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  1. Article ; Online: Inflammatory myopathies and beyond: The dual role of neutrophils in muscle damage and regeneration.

    Torres-Ruiz, Jiram / Alcalá-Carmona, Beatriz / Alejandre-Aguilar, Ricardo / Gómez-Martín, Diana

    Frontiers in immunology

    2023  Volume 14, Page(s) 1113214

    Abstract: Skeletal muscle is one of the most abundant tissues of the human body and is responsible for the generation of movement. Muscle injuries can lead to severe disability. Skeletal muscle is characterized by an important regeneration capacity, which is ... ...

    Abstract Skeletal muscle is one of the most abundant tissues of the human body and is responsible for the generation of movement. Muscle injuries can lead to severe disability. Skeletal muscle is characterized by an important regeneration capacity, which is possible due to the interaction between the myoblasts and immune cells. Neutrophils are fundamental as inducers of muscle damage and as promoters of the initial inflammatory response which eventually allows the muscle repair. The main functions of the neutrophils are phagocytosis, respiratory burst, degranulation, and the production of neutrophil extracellular traps (NETs). An overactivation of neutrophils after muscle injuries may lead to an expansion of the initial damage and can hamper the successful muscle repair. The importance of neutrophils as inducers of muscle damage extends beyond acute muscle injury and recently, neutrophils have become more relevant as part of the immunopathogenesis of chronic muscle diseases like idiopathic inflammatory myopathies (IIM). This heterogeneous group of systemic autoimmune diseases is characterized by the presence of muscle inflammation with a variable amount of extramuscular features. In IIM, neutrophils have been found to have a role as biomarkers of disease activity, and their expansion in peripheral blood is related to certain clinical features like interstitial lung disease (ILD) and cancer. On the other hand, low density granulocytes (LDG) are a distinctive subtype of neutrophils characterized by an enhanced production of NETs. These cells along with the NETs have also been related to disease activity and certain clinical features like ILD, vasculopathy, calcinosis, dermatosis, and cutaneous ulcers. The role of NETs in the immunopathogenesis of IIM is supported by an enhanced production and deficient degradation of NETs that have been observed in patients with dermatomyositis and anti-synthetase syndrome. Finally, new interest has arisen in the study of other phenotypes of LDG with a phenotype corresponding to myeloid-derived suppressor cells, which were also found to be expanded in patients with IIM and were related to disease activity. In this review, we discuss the role of neutrophils as both orchestrators of muscle repair and inducers of muscle damage, focusing on the immunopathogenesis of IIM.
    MeSH term(s) Humans ; Neutrophils ; Dermatomyositis ; Myositis ; Muscle, Skeletal/pathology ; Lung Diseases, Interstitial ; Muscular Diseases ; Regeneration
    Language English
    Publishing date 2023-02-27
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2023.1113214
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Novel B cell subsets as potential biomarkers in Idiopathic Inflammatory Myopathies: insights into disease pathogenesis and disease activity.

    Reyes-Huerta, Raúl F / Mandujano-López, Vladimir / Velásquez-Ortiz, Guadalupe / Alcalá-Carmona, Beatriz / Ostos-Prado, María J / Reyna-Juárez, Yatzil / Meza-Sánchez, David E / Juárez-Vega, Guillermo / Mejía-Domínguez, Nancy R / Torres-Ruiz, Jiram / Gómez-Martin, Diana / Maravillas-Montero, José L

    Journal of leukocyte biology

    2024  

    Abstract: Idiopathic inflammatory myopathies are a heterogeneous group of rare autoimmune disorders characterized by progressive muscle weakness and the histopathologic findings of inflammatory infiltrates in muscle tissue. Although their pathogenesis remains ... ...

    Abstract Idiopathic inflammatory myopathies are a heterogeneous group of rare autoimmune disorders characterized by progressive muscle weakness and the histopathologic findings of inflammatory infiltrates in muscle tissue. Although their pathogenesis remains indefinite, the association of autoantibodies with clinical manifestations and the evidence of high effectiveness of depleting therapies suggest that B cells could be implicated. Therefore, we explored the landscape of peripheral B cells in this disease by multiparametric flow cytometry, finding significant numerical decreases in memory and double negative subsets, as well as an expansion of the naïve compartment relative to healthy controls, that contribute to defining disease-associated B cell subset signatures and correlating with different clinical features of patients. Additionally, we determined the potential value of these subsets as diagnostic biomarkers, thus positioning B cells as neglected key elements possibly participating in idiopathic inflammatory myopathies onset or development.
    Language English
    Publishing date 2024-03-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 605722-6
    ISSN 1938-3673 ; 0741-5400
    ISSN (online) 1938-3673
    ISSN 0741-5400
    DOI 10.1093/jleuko/qiae083
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Systemic inflammation and cortical neurochemistry in never-medicated first episode-psychosis individuals.

