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  1. Article ; Online: Spermatozoal RNAs: what about their functions?

    Dadoune, Jean-Pierre

    Microscopy research and technique

    2009  Volume 72, Issue 8, Page(s) 536–551

    Abstract: The profound architectural changes that transform spermatids into spermatozoa result in a high degree of DNA packaging within the sperm head. However, the mature sperm chromatin that harbors imprinted genes exhibits a dual nucleoprotamine/nucleohistone ... ...

    Abstract The profound architectural changes that transform spermatids into spermatozoa result in a high degree of DNA packaging within the sperm head. However, the mature sperm chromatin that harbors imprinted genes exhibits a dual nucleoprotamine/nucleohistone structure with DNase-sensitive regions, which could be implicated in the establishment of efficient epigenetic information in the developing embryo. Despite its apparent transcriptionally inert state, the sperm nucleus contains diverse RNA populations, mRNAs, antisense and miRNAs, that have been transcribed throughout spermatogenesis. There is also an endogenous reverse transcriptase that may be activated under certain circumstances. It is now commonly accepted that sperm can deliver some RNAs to the ovocyte at fertilization. This review presents potential links between male-specific genomic imprinting, chromatin organization, and the presence of diverse RNA populations within the sperm nucleus and discusses the functional significance of these RNAs in the spermatozoon itself and in the early embryo following fertilization. Some recent data are provided, supporting the view that analyzing the profile of spermatozoal RNAs could be useful for assessment of male fertility.
    MeSH term(s) Animals ; Genomic Imprinting ; Humans ; Male ; MicroRNAs/physiology ; RNA, Antisense/physiology ; RNA, Messenger/physiology ; Spermatozoa/physiology
    Chemical Substances MicroRNAs ; RNA, Antisense ; RNA, Messenger
    Language English
    Publishing date 2009-08
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 1099714-3
    ISSN 1097-0029 ; 1059-910X
    ISSN (online) 1097-0029
    ISSN 1059-910X
    DOI 10.1002/jemt.20697
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Book: Histoire ordinaire et extraordinaire des cellules sexuelles

    Dadoune, Jean-Pierre

    (Histoire des sciences)

    2011  

    Author's details Jean-Pierre Dadoune ; avec la participation de Philippe Lherminier, Martin Catala ; postfaces de Jean-Jacques Marimbert, Pierre Ancet ; les dessins ont été réalisés par Madame Sophie Gournet
    Series title Histoire des sciences
    MeSH term(s) Stem Cells ; Stem Cell Research
    Language French
    Size 245 p. :, ill. ;, 22 cm.
    Publisher Hermann
    Publishing place Paris
    Document type Book
    ISBN 9782705680022 ; 2705680020
    Database Catalogue of the US National Library of Medicine (NLM)

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  3. Article: New insights into male gametogenesis: what about the spermatogonial stem cell niche?

    Dadoune, Jean-Pierre

    Folia histochemica et cytobiologica

    2007  Volume 45, Issue 3, Page(s) 141–147

    Abstract: The spermatogonial stem cells (SSCs) are at the foundation of spermatogenesis. They can self-renew and generate a large number of differentiated germ cells. A balance between SSC renewal and differentiation in the adult testis is essential to maintain ... ...

    Abstract The spermatogonial stem cells (SSCs) are at the foundation of spermatogenesis. They can self-renew and generate a large number of differentiated germ cells. A balance between SSC renewal and differentiation in the adult testis is essential to maintain normal spermatogenesis and fertility. These two processes are tightly regulated by intrinsic gene expression in the stem cells and extrinsic signals, including soluble factors and adhesion molecules from the surrounding microenvironment, known as the SSC niche. Few factors which are only found in germ cells are recognized as indispensable to SSC maintenance or differentiation. Plzf, a transcriptional repressor protein, and TAF-4b, a germ cell specific component of the RNA polymerase complex, are considered to be essential for SSC renewal, whereas the transcription factors Sohlh1 and 2 appear to be crucial for spermatogonial differentiation. By providing glial cell-line-derived neurotrophic factor (GDNF) and stem cell factor (SCF) for SSCs and segregating them, the Sertoli cell is one of the major contributors to stem cell niche regulation Another Sertoli cell product, ERM, a transcription factor which is exclusively expressed in mature Sertoli cells, has been shown to be required for SSC self-renewal and maintenance of spermatogenesis in adult mice. It has been hypothesized that SSC niche regulation changes with age. SSC self-renewal could be controlled by GDNF during the perinatal period of development while it is dependent on ERM, during the pubertal period.
    MeSH term(s) Animals ; Cell Differentiation/physiology ; Gametogenesis ; Humans ; Male ; Mice ; Models, Biological ; Spermatogonia/cytology ; Spermatogonia/growth & development ; Stem Cells/cytology
    Language English
    Publishing date 2007
    Publishing country Poland
    Document type Journal Article ; Review
    ZDB-ID 605761-5
    ISSN 1897-5631 ; 0239-8508 ; 0015-5586
    ISSN (online) 1897-5631
    ISSN 0239-8508 ; 0015-5586
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Expression of mammalian spermatozoal nucleoproteins.

