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  1. Article ; Online: Segmental Unconventional Outflow in Mouse Eyes.

    Li, Hoi-Lam / Ren, Ruiyi / Gong, Haiyan

    Investigative ophthalmology & visual science

    2023  Volume 64, Issue 15, Page(s) 26

    Abstract: Purpose: To investigate the flow pattern in unconventional outflow and its correlation with conventional outflow in mouse eyes.: Methods: Fluorescent microspheres were injected into the anterior chamber of one eye of anesthetized C57BL/6J mice (n = 4) ...

    Abstract Purpose: To investigate the flow pattern in unconventional outflow and its correlation with conventional outflow in mouse eyes.
    Methods: Fluorescent microspheres were injected into the anterior chamber of one eye of anesthetized C57BL/6J mice (n = 4), followed by perfused fixation with 4% paraformaldehyde in situ after 45 minutes. Post-euthanasia, the injected eyes were enucleated, further immersion fixed, and dissected into 12 equal radial segments. Both sides of each segment were imaged using a confocal microscope after nuclear counterstaining. Both unconventional and conventional outflow patterns of each eye were analyzed by ImageJ and ZEN 2.3 imaging software.
    Results: Segmental outflow patterns were observed in both the ciliary body (CB) and the supraciliary space and suprachoroidal space (SCS). In the CB, the tracer intensity was the lowest at 12 o'clock and highest at 9 o'clock, whereas in the SCS it was the lowest at 2 o'clock and the highest at 10 o'clock. Consequently, a segmental unconventional outflow was observed, with the lowest and highest flow regions in the superior and temporal quadrants, respectively. The overall segmental uveoscleral outflow has no correlation with trabecular outflow (P > 0.05). Four different outflow patterns were observed: (1) low-flow regions in both outflows, (2) primarily a high-flow region in conventional outflow, (3) primarily a high-flow region in unconventional outflow, and (4) high-flow regions in both outflows.
    Conclusions: Uveoscleral outflow is segmental and unrelated to the trabecular segmental outflow. These findings will lead to future studies to identify the best location for the placement of drainage devices and drug delivery.
    MeSH term(s) Mice ; Animals ; Mice, Inbred C57BL ; Ciliary Body ; Anterior Chamber ; Coloring Agents ; Drainage
    Chemical Substances Coloring Agents
    Language English
    Publishing date 2023-12-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 391794-0
    ISSN 1552-5783 ; 0146-0404
    ISSN (online) 1552-5783
    ISSN 0146-0404
    DOI 10.1167/iovs.64.15.26
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The oral administration of

    Lv, Longxian / Ren, Siqi / Jiang, Huiyong / Yan, Ren / Chen, Wenyi / Yan, Ruiyi / Dong, Jinming / Shao, Li / Yu, Ying

    mSphere

    2024  Volume 9, Issue 1, Page(s) e0067223

    Abstract: Acetaminophen is a widely used antipyretic and analgesic drug, and its overdose is the leading cause of drug-induced acute liver failure. This study aimed to investigate the effect and mechanism ... ...

    Abstract Acetaminophen is a widely used antipyretic and analgesic drug, and its overdose is the leading cause of drug-induced acute liver failure. This study aimed to investigate the effect and mechanism of
    MeSH term(s) Humans ; Mice ; Animals ; Acetaminophen/adverse effects ; Lacticaseibacillus casei ; Antipyretics ; Chemical and Drug Induced Liver Injury, Chronic ; Proteomics ; Mice, Inbred C57BL ; Administration, Oral ; Liver Failure ; Analgesics ; Glutathione ; Bilirubin
    Chemical Substances Acetaminophen (362O9ITL9D) ; Antipyretics ; Analgesics ; Glutathione (GAN16C9B8O) ; Bilirubin (RFM9X3LJ49)
    Language English
    Publishing date 2024-01-09
    Publishing country United States
    Document type Journal Article
    ISSN 2379-5042
    ISSN (online) 2379-5042
    DOI 10.1128/msphere.00672-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Rho Kinase Inhibitor AR-12286 Reverses Steroid-Induced Changes in Intraocular Pressure, Effective Filtration Areas, and Morphology in Mouse Eyes.

    Ren, Ruiyi / Humphrey, Anne A / Kopczynski, Casey / Gong, Haiyan

    Investigative ophthalmology & visual science

    2023  Volume 64, Issue 2, Page(s) 7

    Abstract: Purpose: We investigated mechanisms of reduction of intraocular pressure (IOP) by Rho kinase inhibitor AR-12286 in steroid-induced ocular hypertension (SIOH).: Methods: C57BL/6 mice (N = 56) were randomly divided into Saline, dexamethasone (DEX), DEX ...