    León-Ortiz, Pablo / Rivera-Chávez, Luis F / Torres-Ruíz, Jiram / Reyes-Madrigal, Francisco / Carrillo-Vázquez, Daniel / Moncada-Habib, Tomás / Cassiano-Quezada, Fabiola / Cadenhead, Kristin S / Gómez-Martín, Diana / de la Fuente-Sandoval, Camilo

    Brain, behavior, and immunity

    2023  Volume 111, Page(s) 270–276

    Abstract: Studies of cellular and cytokine profiles have contributed to the inflammation hypothesis of schizophrenia; however, precise markers of inflammatory dysfunction remain elusive. A number of proton magnetic resonance spectroscopy (1H-MRS) studies in ... ...

    Abstract Studies of cellular and cytokine profiles have contributed to the inflammation hypothesis of schizophrenia; however, precise markers of inflammatory dysfunction remain elusive. A number of proton magnetic resonance spectroscopy (1H-MRS) studies in patients with first-episode psychosis (FEP) have shown higher brain levels of metabolites such as glutamate, myo-inositol (mI) and choline-containing compounds (tCho), suggesting neuroinflammation. Here, we present peripheral inflammatory profiles in antipsychotic-naive FEP patients and age-and-sex matched healthy controls, as well as cortical glutamate, mI and tCho levels using 1H-MRS. Inflammatory profiles were analyzed using cytokine production by peripheral blood mononuclear cells, that were either spontaneous or stimulated, in 48 FEP patients and 23 controls. 1H-MRS of the medial prefrontal cortex was obtained in 29 FEP patients and 18 controls. Finally, 16 FEP patients were rescanned after 4 weeks of treatment (open-label) with Risperidone. FEP patients showed a higher proportion of proinflammatory Th1/Th17 subset, and an increased spontaneous production of Interleukin (IL)-6, IL-2 and IL-4 compared with the control group. Results obtained from 1H-MRS showed no significant difference in either glutamate, mI or tCho between FEP and control groups. At baseline, CD8% showed a negative correlation with glutamate in FEP patients; after 4 weeks of risperidone treatment, the FEP group exhibited a decrease in glutamate levels which positively correlated with CD4 + T cells. Nevertheless, these correlations did not survive correction for multiple comparisons. FEP patients show evidence of immune dysregulation, affecting both the innate and adaptive immune response, with a predominantly Th2 signature. These findings, along with the changes produced by antipsychotic treatment, could be associated with both systemic and central inflammatory processes in schizophrenia.
    MeSH term(s) Humans ; Risperidone/therapeutic use ; Antipsychotic Agents/therapeutic use ; Leukocytes, Mononuclear/metabolism ; Neurochemistry ; Psychotic Disorders ; Glutamic Acid/metabolism ; Interleukin-6 ; Inflammation/complications
    Chemical Substances Risperidone (L6UH7ZF8HC) ; Antipsychotic Agents ; Glutamic Acid (3KX376GY7L) ; Interleukin-6
    Language English
    Publishing date 2023-05-05
    Publishing country Netherlands
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 639219-2
    ISSN 1090-2139 ; 0889-1591
    ISSN (online) 1090-2139
    ISSN 0889-1591
    DOI 10.1016/j.bbi.2023.05.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Patient global assessment and inflammatory markers in patients with idiopathic inflammatory myopathies - A longitudinal study.

