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Article ; Online: Genomic analysis reveals a rapid spread and predominance of lambda (C.37) SARS-COV-2 lineage in Peru despite circulation of variants of concern.

Padilla-Rojas, Carlos / Jimenez-Vasquez, Victor / Hurtado, Veronica / Mestanza, Orson / Molina, Iris S / Barcena, Luis / Morales Ruiz, Sandra / Acedo, Steve / Lizarraga, Wendy / Bailon, Henri / Cáceres, Omar / Galarza, Marco / Rojas-Serrano, Nancy / Vargas-Herrera, Natalia / Lope-Pari, Priscila / Huayra, Joseph / Solari, Lely

Journal of medical virology

2021 Volume 93, Issue 12, Page(s) 6845–6849

Abstract: ... of the lambda lineage (C.37) until becoming predominant between January and April 2021, despite ...

Abstract	The pandemic generated by SARS-Cov-2 has caused a large number of cases and deaths in the world, but South America has been one of the continents that were most hard hit. The appearance of new variants causes concern because of the possibility that they may evade the protection generated by vaccination campaigns, their greater capacity to be transmitted, or their higher virulence. We analyzed the circulating variants in Peru after improving our Genomic Surveillance program. The results indicate a steep increase of the lambda lineage (C.37) until becoming predominant between January and April 2021, despite the cocirculation of other variants of concern or interest. Lambda lineage deserves close monitoring and could probably become a variant of concern in the near future.
MeSH term(s)	COVID-19/epidemiology ; COVID-19/virology ; Genome, Viral/genetics ; Genomics/methods ; Humans ; Mutation/genetics ; Pandemics/prevention & control ; Peru/epidemiology ; SARS-CoV-2/genetics
Language	English
Publishing date	2021-08-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.27261
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Article ; Online: Identification of the Gamma variant in an outbreak of COVID-19 at a prison in Peru.

Vargas-Herrera, N / Roque de la Piedra, S / Padilla-Rojas, C / Yagui-Moscoso, M

Revista espanola de sanidad penitenciaria

2022 Volume 23, Issue 3, Page(s) 128–129

MeSH term(s)	COVID-19/epidemiology ; Humans ; Peru/epidemiology ; Prisons ; SARS-CoV-2
Language	English
Publishing date	2022-04-12
Publishing country	Spain
Document type	Letter
ZDB-ID	2424262-7
ISSN	2013-6463 ; 2013-6463
ISSN (online)	2013-6463
ISSN	2013-6463
DOI	10.18176/resp.00042
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Article ; Online: Cloning, expression and seroreactivity of the recombinant lipopolysaccharide assembly protein - D (LptD) from Bartonella bacilliformis.

Flores-Nuñez, Astrid / Ventura, Gladis / Bailon, Henri / Marcelo, Adolfo / Sandoval, Gustavo / Padilla-Rojas, Carlos

Revista peruana de medicina experimental y salud publica

2022 Volume 39, Issue 1, Page(s) 15–23

Abstract: Objective.: To evaluate in silico and at the serological level the antigenic potential of the recombinant extracellular domain of the lipopolysaccharide assembly protein - D (LptD) of Bartonella bacilliformis (dexr_LptD).: Materials and methods.: ... ...