    Dadoune, Jean-Pierre

    Microscopy research and technique

    2003  Volume 61, Issue 1, Page(s) 56–75

    Abstract: A dramatic remodeling of sperm chromatin occurs during mammalian spermiogenesis. Nuclear elongation and chromatin condensation are concomitant with modifications in the basic protein complement associated with DNA. A number of biochemical events ... ...

    Abstract A dramatic remodeling of sperm chromatin occurs during mammalian spermiogenesis. Nuclear elongation and chromatin condensation are concomitant with modifications in the basic protein complement associated with DNA. A number of biochemical events accompany the displacement of histones and the appearance of protamines in elongating spermatids. The mRNAs of transition proteins and protamines are transcribed and stored in the cytoplasm of spermatids until days later when they are translated. The intrinsic regulation of the expression of the transition protein and protamine genes occurs at three levels: transcription, translation, and posttranslation. The aim of this review is to cover most of the morphological, biochemical, and functional events which concern nuclear protein transitions during spermiogenesis and which are thereby involved in the nuclear status of ejaculated sperm cells.
    MeSH term(s) Animals ; Cricetinae ; Dogs ; Gene Expression Regulation ; Guinea Pigs ; Haploidy ; Humans ; Male ; Mice ; Nucleoproteins/genetics ; Nucleoproteins/metabolism ; Protamines/genetics ; Protamines/metabolism ; Protein Biosynthesis ; Proteins/genetics ; Proteins/metabolism ; Rabbits ; Rats ; Spermatids/ultrastructure ; Spermatogenesis/physiology ; Spermatozoa/metabolism ; Transcription, Genetic
    Chemical Substances Nucleoproteins ; Protamines ; Proteins
    Language English
    Publishing date 2003-05-01
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 1099714-3
    ISSN 1097-0029 ; 1059-910X
    ISSN (online) 1097-0029
    ISSN 1059-910X
    DOI 10.1002/jemt.10317
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: New insights into male gametogenesis

    Jean-Pierre Dadoune

    Folia Histochemica et Cytobiologica, Vol 45, Iss 3, Pp 141-

    what about the spermatogonial stem cell niche?

    2007  Volume 147

    Abstract: The spermatogonial stem cells (SSCs) are at the foundation of spermatogenesis. They can self-renew and generate a large number of differentiated germ cells. A balance between SSC renewal and differentiation in the adult testis is essential to maintain ... ...

    Abstract The spermatogonial stem cells (SSCs) are at the foundation of spermatogenesis. They can self-renew and generate a large number of differentiated germ cells. A balance between SSC renewal and differentiation in the adult testis is essential to maintain normal spermatogenesis and fertility. These two processes are tightly regulated by intrinsic gene expression in the stem cells and extrinsic signals, including soluble factors and adhesion molecules from the surrounding microenvironment, known as the SSC niche. Few factors which are only found in germ cells are recognized as indispensable to SSC maintenance or differentiation. Plzf, a transcriptional repressor protein, and TAF-4b, a germ cell specific component of the RNA polymerase complex, are considered to be essential for SSC renewal, whereas the transcription factors Sohlh1 and 2 appear to be crucial for spermatogonial differentiation. By providing glial cell-line-derived neurotrophic factor (GDNF) and stem cell factor (SCF) for SSCs and segregating them, the Sertoli cell is one of the major contributors to stem cell niche regulation Another Sertoli cell product, ERM, a transcription factor which is exclusively expressed in mature Sertoli cells, has been shown to be required for SSC self-renewal and maintenance of spermatogenesis in adult mice. It has been hypothesized that SSC niche regulation changes with age. SSC self-renewal could be controlled by GDNF during the perinatal period of development while it is dependent on ERM, during the pubertal period.
    Keywords Biology (General) ; QH301-705.5 ; Science ; Q ; DOAJ:Biology ; DOAJ:Biology and Life Sciences
    Subject code 571
    Language English
    Publishing date 2007-10-01T00:00:00Z
    Publisher Polish Histochemical and Cytochemical Society
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Transcription in haploid male germ cells.