    Lodin, Karin / Espinosa-Ortega, Fabricio / Dastmalchi, Maryam / Vencovsky, Jiri / Andersson, Helena / Chinoy, Hector / Lilleker, James B / Shinjo, Samuel Katsuyuki / Maurer, Britta / Griger, Zoltan / Ceribelli, Angela / Torres-Ruiz, Jiram / Mercado M, Vazquez-Del / Leonard, Dag / Alexanderson, Helene / Lundberg, Ingrid E

    Seminars in arthritis and rheumatism

    2024  Volume 65, Page(s) 152379

    Abstract: Aim: To explore if patient global assessment (PGA) is associated with inflammation over time and if associations are explained by other measures of disease activity and function in patients with idiopathic inflammatory myopathies (IIM).: Methods: PGA ...

    Abstract Aim: To explore if patient global assessment (PGA) is associated with inflammation over time and if associations are explained by other measures of disease activity and function in patients with idiopathic inflammatory myopathies (IIM).
    Methods: PGA and systemic inflammatory markers prospectively collected over five years were retrieved from the International MyoNet registry for 1200 patients with IIM. Associations between PGA, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and creatine kinase (CK) were analyzed using mixed models. Mediation analysis was used to test if the association between PGA and inflammatory markers during the first year of observation could be explained by measures of disease activity and function.
    Results: PGA improved, and inflammatory markers decreased during the first year of observation. In the mixed models, high levels of inflammatory markers were associated with worse PGA in both men and women across time points during five years of observation. In men, but not in women, the association between elevated ESR, CRP and poorer PGA was explained by measures of function and disease activity. With a few exceptions, the association between improved PGA and reduced inflammatory markers was partially mediated by improvements in all measures of function and disease activity.
    Conclusion: Increased levels of systemic inflammation are associated with poorer PGA in patients with IIM. In addition to known benefits of lowered inflammation, these findings emphasize the need to reduce systemic inflammation to improve subjective health in patients with IIM. Furthermore, the results demonstrate the importance of incorporating PGA as an outcome measure in clinical practice and clinical trials.
    MeSH term(s) Male ; Humans ; Female ; Longitudinal Studies ; Myositis/complications ; Inflammation ; Outcome Assessment, Health Care ; Blood Sedimentation
    Language English
    Publishing date 2024-01-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 120247-9
    ISSN 1532-866X ; 0049-0172
    ISSN (online) 1532-866X
    ISSN 0049-0172
    DOI 10.1016/j.semarthrit.2024.152379
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Nailfold capillaroscopy findings of a multicentric multi-ethnic cohort of patients with idiopathic inflammatory myopathies.

    Torres-Ruiz, Jiram / Pinal-Fernandez, Iago / Selva-O'Callaghan, Albert / Campbell, Bianca / Muñoz-Braceras, Sandra / Mejía-Domínguez, Nancy R / Núñez-Álvarez, Carlos / Milisenda, José / Casal-Domínguez, Maria / Pak, Katherine / Guillén-Del-Castillo, Alfredo / Trallero-Araguas, Ernesto / Gil-Vila, Albert / Mammen, Andrew Lee

    Clinical and experimental rheumatology

    2024  Volume 42, Issue 2, Page(s) 367–376

    Abstract: Objectives: To assess nailfold video capillaroscopic (NVC) abnormalities and their association with clinical features, myositis-specific autoantibodies (MSA), and myositis-associated antibodies (MAA) in a large multi-ethnic cohort of patients with ... ...