Title translation	Clonamiento, expresión y seroreactividad de la proteína recombinante de ensamblaje de lipopolisacáridos - D (LptD) de Bartonella bacilliformis.
Abstract	Objective.: To evaluate in silico and at the serological level the antigenic potential of the recombinant extracellular domain of the lipopolysaccharide assembly protein - D (LptD) of Bartonella bacilliformis (dexr_LptD). Materials and methods.: Through in silico analysis, we selected a B. bacilliformis protein with antigenic and immunogenic potential. The selected protein gene was cloned into Escherichia coli TOP10 and expressed in Escherichia coli BL21 (DE3) pLysS. Recombinant protein was expressed using isopropyl-β-D-1-thiogalactopyranoside (IPTG) and induction conditions were optimized. Finally, it was purified with Ni-IDA resin (His60 Ni Superflow) and a Western Blot assay was conducted. Results.: In silico, the selected protein was LptD because it is located in the outer membrane and is antigenic and immunogenic. Optimized conditions for dexr_LptD induction were 0.5 mM IPTG, 16 hours, TB (Terrific Broth) medium, 3% (v/v) ethanol, 28 ºC, OD600: 1-1.5 and 200 rpm. Purification was carried out under denaturating conditions on a small scale and we obtained 2.6 μg/mL of partially purified dexr_LptD. The Western Blot assay showed a positive reaction between the sera from patients with Carrión's Disease and dexr_LptD, which shows the antigenicity of dexr_LptD. Conclusions.: The dexr_LptD shows antigenicity both in silico and at the serological level, these results are the basis for further studies on vaccine candidates against Carrion's Disease.
MeSH term(s)	Bacterial Outer Membrane Proteins/genetics ; Bacterial Outer Membrane Proteins/metabolism ; Bartonella Infections ; Bartonella bacilliformis/genetics ; Cloning, Molecular ; Escherichia coli/genetics ; Escherichia coli Proteins/genetics ; Humans ; Isopropyl Thiogalactoside/metabolism ; Lipopolysaccharides/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism
Chemical Substances	Bacterial Outer Membrane Proteins ; Escherichia coli Proteins ; Lipopolysaccharides ; LptD protein, E coli ; Recombinant Proteins ; Isopropyl Thiogalactoside (367-93-1)
Language	English
Publishing date	2022-06-24
Publishing country	Peru
Document type	Journal Article
ZDB-ID	2120092-0
ISSN	1726-4642 ; 1726-4642
ISSN (online)	1726-4642
ISSN	1726-4642
DOI	10.17843/rpmesp.2022.391.9292
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Article ; Online: Sub-lineage B.1.6 of hMPXV in a global context: Phylogeny and epidemiology.

Molina, Iris S / Jimenez-Vasquez, Victor / Lizarraga, Wendy / Sevilla, Nieves / Hurtado, Veronica / Padilla-Rojas, Carlos

Journal of medical virology

2023 Volume 95, Issue 9, Page(s) e29056

Abstract: During the 2022 COVID-19 pandemic, monkeypox emerged as a significant threat to global health. The virus responsible for the disease, the human monkeypox virus (hMPXV), underwent various genetic changes, resulting in the emergence of over a dozen ... ...

Abstract	During the 2022 COVID-19 pandemic, monkeypox emerged as a significant threat to global health. The virus responsible for the disease, the human monkeypox virus (hMPXV), underwent various genetic changes, resulting in the emergence of over a dozen distinct lineages, which could be identified by only a small number of unique mutations. As of January 25, 2023, genomic information of hMPXV generated had reached 4632 accessions in the GISAID database. In this study, we aimed to investigate the epidemiological and phylogenetic characteristics of the B.1.6 sub-lineage of hMPXV in Peru, compared with other circulating sub-lineages during the global outbreak. The B.1.6 sub-lineage, characterized by the 111029G>A mutation, was estimated to have emerged in June 2022 and was found mainly in Peru. Most cases (95.8%) were men with an average age of 33 years, and nearly half of the patients had HIV, of whom only 77.35% received antiretroviral therapy. Our findings revealed that the B.1.6, B.1.4, and B.1.2 sub-lineages were well represented and had a higher number of mutations despite having the lowest media substitution rates per site per year. Moreover, it was estimated that B.1.2 and B.1.4 appeared in February 2022 and were the first two sub-lineages to emerge. A mutation profile was also obtained for each sub-lineage, reflecting that several mutations had a pattern similar to the characteristic mutation. This study provides the first estimation of the substitution rate and ancestry of each monkeypox sub-lineage belonging to the 2022 outbreak. Based on our findings, continued genomic surveillance of monkeypox is necessary to understand better and track the evolution of the virus.
MeSH term(s)	Male ; Humans ; Adult ; Female ; Phylogeny ; Mpox (monkeypox) ; Pandemics ; COVID-19 ; Databases, Factual
Language	English
Publishing date	2023-09-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.29056
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Article ; Online: Reinfections Are More Frequent Than Currently Considered in Countries With High Incidence of Coronavirus Disease 2019 (COVID-19) Cases Due to Stringent Definitions.