    Dadoune, Jean-Pierre / Siffroi, Jean-Pierre / Alfonsi, Marie-Françoise

    International review of cytology

    2004  Volume 237, Page(s) 1–56

    Abstract: Major modifications in chromatin organization occur in spermatid nuclei, resulting in a high degree of DNA packaging within the spermatozoon head. However, before arrest of transcription during midspermiogenesis, high levels of mRNA are found in round ... ...

    Abstract Major modifications in chromatin organization occur in spermatid nuclei, resulting in a high degree of DNA packaging within the spermatozoon head. However, before arrest of transcription during midspermiogenesis, high levels of mRNA are found in round spermatids. Some transcripts are the product of genes expressed ubiquitously, whereas some are generated from male germ cell-specific gene homologs of somatic cell genes. Others are transcript variants derived from genes with expression regulated in a testis-specific fashion. The haploid genome of spermatids also initiates the transcription of testis-specific genes. Various general transcription factors, distinct promoter elements, and specific transcription factors are involved in transcriptional regulation. After meiosis, spermatids are genetically but not phenotypically different, because of transcript and protein sharing through cytoplasmic bridges connecting spermatids of the same generation. Interestingly, different types of mRNAs accumulate in the sperm cell nucleus, raising the question of their origin and of a possible role after fertilization.
    MeSH term(s) Animals ; Cell Nucleus/genetics ; Cell Nucleus/metabolism ; Cell Nucleus/ultrastructure ; Gene Expression Regulation, Developmental/genetics ; Genes, Regulator/genetics ; Haploidy ; Humans ; Male ; Meiosis/genetics ; RNA, Messenger/genetics ; Spermatids/growth & development ; Spermatids/metabolism ; Spermatids/ultrastructure ; Spermatogenesis/genetics ; Spermatozoa/growth & development ; Spermatozoa/metabolism ; Spermatozoa/ultrastructure ; Transcription, Genetic/genetics
    Chemical Substances RNA, Messenger
    Language English
    Publishing date 2004
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 209869-6
    ISSN 0074-7696
    ISSN 0074-7696
    DOI 10.1016/S0074-7696(04)37001-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Collecting Human Spermatozoa onto Filters for FISH

    Siffroi, Jean Pierre / Le Bourhis, Corine / Dadoune, Jean Pierre

    Acta Cytologica

    2011  Volume 46, Issue 6, Page(s) 1123–1128

    Institution From the Department of Histology, Biology of Reproduction and Cytogenetics, Tenon Hospital, Paris, France
    Language English
    Publishing date 2011-03-09
    Publisher S. Karger AG
    Publishing place Basel, Switzerland
    Document type Article
    Note Molecular Techniques
    ZDB-ID 80003-x
    ISSN 1938-2650 ; 0001-5547
    ISSN (online) 1938-2650
    ISSN 0001-5547
    DOI 10.1159/000327118
    Database Karger publisher's database

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  8. Book: Histologie

    Dadoune, Jean-Pierre

    (Collection De la biologie à la clinique,)

    1990  

    Author's details Jean-Piere Dadoune ... [et al.]
    Series title Collection De la biologie à la clinique,
    MeSH term(s) Histology
    Language French
    Size x, 462 p. :, ill.
    Publisher Flammarion médecine-sciences
    Publishing place Paris
    Document type Book
    ISBN 9782257101204 ; 2257101200
    Database Catalogue of the US National Library of Medicine (NLM)

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  9. Article: Collecting human spermatozoa onto filters for FISH. Application to the study of extreme oligozoospermia.