    Abstract Objectives: To assess nailfold video capillaroscopic (NVC) abnormalities and their association with clinical features, myositis-specific autoantibodies (MSA), and myositis-associated antibodies (MAA) in a large multi-ethnic cohort of patients with idiopathic inflammatory myopathies (IIM).
    Methods: We recruited 155 IIM patients from three centres in Mexico, Spain, and the USA. We evaluated the clinical and laboratory features of the patients and performed semiquantitative and quantitative analyses of the NVC. Each NVC study was defined as having a normal, non-specific, early systemic sclerosis (SSc), active SSc, or late SSc pattern. Twenty-three patients had at least one follow-up NVC when disease control was achieved. Quantitative variables were expressed as medians and interquartile range (IQR) and were compared with the Kruskal-Wallis, the Mann-Whitney U-test, and the Wilcoxon test for paired medians. Associations between qualitative variables were assessed with the χ2 test.
    Results: Most patients were women (68.3%), Hispanic (73.5%), and had dermatomyositis (DM) (61.2%). Fourteen patients (9%) had a normal NVC. A non-specific abnormality pattern was the most frequent (53.9%), and was associated with joint involvement, interstitial lung disease, Jo1 autoantibodies, anti-synthetase syndrome, and immune-mediated necrotising myopathy. The SSc pattern was observed mostly in DM and overlap myositis and was associated with cutaneous features and anti-TIF-1g autoantibodies. After treatment, there was a decrease in the capillaroscopic score, the capillary diameter, and the number of avascular areas, and an increase in capillary density and bushy capillary number.
    Conclusions: NVC abnormalities are related to the diagnosis, clinical features, disease activity, and autoantibodies of patients with IIM.
    MeSH term(s) Humans ; Female ; Male ; Microscopic Angioscopy ; Nails/blood supply ; Myositis/complications ; Capillaries ; Autoantibodies ; Scleroderma, Systemic/diagnosis
    Chemical Substances Autoantibodies
    Language English
    Publishing date 2024-03-14
    Publishing country Italy
    Document type Journal Article
    ZDB-ID 605886-3
    ISSN 1593-098X ; 0392-856X
    ISSN (online) 1593-098X
    ISSN 0392-856X
    DOI 10.55563/clinexprheumatol/l9gudh
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Salivary IgA subtypes as novel disease biomarkers in systemic lupus erythematosus.

    Romero-Ramírez, Sandra / Sosa-Hernández, Víctor A / Cervantes-Díaz, Rodrigo / Carrillo-Vázquez, Daniel A / Meza-Sánchez, David E / Núñez-Álvarez, Carlos / Torres-Ruiz, Jiram / Gómez-Martín, Diana / Maravillas-Montero, José L

    Frontiers in immunology

    2023  Volume 14, Page(s) 1080154

    Abstract: Introduction: Immunoglobulin A (IgA) is the main antibody isotype in body fluids such as tears, intestinal mucous, colostrum, and saliva. There are two subtypes of IgA in humans: IgA1, mainly present in blood and mucosal sites, and IgA2, preferentially ... ...

    Abstract Introduction: Immunoglobulin A (IgA) is the main antibody isotype in body fluids such as tears, intestinal mucous, colostrum, and saliva. There are two subtypes of IgA in humans: IgA1, mainly present in blood and mucosal sites, and IgA2, preferentially expressed in mucosal sites like the colon. In clinical practice, immunoglobulins are typically measured in venous or capillary blood; however, alternative samples, including saliva, are now being considered, given their non-invasive and easy collection nature. Several autoimmune diseases have been related to diverse abnormalities in oral mucosal immunity, such as rheumatoid arthritis, Sjogren's syndrome, and systemic lupus erythematosus (SLE).
    Methods: We decided to evaluate the levels of both IgA subtypes in the saliva of SLE patients. A light chain capture-based ELISA measured specific IgA1 and IgA2 levels in a cohort of SLE patients compared with age and gender-matched healthy volunteers.
    Results: Surprisingly, our results indicated that in the saliva of SLE patients, total IgA and IgA1 subtype were significantly elevated; we also found that salivary IgA levels, particularly IgA2, positively correlate with anti-dsDNA IgG antibody titers. Strikingly, we also detected the presence of salivary anti-nucleosome IgA antibodies in SLE patients, a feature not previously reported elsewhere.
    Conclusions: According to our results and upon necessary validation, IgA characterization in saliva could represent a potentially helpful tool in the clinical care of SLE patients with the advantage of being a more straightforward, faster, and safer method than manipulating blood samples.
    MeSH term(s) Humans ; Immunoglobulin A, Secretory ; Lupus Erythematosus, Systemic ; Immunoglobulin A ; Immunoglobulin G ; Mouth Mucosa ; Biomarkers
    Chemical Substances Immunoglobulin A, Secretory ; Immunoglobulin A ; Immunoglobulin G ; Biomarkers
    Language English
    Publishing date 2023-02-22
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2023.1080154
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Mycobacterium tuberculosis

    Pérez-Noriega, Flaubert Alexis / Salinas-Lara, Citlaltepetl / Sánchez-Garibay, Carlos / Torres-Ruíz, José Jiram / Maravillas-Montero, José Luis / Castañón-Arreola, Mauricio / Hernández-Campos, María Elena / Rodríguez-Balderas, Cesar / Basurto-López, Beatriz Victoria / Peñafiel-Salgado, Carlos / Espinosa-García, Ana Paola / Choreño-Parra, José Alberto / Tena-Suck, Martha Lilia / Soto-Rojas, Luis O / León-Marroquín, Elsa Y / Romero-López, José Pablo / Castillejos-López, Manuel

    International journal of molecular sciences

    2023  Volume 24, Issue 2

    Abstract: Tuberculosis (TB) of the central nervous system (CNS) presents high mortality due to brain damage and inflammation events. The formation and deposition of immune complexes (ICs) in the brain microvasculature ... ...