Pampa-Espinoza, Luis / Silva-Valencia, Javier / Fernandez-Navarro, Manuel / Padilla-Rojas, Carlos / Solari, Lely

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

2021 Volume 74, Issue 8, Page(s) 1505–1506

MeSH term(s)	COVID-19 ; Humans ; Incidence ; Reinfection ; SARS-CoV-2
Language	English
Publishing date	2021-07-10
Publishing country	United States
Document type	Letter ; Research Support, Non-U.S. Gov't ; Comment
ZDB-ID	1099781-7
ISSN	1537-6591 ; 1058-4838
ISSN (online)	1537-6591
ISSN	1058-4838
DOI	10.1093/cid/ciab783
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Article ; Online: A Trimetallic Pt 2 NiCo/C Electrocatalyst with Enhanced Activity and Durability for Oxygen Reduction Reaction

Hilda M. Alfaro-López / Manuel A. Valdés-Madrigal / Hugo Rojas-Chávez / Heriberto Cruz-Martínez / Miguel A. Padilla-Islas / Miriam M. Tellez-Cruz / Omar Solorza-Feria

Catalysts, Vol 10, Iss 2, p

2020 Volume 170

Abstract: ... the physical properties of the Pt 2 NiCo/C nanocatalyst by X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy ... whereas the catalytic activities of the Pt 2 NiCo/C and Pt/C nanocatalysts were determined through cyclic voltammetry (CV), CO ... for the synthesized nanocatalyst is ~6.4-fold higher than that of Pt/C alone, and its mass activity is ~2.2-fold ...

Abstract	Commercialization of the polymer electrolyte membrane fuel cell (PEMFC) requires that electrocatalysts for oxygen reduction reaction (ORR) satisfy two main considerations: materials must be highly active and show long-term stability in acid medium. Here, we describe the synthesis, physical characterization, and electrochemical evaluation of carbon-dispersed Pt 2 NiCo nanocatalysts for ORR in acid medium. We synthesized a trimetallic electrocatalyst via chemical route in organic medium and investigated the physical properties of the Pt 2 NiCo/C nanocatalyst by X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy-scanning electron microscope (EDXS-SEM), and scanning transmission electron microscopy (STEM), whereas the catalytic activities of the Pt 2 NiCo/C and Pt/C nanocatalysts were determined through cyclic voltammetry (CV), CO-stripping, and rotating disk electrode (RDE) electrochemical techniques. XRD and EDXS-SEM results confirmed the presence of the three metals in the nanoparticles, and scanning transmission electron microscopy (STEM) allowed observation of the Pt 2 NiCo nanoparticles at ~10 nm. The measured specific activity for the synthesized nanocatalyst is ~6.4-fold higher than that of Pt/C alone, and its mass activity is ~2.2-fold higher than that of Pt/C, which is attributed to the synergistic interaction of the trimetallic electrocatalyst. Furthermore, the specific and mass activities of the synthesized material are maintained after the accelerated stability test, whereas the catalytic properties of Pt/C decreased. These results suggest that the Pt 2 NiCo/C trimetallic nanocatalyst is a promising candidate cathode electrode for use in PEMFCs.
Keywords	trimetallic nanoparticles ; mass activity ; specific activity ; stability ; Chemical technology ; TP1-1185 ; Chemistry ; QD1-999
Subject code	620 ; 540
Language	English
Publishing date	2020-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article ; Online: π-stacking and C-X...D (X = H, NO2; D = O, π) interactions in the crystal network of both C-H...N and π-stacked dimers of 1,2-bis(4-bromophenyl)-1H-benzimidazole and 2-(4-bromophenyl)-1-(4-nitrophenyl)-1H-benzimidazole.

González-Padilla, Jazmin E / Rosales-Hernández, Martha C / Padilla-Martínez, Itzia I / García-Báez, Efren V / Rojas-Lima, Susana / Salazar-Pereda, Veronica

Acta crystallographica. Section C, Structural chemistry

2014 Volume 70, Issue Pt 1, Page(s) 55–59

Abstract: ... nitrophenyl)-1H-benzimidazole, C19H12BrN3O2, (II), are arranged in dimeric units through C-H...N and parallel ... displaced π-stacking interactions favoured by the appropriate disposition of N- and C-bonded phenyl rings ... by the concurrence of a diverse set of weak intermolecular C-X...D (X = H, NO2; D = O, π) interactions. ...

