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  1. Article ; Online: Runs of Homozygosity Revealed Reproductive Traits of Hu Sheep.

    Li, Yuzhe / Chen, Zitao / Fang, Yifei / Cao, Caiyun / Zhang, Zhe / Pan, Yuchun / Wang, Qishan

    Genes

    2022  Volume 13, Issue 10

    Abstract: Hu sheep, a famous breed in the Taihu Basin, has the advantages of non-seasonal estrus, multiple ... of ROHs. Here, we sequenced 108 Hu sheep, detected ROHs in Hu sheep to calculate their inbreeding ... coefficient, and selected genes of Hu sheep breeds within the ROH islands which are relevant to agricultural ...

    Abstract Hu sheep, a famous breed in the Taihu Basin, has the advantages of non-seasonal estrus, multiple fetuses, coarse feeding tolerance, and suitability for house feeding. Runs of homozygosity (ROHs) were found to be an effective tool to detect the animal population structure and economic traits. The detection of ROHs is beneficial for reducing the incidence of inbreeding as well as identifying harmful variants in the genome. However, there is a lack of systemic reports on ruminants in previous studies of ROHs. Here, we sequenced 108 Hu sheep, detected ROHs in Hu sheep to calculate their inbreeding coefficient, and selected genes of Hu sheep breeds within the ROH islands which are relevant to agricultural economic characteristics. Then, we compared the characteristics of the occurrences of SNPs between Hu sheep and other sheep breeds, and also investigated the distribution of the frequencies of SNPs within specific gene regions of Hu sheep breeds to select their breed-specific genes. Furthermore, we performed a comparative genome and transcriptome analysis in human and sheep breeds to identify important reproduction-related genes. In this way, we found some significant SNPs, and mapped these with a set of interesting candidate genes which are related to the productive value of livestock (
    MeSH term(s) Female ; Humans ; Sheep/genetics ; Animals ; Ephrin-B3 ; Homozygote ; Inbreeding ; Reproduction/genetics ; Livestock
    Chemical Substances Ephrin-B3
    Language English
    Publishing date 2022-10-13
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2527218-4
    ISSN 2073-4425 ; 2073-4425
    ISSN (online) 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes13101848
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  2. Article ; Online: Supercritical Carbon Dioxide Extraction Process for Hibiscus taiwanensis S. Y. Hu and Tyrosinase Inhibitory Activity of Its Extract

    Jingguo Yang / Fawu Pan / Liyong Du

    Processes, Vol 10, Iss 2218, p

    2022  Volume 2218

    Abstract: In this study, the bioactive components in the stem of Hibiscus taiwanensis S. Y. Hu were extracted ... the optimal conditions, the extraction rate of Hibiscus taiwanensis S. Y. Hu reached more than 0.5% within 2 h ... In addition, the related activity of extract in the stem Hibiscus taiwanensis S. Y. Hu was evaluated ...

    Abstract In this study, the bioactive components in the stem of Hibiscus taiwanensis S. Y. Hu were extracted by supercritical carbon dioxide (SC-CO 2 ), and the inhibition activity of the extract tyrosinase was analyzed. When the particle size of the powder was controlled to be 50 mesh, the effects of temperatures (40, 45, 50, 55, and 60 °C), pressures (15, 20, 25, 30, and 35 MPa), concentrations of entrainment agent (ethanol) (60, 80, and 95%) and CO 2 flow rates (80, 100, 120, and 140 L/min) on the extraction rate, respectively, were studied with the single-variable method. The optimal parameters included an extraction temperature of 55 °C, an extraction pressure of 30 MPa, 80% ethanol as the entrainment agent, and the CO 2 flow rate of 100 L/min. Under the optimal conditions, the extraction rate of Hibiscus taiwanensis S. Y. Hu reached more than 0.5% within 2 h. At the same time, the chemical compositions of the extract were investigated by using high-resolution liquid chromatography-mass spectrometry (LC-MS) equipped with a linear ion trap-electrostatic field orbital trap. In addition, the related activity of extract in the stem Hibiscus taiwanensis S. Y. Hu was evaluated by tyrosinase inhibition experiments. The results showed that the inhibition rate of the extract with a concentration of 0.25 mg/mL to tyrosinase reached 35.60%, indicating that the extract by SC-CO 2 had the potential to be used for whitening active raw materials.
    Keywords supercritical carbon dioxide extraction ; Hibiscus taiwanensis S. Y. Hu ; whitening ; tyrosinase inhibitory activity ; Chemical technology ; TP1-1185 ; Chemistry ; QD1-999
    Subject code 660
    Language English
    Publishing date 2022-10-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Building Interior Layout Design Based on Building Information Model and Deep Learning Technology: Taking the Interior Renewal Design of the Fifth Floor of the Procuratorate of Dong Xi Hu District as an Example.