    Abstract Tuberculosis (TB) of the central nervous system (CNS) presents high mortality due to brain damage and inflammation events. The formation and deposition of immune complexes (ICs) in the brain microvasculature during
    MeSH term(s) Male ; Animals ; Mice ; Mycobacterium tuberculosis ; Antigen-Antibody Complex ; Disease Models, Animal ; Tuberculosis/microbiology ; Antigens, Bacterial ; Vasculitis ; Cell Wall
    Chemical Substances Antigen-Antibody Complex ; Antigens, Bacterial
    Language English
    Publishing date 2023-01-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24021242
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  8. Article ; Online: Circulating B10 regulatory cells are decreased in severe and critical COVID-19.

    Cervantes-Díaz, Rodrigo / Sosa-Hernández, Víctor A / Romero-Ramírez, Sandra / Torres-Ruiz, Jiram / Pérez-Fragoso, Alfredo / Meza-Sánchez, David E / Gómez-Martín, Diana / Maravillas-Montero, José L

    Journal of leukocyte biology

    2022  Volume 112, Issue 2, Page(s) 333–337

    Abstract: The contribution of B cells in COVID-19 pathogenesis, beyond the production of specific antibodies against SARS-CoV-2, is still not well understood. Since one of their most relevant functional roles includes their immune-suppressive mechanisms, we ... ...

    Abstract The contribution of B cells in COVID-19 pathogenesis, beyond the production of specific antibodies against SARS-CoV-2, is still not well understood. Since one of their most relevant functional roles includes their immune-suppressive mechanisms, we decided to evaluate one of the most recognized human B regulatory subpopulations: the IL-10
    MeSH term(s) B-Lymphocytes, Regulatory ; COVID-19 ; Flow Cytometry ; Humans ; Interleukin-10 ; SARS-CoV-2
    Chemical Substances Interleukin-10 (130068-27-8)
    Language English
    Publishing date 2022-02-24
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 605722-6
    ISSN 1938-3673 ; 0741-5400
    ISSN (online) 1938-3673
    ISSN 0741-5400
    DOI 10.1002/JLB.5COVCRA0721-387RR
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  9. Article: Pathogenic autoantibody internalization in myositis.

    Pinal-Fernandez, Iago / Muñoz-Braceras, Sandra / Casal-Dominguez, Maria / Pak, Katherine / Torres-Ruiz, Jiram / Musai, Jon / Dell'Orso, Stefania / Naz, Faiza / Islam, Shamima / Gutierrez-Cruz, Gustavo / Cano, Maria Dolores / Matas-Garcia, Ana / Padrosa, Joan / Tobías-Baraja, Esther / Garrabou, Gloria / Aldecoa, Iban / Espinosa, Gerard / Simeon-Aznar, Carmen Pilar / Guillen-Del-Castillo, Alfredo /
    Gil-Vila, Albert / Trallero-Araguas, Ernesto / Christopher-Stine, Lisa / Lloyd, Thomas E / Liewluck, Teerin / Naddaf, Elie / Stenzel, Werner / Greenberg, Steven A / Grau, Josep Maria / Selva-O'Callaghan, Albert / Milisenda, Jose C / Mammen, Andrew L

    medRxiv : the preprint server for health sciences

    2024  

    Abstract: Objectives: Myositis is a heterogeneous family of autoimmune muscle diseases. As myositis autoantibodies recognize intracellular proteins, their role in disease pathogenesis has been unclear. This study aimed to determine whether myositis autoantibodies ...

