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  1. Article ; Online: Standardization of SARS-CoV-2 Cycle Threshold Values: Multisite Investigation Evaluating Viral Quantitation across Multiple Commercial COVID-19 Detection Platforms.

    Gavina, Kenneth / Franco, Lauren C / Robinson, Christopher M / Hymas, Weston / Lei, Guang-Sheng / Sinclair, Will / Hall, Tara / Carlquist, John / Lavik, John-Paul / Emery, Christopher L / Heaton, Phillip R / Hillyard, David / Lopransi, Bert K / Relich, Ryan F

    Microbiology spectrum

    2023  Volume 11, Issue 1, Page(s) e0447022

    Abstract: The demand for testing during the coronavirus disease 2019 (COVID-19) pandemic has resulted in the production of several different commercial platforms and laboratory-developed assays for the detection of severe acute respiratory syndrome coronavirus 2 ( ... ...

    Abstract The demand for testing during the coronavirus disease 2019 (COVID-19) pandemic has resulted in the production of several different commercial platforms and laboratory-developed assays for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This has created several challenges, including, but not limited to, the standardization of diagnostic testing, utilization of cycle threshold (
    MeSH term(s) Humans ; United States ; COVID-19/diagnosis ; SARS-CoV-2/genetics ; COVID-19 Testing ; Pandemics ; Clinical Laboratory Techniques/methods ; Reference Standards
    Language English
    Publishing date 2023-01-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.04470-22
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  2. Article ; Online: Association of Inadequate Provider Networks with Unmet Need for Health Services and Self-Employment among People with Disabilities.

    Hughes, Phillip M / Wu, Benjamin S / Annis, Izabela E / Brunelli, Caterina / Kurth, Noelle K / Hall, Jean P / Thomas, Kathleen C

    Journal of health care for the poor and underserved

    2022  Volume 33, Issue 2, Page(s) 984–997

    Abstract: People with disabilities (PWD) make up over a quarter of the U.S. population and often have complex medical needs. Insurance plans with narrow provider networks are growing in popularity despite concerns about limiting access to care, which may ... ...

    Abstract People with disabilities (PWD) make up over a quarter of the U.S. population and often have complex medical needs. Insurance plans with narrow provider networks are growing in popularity despite concerns about limiting access to care, which may detrimentally affect PWD. This study used logistic regression to assess the relationship between inadequate networks and unmet health care needs and employment using the 2018 National Survey on Health and Disability (n= 1,009) adjusting for demographic and health factors. Having an inadequate network was associated with unmet needs (OR=5.56, 95%CI[3.33,9.28]) but not being employed for wages (OR=0.70, 95%CI[0.42,1.17]) or self-employed (OR=2.35, 95%CI[0.99,5.55]). There was an association between an inadequate network and selfemployment for those with good health (OR=3.37, 95%CI[1.19,9.57]). Providers for PWD should be aware of the role insurance quality can play in health outcomes. Policymakers should continue to monitor the impact of provider network adequacy on health outcomes.
    MeSH term(s) Delivery of Health Care ; Disabled Persons ; Employment ; Health Services ; Health Services Accessibility ; Health Services Needs and Demand ; Humans
    Language English
    Publishing date 2022-05-27
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1142637-8
    ISSN 1548-6869 ; 1049-2089
    ISSN (online) 1548-6869
    ISSN 1049-2089
    DOI 10.1353/hpu.2022.0076
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    Zs.A 3605: Show issues Location:
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    Zs.MO 493: Show issues

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  3. Article ; Online: Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB.

    Jouravleva, Karina / Golovenko, Dmitrij / Demo, Gabriel / Dutcher, Robert C / Hall, Traci M Tanaka / Zamore, Phillip D / Korostelev, Andrei A

    Molecular cell

    2022  Volume 82, Issue 21, Page(s) 4049–4063.e6

    Abstract: In animals and plants, Dicer enzymes collaborate with double-stranded RNA-binding domain (dsRBD) proteins to convert precursor-microRNAs (pre-miRNAs) into miRNA duplexes. We report six cryo-EM structures of Drosophila Dicer-1 that show how Dicer-1 and ... ...

