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Article ; Online: Key amino acid residues in homoserine-acetyltransferase from M. tuberculosis give insight into the evolution of MetX family of enzymes - HAT, SAT and HST.

Maurya, Bhavna / Colaço, Melwin / Wouters, Johan / Pochet, Lionel / Misquith, Sandra

2021 Volume 189, Page(s) 13–25

Abstract: ... to Gly and Pro respectively in homoserine-acetyltransferase from M. tuberculosis resulted ... in a serine-acetyltransferase like enzyme as it acetylated both l-homoserine and l-serine. Homoserine-acetyltransferase from M ...

Abstract	Multiple sequence alignment of homoserine-acetyltransferases, serine-acetyltransferases and homoserine-succinyltransferases show they all belong to MetX family, having evolved from a common ancestor by conserving the catalytic site and substrate binding residues. The discrimination in the substrate selection arises due to the presence of substrate-specific residues lining the substrate-binding pocket. Mutation of Ala59 and Gly62 to Gly and Pro respectively in homoserine-acetyltransferase from M. tuberculosis resulted in a serine-acetyltransferase like enzyme as it acetylated both l-homoserine and l-serine. Homoserine-acetyltransferase from M. tuberculosis when mutated at positon 322 where Leu was converted to Arg, resulted in succinylation over acetylation of l-homoserine. Our studies establish the importance of the substrate binding residues in determining the type of activity possessed by MetX family, despite all of them having the same catalytic triad Ser-Asp-His. Hence key residues at the substrate binding pocket dictate whether the given enzyme shows predominant transferase or hydrolase activity.
MeSH term(s)	Acetyltransferases/chemistry ; Acetyltransferases/genetics ; Arginine/chemistry ; Arginine/genetics ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Homoserine O-Succinyltransferase/chemistry ; Homoserine O-Succinyltransferase/genetics ; Leucine/chemistry ; Leucine/genetics ; Mycobacterium tuberculosis/enzymology ; Mycobacterium tuberculosis/genetics ; Serine O-Acetyltransferase/chemistry ; Serine O-Acetyltransferase/genetics
Chemical Substances	Bacterial Proteins ; Arginine (94ZLA3W45F) ; Acetyltransferases (EC 2.3.1.-) ; Serine O-Acetyltransferase (EC 2.3.1.30) ; homoserine O-acetyltransferase (EC 2.3.1.31) ; Homoserine O-Succinyltransferase (EC 2.3.1.46) ; Leucine (GMW67QNF9C)
Language	English
Publishing date	2021-06-04
Publishing country	France
Document type	Journal Article
ZDB-ID	120345-9
ISSN	1638-6183 ; 0300-9084
ISSN (online)	1638-6183
ISSN	0300-9084
DOI	10.1016/j.biochi.2021.05.016
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Article: Key amino acid residues in homoserine-acetyltransferase from M. tuberculosis give insight into the evolution of MetX family of enzymes – HAT, SAT and HST

Maurya, Bhavna / Colaço, Melwin / Wouters, Johan / Pochet, Lionel / Misquith, Sandra

Biochimie. 2021 Oct., v. 189

2021

Abstract	Multiple sequence alignment of homoserine-acetyltransferases, serine-acetyltransferases and homoserine-succinyltransferases show they all belong to MetX family, having evolved from a common ancestor by conserving the catalytic site and substrate binding residues. The discrimination in the substrate selection arises due to the presence of substrate-specific residues lining the substrate-binding pocket. Mutation of Ala59 and Gly62 to Gly and Pro respectively in homoserine-acetyltransferase from M. tuberculosis resulted in a serine-acetyltransferase like enzyme as it acetylated both l-homoserine and l-serine. Homoserine-acetyltransferase from M. tuberculosis when mutated at positon 322 where Leu was converted to Arg, resulted in succinylation over acetylation of l-homoserine. Our studies establish the importance of the substrate binding residues in determining the type of activity possessed by MetX family, despite all of them having the same catalytic triad Ser-Asp-His. Hence key residues at the substrate binding pocket dictate whether the given enzyme shows predominant transferase or hydrolase activity.
Keywords	Mycobacterium tuberculosis ; acetylation ; active sites ; ancestry ; evolution ; hydrolases ; mutation ; sequence alignment ; serine
Language	English
Dates of publication	2021-10
Size	p. 13-25.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	120345-9
ISSN	0300-9084
ISSN	0300-9084
DOI	10.1016/j.biochi.2021.05.016
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Development and validation of the Leuven intelligibility sentence test with male speaker (LIST-m).

