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Article ; Online: Two-dimensional echocardiographic measures of left ventricular dimensions agree with M-mode measurements in dogs.

Rishniw, M / Corda, A / Spina, F / Caivano, D

Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology

2020 Volume 33, Page(s) 69–75

Abstract: Introduction: Clinicians measure left ventricular dimensions in dogs from both M-mode and two ... dimensions from archived M-mode and two-dimensional images obtained from the right parasternal short-axis ... with two-dimensional measurements being progressively larger than M-mode measurements as ventricular size ...

Abstract	Introduction: Clinicians measure left ventricular dimensions in dogs from both M-mode and two-dimensional images. Little information currently exists as to whether these two methods provide measurements similar enough to be interchangeable. Animals: The animals included in this study are 206 client-owned dogs: 68 healthy, 105 with myxomatous mitral valve disease, 33 with other cardiac or extracardiac disease. Materials and methods: Investigators measured left ventricular diastolic and systolic dimensions from archived M-mode and two-dimensional images obtained from the right parasternal short-axis view. Agreement between the pairs of measurements was examined using limits of agreement (Bland-Altman) plots. Results: Left ventricular diastolic dimensions showed no fixed or proportional bias but did show heteroscedasticity. Ninety-five percent limits of agreement for normalized differences approximated ±10%; 95% of the absolute differences for any pair of measurements were <3.9 mm regardless of bodyweight and <2.7 mm for dogs <15 kg. Left ventricular systolic dimensions showed slight proportional bias, with two-dimensional measurements being progressively larger than M-mode measurements as ventricular size increased. Ninety-five percent limits of agreement for normalized differences approximated ±20%; 95% of the absolute differences for any pair of measurements were <4.6 mm regardless of bodyweight and <3.5 mm for dogs <15 kg. Mitral valve disease did not appreciably affect these findings. Conclusions: Left ventricular internal dimensions in dogs with and without cardiac disease measured from two-dimensional right parasternal short-axis images are interchangeable with those measured from M-mode images using the same view.
MeSH term(s)	Animals ; Dog Diseases ; Dogs ; Echocardiography/veterinary ; Heart Diseases/diagnostic imaging ; Heart Diseases/veterinary ; Heart Valve Diseases/veterinary ; Heart Ventricles/diagnostic imaging ; Mitral Valve ; Retrospective Studies
Language	English
Publishing date	2020-12-24
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2198278-8
ISSN	1875-0834 ; 1760-2734
ISSN (online)	1875-0834
ISSN	1760-2734
DOI	10.1016/j.jvc.2020.12.001
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Article: Seven year update of an EORTC phase III trial of high-dose intensity M-VAC chemotherapy and G-CSF versus classic M-VAC in advanced urothelial tract tumours.

Sternberg, C N / de Mulder, P / Schornagel, J H / Theodore, C / Fossa, S D / van Oosterom, A T / Witjes, J A / Spina, M / van Groeningen, C J / Duclos, B / Roberts, J T / de Balincourt, C / Collette, L

European journal of cancer (Oxford, England : 1990)

2006 Volume 42, Issue 1, Page(s) 50–54

Abstract: ... regimen of high-dose intensity chemotherapy with M-VAC plus granulocyte colony stimulating factor (HD-M ... VAC) compared to classic M-VAC in advanced transitional cell carcinoma (TCC). Two hundred and sixty ... response rate (RR) of 64% on the HD-M-VAC arm. On M-VAC, there were 12 CR (9%) and 53 PR (41 ...