    Abstract Purpose: We investigated mechanisms of reduction of intraocular pressure (IOP) by Rho kinase inhibitor AR-12286 in steroid-induced ocular hypertension (SIOH).
    Methods: C57BL/6 mice (N = 56) were randomly divided into Saline, dexamethasone (DEX), DEX + AR-12286, and DEX-discontinuation (DEX-DC) groups. IOP was measured weekly during the first four weeks in all groups. Beginning at week 5, the DEX-DC group was followed without treatment until IOP returned to normal, and the other groups were treated as assigned with IOP measured every other day for another week. Fluorescent tracer was injected into the anterior chamber to visualize the outflow pattern in the trabecular meshwork (TM) and TM effective filtration area (EFA) was determined. Radial sections from both high- and low-tracer regions were processed for electron microscopy.
    Results: AR-12286 reduced IOP in SIOH mouse eyes in one day (P < 0.01). At the end of week 5, mean IOP in the DEX + AR-12286 group was ∼4 mm Hg lower than DEX group (P < 0.001) and ∼2 mm Hg lower than DEX-DC group (P < 0.05). After one-week AR-12286 treatment (P < 0.05) or five-week DC of DEX (P < 0.01), DEX-induced reduction of EFA was rescued and DEX-induced morphological changes in the TM were partially reversed.
    Conclusions: AR-12286 reversed steroid-induced morphological changes in the TM and reduced EFA, which correlated with reduced IOP in SIOH eyes. AR-12286 reduced IOP elevation in SIOH eyes more effectively than discontinuing DEX treatment even when accompanied by continuous DEX treatment. Therefore Rho kinase inhibitors may lower SIOH in patients who rely on steroid treatment.
    MeSH term(s) Animals ; Mice ; Glaucoma ; Intraocular Pressure ; Mice, Inbred C57BL ; Ocular Hypertension/chemically induced ; Ocular Hypertension/drug therapy ; rho-Associated Kinases/pharmacology ; Trabecular Meshwork
    Chemical Substances AR-12286 ; rho-Associated Kinases (EC 2.7.11.1)
    Language English
    Publishing date 2023-02-01
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 391794-0
    ISSN 1552-5783 ; 0146-0404
    ISSN (online) 1552-5783
    ISSN 0146-0404
    DOI 10.1167/iovs.64.2.7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Healthy lifestyle decreases the risk of the first incidence of non-communicable chronic disease and its progression to multimorbidity and its mediating roles of metabolic components: a prospective cohort study in China.

    Lai, Chong / Fu, Ruiyi / Huang, Changzhen / Wang, Lu / Ren, Haiqing / Zhu, Yimin / Zhang, Xuhui

    The journal of nutrition, health & aging

    2024  Volume 28, Issue 3, Page(s) 100164

    Abstract: Objectives: To identify the influence of healthy lifestyles on the incidence of the first NCD (FNCD), multiple chronic conditions (MCCs), and the progression from FNCD to MCCs.: Design: cohort study.: Setting: Zhejiang, China PARTICIPANTS: 10566 ... ...