    Pan, Haowei / Zheng, Gewei / Hutter, Ákos / Huang, Zhenkai

    publication RETRACTED

    Computational intelligence and neuroscience

    2022  Volume 2022, Page(s) 3746393

    Abstract: With the development of the Internet era and the information age, electronic information technology has profoundly affected human life and work, and the architectural design industry is no exception. Architectural design has a complex design period, and ... ...

    Abstract With the development of the Internet era and the information age, electronic information technology has profoundly affected human life and work, and the architectural design industry is no exception. Architectural design has a complex design period, and the arbitrary division of labour reflects the particularity of its working process. At present, the integration of electronic information technology and scientific and technological software into architectural design has dramatically improved the work efficiency of designers. However, due to the enormous workload of drawing and modification of building drawings, and many professional departments involved in cooperation, the error rate of drawing design is still high, which eventually leads to a series of serious problems such as stagnation of the design process and rework of construction. However, due to the enormous workload of drawing and modification of building drawings, and many professional departments involved in cooperation, the error rate of drawing design is still high, which eventually leads to a series of serious problems such as stagnation of the design process and rework of construction. The BIM building information model system is an electronic information platform for comprehensive architectural design majors. It can integrate the design drawings of various majors in the design process, correct the design problems caused by inaccurate pictures at the first time, and reduce the errors in the design process. Through the BIM system and Python programming software, cross-platform cooperation is carried out to carry out computer deep learning and a series of extra design work. This paper puts forward the interior design method of the BIM system and shows the research work of interior design by the BIM building information platform through practical cases. By comparing with traditional design methods, the advantages of this technology in interior design are demonstrated, and a reference for future interior design informatization is provided.
    MeSH term(s) Humans ; Interior Design and Furnishings ; Deep Learning ; Software ; Technology
    Language English
    Publishing date 2022-10-10
    Publishing country United States
    Document type Journal Article ; Retracted Publication
    ZDB-ID 2388208-6
    ISSN 1687-5273 ; 1687-5273
    ISSN (online) 1687-5273
    ISSN 1687-5273
    DOI 10.1155/2022/3746393
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  4. Article ; Online: The impact of xylem cavitation on water potential isotherms measured by the pressure chamber technique in Metasequoia glyptostroboides Hu & W.C. Cheng.

    Yang, Dongmei / Pan, Shaoan / Tyree, Melvin T

    Journal of experimental botany

    2016  Volume 67, Issue 15, Page(s) 4571–4580

    Abstract: Pressure-volume (PV) curve analysis is the most common and accurate way of estimating all components of the water relationships in leaves (water potential isotherms) as summarized in the Höfler diagram. PV curve analysis yields values of osmotic pressure, ...

    Abstract Pressure-volume (PV) curve analysis is the most common and accurate way of estimating all components of the water relationships in leaves (water potential isotherms) as summarized in the Höfler diagram. PV curve analysis yields values of osmotic pressure, turgor pressure, and elastic modulus of cell walls as a function of relative water content. It allows the computation of symplasmic/apoplastic water content partitioning. For about 20 years, cavitation in xylem has been postulated as a possible source of error when estimating the above parameters, but, to the best of the authors' knowledge, no one has ever previously quantified its influence. Results in this paper provide independent estimates of osmotic pressure by PV curve analysis and by thermocouple psychrometer measurement. An anatomical evaluation was also used for the first time to compare apoplastic water fraction estimates from PV analysis with anatomical values. Conclusions include: (i) PV curve values of osmotic pressure are underestimated prior to correcting osmotic pressure for water loss by cavitation in Metasequoia glyptostroboides; (ii) psychrometer estimates of osmotic pressure obtained in tissues killed by freezing or heating agreed with PV values before correction for apoplastic water dilution; (iii) after correction for dilution effects, a solute concentration enhancement (0.27MPa or 0.11 osmolal) was revealed. The possible sources of solute enhancement were starch hydrolysis and release of ions from the Donnan free space of needle cell walls.
    MeSH term(s) Cupressaceae/physiology ; Osmotic Pressure/physiology ; Plant Leaves/physiology ; Water/metabolism ; Xylem/physiology
    Chemical Substances Water (059QF0KO0R)
    Language English
    Publishing date 2016-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 2976-2
    ISSN 1460-2431 ; 0022-0957
    ISSN (online) 1460-2431
    ISSN 0022-0957
    DOI 10.1093/jxb/erw234
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  5. Article ; Online: Expression of LMP and EBNA genes in Epstein-Barr virus-associated lymphomas in Hu-PBL/SCID mice.

