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  1. Article ; Online: GABA

    Hwang, Yeonggwang / Park, Jung Hoon / Kim, Hyoung-Chun / Shin, Eun-Joo

    Life sciences

    2023  Volume 319, Page(s) 121529

    Abstract: Aims: We examined the effect of γ-aminobutyric acid (GABA): Main methods: Male C57BL/6N mice received TMT (2.6 mg/kg, i.p.), and the expression of GABA: Key findings: TMT insult significantly induced the astroglial expression of GABA: ... ...

    Abstract Aims: We examined the effect of γ-aminobutyric acid (GABA)
    Main methods: Male C57BL/6N mice received TMT (2.6 mg/kg, i.p.), and the expression of GABA
    Key findings: TMT insult significantly induced the astroglial expression of GABA
    Significance: Our results suggest that GABA
    MeSH term(s) Animals ; Male ; Mice ; Astrocytes/metabolism ; Baclofen/pharmacology ; Dentate Gyrus ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Glial Cell Line-Derived Neurotrophic Factor/metabolism ; Hippocampus/metabolism ; Mice, Inbred C57BL ; Phenotype ; Receptors, GABA/metabolism ; Receptors, GABA-B
    Chemical Substances Baclofen (H789N3FKE8) ; Extracellular Signal-Regulated MAP Kinases (EC 2.7.11.24) ; Glial Cell Line-Derived Neurotrophic Factor ; Receptors, GABA ; trimethyltin (1631-73-8) ; Receptors, GABA-B
    Language English
    Publishing date 2023-02-23
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 3378-9
    ISSN 1879-0631 ; 0024-3205
    ISSN (online) 1879-0631
    ISSN 0024-3205
    DOI 10.1016/j.lfs.2023.121529
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Synthesis and Characterization of Biopolyol-Based Waterborne Polyurethane Modified through Complexation with Chitosan

    Soon-Mo Choi / Sunhee Lee / Eun-Joo Shin

    Nanomaterials, Vol 12, Iss 1143, p

    2022  Volume 1143

    Abstract: In this study, a series of castor oil-based anionic waterborne polyurethane (CWPU) systems, which it has been suggested may be suitable for use as green elastomers with diverse applications in films and coatings, was prepared by modified with O- ... ...

    Abstract In this study, a series of castor oil-based anionic waterborne polyurethane (CWPU) systems, which it has been suggested may be suitable for use as green elastomers with diverse applications in films and coatings, was prepared by modified with O-carboxymethyl chitosan (CS) as not only a reinforcing filler, but a chain-extender of polyurethane prepolymer to enhance the properties of polyurethanes. Moreover, not only was the system obtained with castor oil-based polyol in the absence of a catalyst, but it was maintained with low viscosity by using acetone instead of toxic methyl ethyl ketone (MEK) during the synthesis process. The sizes, zeta potential, chemical formation, and morphology of the CWPU-CS composites had been investigated by dynamic light scattering (DLS), infrared spectroscopy (IR), and scanning electron microscopy (SEM). Moreover, the results show that the modification allows to enhance storage/loss modulus, tensile properties, thermal stability at high temperature, and biocompatibility of CWPU and CWPU/CS nanocomposites according to various contents of CS.
    Keywords waterborne polyurethane ; chitosan ; reinforcement ; Chemistry ; QD1-999
    Subject code 620
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: Synthesis of Novel Shape Memory Thermoplastic Polyurethanes (SMTPUs) from Bio-Based Materials for Application in 3D/4D Printing Filaments.

    Jung, Yang-Sook / Lee, Sunhee / Park, Jaehyeung / Shin, Eun-Joo

    Materials (Basel, Switzerland)

    2023  Volume 16, Issue 3

    Abstract: Bio-based thermoplastic polyurethanes have attracted increasing attention as advanced shape memory materials. Using the prepolymer method, novel fast-responding shape memory thermoplastic polyurethanes (SMTPUs) were prepared from 100% bio-based polyester ...