Abstract	EORTC protocol 30924 is an international randomized trial reporting a 7.3 year update of a 2 weekly regimen of high-dose intensity chemotherapy with M-VAC plus granulocyte colony stimulating factor (HD-M-VAC) compared to classic M-VAC in advanced transitional cell carcinoma (TCC). Two hundred and sixty three untreated patients with bidimensionally measurable TCC were included. In an intention to treat analysis, there were 28 complete responses (CR) (21%) and 55 partial responses (PR) (41%), for an overall response rate (RR) of 64% on the HD-M-VAC arm. On M-VAC, there were 12 CR (9%) and 53 PR (41%), for an overall RR of 50% . The P-value for the difference in CR was 0.009; and for RR, was 0.06. After a median follow-up of 7.3 years, 24.6% are alive on the HD-M-VAC arm vs. 13.2% on the M-VAC arm. Median progression-free survival was better with HD-MVAC (9.5 months) vs. M-VAC (8.1 months). The mortality hazard ratio (HR) was 0.76. The 2-year survival rate for HD-M-VAC was 36.7% vs. 26.2% for M-VAC. At 5 years, the survival rate was 21.8% in the HD-M-VAC vs. 13.5%. Median survival was 15.1 months on HD-MVAC and 14.9 months on M-VAC. There was one death from toxicity in each arm; and more patients died to malignant disease in the M-VAC arm (76%) than in the HD-M-VAC arm (64.9%). With longer follow-up initial results have been confirmed, and shows that HD-M-VAC produces a borderline statistically significant relative reduction in the risk of progression and death compared to M-VAC.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Antineoplastic Combined Chemotherapy Protocols/administration & dosage ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Carcinoma, Transitional Cell/drug therapy ; Cisplatin/administration & dosage ; Doxorubicin/administration & dosage ; Female ; Follow-Up Studies ; Granulocyte Colony-Stimulating Factor/administration & dosage ; Humans ; Male ; Methotrexate/administration & dosage ; Middle Aged ; Survival Analysis ; Urologic Neoplasms/drug therapy ; Vinblastine/administration & dosage
Chemical Substances	Granulocyte Colony-Stimulating Factor (143011-72-7) ; Vinblastine (5V9KLZ54CY) ; Doxorubicin (80168379AG) ; Cisplatin (Q20Q21Q62J) ; Methotrexate (YL5FZ2Y5U1)
Language	English
Publishing date	2006-01
Publishing country	England
Document type	Clinical Trial, Phase III ; Journal Article ; Randomized Controlled Trial ; Research Support, N.I.H., Extramural
ZDB-ID	82061-1
ISSN	1879-0852 ; 0959-8049 ; 0277-5379 ; 0964-1947
ISSN (online)	1879-0852
ISSN	0959-8049 ; 0277-5379 ; 0964-1947
DOI	10.1016/j.ejca.2005.08.032
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Article ; Online: BRIT1/MCPH1 expression in chronic myeloid leukemia and its regulation of the G2/M checkpoint.

Giallongo, C / Tibullo, D / La Cava, P / Branca, A / Parrinello, N / Spina, P / Stagno, F / Conticello, C / Chiarenza, A / Vigneri, P / Palumbo, G A / Di Raimondo, F

Acta haematologica

2011 Volume 126, Issue 4, Page(s) 205–210

Abstract: ... of both the intra-S and G2/M checkpoints. The most important role of BRIT1/MCPH1 in the regulation of cell cycle ... progression appears to be the G2/M checkpoint. The K562 and peripheral blood cells of chronic myeloid leukemia ... of the G2/M cell cycle checkpoint arrest or of the apoptotic mechanism. This behavior may predispose ...

Abstract	BRIT1 (BRCT-repeat inhibitor of hTERT expression), also known as microcephalin (MCPH1), is a crucial gene in the complex cellular machine that is devoted to DNA repair and acts as a regulator of both the intra-S and G2/M checkpoints. The most important role of BRIT1/MCPH1 in the regulation of cell cycle progression appears to be the G2/M checkpoint. The K562 and peripheral blood cells of chronic myeloid leukemia (CML) patients at diagnosis were found to downregulate BRIT1/MCPH1. However, we could not find any correlation between bcr/abl activity and the BRIT1/MCPH1 level. In order to study the genomic instability of CML cells, we evaluated the ability of these cells to arrest mitotic division after exposure to hydroxyurea, a known genotoxic agent. We showed that CML cells continue to proliferate without the activation of the G2/M cell cycle checkpoint arrest or of the apoptotic mechanism. This behavior may predispose the cells to accumulate genomic defects. In conclusion, we found that CML cells have a low BRIT1/MCPH1 level and show a defective G2/M arrest, confirming that these cells have a constitutive genomic instability.
MeSH term(s)	Actins/antagonists & inhibitors ; Adult ; Aged ; Cell Proliferation/drug effects ; Cells, Cultured ; Cytochalasin B/toxicity ; Cytokinesis/drug effects ; Female ; G2 Phase Cell Cycle Checkpoints/drug effects ; Gene Expression Regulation, Neoplastic ; Genomic Instability ; Humans ; Hydroxyurea/antagonists & inhibitors ; Hydroxyurea/toxicity ; K562 Cells ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/blood ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/metabolism ; Leukocytes/drug effects ; Leukocytes/metabolism ; Male ; Middle Aged ; Mutagens/toxicity ; Neoplasm Proteins/genetics ; Neoplasm Proteins/metabolism ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/metabolism ; RNA, Messenger/metabolism
Chemical Substances	Actins ; MCPH1 protein, human ; Mutagens ; Neoplasm Proteins ; Nerve Tissue Proteins ; RNA, Messenger ; Cytochalasin B (3CHI920QS7) ; Hydroxyurea (X6Q56QN5QC)
Language	English
Publishing date	2011
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	80008-9
ISSN	1421-9662 ; 0001-5792
ISSN (online)	1421-9662
ISSN	0001-5792
DOI	10.1159/000329911
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Article ; Online: Effect of change in a normative approach on the evaluation of surface scratches on high-precision optical surfaces.

