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Article: Development and Validation of a Capillary Zone Electrophoresis-Tandem Mass Spectrometry Method for Simultaneous Quantification of Eight β-Lactam Antibiotics and Two β-Lactamase Inhibitors in Plasma Samples.

Cizmarova, Ivana / Mikus, Peter / Svidrnoch, Martin / Piestansky, Juraj

2024 Volume 17, Issue 4

Abstract: Monitoring plasma concentrations of β-lactam antibiotics is crucial, particularly in critically ill patients, where variations in concentrations can lead to treatment failure or adverse events. Standardized antimicrobial regimens may not be effective for ...

Abstract	Monitoring plasma concentrations of β-lactam antibiotics is crucial, particularly in critically ill patients, where variations in concentrations can lead to treatment failure or adverse events. Standardized antimicrobial regimens may not be effective for all patients, especially in special groups with altered physiological parameters. Pharmacokinetic/pharmacodynamic (PK/PD) studies highlight the time-dependent antibacterial activity of these antibiotics, emphasizing the need for personalized dosing. Therapeutic drug monitoring (TDM) is essential, requiring rapid and accurate analytical methods for precise determination of drugs in biological material (typically plasma or serum). This study presents a novel capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) method designed for the simultaneous quantification of five penicillin antibiotics, two cephalosporins, one carbapenem, and two β-lactamase inhibitors in a single run. The method involves a simple sample pretreatment-precipitation with organic solvent-and has a run time of 20 min. Optimization of CZE separation conditions revealed that 20 mM ammonium hydrogen carbonate (NH
Language	English
Publishing date	2024-04-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2193542-7
ISSN	1424-8247
ISSN	1424-8247
DOI	10.3390/ph17040526
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Article: Determination of thiamine and pyridoxine in food supplements and beverages by the simple capillary zone electrophoresis in combination with UV detection.

Matušková, Michaela / Čižmárová, Ivana / Mikuš, Peter / Piešťanský, Juraj

Ceska a Slovenska farmacie : casopis Ceske farmaceuticke spolecnosti a Slovenske farmaceuticke spolecnosti

2021 Volume 69, Issue 5-6, Page(s) 237–243

Abstract: ... electrolyte composed of 25 mmol l-1 GABA + 50 mmol l-1 HAc+ 0.05% m-HEC. The UV detector was set ...

Title translation	Stanovenie tiamínu a pyridoxínu vo výživových doplnkoch a nápojoch jednoduchou metódou kapilárnej zónovej elektroforézy v spojení s UV detekciou.
Abstract	The paper is focused on development of a simple analytical method based on capillary zone electro-phoresis in combination with UV-detection for simul-taneous detemination of thiamine and pyridoxine in pharmaceutical and food samples. The separation of thiamine and pyridoxine was performed in a background electrolyte composed of 25 mmol l-1 GABA + 50 mmol l-1 HAc+ 0.05% m-HEC. The UV detector was set at the constant wavelength of 260 nm. Limit of detection was 0.059 µg ml-1 for thiamine and 0.23 µg ml-1 for pyridoxine. These levels suggest that relatively low quantities of thiamine and pyridoxine can be detected. The presented CZE-UV method enabled effective determination of the two vitamins in 5 food supplements and 11 energy drinks and vitamin waters.
MeSH term(s)	Beverages/analysis ; Dietary Supplements ; Electrophoresis, Capillary ; Pyridoxine ; Thiamine/analysis
Chemical Substances	Pyridoxine (KV2JZ1BI6Z) ; Thiamine (X66NSO3N35)
Language	English
Publishing date	2021-01-23
Publishing country	Czech Republic
Document type	Journal Article
ZDB-ID	1187133-7
ISSN	1210-7816 ; 0009-0530
ISSN	1210-7816 ; 0009-0530
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Article ; Online: Tryptophan: Its Metabolism along the Kynurenine, Serotonin, and Indole Pathway in Malignant Melanoma.