    Abstract Objectives: To identify the influence of healthy lifestyles on the incidence of the first NCD (FNCD), multiple chronic conditions (MCCs), and the progression from FNCD to MCCs.
    Design: cohort study.
    Setting: Zhejiang, China PARTICIPANTS: 10566 subjects (55.5 ± 13.5 years, 43.1% male) free of NCDs at baseline from the Zhejiang Metabolic Syndrome prospective cohort.
    Measurements: Healthy lifestyle score (HLS) was developed by 6 common healthy lifestyle factors as smoking, alcohol drinking, physical activity, body mass index (BMI) and waist-to-hip ratio (WHR). Healthy lifestyle data and metabolic biomarkers collected via a face-to-face questionnaire-based interview, clinical health examination and routine biochemical determination. Biochemical variables were determined using biochemical auto-analyzer. Participants were stratified into four group based on the levels of HLS as ≤2, 3, 4 and ≥5. Multiple Cox proportional hazards model was applied to examine the relationship between HLS and the risk of FNCD, MCCs and the progression from FNCD to MCCs. The population-attributable fractions (PAF) were used to assess the attributable role of HLS. Mediating effect was examined by mediation package in R.
    Results: After a median of 9.92 years of follow-up, 1572 participants (14.9%) developed FNCD, and 149 (1.4%) developed MCCs. In the fully adjusted model, the higher HLS group (≥5) was associated with lower risk of FNCD (HR = 0.68 and 95% CI: 0.56-0.82), MCCs (HR = 0.31 and 95%CI: 0.14-0.69); and the progression from FNCD to MCCs (HR = 0.39 and 95%CI: 0.18-0.85). Metabolic components (TC, TG, HDL-C, LDC-C, FPG, and UA) played the mediating roles with the proportion ranging from 5.02% to 22.2% for FNCD and 5.94% to 20.1% for MCCs. PAFs (95%CI) for poor adherence to the overall healthy lifestyle (HLS ≤ 3) were 17.5% (11.2%, 23.7%) for FNCD, 42.9% (23.4%, 61.0%) for MCCs, and 37.0% (15.5%, 56.3%) for the progression from FNCD to MCCs.
    Conclusions: High HLS decreases the risk of FNCD, MCCs, and the progression from FNCD to MCCs. These effects are partially mediated by metabolic components. Maintaining healthy lifestyles might reduce the disease burden of common chronic diseases.
    MeSH term(s) Humans ; Male ; Female ; Cohort Studies ; Prospective Studies ; Noncommunicable Diseases/epidemiology ; Noncommunicable Diseases/prevention & control ; Risk Factors ; Incidence ; Multimorbidity ; Healthy Lifestyle
    Language English
    Publishing date 2024-02-01
    Publishing country France
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2081921-3
    ISSN 1760-4788 ; 1279-7707
    ISSN (online) 1760-4788
    ISSN 1279-7707
    DOI 10.1016/j.jnha.2024.100164
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Relationships between Intraocular Pressure, Effective Filtration Area, and Morphological Changes in the Trabecular Meshwork of Steroid-Induced Ocular Hypertensive Mouse Eyes.

    Ren, Ruiyi / Humphrey, Anne A / Swain, David L / Gong, Haiyan

    International journal of molecular sciences

    2022  Volume 23, Issue 2

    Abstract: We investigated whether an inverse relationship exists between intraocular pressure (IOP) and effective filtration area (EFA) in the trabecular meshwork (TM) in a steroid-induced ocular hypertensive (SIOH) mouse model and the morphological changes ... ...

    Abstract We investigated whether an inverse relationship exists between intraocular pressure (IOP) and effective filtration area (EFA) in the trabecular meshwork (TM) in a steroid-induced ocular hypertensive (SIOH) mouse model and the morphological changes associated with the reduction of EFA. C57BL/6 mice (
    MeSH term(s) Animals ; Basement Membrane/metabolism ; Basement Membrane/pathology ; Basement Membrane/ultrastructure ; Biomarkers ; Body Weight/drug effects ; Dexamethasone/adverse effects ; Dexamethasone/pharmacology ; Dexamethasone/therapeutic use ; Disease Models, Animal ; Disease Susceptibility ; Fluorescent Antibody Technique ; Glaucoma/diagnosis ; Glaucoma/etiology ; Glaucoma/metabolism ; Intraocular Pressure/drug effects ; Mice ; Steroids/adverse effects ; Steroids/therapeutic use ; Trabecular Meshwork/metabolism ; Trabecular Meshwork/pathology ; Trabecular Meshwork/ultrastructure
    Chemical Substances Biomarkers ; Steroids ; Dexamethasone (7S5I7G3JQL)
    Language English
    Publishing date 2022-01-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23020854
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  6. Article: Effect of semaglutide and empagliflozin on cognitive function and hippocampal phosphoproteomic in obese mice.

    Chen, Xiaoyi / Chen, Shuchun / Li, Zelin / Zhu, Ruiyi / Jia, Zhuoya / Ban, Jiangli / Zhen, Ruoxi / Chen, Xing / Pan, Xiaoyu / Ren, Qingjuan / Yue, Lin / Niu, Shu

    Frontiers in pharmacology

    2023  Volume 14, Page(s) 975830

    Abstract: Objective: ...

    Abstract Objective:
    Language English
    Publishing date 2023-03-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2023.975830
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  7. Article ; Online: Relationships between Intraocular Pressure, Effective Filtration Area, and Morphological Changes in the Trabecular Meshwork of Steroid-Induced Ocular Hypertensive Mouse Eyes

    Ruiyi Ren / Anne A. Humphrey / David L. Swain / Haiyan Gong

    International Journal of Molecular Sciences, Vol 23, Iss 854, p

    2022  Volume 854

    Abstract: We investigated whether an inverse relationship exists between intraocular pressure (IOP) and effective filtration area (EFA) in the trabecular meshwork (TM) in a steroid-induced ocular hypertensive (SIOH) mouse model and the morphological changes ... ...