Špína, Michal / Procháska, František

Applied optics

2022 Volume 61, Issue 7, Page(s) 1593–1599

Abstract: The evaluation of optical surface defects is an integral part of the realization of precise optical elements. In addition to the traditionally used visual inspection procedures based on MIL-O-13830A, procedures based on ISO standards, which underwent ... ...

Abstract	The evaluation of optical surface defects is an integral part of the realization of precise optical elements. In addition to the traditionally used visual inspection procedures based on MIL-O-13830A, procedures based on ISO standards, which underwent some changes in 2017, are coming to the forefront. One of these changes was the omission of equivalent radiometric sizes and their replacement by geometric dimensions. This paper focuses just on the analysis of the impact of this change on the values of specifications defining the requirements on the scratch and dig quality of optical elements. Among other things, this presents a possible way to correct specification classes based on the statistical processing of a set of parameters of imperfections detected on optical element surfaces, which have been realized in the TOPTEC Centre.
Language	English
Publishing date	2022-03-14
Publishing country	United States
Document type	Journal Article
ISSN	1539-4522
ISSN (online)	1539-4522
DOI	10.1364/AO.445214
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Article: Antigen-induced bronchial hyperresponsiveness in the rabbit is not dependent on M(2)-receptor dysfunction.

Patel, H J / Douglas, G J / Herd, C M / Spina, D / Giembycz, M A / Barnes, P J / Belvisi, M G / Page, C P

Pulmonary pharmacology & therapeutics

1999 Volume 12, Issue 4, Page(s) 245–255

Abstract: ... the increased release in ACh was not associated with an alteration in M(2)-receptor function. Thus, antigen ... induced bronchial hyperresponsiveness in the rabbit does not appear to depend upon M(2)-receptor ...

Abstract	We have assessed the effect of sensitization to allergen on airway smooth muscle responsiveness and acetylcholine (ACh) release from cholinergic nerves in tracheal preparations from rabbits immunized at birth to Alternaria tenuis and littermate control rabbits injected with saline. ACh release induced by EFS was significantly greater in tracheal preparations obtained from immunized rabbits compared with littermate controls. The ability of the muscarinic-receptor agonist, oxotremorine, to inhibit ACh release to EFS (4 Hz) was not altered by immunization. The contractile response evoked by electrical field stimulation (EFS), ACh and 5-hydroxytryptamine (5-HT) was not significantly altered in tracheal preparations from antigen immunized rabbits compared with littermate controls. Antigen challenge of immunized rabbits did not affect the release of ACh from isolated trachea following EFS, or the ability of oxotremorine to inhibit ACh release. Furthermore, antigen challenge of immunized rabbits failed to alter the contractile response to EFS or ACh, but reduced the contractile potency of 5-HT. These results demonstrate increased ACh release in tracheal preparations following immunization which had no functional consequence on airway smooth muscle responsiveness. Moreover, the increased release in ACh was not associated with an alteration in M(2)-receptor function. Thus, antigen-induced bronchial hyperresponsiveness in the rabbit does not appear to depend upon M(2)-receptor dysfunction.
MeSH term(s)	Acetylcholine/pharmacology ; Acetylcholine/secretion ; Allergens/immunology ; Animals ; Bronchial Hyperreactivity/immunology ; Cholinergic Fibers/physiology ; Disease Models, Animal ; Female ; Hypersensitivity/immunology ; Male ; Muscle, Smooth/immunology ; Rabbits ; Receptors, Muscarinic/physiology
Chemical Substances	Allergens ; Receptors, Muscarinic ; Acetylcholine (N9YNS0M02X)
Language	English
Publishing date	1999
Publishing country	England
Document type	Journal Article
ZDB-ID	1399707-5
ISSN	1522-9629 ; 1094-5539
ISSN (online)	1522-9629
ISSN	1094-5539
DOI	10.1006/pupt.1999.0204
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Article: BRIT1/MCPH1 Expression in Chronic Myeloid Leukemia and Its Regulation of the G2/M Checkpoint