    Abstract Bio-based thermoplastic polyurethanes have attracted increasing attention as advanced shape memory materials. Using the prepolymer method, novel fast-responding shape memory thermoplastic polyurethanes (SMTPUs) were prepared from 100% bio-based polyester polyol, poly-propylene succinate derived from corn oil, diphenyl methane diisocyanate, and bio-based 1,3-propanediol as a chain extender. The morphologies of the SMTPUs were investigated by Fourier transform infrared spectroscopy, atomic force microscopy, and X-ray diffraction, which revealed the interdomain spacing between the hard and soft phases, the degree of phase separation, and the intermixing level between the hard and soft phases. The thermal and mechanical properties of the SMTPUs were also investigated, wherein a high hard segment content imparted unique properties that rendered the SMTPUs suitable for shape memory applications at varying temperatures. More specifically, the SMTPUs exhibited a high level of elastic elongation and good mechanical strength. Following compositional optimization, a tensile strength of 24-27 MPa was achieved, in addition to an elongation at break of 358-552% and a hardness of 84-92 Shore A. Moreover, the bio-based SMTPU exhibited a shape recovery of 100%, thereby indicating its potential for use as an advanced temperature-dependent shape memory material with an excellent shape recoverability.
    Language English
    Publishing date 2023-01-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma16031072
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Limaprost and the Risk of Bleeding: A Self-Controlled Case Series Study.

    Lee, Eun-Joo / Jeong, Han Eol / Chang, Yoosoo / Shin, Ju-Young

    Neurospine

    2023  Volume 20, Issue 4, Page(s) 1490–1500

    Abstract: Objective: To investigate the association between the use of limaprost and the risk of bleeding.: Methods: A self-controlled case series analysis was conducted using the National Health Insurance Service-National Sample Cohort database in South Korea. ...

    Abstract Objective: To investigate the association between the use of limaprost and the risk of bleeding.
    Methods: A self-controlled case series analysis was conducted using the National Health Insurance Service-National Sample Cohort database in South Korea. We identified patients aged 18 years or older who had at least one prescription of limaprost and were diagnosed with at least one case of bleeding between 2003 and 2019. The incidence rate ratio (IRR) of bleeding was calculated by dividing the incidence rate in the exposed period to limaprost by that in the unexposed period and adjusted for age using conditional Poisson regression model.
    Results: Among 72,860 patients with limaprost prescriptions and bleeding diagnoses, there were 184,732 events of bleeding. After adjusting for age, the IRR was 1.47 (95% confidence interval [CI], 1.43-1.50), wherein the IRR was the highest during the 0-7 days after limaprost initiation (IRR, 2.11; 95% CI, 2.03-2.18). Risk of bleeding was higher when limaprost was concomitantly used with antithrombotics or other drugs for spinal stenosis treatment, and when higher daily doses of limaprost were administered.
    Conclusion: Our findings suggest that the risk of bleeding increased by 1.5-fold in periods of limaprost exposure compared to unexposed periods, with particularly higher risks observed during the first week after limaprost initiation, with concomitant drugs related to bleeding, and with a higher daily dose. A careful risk-benefit assessment is warranted when initiating limaprost, especially when administered with other medications or in higher daily doses.
    Language English
    Publishing date 2023-12-31
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 3031654-6
    ISSN 2586-6591 ; 2586-6583
    ISSN (online) 2586-6591
    ISSN 2586-6583
    DOI 10.14245/ns.2346640.320
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Synthesis and Characterization of Biopolyol-Based Waterborne Polyurethane Modified through Complexation with Chitosan.

    Choi, Soon-Mo / Lee, Sunhee / Shin, Eun-Joo

    Nanomaterials (Basel, Switzerland)

    2022  Volume 12, Issue 7

    Abstract: In this study, a series of castor oil-based anionic waterborne polyurethane (CWPU) systems, which it has been suggested may be suitable for use as green elastomers with diverse applications in films and coatings, was prepared by modified with O- ... ...

    Abstract In this study, a series of castor oil-based anionic waterborne polyurethane (CWPU) systems, which it has been suggested may be suitable for use as green elastomers with diverse applications in films and coatings, was prepared by modified with O-carboxymethyl chitosan (CS) as not only a reinforcing filler, but a chain-extender of polyurethane prepolymer to enhance the properties of polyurethanes. Moreover, not only was the system obtained with castor oil-based polyol in the absence of a catalyst, but it was maintained with low viscosity by using acetone instead of toxic methyl ethyl ketone (MEK) during the synthesis process. The sizes, zeta potential, chemical formation, and morphology of the CWPU-CS composites had been investigated by dynamic light scattering (DLS), infrared spectroscopy (IR), and scanning electron microscopy (SEM). Moreover, the results show that the modification allows to enhance storage/loss modulus, tensile properties, thermal stability at high temperature, and biocompatibility of CWPU and CWPU/CS nanocomposites according to various contents of CS.
    Language English
    Publishing date 2022-03-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662255-5
    ISSN 2079-4991
    ISSN 2079-4991
    DOI 10.3390/nano12071143
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: BKM120 alters the migration of doublecortin-positive cells in the dentate gyrus of mice.