Abstract	Molecules of 1,2-bis(4-bromophenyl)-1H-benzimidazole, C19H12Br2N2, (I), and 2-(4-bromophenyl)-1-(4-nitrophenyl)-1H-benzimidazole, C19H12BrN3O2, (II), are arranged in dimeric units through C-H...N and parallel-displaced π-stacking interactions favoured by the appropriate disposition of N- and C-bonded phenyl rings with respect to the mean benzimidazole plane. The molecular packing of the dimers of (I) and (II) arises by the concurrence of a diverse set of weak intermolecular C-X...D (X = H, NO2; D = O, π) interactions.
MeSH term(s)	Benzimidazoles/chemistry ; Hydrogen Bonding ; Models, Molecular ; Nitro Compounds/chemistry
Chemical Substances	2-(4-bromophenyl)-1-(4-nitrophenyl)-1H-benzimidazole ; Benzimidazoles ; Nitro Compounds ; benzimidazole (E24GX49LD8)
Language	English
Publishing date	2014-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2025703-X
ISSN	2053-2296 ; 1600-5759 ; 0108-2701
ISSN (online)	2053-2296 ; 1600-5759
ISSN	0108-2701
DOI	10.1107/S2053229613033329
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Article ; Online: PTPN22 -1123G>C polymorphism and anti-cyclic citrullinated protein antibodies in rheumatoid arthritis.

Muñoz-Valle, José Francisco / Padilla-Gutiérrez, Jorge Ramón / Hernández-Bello, Jorge / Ruiz-Noa, Yeniley / Valle, Yeminia / Palafox-Sánchez, Claudia Azucena / Parra-Rojas, Isela / Gutiérrez-Ureña, Sergio Ramón / Rangel-Villalobos, Hector

Medicina clinica

2017 Volume 149, Issue 3, Page(s) 95–100

Abstract: ... been associated with different autoimmune disorders. The PTPN22 -1123G>C polymorphism appears to affect ... arthritis (RA) risk remains unknown. We evaluate the association of PTPN22 -1123G>C polymorphism with anti ... to ACR-EULAR criteria and 300 control subjects (CS) was conducted. The -1123 G>C polymorphism was ...

Title translation	Polimorfismo −1123G>C en el gen PTPN22 y anticuerpos antipéptido citrulinado cíclico en la artritis reumatoide.
Abstract	Background and objectives: The protein tyrosine phosphatase non-receptor type 22 (PTPN22) gene encodes an important negative regulator of T-cell activation, lymphoid-specific phosphatase -Lyp- and has been associated with different autoimmune disorders. The PTPN22 -1123G>C polymorphism appears to affect the transcriptional control of this gene, but to date, the biological significance of this polymorphisms on rheumatoid arthritis (RA) risk remains unknown. We evaluate the association of PTPN22 -1123G>C polymorphism with anti-cyclic citrullinated protein antibodies (anti-CCP) and risk for RA in population from Western Mexico. Material and methods: A transversal analytic study, which enrolled 300 RA patients classified according to ACR-EULAR criteria and 300 control subjects (CS) was conducted. The -1123 G>C polymorphism was genotyped by PCR-RFLP. The anti-CCP antibodies levels were quantified by ELISA kit. Results: We found a higher prevalence of homozygous PTPN22 -1123CC genotype in CS than in RA patients (OR 0.41; 95% confidence interval 0.24-0.71; P=.001), suggesting a potential protective effect against RA. Concerning anti-CCP levels, the CC genotype carriers showed the lowest median levels in RA (P<.05). Conclusion: The PTPN22 -1123CC genotype is a protector factor to RA in a Mexican-mestizo population and is associated with low anti-CCP antibodies.
Language	Spanish
Publishing date	2017-08-10
Publishing country	Spain
Document type	Journal Article
ZDB-ID	411607-0
ISSN	1578-8989 ; 0025-7753
ISSN (online)	1578-8989
ISSN	0025-7753
DOI	10.1016/j.medcli.2017.01.025
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Article ; Online: Macrophage migration inhibitory factor: association of -794 CATT5-8 and -173 G>C polymorphisms with TNF-α in systemic lupus erythematosus.

De la Cruz-Mosso, U / Bucala, R / Palafox-Sánchez, C A / Parra-Rojas, I / Padilla-Gutiérrez, J R / Pereira-Suárez, A L / Rangel-Villalobos, H / Vázquez-Villamar, M / Angel-Chávez, L I / Muñoz-Valle, J F

Human immunology

2014 Volume 75, Issue 5, Page(s) 433–439

Abstract: ... rs5844572) and -173 G>C (rs755622) MIF polymorphisms was performed by PCR and PCR-RFLP, respectively in 186 ... a significant association of genotypes carrying the -794 CATT7 and -173(∗)C risk alleles with susceptibility ...