Giallongo, C. / Tibullo, D. / La Cava, P. / Branca, A. / Parrinello, N. / Spina, P. / Stagno, F. / Conticello, C. / Chiarenza, A. / Vigneri, P. / Palumbo, G.A. / Di Raimondo, F.

Acta Haematologica

2011 Volume 126, Issue 4, Page(s) 205–210

Institution	Department of Clinical and Molecular Biomedicine Section of Hematology, Ferrarotto Hospital and Section of Pathology, University of Catania, Catania, Italy
Abstract	BRIT1 (BRCT-repeat inhibitor of hTERT expression), also known as microcephalin (MCPH1), is a crucial gene in the complex cellular machine that is devoted to DNA repair and acts as a regulator of both the intra-S and G2/M checkpoints. The most important role of BRIT1/MCPH1 in the regulation of cell cycle progression appears to be the G2/M checkpoint. The K562 and peripheral blood cells of chronic myeloid leukemia (CML) patients at diagnosis were found to downregulate BRIT1/MCPH1. However, we could not find any correlation between bcr/abl activity and the BRIT1/MCPH1 level. In order to study the genomic instability of CML cells, we evaluated the ability of these cells to arrest mitotic division after exposure to hydroxyurea, a known genotoxic agent. We showed that CML cells continue to proliferate without the activation of the G2/M cell cycle checkpoint arrest or of the apoptotic mechanism. This behavior may predispose the cells to accumulate genomic defects. In conclusion, we found that CML cells have a low BRIT1/MCPH1 level and show a defective G2/M arrest, confirming that these cells have a constitutive genomic instability.
Keywords	Chronic myeloid leukemia ; DNA repair ; Genomic instability ; Microcephalin
Language	English
Publishing date	2011-09-16
Publisher	S. Karger AG
Publishing place	Basel, Switzerland
Document type	Article
Note	Original Paper
ZDB-ID	80008-9
ISSN	1421-9662 ; 0001-5792
ISSN (online)	1421-9662
ISSN	0001-5792
DOI	10.1159/000329911
Database	Karger publisher's database

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Article ; Online: Calcified uterine leiomyoma from an 18th-century nunnery in North Italy.

Fusco, Roberta / Tesi, Chiara / Spina, Paolo / Fulcheri, Ezio / Licata, Marta

International journal of paleopathology

2024 Volume 45, Page(s) 1–6

Abstract: Objective: To develop a differential diagnosis of a mass retrieved alongside skeletal remains in the crypt of the church of Santissima Annunziata of Valenza (Province of Alessandria, Northern Italy).: Material: A calcified mass measuring 40 × 39 mm ... ...

Abstract	Objective: To develop a differential diagnosis of a mass retrieved alongside skeletal remains in the crypt of the church of Santissima Annunziata of Valenza (Province of Alessandria, Northern Italy). Material: A calcified mass measuring 40 × 39 mm and 17.62 × 16.3817.62 × 16.38 mm. Method: The analysis utilized macroscopic assessment and histologic examination (including histochemical and immunohistochemical analyses). Results: Morphological traits include an irregular and spongy external surface. Holes of different sizes lead toward the inner part of the object. A section of the mass shows an "intertwined bundle" pattern, confirmed by microscopic examination. Conclusions: Differential diagnosis determined the mass to be consistent with calcified leiomyoma. Significance: Identifying uterine leiomyoma adds to the paucity of paleopathological literature on the condition and to calcified tumors more broadly. It also allows for an important discussion of women's gynecological health in the past and potentially among nulliparous women. Limitations: Neither histochemical staining nor immunohistochemical analysis demonstrated the certain muscular nature of the specimens due to the rehydration and decalcification processes, for which there are no gold standards. Suggestions for further research: Calcified masses are common in the clinical literature but remain rare in paleopathological literature. Careful excavation and improved recognition of apparently calcified masses are necessary to improve recognition, diagnosis, and interpretation.
Language	English
Publishing date	2024-02-19
Publishing country	Netherlands
Document type	Case Reports
ZDB-ID	2606330-X
ISSN	1879-9825 ; 1879-9817
ISSN (online)	1879-9825
ISSN	1879-9817
DOI	10.1016/j.ijpp.2024.01.005
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Article: The impact of culture on new Asian immigrants' access to oral health care: a scoping review.