    Abstract We investigated whether an inverse relationship exists between intraocular pressure (IOP) and effective filtration area (EFA) in the trabecular meshwork (TM) in a steroid-induced ocular hypertensive (SIOH) mouse model and the morphological changes associated with the reduction of EFA. C57BL/6 mice ( n = 15 per group) received either 0.1% dexamethasone (DEX) or saline eye drops twice daily for five weeks. IOP was measured weekly. Fluorescent tracers were injected into the anterior chamber to label EFA at the endpoint. Injected eyes were fixed and processed for confocal microscopy. EFA in the TM was analyzed. Light and electron microscopy were performed in high- and low-tracer regions of six eyes per group. The mean IOP was ~4 mm Hg higher in DEX-treated than saline-treated control eyes ( p < 0.001) at the endpoint. EFA was reduced in DEX-treated eyes compared to controls ( p < 0.01) and negatively correlated with IOP ( R 2 = 0.38, p = 0.002). Reduced thickness of juxtacanalicular tissue (JCT) and increased abnormal extracellular matrix in the JCT were found to be associated with reduced EFA. Our data confirm the inverse relationship between EFA and IOP, suggesting that morphological changes in the JCT contribute to the reduction of EFA, thus elevating IOP in SIOH mouse eyes.
    Keywords steroid-induced ocular hypertensive mouse model ; effective filtration area ; trabecular meshwork ; intraocular pressure ; morphology ; confocal microscopy ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 630
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Incidence and risk factors of early transient intraocular pressure elevation after canaloplasty for primary open-angle glaucoma.

    Xu, Lijuan / Zhang, Xinyao / Cao, Yang / Zhao, Yin / Gu, Juan / Ye, Wenqing / Wang, Xiaojie / Li, Jinxin / Ren, Ruiyi / Liang, Yuanbo

    Journal of Zhejiang University. Science. B

    2023  Volume 24, Issue 4, Page(s) 366–370

    Abstract: Glaucoma is one of the most common optic neuropathies, featuring progressive retinal ganglion cell damage and visual field loss (Tham et al., 2014; Xu et al., 2020). Currently, the only effective treatment for this condition is the reduction of ... ...

    Title translation 原发性开角型青光眼黏小管成形术后早期短暂性高眼压的发生率及危险因素分析.
    Abstract Glaucoma is one of the most common optic neuropathies, featuring progressive retinal ganglion cell damage and visual field loss (Tham et al., 2014; Xu et al., 2020). Currently, the only effective treatment for this condition is the reduction of intraocular pressure (IOP) (Palmberg, 2001; Heijl et al., 2002). Canaloplasty is a proven bleb-independent surgery with good efficacy and safety profiles in primary open-angle glaucoma (POAG) (Gołaszewska et al., 2021). However, early transient postoperative IOP elevation has been reported in up to 30% of cases (Riva et al., 2019), similar to that commonly observed in other internal drainage glaucoma surgeries such as implantation using iStent (0%-21.0%), CyPass (10.8%), and Hydrus (4.8%-6.5%) (Lavia et al., 2017). This complication may be a predictor of poor reserve in the outflow system and is potentially associated with surgical failure. Nonetheless, the exact pathophysiology of glaucoma remains unknown, and studies clarifying the risk factors for postoperative IOP elevation have been scarce.
    MeSH term(s) Humans ; Intraocular Pressure ; Glaucoma, Open-Angle/surgery ; Incidence ; Treatment Outcome ; Risk Factors
    Language Chinese
    Publishing date 2023-04-13
    Publishing country China
    Document type Journal Article
    ZDB-ID 2247290-3
    ISSN 1862-1783 ; 1673-1581
    ISSN (online) 1862-1783
    ISSN 1673-1581
    DOI 10.1631/jzus.B2200485
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Effect of high-fat diet and empagliflozin on cardiac proteins in mice

    Pan, Xiaoyu / Chen, Shuchun / Chen, Xing / Ren, Qingjuan / Yue, Lin / Niu, Shu / Li, Zelin / Zhu, Ruiyi / Chen, Xiaoyi / Jia, Zhuoya / Zhen, Ruoxi / Ban, Jiangli

    Nutr Metab (Lond). 2022 Dec., v. 19, no. 1 p.69-69

    2022  

    Abstract: Using proteomic techniques the impact of the sodium-glucose transport protein 2 inhibitor empagliflozin on cardiac protein expression in a mouse model was assessed under normal and high-fat diet (HFD) conditions. We examined the effect of obesity on ... ...