    Abstract In animals and plants, Dicer enzymes collaborate with double-stranded RNA-binding domain (dsRBD) proteins to convert precursor-microRNAs (pre-miRNAs) into miRNA duplexes. We report six cryo-EM structures of Drosophila Dicer-1 that show how Dicer-1 and its partner Loqs‑PB cooperate (1) before binding pre-miRNA, (2) after binding and in a catalytically competent state, (3) after nicking one arm of the pre-miRNA, and (4) following complete dicing and initial product release. Our reconstructions suggest that pre-miRNA binds a rare, open conformation of the Dicer‑1⋅Loqs‑PB heterodimer. The Dicer-1 dsRBD and three Loqs‑PB dsRBDs form a tight belt around the pre-miRNA, distorting the RNA helix to place the scissile phosphodiester bonds in the RNase III active sites. Pre-miRNA cleavage shifts the dsRBDs and partially closes Dicer-1, which may promote product release. Our data suggest a model for how the Dicer‑1⋅Loqs‑PB complex affects a complete cycle of pre-miRNA recognition, stepwise endonuclease cleavage, and product release.
    MeSH term(s) Animals ; Ribonuclease III/genetics ; Ribonuclease III/metabolism ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; RNA-Binding Proteins/metabolism ; Drosophila/genetics ; MicroRNAs/genetics ; MicroRNAs/metabolism
    Chemical Substances Ribonuclease III (EC 3.1.26.3) ; Drosophila Proteins ; RNA-Binding Proteins ; MicroRNAs
    Language English
    Publishing date 2022-09-30
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Intramural ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1415236-8
    ISSN 1097-4164 ; 1097-2765
    ISSN (online) 1097-4164
    ISSN 1097-2765
    DOI 10.1016/j.molcel.2022.09.002
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    Zs.A 5055: Show issues Location:
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  4. Article ; Online: An open label, non-randomized study assessing a prebiotic fiber intervention in a small cohort of Parkinson's disease participants.

    Hall, Deborah A / Voigt, Robin M / Cantu-Jungles, Thaisa M / Hamaker, Bruce / Engen, Phillip A / Shaikh, Maliha / Raeisi, Shohreh / Green, Stefan J / Naqib, Ankur / Forsyth, Christopher B / Chen, Tingting / Manfready, Richard / Ouyang, Bichun / Rasmussen, Heather E / Sedghi, Shahriar / Goetz, Christopher G / Keshavarzian, Ali

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 926

    Abstract: A pro-inflammatory intestinal microbiome is characteristic of Parkinson's disease (PD). Prebiotic fibers change the microbiome and this study sought to understand the utility of prebiotic fibers for use in PD patients. The first experiments demonstrate ... ...

    Abstract A pro-inflammatory intestinal microbiome is characteristic of Parkinson's disease (PD). Prebiotic fibers change the microbiome and this study sought to understand the utility of prebiotic fibers for use in PD patients. The first experiments demonstrate that fermentation of PD patient stool with prebiotic fibers increased the production of beneficial metabolites (short chain fatty acids, SCFA) and changed the microbiota demonstrating the capacity of PD microbiota to respond favorably to prebiotics. Subsequently, an open-label, non-randomized study was conducted in newly diagnosed, non-medicated (n = 10) and treated PD participants (n = 10) wherein the impact of 10 days of prebiotic intervention was evaluated. Outcomes demonstrate that the prebiotic intervention was well tolerated (primary outcome) and safe (secondary outcome) in PD participants and was associated with beneficial biological changes in the microbiota, SCFA, inflammation, and neurofilament light chain. Exploratory analyses indicate effects on clinically relevant outcomes. This proof-of-concept study offers the scientific rationale for placebo-controlled trials using prebiotic fibers in PD patients. ClinicalTrials.gov Identifier: NCT04512599.
    MeSH term(s) Humans ; Prebiotics ; Parkinson Disease ; Feces ; Fatty Acids, Volatile/metabolism ; Gastrointestinal Microbiome
    Chemical Substances Prebiotics ; Fatty Acids, Volatile
    Language English
    Publishing date 2023-02-18
    Publishing country England
    Document type Clinical Trial ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-36497-x
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  5. Article ; Online: Gut microbial metabolites in Parkinson's disease: Association with lifestyle, disease characteristics, and treatment status.