Hubková, Beáta / Valko-Rokytovská, Marcela / Čižmárová, Beáta / Zábavníková, Marianna / Mareková, Mária / Birková, Anna

International journal of molecular sciences

2022 Volume 23, Issue 16

Abstract: 1) Background: Tryptophan metabolism is known to be one of the important mechanisms used by cancer to evade immune surveillance. Altered tryptophan metabolism was studied in patients with pigmented malignant melanoma confirmed histologically by the ... ...

Abstract	(1) Background: Tryptophan metabolism is known to be one of the important mechanisms used by cancer to evade immune surveillance. Altered tryptophan metabolism was studied in patients with pigmented malignant melanoma confirmed histologically by the anatomic stage grouping for cutaneous melanoma using clinical staging on the basis of the Breslow thickness of the melanoma, the degree of spread to regional lymph nodes, and by the presence of distant metastasis. (2) Methods: Urinary tryptophan metabolites were detected by RP-HPLC method. (3) Results: In the present work, we provided evidence of altered metabolism of all tryptophan pathways in melanoma patients. (4) Conclusions: Knowledge of the shifted serotonin pathway toward DHICA formation and kynurenine pathway shifted toward NAD
MeSH term(s)	Humans ; Indoles ; Kynurenine/metabolism ; Melanoma ; Serotonin/metabolism ; Skin Neoplasms ; Tryptophan/metabolism ; Melanoma, Cutaneous Malignant
Chemical Substances	Indoles ; Serotonin (333DO1RDJY) ; Kynurenine (343-65-7) ; Tryptophan (8DUH1N11BX)
Language	English
Publishing date	2022-08-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23169160
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Article: Capillary zone electrophoresis in combination with UV detection for simultaneous determination of tramadol and paracetamol in pharmaceutical and biological samples.

Horniaková, Andrea / Štefánik, Ondrej / Čižmárová, Ivana / Matušková, Michaela / Mikuš, Peter / Piešťanský, Juraj

Ceska a Slovenska farmacie : casopis Ceske farmaceuticke spolecnosti a Slovenske farmaceuticke spolecnosti

2022 Volume 71, Issue 2, Page(s) 67–77

Abstract: The aim of the present study is the development and validation of a simple method based on capillary zone electrophoresis coupled with UV detection for simultaneous determination of tramadol and paracetamol in pharmaceutical and biological samples. The ... ...

Title translation	Kapilárna zónová elektroforéza v spojení s UV detekciou pre simultánne stanovenie tramadolu a paracetamolu vo vzorkách farmaceutického a biologického charakteru.
Abstract	The aim of the present study is the development and validation of a simple method based on capillary zone electrophoresis coupled with UV detection for simultaneous determination of tramadol and paracetamol in pharmaceutical and biological samples. The background electrolyte was composed of 50 mM ammonium carbonate, which is a type of a non-conventional electrolyte system. The developed method was characterized by suitable validation parameters, such as linearity (coefficient of determination r2 0,995), selectivity or the limit of detection at the level of 0.25 - 0.5 μg/ml. Acceptable values of accuracy and precision were obtained, which were in good agreement with the recommended validation guidelines for analysis of pharmaceutical and biological samples. Detection was performed at a wavelength of 200 nm. The developed method was successfully applied to determine tramadol and paracetamol in various dosage forms and in urine biological samples. Achieved results indicate a potential of the method to be integrated in the common quality control processes of drugs and/or in bioanalysis.
MeSH term(s)	Acetaminophen ; Electrophoresis, Capillary/methods ; Pharmaceutical Preparations ; Tramadol/analysis
Chemical Substances	Pharmaceutical Preparations ; Acetaminophen (362O9ITL9D) ; Tramadol (39J1LGJ30J)
Language	English
Publishing date	2022-06-21
Publishing country	Czech Republic
Document type	Journal Article
ZDB-ID	1187133-7
ISSN	1210-7816 ; 0009-0530
ISSN	1210-7816 ; 0009-0530
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Article: Capillary Electrophoresis-Mass Spectrometry with Multisegment Injection and In-Capillary Preconcentration for High-Throughput and Sensitive Determination of Therapeutic Decapeptide Triptorelin in Pharmaceutical and Biological Matrices.