    Tang, Yunlian / Lu, Suli / Gan, Xiaoning / Liu, Fang / Zhang, Yang / Luo, Chunyan / Pan, Yuxia / Hong, Li / Gan, Ruliang

    Oncology reports

    2016  Volume 35, Issue 2, Page(s) 905–911

    Abstract: Transplantation of peripheral blood lymphocytes (PBLs) from healthy humans with latent Epstein-Barr virus (EBV) infection into severe combined immunodeficiency (SCID) mice results in development of EBV-associated human B-cell lymphoma. However, the ... ...

    Abstract Transplantation of peripheral blood lymphocytes (PBLs) from healthy humans with latent Epstein-Barr virus (EBV) infection into severe combined immunodeficiency (SCID) mice results in development of EBV-associated human B-cell lymphoma. However, the expression of EBV genes in relation to lymphoma development has not been reported. We investigated latent membrane protein (LMP) and EBV nuclear antigen (EBNA) gene expression in PBLs from EBV-positive blood donors and induced-lymphoma cells from SCID mice to elucidate the functions and effects of the EBV genome in the occurrence and development of lymphoma. PBLs were isolated from 9 healthy blood donors and transplanted into SCID mice. Gene expression levels of LMP-1, LMP-2A, and LMP-2B and EBNA-1, EBNA-2, EBNA-3A, EBNA-3B, EBNA-3C and EBNA-LP were monitored by real-time quantitative-polymerase chain reaction (qRT-PCR) in cells from nine EBV-induced lymphomas and in matched lymphocytes from healthy subjects. LMP-1, EBNA-1 and EBNA-2 protein levels were detected by western blotting. As a result, LMP-1, LMP-2A and LMP-2B mRNA levels were upregulated 256-, 38- and 331-fold, respectively, in the EBV-induced lymphoma cells compared with the controls, while EBNA-1 and EBNA-3A mRNA levels were upregulated 1157- and 1154-fold, respectively. EBNA-2, EBNA-3B, EBNA-3C and EBNA-LP mRNAs were detected in lymphoma cells, but not in lymphocytes from EBV-positive blood donors. LMP-1 and EBNA-2 proteins were not expressed in lymphocytes from EBV-positive blood donors, according to western blotting. Weak EBNA-1 expression was observed in lymphocytes from blood donors with latent EBV infection, while LMP-1, EBNA-1 and EBNA-2 protein levels were significantly upregulated in EBV-induced lymphoma cells, consistent with mRNA expression levels detected by qRT-PCR. In conclusion, LMP-1, LMP-2A, LMP-2B, EBNA-1 and EBNA-3A were upregulated in EBV-induced lymphoma cells, while EBNA-2, EBNA-3B, EBNA-3C and EBNA-LP were absent in lymphocytes from humans with latent EBV infection, but were positively expressed in EBV-induced lymphoma cells.
    MeSH term(s) Animals ; Blotting, Western ; Epstein-Barr Virus Infections/virology ; Epstein-Barr Virus Nuclear Antigens/biosynthesis ; Epstein-Barr Virus Nuclear Antigens/genetics ; Heterografts ; Humans ; Lymphoma, B-Cell/virology ; Mice ; Mice, SCID ; Polymerase Chain Reaction ; RNA, Viral/analysis ; Transplantation Chimera ; Viral Matrix Proteins/biosynthesis ; Viral Matrix Proteins/genetics
    Chemical Substances EBV-associated membrane antigen, Epstein-Barr virus ; Epstein-Barr Virus Nuclear Antigens ; RNA, Viral ; Viral Matrix Proteins
    Language English
    Publishing date 2016-02
    Publishing country Greece
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1222484-4
    ISSN 1791-2431 ; 1021-335X
    ISSN (online) 1791-2431
    ISSN 1021-335X
    DOI 10.3892/or.2015.4401
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    Zs.A 4213: Show issues Location:
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  6. Article ; Online: Characterization and determination of antioxidant components in the leaves of Camellia chrysantha (Hu) Tuyama based on composition-activity relationship approach.