    Voigt, Robin M / Wang, Zeneng / Brown, J Mark / Engen, Phillip A / Naqib, Ankur / Goetz, Christopher G / Hall, Deborah A / Metman, Leo Verhagen / Shaikh, Maliha / Forsyth, Christopher B / Keshavarzian, Ali

    Neurobiology of disease

    2022  Volume 170, Page(s) 105780

    Abstract: There is growing appreciation of the importance of the intestinal microbiota in Parkinson's disease (PD), and one potential mechanism by which the intestinal microbiota can communicate with the brain is via bacteria-derived metabolites. In this study, ... ...

    Abstract There is growing appreciation of the importance of the intestinal microbiota in Parkinson's disease (PD), and one potential mechanism by which the intestinal microbiota can communicate with the brain is via bacteria-derived metabolites. In this study, plasma levels of bacterial-derived metabolites including trimethylamine-N-oxide (TMAO), short chain fatty acids (SCFA), the branched chain fatty acid isovalerate, succinate, and lactate were evaluated in PD subjects (treatment naïve and treated) which were compared to (1) population controls, (2) spousal / household controls (similar lifestyle to PD subjects), and (3) subjects with multiple system atrophy (MSA). Analyses revealed an increase in the TMAO pathway in PD subjects which was independent of medication status, disease characteristics, and lifestyle. Lactic acid was decreased in treated PD subjects, succinic acid positively correlated with disease severity, and the ratio of pro-inflammatory TMAO to the putative anti-inflammatory metabolite butyric acid was significantly higher in PD subjects compared to controls indicating a pro-inflammatory shift in the metabolite profile in PD subjects. Finally, acetic and butyric acid were different between PD and MSA subjects indicating that metabolites may differentiate these synucleinopathies. In summary, (1) TMAO is elevated in PD subjects, a phenomenon independent of disease characteristics, treatment status, and lifestyle and (2) metabolites may differentiate PD and MSA subjects. Additional studies to understand the potential of TMAO and other bacterial metabolites to serve as a biomarker or therapeutic targets are warranted.
    MeSH term(s) Bacteria ; Butyrates ; Gastrointestinal Microbiome ; Humans ; Life Style ; Multiple System Atrophy ; Parkinson Disease/therapy
    Chemical Substances Butyrates
    Language English
    Publishing date 2022-05-30
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 1211786-9
    ISSN 1095-953X ; 0969-9961
    ISSN (online) 1095-953X
    ISSN 0969-9961
    DOI 10.1016/j.nbd.2022.105780
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    Zs.A 4444: Show issues Location:
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  6. Article ; Online: Gut microbiome composition may be an indicator of preclinical Alzheimer's disease.

    Ferreiro, Aura L / Choi, JooHee / Ryou, Jian / Newcomer, Erin P / Thompson, Regina / Bollinger, Rebecca M / Hall-Moore, Carla / Ndao, I Malick / Sax, Laurie / Benzinger, Tammie L S / Stark, Susan L / Holtzman, David M / Fagan, Anne M / Schindler, Suzanne E / Cruchaga, Carlos / Butt, Omar H / Morris, John C / Tarr, Phillip I / Ances, Beau M /
    Dantas, Gautam

    Science translational medicine

    2023  Volume 15, Issue 700, Page(s) eabo2984

    Abstract: Alzheimer's disease (AD) pathology is thought to progress from normal cognition through preclinical disease and ultimately to symptomatic AD with cognitive impairment. Recent work suggests that the gut microbiome of symptomatic patients with AD has an ... ...

    Abstract Alzheimer's disease (AD) pathology is thought to progress from normal cognition through preclinical disease and ultimately to symptomatic AD with cognitive impairment. Recent work suggests that the gut microbiome of symptomatic patients with AD has an altered taxonomic composition compared with that of healthy, cognitively normal control individuals. However, knowledge about changes in the gut microbiome before the onset of symptomatic AD is limited. In this cross-sectional study that accounted for clinical covariates and dietary intake, we compared the taxonomic composition and gut microbial function in a cohort of 164 cognitively normal individuals, 49 of whom showed biomarker evidence of early preclinical AD. Gut microbial taxonomic profiles of individuals with preclinical AD were distinct from those of individuals without evidence of preclinical AD. The change in gut microbiome composition correlated with β-amyloid (Aβ) and tau pathological biomarkers but not with biomarkers of neurodegeneration, suggesting that the gut microbiome may change early in the disease process. We identified specific gut bacterial taxa associated with preclinical AD. Inclusion of these microbiome features improved the accuracy, sensitivity, and specificity of machine learning classifiers for predicting preclinical AD status when tested on a subset of the cohort (65 of the 164 participants). Gut microbiome correlates of preclinical AD neuropathology may improve our understanding of AD etiology and may help to identify gut-derived markers of AD risk.
    MeSH term(s) Humans ; Gastrointestinal Microbiome ; Alzheimer Disease ; Cross-Sectional Studies ; Microbiota ; Amyloid beta-Peptides
    Chemical Substances Amyloid beta-Peptides
    Language English
    Publishing date 2023-06-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 2518854-9
    ISSN 1946-6242 ; 1946-6234
    ISSN (online) 1946-6242
    ISSN 1946-6234
    DOI 10.1126/scitranslmed.abo2984
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    Zs.A 6941: Show issues Location:
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  7. Article ; Online: Factors that predict NCLEX-RN success at a Historically Black College and University: A four-year retrospective study.

