262
DOI	10.1002/tax.12507
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Article ; Online: RUScal: Software for the analysis of resonant ultrasound spectroscopy measurements.

Torres, James / Flores-Betancourt, Alexis / Hermann, Raphaël P

The Journal of the Acoustical Society of America

2022 Volume 151, Issue 5, Page(s) 3547

Abstract: Resonant ultrasound spectroscopy is used to nondestructively measure the elastic resonances of small solids to elucidate the material's elastic properties or other qualities like size, shape, or composition. Here, we introduce the software RUScal for the ...

Abstract	Resonant ultrasound spectroscopy is used to nondestructively measure the elastic resonances of small solids to elucidate the material's elastic properties or other qualities like size, shape, or composition. Here, we introduce the software RUScal for the purpose of determining elastic properties by analyzing the eigenfrequencies of solid specimens with common shapes, such as rectangular parallelepipeds, cylinders (solid and hollow tube), ellipsoids, and octahedrons, as well as irregularly shaped ellipsoids that can be described analytically. All symmetry classes are supported, from isotropic to triclinic, along with the option to add or remove up to three orthogonal mirror planes as well as the ability to reorient the crystal axes with respect the sample edges via Euler angles. Additional features include tools to help find initial sets of elastic constants, including grid exploration and Monte Carlo methods, a tool to analyze frequencies as a function of sample length or crystal orientation, an error analysis tool to assess fit quality, and formatting of the input and output files for batch fitting, e.g., as a function of temperature. This software was validated with published resonant ultrasound spectroscopy data for various materials, shapes, and symmetries with noted improvements in calculation time compared to finite element methods.
Language	English
Publishing date	2022-05-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	219231-7
ISSN	1520-8524 ; 0001-4966
ISSN (online)	1520-8524
ISSN	0001-4966
DOI	10.1121/10.0011397
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Article ; Online: Low Carbohydrate Diets and Estimated Cardiovascular and Metabolic Syndrome Risk in Prostate Cancer.

Freedland, Stephen J / Howard, Lauren E / Ngo, Alexis / Ramirez-Torres, Adela / Csizmadi, Ilona / Cheng, Susan / Mack, Alexandra / Lin, Pao-Hwa

The Journal of urology

2021 Volume 206, Issue 6, Page(s) 1411–1419

Abstract: Purpose: A low carbohydrate diet (LCD) was shown to suggestively slow prostate cancer (PC) growth. In noncancer patients, LCDs improve metabolic syndrome (MetS) without weight loss. However, concerns about negative impact on cardiovascular disease (CVD) ...

Abstract	Purpose: A low carbohydrate diet (LCD) was shown to suggestively slow prostate cancer (PC) growth. In noncancer patients, LCDs improve metabolic syndrome (MetS) without weight loss. However, concerns about negative impact on cardiovascular disease (CVD) risk remain. The objective of this secondary analysis is to determine the impact of an LCD on risk of MetS and estimated CVD risk in patients with PC. Materials and methods: Pooled data were analyzed from 2 randomized trials testing LCD vs control on 1) preventing insulin resistance after starting hormone therapy (CAPS1) and 2) slowing PC growth in recurrent PC after failed primary treatment (CAPS2). Both trials included a usual care control vs LCD intervention in which patients were instructed to limit carbohydrate intake to ≤20 gm/day, and in CAPS1 only, to walk for ≥30 minutes/day for ≥5 days/week. MetS components (hypertension, high triglycerides, low high-density lipoprotein cholesterol, central obesity and diabetes), 10-year CVD risk estimated using the Framingham Score with either body mass index (BMI) or lipids, and remnant cholesterol were compared between arms using mixed models adjusting for trial. Results: LCD resulted in a significantly reduced risk of MetS (p=0.004) and remnant cholesterol (p <0.001). Moreover, LCD resulted in significantly lower estimated CVD risk using BMI (p=0.002) over the study with no difference in estimated CVD risk using lipids (p=0.14). Conclusions: LCD resulted in a significantly reduced risk of MetS and remnant cholesterol, and a significantly lower estimated CVD risk using BMI. By comparison, there was no difference in estimated CVD risk using lipids. Study limitations include small sample size, short followup, and inability to distinguish effects of carbohydrate restriction and weight loss. Long-term studies are needed to confirm this finding.
MeSH term(s)	Aged ; Cardiovascular Diseases/epidemiology ; Cardiovascular Diseases/etiology ; Diet, Carbohydrate-Restricted/adverse effects ; Humans ; Male ; Metabolic Syndrome/epidemiology ; Metabolic Syndrome/prevention & control ; Middle Aged ; Prostatic Neoplasms/complications ; Randomized Controlled Trials as Topic ; Risk Assessment
Language	English
Publishing date	2021-07-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	3176-8
ISSN	1527-3792 ; 0022-5347
ISSN (online)	1527-3792
ISSN	0022-5347
DOI	10.1097/JU.0000000000002112
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