Jansen, Sofie / Koning, Raphael / Wouters, Jan / van Wieringen, Astrid

International journal of audiology

2014 Volume 53, Issue 1, Page(s) 55–59

Abstract: ... between the LIST with male and female voice.: Conclusions: The LIST-m is a reliable and valid ... Objective: In addition to the LIST with a female speaker ( van Wieringen & Wouters, 2008 ), a new ...

Abstract	Objective: In addition to the LIST with a female speaker ( van Wieringen & Wouters, 2008 ), a new speech perception test with a male voice was developed and validated, for evaluating the intelligibility performance of cochlear implant (CI) users or severely hearing-impaired persons. Design: Three experimental steps were carried out: (1) a perceptual optimization of the recorded materials, (2) an evaluation in normal-hearing (NH) listeners, and (3) a validation in CI-users. Measurements were performed both in quiet and in noise. Study sample: Forty-four NH subjects and six CI-users participated. Results: After selecting the sentences with a similar intelligibility, the reference psychometric curve for NH listeners was determined, showing steep slopes for measurements in quiet (12.3%/dB) and in noise (18.7%/dB), similar to the LIST with female voice. The 38 lists of 10 sentences yielded equal scores, and the within-subject test-retest reliability was high (1.7 dB in quiet, 1.1 dB in noise). For the CI-users, parallel psychometric curves were found between the LIST with male and female voice. Conclusions: The LIST-m is a reliable and valid speech intelligibility test that can be used for CI-users, both in quiet and in noise.
MeSH term(s)	Acoustic Stimulation/methods ; Adolescent ; Adult ; Aged ; Audiometry, Speech/methods ; Auditory Threshold ; Case-Control Studies ; Cochlear Implantation/instrumentation ; Cochlear Implants ; Correction of Hearing Impairment/instrumentation ; Female ; Humans ; Male ; Middle Aged ; Noise/adverse effects ; Perceptual Masking ; Persons With Hearing Impairments/psychology ; Persons With Hearing Impairments/rehabilitation ; Predictive Value of Tests ; Psychometrics ; Reproducibility of Results ; Sex Factors ; Speech Intelligibility ; Speech Perception ; Young Adult
Language	English
Publishing date	2014-01
Publishing country	England
Document type	Comparative Study ; Journal Article ; Validation Studies
ZDB-ID	2073098-6
ISSN	1708-8186 ; 1499-2027
ISSN (online)	1708-8186
ISSN	1499-2027
DOI	10.3109/14992027.2013.839886
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Online: Chapter 2 Healthcare and technology

Wouters, Eveline J.M

The multi-level perspective: theories, models, and frameworks

2022

Keywords	Sociology ; elder care, older people, care, digital agency, digitalization, public health, social services, ageism
Language	English
Size	1 electronic resource (19 pages)
Publisher	Taylor and Francis
Document type	Book ; Online
Note	English
HBZ-ID	HT030383049
ISBN	9780367725594 ; 0367725592
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article: Energy balance at high altitude of 6,542 m.

Westerterp, K R / Kayser, B / Wouters, L / Le Trong, J L / Richalet, J P

Journal of applied physiology (Bethesda, Md. : 1985)

1994 Volume 77, Issue 2, Page(s) 862–866

Abstract: ... in high-altitude climbers. Up to approximately 5,000 m, energy balance may be attained and intestinal ... energy digestibility remains normal. To see whether 1) energy balance may also be attained at 6,542 m and ... men; 27-44 yr) were assessed during a 21-day sojourn on the summit of Mt. Sajama, Bolivia (6,542 m ...