    Hwang, Yeonggwang / Kim, Hyoung-Chun / Shin, Eun-Joo

    Pharmacological research

    2022  Volume 179, Page(s) 106226

    Abstract: BKM120 is an inhibitor of class I phosphoinositide 3-kinases and its anti-cancer effects have been demonstrated in various solid cancer models. BKM120 is highly brain permeable and has been reported to induce mood disturbances in clinical trials. ... ...

    Abstract BKM120 is an inhibitor of class I phosphoinositide 3-kinases and its anti-cancer effects have been demonstrated in various solid cancer models. BKM120 is highly brain permeable and has been reported to induce mood disturbances in clinical trials. Therefore, we examined whether BKM120 produces anxiety- and depression-like behaviors in mice, as with patients receiving BKM120 in clinical trials. In this study, repeated BKM120 treatment (2.0 or 5.0 mg/kg, i.p., five times at 12-h interval) significantly induced anxiety- and depression-like behaviors in mice. Although abnormal changes in hippocampal neurogenesis have been suggested to, at least in part, associated with the pathogenesis of depression and anxiety, BKM120 did not affect the incorporation of 5-bromo-2'-deoxyuridine or the expression of doublecortin (DCX); however, it significantly enhanced the radial migration of DCX-positive cells in the dentate gyrus. BKM120-induced changes in migration were not accompanied by obvious neuronal damage in the hippocampus. Importantly, BKM120-induced anxiety- and depression-like behaviors were positively correlated with the extent of DCX-positive cell migration. Concomitantly, p-Akt expression was significantly decreased in the dentate gyrus. Moreover, the expression of p-c-Jun N-terminal kinase (JNK), p-DCX, and Ras homolog family member A (RhoA)-GTP decreased significantly, particularly in aberrantly migrated DCX-positive cells. Together, the results suggest that repeated BKM120 treatment enhances the radial migration of DCX-positive cells and induces anxiety- and depression-like behaviors by regulating the activity of Akt, JNK, DCX, and RhoA in the dentate gyrus. It also suggests that the altered migration of adult-born neurons in the dentate gyrus plays a role in mood disturbances.
    MeSH term(s) Aminopyridines ; Animals ; Dentate Gyrus/metabolism ; Doublecortin Domain Proteins ; Doublecortin Protein ; Hippocampus/metabolism ; Humans ; Mice ; Microtubule-Associated Proteins/metabolism ; Morpholines ; Neuropeptides/metabolism ; Proto-Oncogene Proteins c-akt/metabolism
    Chemical Substances Aminopyridines ; Doublecortin Domain Proteins ; Doublecortin Protein ; Microtubule-Associated Proteins ; Morpholines ; NVP-BKM120 ; Neuropeptides ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1)
    Language English
    Publishing date 2022-04-20
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1003347-6
    ISSN 1096-1186 ; 0031-6989 ; 1043-6618
    ISSN (online) 1096-1186
    ISSN 0031-6989 ; 1043-6618
    DOI 10.1016/j.phrs.2022.106226
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Effect of rottlerin on astrocyte phenotype polarization after trimethyltin insult in the dentate gyrus of mice.

    Hwang, Yeonggwang / Kim, Hyoung-Chun / Shin, Eun-Joo

    Journal of neuroinflammation

    2022  Volume 19, Issue 1, Page(s) 142

    Abstract: Background: It has been demonstrated that reactive astrocytes can be polarized into pro-inflammatory A1 phenotype or anti-inflammatory A2 phenotype under neurotoxic and neurodegenerative conditions. Microglia have been suggested to play a critical role ... ...