Piešťanský, Juraj / Čižmárová, Ivana / Štefánik, Ondrej / Matušková, Michaela / Horniaková, Andrea / Majerová, Petra / Mikuš, Peter

Biomedicines

2021 Volume 9, Issue 10

Abstract: ... of the analyzed peptide onto the capillary wall (1 M formic acid-HFo, pH 1.88). The implementation of the field ...

Abstract	A capillary electrophoresis-tandem mass spectrometry method with a multisegment injection and an in-capillary field-enhanced sample stacking for determination of therapeutic peptide triptorelin in pharmaceutical and biological matrices was developed. The CE separation conditions were optimized in order to obtain maximal separation efficiency, analytical signal intensity and stability, and minimal adsorption of the analyzed peptide onto the capillary wall (1 M formic acid-HFo, pH 1.88). The implementation of the field-enhanced sample injection into CE improved the value of limit of detection 50 times while the multisegment injection increased the sample throughput three times in comparison to a conventional CE approach. The proposed method was characterized by favorable performance parameters, such as linearity (r
Language	English
Publishing date	2021-10-16
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines9101488
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Article ; Online: The Effect of Oestrogen Supplementation on Antioxidant Enzymes and Mitochondrial Respiratory Function After Myocardial Infarction of Ovariectomized Rats.

Čižmárová, Beáta / Evinová, Andrea / Račay, Peter / Birková, Anna / Hubková, Beáta / Mareková, Mária / Toporcer, Tomáš / Grendel, Tomáš / Toporcerová, Silva / Vašková, Janka

Journal of cardiovascular pharmacology

2022 Volume 79, Issue 5, Page(s) 663–669

Abstract: Abstract: Acute myocardial infarction (MI) is the leading cause of mortality worldwide with premenopausal women showing a lower incidence of cardiovascular disease compared with men of the same age. After menopause, this advantage disappears, suggesting ...

Abstract	Abstract: Acute myocardial infarction (MI) is the leading cause of mortality worldwide with premenopausal women showing a lower incidence of cardiovascular disease compared with men of the same age. After menopause, this advantage disappears, suggesting that sex hormones play a cardioprotective role. This study was aimed to assess on the activity of antioxidant enzymes in plasma and the respiratory function of isolated heart mitochondria after the induction of MI in rats after ovariectomy and estradiol benzoate supplementation. Sprague-Dawley female rats were ovariectomized 3 months before the induction of MI and supplemented/not supplemented with oestrogen 3 months before/7 days after the induction of MI. No significant differences in glutathione peroxidase activities were found in any group. Differences between values were only significant in the ovariectomized not supplemented group (P < 0.01) for the glutathione reductase activity and glutathione concentrations. In isolated mitochondria (7 days after MI), the decline in respiration was observed comparing the ovariectomized and nonovariectomized group. Respiratory functions did not show significant differences between animals supplemented with oestrogen before MI or treated with oestrogen after MI. Ovariectomy worsened mitochondrial dysfunction after MI, and oestrogen supplementation before or after the induction of MI did not improve mitochondrial function.
MeSH term(s)	Animals ; Antioxidants/pharmacology ; Dietary Supplements ; Estradiol/pharmacology ; Estrogens ; Female ; Humans ; Mitochondria ; Myocardial Infarction/prevention & control ; Ovariectomy ; Rats ; Rats, Sprague-Dawley ; Respiration
Chemical Substances	Antioxidants ; Estrogens ; Estradiol (4TI98Z838E)
Language	English
Publishing date	2022-05-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	391970-5
ISSN	1533-4023 ; 0160-2446
ISSN (online)	1533-4023
ISSN	0160-2446
DOI	10.1097/FJC.0000000000001224
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Article ; Online: Comparison of 1D a 2D ITP-MS performance parameters and application possibilities: Ultratrace determination of B vitamins in human urine.