    Banks, JoAnne / Onsomu, Elijah O / Hall, Susan / Hargis, Joanna / Ketner, Deborah H / Tyson, Teresa / Timcheck, Phillip M / Darby, Rita / Goodwin, Sheilia / McCullough, Elleton

    Journal of professional nursing : official journal of the American Association of Colleges of Nursing

    2022  Volume 39, Page(s) 177–186

    Abstract: Introduction: Ensuring NCLEX-RN success for graduates of Historically Black Colleges and Universities (HBCUs) is integral to increasing Blacks and other racial/ethnic minorities underrepresented as registered nurses (RNs).: Purpose: To examine the ... ...

    Abstract Introduction: Ensuring NCLEX-RN success for graduates of Historically Black Colleges and Universities (HBCUs) is integral to increasing Blacks and other racial/ethnic minorities underrepresented as registered nurses (RNs).
    Purpose: To examine the association between program type (traditional (TBSN) and accelerated Bachelor of Science [ABSN]) and first-time NCLEX-RN exam outcome.
    Methods: A retrospective descriptive design was used. TBSN and ABSN students (n = 465) who graduated between 2015-2018 at an HBCU were examined.
    Results: First-time pass rates for the total sample was 91.58%. Differences were observed between program type in 10 nursing courses for both programs, with Child Health being the only course predicting NCLEX-RN in both programs. In a multivariate logistic regression model, male students were 69% less likely to pass NCLEX-RN exam on first attempt compared to females, AOR = 0.31, p = 0.005.
    Conclusion: HBCUs can successfully prepare a diverse population of BSN graduates who pass NCLEX-RN on first attempt. Increased understanding of factors that decrease NCLEX-RN success of males is a prerequisite to development of strategies to ensure that all students have positive outcomes. There is also a need to understand differential relationships between nursing course grades and NCLEX-RN success among students enrolled in TBSN versus ABSN paths.
    MeSH term(s) Child ; Education, Nursing, Baccalaureate ; Educational Measurement ; Female ; Humans ; Licensure, Nursing ; Male ; Retrospective Studies ; Students, Nursing ; Universities
    Language English
    Publishing date 2022-02-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 632951-2
    ISSN 1532-8481 ; 8755-7223
    ISSN (online) 1532-8481
    ISSN 8755-7223
    DOI 10.1016/j.profnurs.2021.12.011
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    Zs.B 2985: Show issues Location:
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  8. Article ; Online: Nephrolithiasis: treatment, causes, and prevention.

    Hall, Phillip M

    Cleveland Clinic journal of medicine

    2009  Volume 76, Issue 10, Page(s) 583–591

    Abstract: Factors that promote stone formation include low daily urine volumes; saturation of the urine with calcium, oxalate, calcium phosphate, uric acid, or cystine; acidic urine; and bacterial infection. The author identifies the mechanisms of stone formation ... ...

    Abstract Factors that promote stone formation include low daily urine volumes; saturation of the urine with calcium, oxalate, calcium phosphate, uric acid, or cystine; acidic urine; and bacterial infection. The author identifies the mechanisms of stone formation and outlines management aimed at preventing recurrences.
    MeSH term(s) Adult ; Aged ; Calcium/analysis ; Female ; Humans ; Hypercalciuria/complications ; Hypercalciuria/prevention & control ; Hyperparathyroidism/complications ; Kidney Calculi/chemistry ; Male ; Middle Aged ; Nephrolithiasis/diagnosis ; Nephrolithiasis/etiology ; Nephrolithiasis/prevention & control ; Nephrolithiasis/therapy ; Secondary Prevention ; Uric Acid/analysis ; Young Adult
    Chemical Substances Uric Acid (268B43MJ25) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2009-10
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 639116-3
    ISSN 1939-2869 ; 0891-1150
    ISSN (online) 1939-2869
    ISSN 0891-1150
    DOI 10.3949/ccjm.76a.09043
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    Ua VI Zs.212: Show issues Location:
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  9. Article ; Online: Social and psychological adversity are associated with distinct mother and infant gut microbiome variations.