    Wei, Jin-Bin / Li, Xiong / Song, Hui / Liang, Yong-Hong / Pan, Yu-Zheng / Ruan, Jun-Xiang / Qin, Xia / Chen, Yong-Xin / Nong, Cai-Li / Su, Zhi-Heng

    Journal of food and drug analysis

    2014  Volume 23, Issue 1, Page(s) 40–48

    Abstract: Camellia chrysantha (Hu) Tuyama (CCT), an ornamental plant possessing antioxidant activity, has ...

    Abstract Camellia chrysantha (Hu) Tuyama (CCT), an ornamental plant possessing antioxidant activity, has been infused as tea and drank for its health benefits. The antioxidant components in CCT, however, had not been clearly characterized. To quickly identify the antioxidant constituents of CCT, a composition-activity relationship strategy based on ultra high-pressure liquid chromatography coupled with linear ion trap hybrid orbitrap mass spectrometry and orthogonal partial least-squares method has been applied. As a result, 16 variables were found to make significant contributions to the 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Six of them were identified as catechin (1), epicatechin (5), vitexin (8), isovitexin (10), quercetin-7-O-β-D-glucopyranoside (12) and kaempferol (16). The strength of 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was found to be 12 > 1 > 5 > 16 > 8 > 10 by validation test. Meanwhile, a liquid chromatography-electrospray ionization-mass spectrometry method was established for quantitative determination of six marker compounds in CCT samples from different preparations. The validation of the method, including linearity, sensitivity (limitation of detection and limitation of quantification), repeatability, precision, stability, and recoveries, was carried out and demonstrated to meet the requirements of quantitative analysis. This is the first report on the comprehensive characterization and determination of chemical constituents in CCT by ultra high-pressure liquid chromatography coupled with linear ion trap hybrid orbitrap mass spectrometry. The results indicate that the composition-activity relationship approach may be a useful method for the discovery of active constituents in natural plants and the quality control of medicinal herbs.
    Language English
    Publishing date 2014-05-09
    Publishing country China (Republic : 1949- )
    Document type Journal Article
    ISSN 2224-6614
    ISSN (online) 2224-6614
    DOI 10.1016/j.jfda.2014.02.003
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  7. Book ; Audio / Video: Hu ju zhong shan

    Pan, Xiping

    Tigers in Zhongshan Mountain

    2005  

    Author's details [Regie: Pan Xiping]
    Language Chinese
    Size 1 DVD (Region 1-8), 142 Min
    Publishing place China
    Document type Book ; Audio / Video
    Note Audiosprache: Chinesisch ; Untertitel: Chinesisch (Kurzzeichen)
    Database Former special subject collection: coastal and deep sea fishing

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  8. Article: Characterization and determination of antioxidant components in the leaves of Camellia chrysantha (Hu) Tuyama based on composition-activity relationship approach

    Wei, Jin-Bin / Li, Xiong / Song, Hui / Liang, Yong-Hong / Pan, Yu-Zheng / Ruan, Jun-Xiang / Qin, Xia / Chen, Yong-Xin / Nong, Cai-Li / Su, Zhi-Heng

    Journal of food and drug analysis

    2015  Volume 23, Issue 1, Page(s) 40

    Language English ; Chinese
    Document type Article
    ZDB-ID 1292535-4
    ISSN 1021-9498
    Database Current Contents Nutrition, Environment, Agriculture

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    Z 6601: Show issues

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  9. Article: Characterization and determination of antioxidant components in the leaves of Camellia chrysantha (Hu) Tuyama based on composition–activity relationship approach

    Wei, Jin-Bin / Cai-Li Nong / Hui Song / Jun-Xiang Ruan / Xia Qin / Xiong Li / Yong-Hong Liang / Yong-Xin Chen / Yu-Zheng Pan / Zhi-Heng Su

    Journal of Food and Drug Analysis. 2015 Mar., v. 23

    2015  

    Abstract: Camellia chrysantha (Hu) Tuyama (CCT), an ornamental plant possessing antioxidant activity, has ...