    Abstract Background: It has been demonstrated that reactive astrocytes can be polarized into pro-inflammatory A1 phenotype or anti-inflammatory A2 phenotype under neurotoxic and neurodegenerative conditions. Microglia have been suggested to play a critical role in astrocyte phenotype polarization by releasing pro- and anti-inflammatory mediators. In this study, we examined whether trimethyltin (TMT) insult can induce astrocyte polarization in the dentate gyrus of mice, and whether protein kinase Cδ (PKCδ) plays a role in TMT-induced astrocyte phenotype polarization.
    Methods: Male C57BL/6 N mice received TMT (2.6 mg/kg, i.p.), and temporal changes in the mRNA expression of A1 and A2 phenotype markers were evaluated in the hippocampus. In addition, temporal and spatial changes in the protein expression of C3, S100A10, Iba-1, and p-PKCδ were examined in the dentate gyrus. Rottlerin (5 mg/kg, i.p. × 5 at 12-h intervals) was administered 3-5 days after TMT treatment, and the expression of A1 and A2 transcripts, p-PKCδ, Iba-1, C3, S100A10, and C1q was evaluated 6 days after TMT treatment.
    Results: TMT treatment significantly increased the mRNA expression of A1 and A2 phenotype markers, and the increased expression of A1 markers remained longer than that of A2 markers. The immunoreactivity of the representative A1 phenotype marker, C3 and A2 phenotype marker, S100A10 peaked 6 days after TMT insult in the dentate gyrus. While C3 was expressed evenly throughout the dentate gyrus, S100A10 was highly expressed in the hilus and inner molecular layer. In addition, TMT insult induced microglial p-PKCδ expression. Treatment with rottlerin, a PKCδ inhibitor, decreased Iba-1 and C3 expression, but did not affect S100A10 expression, suggesting that PKCδ inhibition attenuates microglial activation and A1 astrocyte phenotype polarization. Consistently, rottlerin significantly reduced the expression of C1q and tumor necrosis factor-α (TNFα), which has been suggested to be released by activated microglia and induce A1 astrocyte polarization.
    Conclusion: We demonstrated the temporal and spatial profiles of astrocyte polarization after TMT insult in the dentate gyrus of mice. Taken together, our results suggest that PKCδ plays a role in inducing A1 astrocyte polarization by promoting microglial activation and consequently increasing the expression of pro-inflammatory mediators after TMT insult.
    MeSH term(s) Acetophenones ; Animals ; Astrocytes/metabolism ; Benzopyrans ; Complement C1q/metabolism ; Dentate Gyrus/pathology ; Male ; Mice ; Mice, Inbred C57BL ; Microglia/metabolism ; Phenotype ; RNA, Messenger/metabolism ; Trimethyltin Compounds
    Chemical Substances Acetophenones ; Benzopyrans ; RNA, Messenger ; Trimethyltin Compounds ; trimethyltin (1631-73-8) ; Complement C1q (80295-33-6) ; rottlerin (E29LP3ZMUH)
    Language English
    Publishing date 2022-06-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 2156455-3
    ISSN 1742-2094 ; 1742-2094
    ISSN (online) 1742-2094
    ISSN 1742-2094
    DOI 10.1186/s12974-022-02507-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: One-Shot Synthesis of Thermoplastic Polyurethane Based on Bio-Polyol (Polytrimethylene Ether Glycol) and Characterization of Micro-Phase Separation.

    Jung, Yang-Sook / Lee, Sunhee / Park, Jaehyeung / Shin, Eun-Joo

    Polymers

    2022  Volume 14, Issue 20

    Abstract: In this study, a series of bio-based thermoplastic polyurethane (TPU) was synthesized via the solvent-free one-shot method using 100% bio-based polyether polyol, prepared from fermented corn, and 1,4-butanediol (BDO) as a chain extender. The average ... ...

    Abstract In this study, a series of bio-based thermoplastic polyurethane (TPU) was synthesized via the solvent-free one-shot method using 100% bio-based polyether polyol, prepared from fermented corn, and 1,4-butanediol (BDO) as a chain extender. The average molecular weight, degree of phase separation, thermal and mechanical properties of the TPU-based aromatic (4,4-methylene diphenyl diisocyanate: MDI), and aliphatic (bis(4-isocyanatocyclohexyl) methane: H
    Language English
    Publishing date 2022-10-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527146-5
    ISSN 2073-4360 ; 2073-4360
    ISSN (online) 2073-4360
    ISSN 2073-4360
    DOI 10.3390/polym14204269
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Ramelteon attenuates hippocampal neuronal loss and memory impairment following kainate-induced seizures.

    Park, Jung Hoon / Hwang, Yeonggwang / Nguyen, Yen Nhi Doan / Kim, Hyoung-Chun / Shin, Eun-Joo

    Journal of pineal research

    2023  Volume 76, Issue 1, Page(s) e12921

    Abstract: Evidence suggests that the neuroprotective effects of melatonin involve both receptor-dependent and -independent actions. However, little is known about the effects of melatonin receptor activation on the kainate (KA) neurotoxicity. This study examined ... ...