    Abstract Using proteomic techniques the impact of the sodium-glucose transport protein 2 inhibitor empagliflozin on cardiac protein expression in a mouse model was assessed under normal and high-fat diet (HFD) conditions. We examined the effect of obesity on serological markers and heart function in obese mice treated with or without empagliflozin and used proteomic techniques to investigate alterations in cardiac protein expression. Using bioinformatic techniques, data were screened for differentially expressed proteins (DEPs) implicated in the putative mechanism of empagliflozin's cardioprotective effects. In C57BL/6 mice, HFD increased body weight, blood lipid, and glucose levels and was associated with structural damage to the heart. Empagliflozin reduces body weight, improves glucose and lipid metabolism, alleviates obesity-induced cardiac ventricular wall thickening, and lowers cardiac tissue collagen. The expression of several proteins was altered in the heart, mainly related to lipid metabolism. Following empagliflozin treatment, the expression of several lipid metabolism-related proteins was considerably reduced. Further examination of DEPs revealed that following empagliflozin treatment, the expressions of Apoe, Apoc1, Saa2, Apoa2, and Pon1 altered dramatically, suggesting that these proteins may be the main proteins that empagliflozin uses to treat obesity-induced aberrant lipid metabolism. Empagliflozin may protect the heart by altering the expression of genes including Apoe, Apoc1, Saa2, Apoa2, and Pon1, which are all involved in lipid metabolism disturbance in obesity.
    Keywords bioinformatics ; blood lipids ; collagen ; gene expression regulation ; glucose ; heart ; high fat diet ; lipid metabolism ; lipids ; mice ; models ; obesity ; protein synthesis ; proteomics ; transport proteins
    Language English
    Dates of publication 2022-12
    Size p. 69.
    Publishing place BioMed Central
    Document type Article ; Online
    ZDB-ID 2160376-5
    ISSN 1743-7075
    ISSN 1743-7075
    DOI 10.1186/s12986-022-00705-0
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: A metabonomics-based renoprotective mechanism analysis of empagliflozin in obese mice

    Chen, Xing / Chen, Shuchun / Ren, Qingjuan / Niu, Shu / Yue, Lin / Pan, Xiaoyu / Li, Zelin / Zhu, Ruiyi / Jia, Zhuoya / Chen, Xiaoyi / Zhen, Ruoxi / Ban, Jiangli

    Biochemical and biophysical research communications. 2022 Sept. 17, v. 621

    2022  

    Abstract: With an increasing prevalence of obesity related kidney disease, exploring the mechanisms of therapeutic method is of critical importance. Empagliflozin is a new antidiabetic agent with broad clinical application prospect in cardiovascular and renal ... ...

    Abstract With an increasing prevalence of obesity related kidney disease, exploring the mechanisms of therapeutic method is of critical importance. Empagliflozin is a new antidiabetic agent with broad clinical application prospect in cardiovascular and renal diseases. However, a metabonomics-based renoprotective mechanism of empagliflozin in obesity remains unclear. Our results showed that empagliflozin significantly alleviated the deposition of lipid droplet, glomerular and tubular injury. The innovation lied in detection of empagliflozin-targeted differential metabolites in kidneys. Compared with normal control mice, obese mice showed higher levels of All-trans-heptaprenyl diphosphate, Biliverdin, Galabiose, Galabiosylceramide (d18:1/16:0), Inosine, Methylisocitric acid, Uric acid, Xanthosine, O-glutarylcarnitine, PG(20:3(8Z,11Z,14Z)/0:0), PG(20:4(5Z,8Z,11Z,14Z)/0:0), PE(O-16:0/0:0), PG(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/0:0), and lower level of Adenosine. Empagliflozin regulated these metabolites in the opposite direction. Associated metabolic pathways were Phospholipids metabolism, Purine metabolism, and Biliverdin metabolism. Most of metabolites were associated with inflammatory response and oxidative stress. Empagliflozin improved the oxidative stress and inflammation imbalance. Our study revealed the metabonomics-based renoprotective mechanism of empagliflozin in obese mice for the first time. Empagliflozin may be a promising tool to delay the progression of obesity-related kidney disease.
    Keywords adenosine ; droplets ; hypoglycemic agents ; inflammation ; inosine ; kidney diseases ; metabolites ; obesity ; oxidative stress ; phospholipids ; research ; therapeutics ; uric acid
    Language English
    Dates of publication 2022-0917
    Size p. 122-129.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 205723-2
    ISSN 0006-291X ; 0006-291X
    ISSN (online) 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2022.06.091
    Database NAL-Catalogue (AGRICOLA)

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