    Abstract Objectives: Myositis is a heterogeneous family of autoimmune muscle diseases. As myositis autoantibodies recognize intracellular proteins, their role in disease pathogenesis has been unclear. This study aimed to determine whether myositis autoantibodies reach their autoantigen targets within muscle cells and disrupt the normal function of these proteins.
    Methods: Confocal immunofluorescence microscopy was used to localize antibodies and other proteins of interest in myositis muscle biopsies. Bulk RNA sequencing was used to study the transcriptomic profiles of 668 samples from patients with myositis, disease controls, and healthy controls. Antibodies from myositis patients were introduced into cultured myoblasts by electroporation and the transcriptomic profiles of the treated myoblasts were studied by bulk RNA sequencing.
    Results: In patients with myositis autoantibodies, antibodies accumulated inside myofibers in the same subcellular compartment as the autoantigen. Each autoantibody was associated with effects consistent with dysfunction of its autoantigen, such as the derepression of genes normally repressed by Mi2/NuRD in patients with anti-Mi2 autoantibodies, the accumulation of RNAs degraded by the nuclear RNA exosome complex in patients with anti-PM/Scl autoantibodies targeting this complex, and the accumulation of lipids within myofibers of anti-HMGCR-positive patients. Internalization of patient immunoglobulin into cultured myoblasts recapitulated the transcriptomic phenotypes observed in human disease, including the derepression of Mi2/NuRD-regulated genes in anti-Mi2-positive dermatomyositis and the increased expression of genes normally degraded by the nuclear RNA exosome complex in anti-PM/Scl-positive myositis.
    Conclusions: In myositis, autoantibodies are internalized into muscle fibers, disrupt the biological function of their autoantigen, and mediate the pathophysiology of the disease.
    Language English
    Publishing date 2024-01-17
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.01.15.24301339
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  10. Article ; Online: Identification of Unique microRNA Profiles in Different Types of Idiopathic Inflammatory Myopathy.

    Muñoz-Braceras, Sandra / Pinal-Fernandez, Iago / Casal-Dominguez, Maria / Pak, Katherine / Milisenda, José César / Lu, Shajia / Gadina, Massimo / Naz, Faiza / Gutierrez-Cruz, Gustavo / Dell'Orso, Stefania / Torres-Ruiz, Jiram / Grau-Junyent, Josep Maria / Selva-O'Callaghan, Albert / Paik, Julie J / Albayda, Jemima / Christopher-Stine, Lisa / Lloyd, Thomas E / Corse, Andrea M / Mammen, Andrew L

    Cells

    2023  Volume 12, Issue 17

    Abstract: Dermatomyositis (DM), antisynthetase syndrome (AS), immune-mediated necrotizing myopathy (IMNM), and inclusion body myositis (IBM) are four major types of idiopathic inflammatory myopathy (IIM). Muscle biopsies from each type of IIM have unique ... ...

    Abstract Dermatomyositis (DM), antisynthetase syndrome (AS), immune-mediated necrotizing myopathy (IMNM), and inclusion body myositis (IBM) are four major types of idiopathic inflammatory myopathy (IIM). Muscle biopsies from each type of IIM have unique transcriptomic profiles. MicroRNAs (miRNAs) target messenger RNAs (mRNAs), thereby regulating their expression and modulating transcriptomic profiles. In this study, 18 DM, 12 IMNM, 6 AS, 6 IBM, and 6 histologically normal muscle biopsies underwent miRNA profiling using the NanoString nCounter system. Eleven miRNAs were exclusively differentially expressed in DM compared to controls, seven miRNAs were only differentially expressed in AS, and nine miRNAs were specifically upregulated in IBM. No differentially expressed miRNAs were identified in IMNM. We also analyzed miRNA-mRNA associations to identify putative targets of differentially expressed miRNAs. In DM and AS, these were predominantly related to inflammation and cell cycle progression. Moreover, our analysis showed an association between miR-30a-3p, miR-30e-3p, and miR-199b-5p downregulation in DM and the upregulation of target genes induced by type I interferon. In conclusion, we show that muscle biopsies from DM, AS, and IBM patients have unique miRNA signatures and that these miRNAs might play a role in regulating the expression of genes known to be involved in IIM pathogenesis.
    MeSH term(s) Humans ; Myositis/genetics ; MicroRNAs/genetics ; Myositis, Inclusion Body ; Autoimmune Diseases ; RNA, Messenger
    Chemical Substances MicroRNAs ; RNA, Messenger
    Language English
    Publishing date 2023-09-02
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Intramural
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells12172198
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