Abstract	Weight loss due to malnutrition and possibly intestinal malabsorption is a well-known phenomenon in high-altitude climbers. Up to approximately 5,000 m, energy balance may be attained and intestinal energy digestibility remains normal. To see whether 1) energy balance may also be attained at 6,542 m and, if not, 2) whether decreased energy digestibility would play a significant role in the energy deficit, energy intake (EI), energy expenditure, body composition, and energy digestibility of 10 subjects (4 women, 6 men; 27-44 yr) were assessed during a 21-day sojourn on the summit of Mt. Sajama, Bolivia (6,542 m). EI was measured during two 3-day intervals: EI1 (days 7-9) and EI2 (days 17-19). Total fecal energy loss during EI1 was calculated from fecal energy measured by bomb calorimetry. Average daily metabolic rate (ADMR) at altitude was measured in six subjects (2 women, 4 men) using doubly labeled water over a 10-day interval (days 9-19). Basal metabolic rate was measured before and after the expedition by respiratory gas analysis. Body composition was estimated from skinfolds and body mass before and during the altitude sojourn. Subjects were in negative energy balance throughout the observation period (EI1-ADMR = -2.9 +/- 1.8 MJ/day and EI2-ADMR = -2.3 +/- 1.8 MJ/day based on a gross energy digestibility of 95%). The activity level, expressed as ADMR to basal metabolic rate, was 1.56-2.39. The loss of fat mass (3.7 +/- 1.5 kg) represented 74 +/- 15% of the loss of body mass.(ABSTRACT TRUNCATED AT 250 WORDS)
MeSH term(s)	Acclimatization/physiology ; Adult ; Altitude ; Body Composition/physiology ; Body Water/physiology ; Digestion/physiology ; Eating/physiology ; Energy Metabolism/physiology ; Feces/chemistry ; Female ; Humans ; Male ; Nitrogen/metabolism ; Sleep/physiology
Chemical Substances	Nitrogen (N762921K75)
Language	English
Publishing date	1994-08
Publishing country	United States
Document type	Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	219139-8
ISSN	1522-1601 ; 8750-7587 ; 0021-8987 ; 0161-7567
ISSN (online)	1522-1601
ISSN	8750-7587 ; 0021-8987 ; 0161-7567
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Online: Digital Transformations in Care for Older People

Hirvonen, Helena / Tammelin, Mia / Hänninen, Riitta / Wouters, Eveline J.M.

Critical Perspectives

2022

Keywords	Sociology ; elder care, older people, care, digital agency, digitalization, public health, social services, ageism
Language	0\|e
Size	1 Online-Ressource
Publisher	Taylor and Francis
Document type	Book ; Online
Note	English ; Open Access
HBZ-ID	HT021616789
ISBN	9781003155317 ; 1003155316
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Book ; Online: Chapter 5 Ageism in applying digital technology in healthcare

Mannheim, Ittay / van Zaalen, Yvonne / Wouters, Eveline J.M

Implications for adoption and actual use

2022

Keywords	Sociology ; elder care, older people, care, digital agency, digitalization, public health, social services, ageism
Language	English
Size	1 electronic resource (20 pages)
Publisher	Taylor and Francis
Document type	Book ; Online
Note	English
HBZ-ID	HT030382798
ISBN	9780367725594 ; 0367725592
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Book ; Online: Ice loss in the Canadian Arctic Archipelago, supplementary data to: Gardner, A S; Moholdt, G; Wouters, B; Wolken, G J; Burgess, D O; Sharp, M J; Cogley, J G; Braun, C; Labine, C (2011): Sharply increased mass loss from glaciers and ice caps in the Canadian Arctic Archipelago. Nature, 473(7347), 357-360

Gardner, A S / Braun, C / Burgess, D O / Cogley, J G / Labine, C / Moholdt, G / Observatory, NASA Earth / Sharp, M J / Wolken, G J / Wouters, B

2011

Abstract: Though much attention has been focused in recent years on the melting of ice from Greenland and Antarctica, nearly half of the ice volume currently being lost to the ocean is actually coming from other mountain glaciers and ice caps. Ice loss from a ... ...