Abstract	Macrophage migration inhibitory factor (MIF) is an upstream immunoregulatory cytokine associated with the pathogenesis of autoimmune inflammatory diseases. There is evidence that MIF functions in a positive feedback loop with TNF-α that could perpetuate the inflammatory process in systemic lupus erythematosus (SLE). In this case-control study we investigated whether commonly occurring functional MIF polymorphisms are associated with SLE as well as with MIF and TNF-α serum levels in a Mexican-Mestizo population. Genotyping of the -794 CATT5-8 (rs5844572) and -173 G>C (rs755622) MIF polymorphisms was performed by PCR and PCR-RFLP, respectively in 186 SLE patients and 200 healthy subjects. MIF and TNF-α serum levels were determined by ELISA. A significant increase of MIF and TNF-α levels was found in SLE patients. According to a genetic model, we found a significant association of genotypes carrying the -794 CATT7 and -173(∗)C risk alleles with susceptibility to SLE and with a significant increase of TNF-α. In conclusion, MIF gene polymorphisms are associated with SLE susceptibility and with an increase of TNF-α serum levels in a Mexican-Mestizo population.
MeSH term(s)	Adolescent ; Adult ; Aged ; Alleles ; Case-Control Studies ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Humans ; Lupus Erythematosus, Systemic/blood ; Lupus Erythematosus, Systemic/genetics ; Macrophage Migration-Inhibitory Factors/blood ; Macrophage Migration-Inhibitory Factors/genetics ; Male ; Mexico ; Middle Aged ; Odds Ratio ; Polymorphism, Single Nucleotide ; Promoter Regions, Genetic ; Tumor Necrosis Factor-alpha/blood ; Tumor Necrosis Factor-alpha/genetics ; Young Adult
Chemical Substances	Macrophage Migration-Inhibitory Factors ; Tumor Necrosis Factor-alpha
Language	English
Publishing date	2014-02-12
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	801524-7
ISSN	1879-1166 ; 0198-8859
ISSN (online)	1879-1166
ISSN	0198-8859
DOI	10.1016/j.humimm.2014.02.014
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Article ; Online: Validation and evaluation of RT-PCR real time in house test to detection of SARS-CoV-2 using specific RdRp gene and GAPDH endogen control.

Rojas-Serrano, Nancy / Lope-Pari, Priscila / Huaringa-Nuñez, Maribel / Marques Simas, Paulo Vitor / Palacios-Salvatierra, Rosa / Balbuena-Torres, Johanna / Caceres Rey, Omar Alberto / Padilla-Rojas, Carlos

Revista peruana de medicina experimental y salud publica

2022 Volume 38, Issue 4, Page(s) 595–600

Abstract: The present work validated and evaluated a duplex real-time RT-PCR using specific primers and probes for genes RdRp from SARS-CoV-2 and GAPDH from humans; the latter was used as an endogenous control in all reactions. We evaluated the specificity, the ... ...

Title translation	Validación y evaluación de una prueba de RT-PCR en tiempo real in house para la detección de SARS-CoV-2 usando un gen específico RdRp y control endógeno GAPDH.
Abstract	The present work validated and evaluated a duplex real-time RT-PCR using specific primers and probes for genes RdRp from SARS-CoV-2 and GAPDH from humans; the latter was used as an endogenous control in all reactions. We evaluated the specificity, the sensitivity, the robustness, the reproducibility, the repeatability, the comparability, and the limit of detection. The predictive positive and negative values (PPV and PNV, respectively) and all the parameters evaluated using our duplex real-time RT-PCR was 100%. The detection limit was 100 copies/µL according to the acceptance criteria established for the validation of this protocol. Our duplex real-time RT-PCR demonstrated to be a good alternative for the diagnosis of COVID-19; in addition, this PCR was used adequately in suspicion of COVID-19, allowing it to control the number of false-negatives.
MeSH term(s)	COVID-19/diagnosis ; COVID-19 Testing/methods ; COVID-19 Testing/standards ; Humans ; RNA, Viral/genetics ; RNA-Dependent RNA Polymerase ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2/genetics ; Sensitivity and Specificity
Chemical Substances	RNA, Viral ; RNA-Dependent RNA Polymerase (EC 2.7.7.48)
Language	English
Publishing date	2022-04-01
Publishing country	Peru
Document type	Journal Article
ZDB-ID	2120092-0
ISSN	1726-4642 ; 1726-4642
ISSN (online)	1726-4642
ISSN	1726-4642
DOI	10.17843/rpmesp.2021.384.7596
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