    Abstract Camellia chrysantha (Hu) Tuyama (CCT), an ornamental plant possessing antioxidant activity, has been infused as tea and drank for its health benefits. The antioxidant components in CCT, however, had not been clearly characterized. To quickly identify the antioxidant constituents of CCT, a composition–activity relationship strategy based on ultra high-pressure liquid chromatography coupled with linear ion trap hybrid orbitrap mass spectrometry and orthogonal partial least-squares method has been applied. As a result, 16 variables were found to make significant contributions to the 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Six of them were identified as catechin (1), epicatechin (5), vitexin (8), isovitexin (10), quercetin-7-O-β-D-glucopyranoside (12) and kaempferol (16). The strength of 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was found to be 12 > 1 > 5 > 16 > 8 > 10 by validation test. Meanwhile, a liquid chromatography-electrospray ionization-mass spectrometry method was established for quantitative determination of six marker compounds in CCT samples from different preparations. The validation of the method, including linearity, sensitivity (limitation of detection and limitation of quantification), repeatability, precision, stability, and recoveries, was carried out and demonstrated to meet the requirements of quantitative analysis. This is the first report on the comprehensive characterization and determination of chemical constituents in CCT by ultra high-pressure liquid chromatography coupled with linear ion trap hybrid orbitrap mass spectrometry. The results indicate that the composition–activity relationship approach may be a useful method for the discovery of active constituents in natural plants and the quality control of medicinal herbs.
    Keywords 2,2-diphenyl-1-picrylhydrazyl ; antioxidant activity ; antioxidants ; Camellia ; catechin ; chemical composition ; drugs ; epicatechin ; high performance liquid chromatography ; kaempferol ; least squares ; leaves ; mass spectrometry ; medicinal plants ; ornamental plants ; quality control ; quantitative analysis ; tea
    Language English
    Dates of publication 2015-03
    Size p. 40-48.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1292535-4
    ISSN 1021-9498
    ISSN 1021-9498
    DOI 10.1016/j.jfda.2014.02.003
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: Polymorphisms of the prion protein gene and their effects on litter size and risk evaluation for scrapie in Chinese Hu sheep.

    Guan, Feng / Pan, Lei / Li, Jie / Tang, Hong / Zhu, Cheng / Shi, Guoqing

    Virus genes

    2011  Volume 43, Issue 1, Page(s) 147–152

    Abstract: ... 171 and other six loci (at codons 101, 112, 127, 137, 138, and 152) were detected in 180 Chinese Hu ... of PRNP in Chinese Hu sheep. It could be concluded that Hu sheep has a low susceptibility to natural ...

    Abstract It is well known that scrapie is a fatal, neurodegenerative disease in sheep and goat, which belongs to the group of transmissible spongiform encephalopathies (TSEs) or prion diseases. It has been confirmed that the polymorphisms of prion protein gene (PRNP) at codons 136, 154, and 171 have strong relationship with scrapie in sheep. In the present study, nine polymorphisms of PRNP at codons 136, 154, and 171 and other six loci (at codons 101, 112, 127, 137, 138, and 152) were detected in 180 Chinese Hu sheep. All the alleles at codons 136, 154, and 171 have been identified and resulted in three new genotypes. The frequencies of predominant alleles were 85% (A136), 99.40% (R154), and 37.78% (Q171), respectively. The predominant haplotype ARQ has a relatively high frequency of 57.77%. The frequencies of dominant genotypes of ARR/ARQ and ARQ/ARQ were 30 and 26.67%, respectively. Three new found genotypes named ARQ/TRK, ARQ/TRR, and TRR/TRQ had the same lower frequencies (0.56%). The relationship of PRNP genotype with scrapie risk and litter size showed that the predominant genotypes are corresponded to the risk score of R(1) (1.67%), R(2) (32.22%), and R(3) (42.22%). Just at the first parity, the individuals with ARH/ARH genotype had significantly larger litter size than the mean value and those with ARQ/ARQ and ARR/ARQ genotypes. In short, this study provided preliminary information about alleles and genotypes of PRNP in Chinese Hu sheep. It could be concluded that Hu sheep has a low susceptibility to natural scrapie, and the predominant PRNP genotype at least has no significant effect on litter size.
    MeSH term(s) Animals ; China/epidemiology ; Gene Frequency ; Haplotypes ; Litter Size/genetics ; Polymorphism, Genetic ; Prions/genetics ; Scrapie/epidemiology ; Scrapie/genetics ; Sheep
    Chemical Substances Prions
    Language English
    Publishing date 2011-05-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639496-6
    ISSN 1572-994X ; 0920-8569
    ISSN (online) 1572-994X
    ISSN 0920-8569
    DOI 10.1007/s11262-011-0609-5
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