    Siffroi, Jean Pierre / Le Bourhis, Corine / Dadoune, Jean Pierre

    Acta cytologica

    2002  Volume 46, Issue 6, Page(s) 1123–1128

    Abstract: Objective: To describe a simple method of fluorescence in situ hybridization (FISH) performed on germ cells collected on filters that allows the study of aneuploid sperm frequency in cases of extreme oligozoospermia.: Study design: The aneuploidy ... ...

    Abstract Objective: To describe a simple method of fluorescence in situ hybridization (FISH) performed on germ cells collected on filters that allows the study of aneuploid sperm frequency in cases of extreme oligozoospermia.
    Study design: The aneuploidy rates for chromosomes X, Y and 18 were analyzed in three groups to test the reliability of the filtration-FISH procedure when compared to controls and to evaluate the frequency of aneuploid spermatozoa in five oligospermic men.
    Results: Filtration of semen samples before FISH does not modify the basal rate of aneuploidy even after various sperm dilutions. In oligospermic men, the frequency of gonosomal aneuploidies is significantly increased.
    Conclusion: The filtration-FISH technique is a powerful method of analyzing chromosomal abnormalities in sperm nuclei in men with extreme oligozoospermia.
    MeSH term(s) Cell Nucleus/pathology ; Cell Separation/methods ; Chromosome Aberrations ; Humans ; In Situ Hybridization, Fluorescence/methods ; Male ; Oligospermia/genetics ; Oligospermia/pathology ; Spermatozoa/pathology
    Language English
    Publishing date 2002-11
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80003-x
    ISSN 1938-2650 ; 0001-5547
    ISSN (online) 1938-2650
    ISSN 0001-5547
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Impaired gap junction connexin43 in Sertoli cells of patients with secretory azoospermia: a marker of undifferentiated Sertoli cells.

    Defamie, Norah / Berthaut, Isabelle / Mograbi, Baharia / Chevallier, Daniel / Dadoune, Jean-Pierre / Fénichel, Patrick / Segretain, Dominique / Pointis, Georges

    Laboratory investigation; a journal of technical methods and pathology

    2003  Volume 83, Issue 3, Page(s) 449–456

    Abstract: Gap junctions are intercellular channels formed of connexins (Cx) at appositional plasma membranes between adjacent cells that have been involved in the control of cell proliferation and differentiation. Altered Cx expression is implicated consistently ... ...

    Abstract Gap junctions are intercellular channels formed of connexins (Cx) at appositional plasma membranes between adjacent cells that have been involved in the control of cell proliferation and differentiation. Altered Cx expression is implicated consistently in several human diseases and in tumorigenesis. Although Cx43 plays a critical role in Sertoli cell control of spermatogenesis, there is no evidence of its altered expression in human testicular pathologies. We show here that Cx43 mRNA expression was significantly reduced in testes of infertile patients with secretory azoospermia (p < 0.05) compared with testes displaying normal spermatogenesis (excretory azoospermic patients). In Sertoli cell-only syndrome, in situ hybridization and immunohistochemistry analyses indicated that Cx43 mRNA and protein were undetectable in Sertoli cells but were still present in the interstitial compartment. In a rat model of Sertoli cell-only syndrome, the lack of Cx43 in Sertoli cells was associated with an impairment of gap junction intercellular communication between adjacent Sertoli cells. These results reveal that Cx43 mRNA and protein expression are markedly impaired in Sertoli cells of infertile patients. This defect could be a new functional marker of undifferentiated Sertoli cells and could be related to the increased risk of testicular cancer recently described in the population of infertile men.
    MeSH term(s) Adult ; Aged ; Animals ; Biomarkers ; Connexin 43/genetics ; Connexin 43/metabolism ; Humans ; Immunoenzyme Techniques ; In Situ Hybridization ; Male ; Middle Aged ; Oligospermia/metabolism ; Oligospermia/pathology ; RNA, Messenger/metabolism ; Rats ; Rats, Long-Evans ; Reverse Transcriptase Polymerase Chain Reaction ; Sertoli Cells/metabolism ; Staining and Labeling
    Chemical Substances Biomarkers ; Connexin 43 ; RNA, Messenger
    Language English
    Publishing date 2003-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80178-1
    ISSN 1530-0307 ; 0023-6837
    ISSN (online) 1530-0307
    ISSN 0023-6837
    DOI 10.1097/01.lab.0000059928.82702.6d
    Database MEDical Literature Analysis and Retrieval System OnLINE

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