    Abstract Evidence suggests that the neuroprotective effects of melatonin involve both receptor-dependent and -independent actions. However, little is known about the effects of melatonin receptor activation on the kainate (KA) neurotoxicity. This study examined the effects of repeated post-KA treatment with ramelteon, a selective agonist of melatonin receptors, on neuronal loss, cognitive impairment, and depression-like behaviors following KA-induced seizures. The expression of melatonin receptors decreased in neurons, whereas it was induced in astrocytes 3 and 7 days after seizures elicited by KA (0.12 μg/μL) in the hippocampus of mice. Ramelteon (3 or 10 mg/kg, i.p.) and melatonin (10 mg/kg, i.p.) mitigated KA-induced oxidative stress and impairment of glutathione homeostasis and promoted the nuclear translocation and DNA binding activity of Nrf2 in the hippocampus after KA treatment. Ramelteon and melatonin also attenuated microglial activation but did not significantly affect astroglial activation induced by KA, despite the astroglial induction of melatonin receptors after KA treatment. However, ramelteon attenuated KA-induced proinflammatory phenotypic changes in astrocytes. Considering the reciprocal regulation of astroglial and microglial activation, these results suggest ramelteon inhibits microglial activation by regulating astrocyte phenotypic changes. These effects were accompanied by the attenuation of the nuclear translocation and DNA binding activity of nuclear factor κB (NFκB) induced by KA. Consequently, ramelteon attenuated the KA-induced hippocampal neuronal loss, memory impairment, and depression-like behaviors; the effects were comparable to those of melatonin. These results suggest that ramelteon-mediated activation of melatonin receptors provides neuroprotection against KA-induced neurotoxicity in the mouse hippocampus by activating Nrf2 signaling to attenuate oxidative stress and restore glutathione homeostasis and by inhibiting NFκB signaling to attenuate neuroinflammatory changes.
    MeSH term(s) Mice ; Animals ; Melatonin/pharmacology ; Melatonin/metabolism ; Receptors, Melatonin/metabolism ; Kainic Acid/toxicity ; Kainic Acid/metabolism ; NF-E2-Related Factor 2/metabolism ; Hippocampus ; Seizures/chemically induced ; Seizures/drug therapy ; Seizures/metabolism ; Glutathione/metabolism ; DNA ; Indenes
    Chemical Substances Melatonin (JL5DK93RCL) ; Receptors, Melatonin ; Kainic Acid (SIV03811UC) ; ramelteon (901AS54I69) ; NF-E2-Related Factor 2 ; Glutathione (GAN16C9B8O) ; DNA (9007-49-2) ; Indenes
    Language English
    Publishing date 2023-10-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 632697-3
    ISSN 1600-079X ; 0742-3098
    ISSN (online) 1600-079X
    ISSN 0742-3098
    DOI 10.1111/jpi.12921
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Development of a freshness indicator for monitoring the quality of beef during storage.

    Lee, Eun-Joo / Shin, Han-Seung

    Food science and biotechnology

    2019  Volume 28, Issue 6, Page(s) 1899–1906

    Abstract: Quality of beef and detect changes in freshness during storage was evaluated using freshness indicator. The freshness indicator can indicate spoilage or freshness of the packaged product through color changes directly detected by the naked eye. We also ... ...

    Abstract Quality of beef and detect changes in freshness during storage was evaluated using freshness indicator. The freshness indicator can indicate spoilage or freshness of the packaged product through color changes directly detected by the naked eye. We also monitored changes in pH, 2-thiobarbituric acid reactive substances, volatile basic nitrogen, and total bacterial count. A change in the color of the indicator reflects the pH of the headspace of the beef packaging. Beef was stored at 20 °C for 24 h, after which the color of the freshness indicator changed. Results of color change from freshness indicator are similar to quality parameters including chemical, microbiological, and sensory evaluation that are commonly used to characterize the freshness of beef. The results show that this freshness indicator is useful for determining the shelf life of beef products by monitoring physicochemical changes and microbial safety.
    Language English
    Publishing date 2019-06-07
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 2000008-X
    ISSN 2092-6456 ; 1226-7708
    ISSN (online) 2092-6456
    ISSN 1226-7708
    DOI 10.1007/s10068-019-00633-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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