Piestansky, Juraj / Cizmarova, Ivana / Matuskova, Michaela / Mikus, Peter

Electrophoresis

2021 Volume 43, Issue 9-10, Page(s) 998–1009

Abstract: The possibility to investigate analytes at ultra-low concentration levels still remains a hot topic in bioanalysis. In this area, various preconcentration techniques are an integral part of analytical procedures. When applying electromigration separation ...

Abstract	The possibility to investigate analytes at ultra-low concentration levels still remains a hot topic in bioanalysis. In this area, various preconcentration techniques are an integral part of analytical procedures. When applying electromigration separation techniques, an isotachophoresis has been advantageously employed many times for this purpose. To solve current biomedical tasks effectively, an advanced two-dimensional isotachophoretic instrument (in a hydrodynamically closed separation system with an enhanced sample load capacity) hyphenated with mass spectrometry (ITP-ITP-MS) has been proposed by Foret and coworkers. As a continuation, this work represents the first study dealing with a full validation of an ITP-ITP-MS method. In order to see the benefits of an online ITP sample pretreatment (preconcentration and clean-up) on the performance parameters, the developed 2D ITP-MS method was compared with a corresponding 1D ITP-MS method. Application potentialities of the compared methods were demonstrated via a determination of two B vitamins, namely thiamine and pyridoxine, in human urine samples. The developed 2D ITP-MS method showed its enhanced effectivity and usefulness for a routine biomedical use (here, a reliable screening of trace B vitamins in human urine without an offline sample preparation).
MeSH term(s)	Electrophoresis, Capillary/methods ; Humans ; Isotachophoresis/methods ; Mass Spectrometry ; Vitamin B Complex
Chemical Substances	Vitamin B Complex (12001-76-2)
Language	English
Publishing date	2021-10-12
Publishing country	Germany
Document type	Journal Article
ZDB-ID	619001-7
ISSN	1522-2683 ; 0173-0835
ISSN (online)	1522-2683
ISSN	0173-0835
DOI	10.1002/elps.202100209
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Article ; Online: Determination of Branched-Chain Amino Acids in Food Supplements and Human Plasma by a CE-MS/MS Method with Enhanced Resolution.

Piestansky, Juraj / Matuskova, Michaela / Cizmarova, Ivana / Olesova, Dominika / Mikus, Peter

International journal of molecular sciences

2021 Volume 22, Issue 15

Abstract: In the presented study, a capillary electrophoresis-mass spectrometry method combining high separation efficiency and sensitive detection has been developed and validated, for the first time, to quantify branched chain amino acids (valine, isoleucine, ... ...

Abstract	In the presented study, a capillary electrophoresis-mass spectrometry method combining high separation efficiency and sensitive detection has been developed and validated, for the first time, to quantify branched chain amino acids (valine, isoleucine, leucine) in commercial food and sport supplement samples and human plasma samples. The separations were performed in a bare fused silica capillary. The background electrolyte was composed of 500 mM formic acid with pH 2.0. The plasma sample pretreatment was realized by simple protein precipitation with acetonitrile. Injection of a short zone of highly basic electrolyte before the sample injection and application of the negative pressure on the separation were accompanied by enhanced resolution of the isobaric amino acids-isoleucine and leucine. The developed method was characterized by favorable validation parameters, such as linearity (r
MeSH term(s)	Adult ; Amino Acids, Branched-Chain/analysis ; Amino Acids, Branched-Chain/blood ; Blood/metabolism ; Blood Chemical Analysis/methods ; Dietary Supplements ; Electrophoresis, Capillary/methods ; Humans ; Male ; Mass Spectrometry/methods
Chemical Substances	Amino Acids, Branched-Chain
Language	English
Publishing date	2021-07-31
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22158261
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Article ; Online: Successful correction of hyperglycemia is critical for weight loss and a decrease in cardiovascular risk in obese patients.