    Warner, Barbara B / Rosa, Bruce A / Ndao, I Malick / Tarr, Phillip I / Miller, J Philip / England, Sarah K / Luby, Joan L / Rogers, Cynthia E / Hall-Moore, Carla / Bryant, Renay E / Wang, Jacqueline D / Linneman, Laura A / Smyser, Tara A / Smyser, Christopher D / Barch, Deanna M / Miller, Gregory E / Chen, Edith / Martin, John / Mitreva, Makedonka

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 5824

    Abstract: Health disparities are driven by underlying social disadvantage and psychosocial stressors. However, how social disadvantage and psychosocial stressors lead to adverse health outcomes is unclear, particularly when exposure begins prenatally. Variations ... ...

    Abstract Health disparities are driven by underlying social disadvantage and psychosocial stressors. However, how social disadvantage and psychosocial stressors lead to adverse health outcomes is unclear, particularly when exposure begins prenatally. Variations in the gut microbiome and circulating proinflammatory cytokines offer potential mechanistic pathways. Here, we interrogate the gut microbiome of mother-child dyads to compare high-versus-low prenatal social disadvantage, psychosocial stressors and maternal circulating cytokine cohorts (prospective case-control study design using gut microbiomes from 121 dyads profiled with 16 S rRNA sequencing and 89 dyads with shotgun metagenomic sequencing). Gut microbiome characteristics significantly predictive of social disadvantage and psychosocial stressors in the mothers and children indicate that different discriminatory taxa and related pathways are involved, including many species of Bifidobacterium and related pathways across several comparisons. The lowest inter-individual gut microbiome similarity was observed among high-social disadvantage/high-psychosocial stressors mothers, suggesting distinct environmental exposures driving a diverging gut microbiome assembly compared to low-social disadvantage/low-psychosocial stressors controls (P = 3.5 × 10
    MeSH term(s) Female ; Pregnancy ; Humans ; Infant ; Gastrointestinal Microbiome/genetics ; Mothers ; Case-Control Studies ; Bifidobacterium/genetics ; Cytokines ; Vitamins
    Chemical Substances Cytokines ; Vitamins
    Language English
    Publishing date 2023-09-20
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-41421-4
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  10. Article: Twelve-Month Follow-up of Early COVID-19 Cases in the United States: Cellular and Humoral Immune Longevity.

    Shah, Melisa M / Rasheed, Mohammad Ata Ur / Harcourt, Jennifer L / Abedi, Glen R / Stumpf, Megan M / Kirking, Hannah L / Tamin, Azaibi / Mills, Lisa / Armstrong, Madeleine / Salvatore, Phillip P / Surasi, Krishna / Scott, Sarah E / Killerby, Marie E / Briggs-Hagen, Melissa / Saydah, Sharon / Tate, Jacqueline E / Fry, Alicia M / Hall, Aron J / Thornburg, Natalie J /
    Midgley, Claire M

    Open forum infectious diseases

    2022  Volume 9, Issue 3, Page(s) ofab664

    Abstract: We quantify antibody and memory B-cell responses to severe acute respiratory syndrome coronavirus 2 at 6 and 12 months postinfection among 7 unvaccinated US coronavirus disease 2019 cases. All had detectable S-specific memory B cells and immunoglobulin G ...

    Abstract We quantify antibody and memory B-cell responses to severe acute respiratory syndrome coronavirus 2 at 6 and 12 months postinfection among 7 unvaccinated US coronavirus disease 2019 cases. All had detectable S-specific memory B cells and immunoglobulin G at both time points, with geometric mean titers of 117.2 BAU/mL and 84.0 BAU/mL at 6 and 12 months, respectively.
    Language English
    Publishing date 2022-01-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2757767-3
    ISSN 2328-8957
    ISSN 2328-8957
    DOI 10.1093/ofid/ofab664
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