Doucette, Heather / Yang, Sijie / Spina, Miriam

Canadian journal of dental hygiene : CJDH = Journal canadien de l'hygiene dentaire : JCHD

2023 Volume 57, Issue 1, Page(s) 33–43

Abstract: Background: Immigration has accounted for three-quarters of Canada's population growth since 2016, more than half of which has been from Asian countries. Newcomers from Asia have been reported to experience oral health disparities. The objective of this ...

Abstract	Background: Immigration has accounted for three-quarters of Canada's population growth since 2016, more than half of which has been from Asian countries. Newcomers from Asia have been reported to experience oral health disparities. The objective of this scoping review was to examine the literature discussing how culture affects access to oral health care for new immigrants from Asia and to identify knowledge gaps. Methods: The review was conducted from December 2021 to April 2022 following the Arskey and O'Malley approach and PRISMA-ScR guideline. Five databases were searched using the search parameter "Asian+ AND Immigrant+ AND oral care+". Only peer-reviewed articles published in English between 2011 and 2021 were included. Results: The search strategy yielded 736 articles. Duplicates were removed, titles and abstracts were reviewed, and the full text of 69 articles examined, leaving 26 articles that met eligibility criteria: 18 quantitative studies, 4 qualitative studies, and 4 reviews. Discussion: Four themes were identified: language barriers, oral health care access and service utilization, oral health beliefs and behaviour, and immigrant children's oral health. Most new immigrants from Asia have limited English proficiency, are of low socioeconomic status, and have difficulty developing trusting relationships with care providers. Immigrant children's oral health is impacted by their parents' beliefs. Conclusion: More research is needed on cultural barriers to and facilitators of access to oral health care for newcomers from Asia to Canada to aid in the development and implementation of policies and to inform practice and curriculum.
MeSH term(s)	Child ; Humans ; Oral Health ; Health Services Accessibility ; Emigrants and Immigrants ; Emigration and Immigration ; Communication Barriers
Language	English
Publishing date	2023-02-01
Publishing country	Canada
Document type	Systematic Review ; Review ; Journal Article
ISSN	1712-171X
ISSN	1712-171X
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Article ; Online: Evaluation of the Ekomille CO2® device as an animal welfare suppression system for rodent pests management

Spina, Giuseppe / Boccamazzo, Matteo

2023

Keywords	Text ; abstract_or_summary ; ddc:630
Language	English
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Increased Recognition of Non-O157 Shiga Toxin-Producing Escherichia coli Infections in the United States During 2000-2010: Epidemiologic Features and Comparison with E. coli O157 Infections. L. H. Gould, R. K. Mody, K. L. Ong, P. Clogher, A. B. Cronquist, K. N. Garman, S. Lathrop, C. Medus, N. L. Spina, T. H. Webb, P. L. White, K. Wymore, R. E. Gierke, B. E. Mahon, and P. M. Griffin, for the Emerging Infections Program Foodnet Working Group

Gould, L. H. / Mody, R. K. / Ong, K. L. / Clogher, P. / Cronquist, A. B. / Garman, K. N. / Lathrop, S. / Medus, C. / Spina, N. L. / Webb, T. H. / White, P. L. / Wymore, K. / Gierke, R. E. / Mahon, B. E. / Griffin, P. M.

Foodborne pathogens and disease

2013 Volume 10, Issue 5, Page(s) 453

Language	English
Document type	Article
ZDB-ID	2148479-X
ISSN	1535-3141
Database	Current Contents Nutrition, Environment, Agriculture

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