Zalejska-Fiolka, Jolanta / Birková, Anna / Hubková, Beáta / Wielkoszyński, Tomasz / Čižmárová, Beáta / Szlachta, Beata / Fiolka, Rafał / Błaszczyk, Urszula / Wylęgała, Adam / Kasperczyk, Sławomir / Grzanka, Alicja / Mareková, Mária / Toborek, Michal

The Journal of nutritional biochemistry

2022 Volume 106, Page(s) 109021

Abstract: ... hyperglycemic patients. Obese (body mass index30 kg/m ...

Abstract	Weight loss is recommended for obese patients with cardiovascular risk; however, it remains questionable how hyperglycemia affects this process. To address this problem, we aimed to determine the association between weight loss, lipid profile, and body mass parameters in obese normoglycemic and hyperglycemic patients. Obese (body mass index30 kg/m
MeSH term(s)	Atherosclerosis ; Body Mass Index ; Cardiovascular Diseases/etiology ; Cardiovascular Diseases/prevention & control ; Glucose ; Heart Disease Risk Factors ; Humans ; Hyperglycemia/complications ; Insulin Resistance/physiology ; Lipids ; Obesity/metabolism ; Risk Factors ; Weight Loss
Chemical Substances	Lipids ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2022-04-25
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1014929-6
ISSN	1873-4847 ; 0955-2863
ISSN (online)	1873-4847
ISSN	0955-2863
DOI	10.1016/j.jnutbio.2022.109021
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Zs.A 2838: Show issues

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Article ; Online: Capillary Electrophoresis–Mass Spectrometry with Multisegment Injection and In-Capillary Preconcentration for High-Throughput and Sensitive Determination of Therapeutic Decapeptide Triptorelin in Pharmaceutical and Biological Matrices

Juraj Piešťanský / Ivana Čižmárová / Ondrej Štefánik / Michaela Matušková / Andrea Horniaková / Petra Majerová / Peter Mikuš

Biomedicines, Vol 9, Iss 1488, p

2021 Volume 1488

Abstract: ... of the analyzed peptide onto the capillary wall (1 M formic acid—HFo, pH 1.88). The implementation of the field ...

Abstract	A capillary electrophoresis–tandem mass spectrometry method with a multisegment injection and an in-capillary field-enhanced sample stacking for determination of therapeutic peptide triptorelin in pharmaceutical and biological matrices was developed. The CE separation conditions were optimized in order to obtain maximal separation efficiency, analytical signal intensity and stability, and minimal adsorption of the analyzed peptide onto the capillary wall (1 M formic acid—HFo, pH 1.88). The implementation of the field-enhanced sample injection into CE improved the value of limit of detection 50 times while the multisegment injection increased the sample throughput three times in comparison to a conventional CE approach. The proposed method was characterized by favorable performance parameters, such as linearity (r 2 ≥ 0.99), limit of detection (5 ng mL −1 in water matrix, 25 ng mL −1 in plasma matrix), precision (relative standard deviation, 1.5–9.4% for intraday and 2.3–11.9% for interday reproducibility), or accuracy (relative errors in the range of 80–109%). The FDA-validated method was successfully applied to the analysis of triptorelin in the commercial drug Diphereline ® 0.1 mg (powder for injection) and in spiked human plasma samples. Favorable performance parameters along with proven application potentialities indicate the usefulness of the proposed method for its routine use in drug quality control laboratories and for clinical analysis, such as determination of triptorelin levels in plasma (for pharmacokinetic study).
Keywords	capillary electrophoresis ; tandem mass spectrometry ; triptorelin peptide drug ; field-enhanced sample injection ; multisegment injection ; pharmaceutical and biological samples ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2021-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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