Abstract	Though much attention has been focused in recent years on the melting of ice from Greenland and Antarctica, nearly half of the ice volume currently being lost to the ocean is actually coming from other mountain glaciers and ice caps. Ice loss from a group of islands in northern Canada accounts for much of that volume. In a study published in April 2011 in the journal Nature, a team of researchers led by Alex Gardner of the University of Michigan found that land ice in both the northern and southern Canadian Arctic Archipelago has declined sharply. The maps above show ice loss from surface melting for the northern portion of the archipelago from 2004-2006 (left) and 2007-2009 (right). Blue indicates ice gain, and red indicates ice loss. In the six years studied, the Canadian Arctic Archipelago lost an average of approximately 61 gigatons of ice per year. (A gigaton is a billion tons of ice.) The research team also found the rate of ice loss was accelerating. From 2004 to 2006, the average mass loss was roughly 31 gigatons per year; from 2007 to 2009, the loss increased to 92 gigatons per year. Gardner and colleagues used three independent methods to assess ice mass, all of which showed the same trends. The team used a model to estimate the surface mass balance of ice and the amount of ice discharged. They also compiled and analyzed measurements from NASA's Ice, Cloud and Land Elevation Satellite (ICESat) to assess changes in the surface height of ice. Finally, they gathered observations from NASA's Gravity Recovery and Climate Experiment (GRACE) to determine changes in the gravity field in the region, an indicator of the amount of ice gained or lost. The Canadian Arctic Archipelago generally receives little precipitation, and the amount of snowfall changes little from year to year. But the rate of snow and ice melting varies considerably, so changes in ice mass come largely from changes in summertime melt. During the 2004 to 2009 study period, the Canadian Arctic Archipelago experienced four of its five warmest years since 1960, likely fueling the melting. Gardner notes that from 2001 to 2004, the sum of melting from all mountain glaciers and ice caps around the world (but not the Greenland and Antarctic ice sheets) contributed an estimated 1 millimeter per year to global sea level rise. Recent estimates suggest the Greenland and Antarctic ice sheets add another 1.3 millimeters per year to sea level. "This means 1 percent of the land ice volume-mountain glaciers and ice caps-account for about half of all ice loss to the world's oceans," Gardner said. "Most of the ice loss is coming from the Canadian Arctic Archipelago, Alaska, Patagonia, the Himalayas, and the smaller ice masses surrounding the main Greenland and Antarctic ice sheets."
Language	English
Dates of publication	2011-9999
Size	Online-Ressource
Publisher	PANGAEA - Data Publisher for Earth & Environmental Science
Publishing place	Bremen/Bremerhaven
Document type	Book ; Online
Note	This dataset is supplement to doi:10.1038/nature10089
DOI	10.1594/PANGAEA.761481
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Article: Water balance and acute mountain sickness before and after arrival at high altitude of 4,350 m.

Westerterp, K R / Robach, P / Wouters, L / Richalet, J P

Journal of applied physiology (Bethesda, Md. : 1985)

1996 Volume 80, Issue 6, Page(s) 1968–1972

Abstract: ... interval before and a subsequent 4-day interval after transport to 4,350 m. Total body water and ...

Abstract	The present study is a first attempt to measure water balance and its components at altitude by using labeled water and bromide dilution and relating the results with acute mountain sickness (AMS). Water intake, total water output, and water output in urine and feces were measured over a 4-day interval before and a subsequent 4-day interval after transport to 4,350 m. Total body water and extracellular water were measured at the start and at the end of the two intervals. There was a close relationship between energy intake and water intake, and the relationship was unchanged by the altitude intervention. Subjects developing AMS reduced energy intake and water intake cor respondingly. The increase in total body water (TBW) in subjects developing AMS was accompanied by a reduction in total water loss. They did not show the increased urine output, compensating for the reduced evaporative water loss at altitude. Subjects showed a significant increase in TBW after 4 days at altitude. Subjects with AMS showed the biggest shifts in extracellular water relative to TBW. In conclusion, fluid retention in relation to AMS is independent of a change in water requirements due to altitude exposure. Subjects developing AMS were those showing a fluid shift of at least 1 liter from the intracellular to the extracellular compartment or from the extracellular to the intracellular compartment.
MeSH term(s)	Adult ; Altitude ; Body Water/metabolism ; Drinking/physiology ; Female ; Humans ; Male ; Middle Aged ; Mountaineering ; Time Factors
Language	English
Publishing date	1996-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	219139-8
ISSN	1522-1601 ; 8750-7587 ; 0021-8987 ; 0161-7567
ISSN (online)	1522-1601
ISSN	8750-7587 ; 0021-8987 ; 0161-7567
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Conference proceedings: Special issue 8th NIZO Dairy Conference

Wouters, Jan T. M.

[the NIZO Dairy Conference was held in Papendal, near Arnhem, in the Netherlands from 11 to 13 September 2013 under the title 'Functional Enzymes for Dairy Applications']

(International dairy journal ; 38,2)

2014

Title variant	8th NIZO Dairy Conference
Institution	Nederlands Instituut voor Zuivelonderzoek
Event/congress	Dairy Conference (8, 2013, Papendal)
Author's details	guest ed. Jan T. M. Wouters
Series title	International dairy journal ; 38,2
Collection
Language	English
Size	S. 81 - 218 : Ill., graph. Darst.
Publisher	Elsevier
Publishing place	Amsterdam u.a
Publishing country	United States
Document type	Book ; Conference proceedings
HBZ-ID	HT018369842
Database	Catalogue ZB MED Nutrition, Environment, Agriculture

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Shelf mark	Loan status	Location
Z 5292 (38,2)	available for loan (reading room)	Freihandmagazin (U1)

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