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Article ; Online: Transportal Balloon-Occluded Retrograde Transvenous Obliteration for a Pancreatic Arteriovenous Malformation Using the Outflow Debranching Technique.

Jogo, Atsushi / Yamamoto, Akira / Mukai, Kaori / Kageyama, Ken / Kaminou, Toshio / Miki, Yukio

Journal of vascular and interventional radiology : JVIR

2023 Volume 34, Issue 5, Page(s) 927–931

MeSH term(s)	Humans ; Balloon Occlusion/methods ; Sclerosing Solutions/therapeutic use ; Arteriovenous Malformations/complications ; Arteriovenous Malformations/diagnostic imaging ; Arteriovenous Malformations/therapy ; Esophageal and Gastric Varices
Chemical Substances	Sclerosing Solutions
Language	English
Publishing date	2023-01-02
Publishing country	United States
Document type	Letter
ZDB-ID	1137756-2
ISSN	1535-7732 ; 1051-0443
ISSN (online)	1535-7732
ISSN	1051-0443
DOI	10.1016/j.jvir.2022.12.477
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Zs.A 3720: Show issues

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Article ; Online: Dysphagia in patients with severe COVID-19: a retrospective study.

Asayama, Rie / Tanaka-Nishikubo, Kaori / Okada, Masahiro / Mukai, Naoki / Annen, Suguru / Matsumoto, Hironori / Takeba, Jun / Sato, Norio / Hato, Naohito

Scientific reports

2024 Volume 14, Issue 1, Page(s) 6829

Abstract: To investigate dysphagia after extubation in patients with severe coronavirus disease 2019 (COVID-19). We retrospectively examined patients with severe COVID-19 treated in our hospital between August 2021 and March 2022. Feeding outcomes were categorized ...

Abstract	To investigate dysphagia after extubation in patients with severe coronavirus disease 2019 (COVID-19). We retrospectively examined patients with severe COVID-19 treated in our hospital between August 2021 and March 2022. Feeding outcomes were categorized into two groups-(1) total oral intake, and (2) difficulty in oral intake. To assess the feeding outcome, we used modified water-swallowing test (MWST) for all patients. However, in cases where aspiration or recurrent laryngeal nerve palsy was suspected, we conducted the fiberoptic endoscopic evaluation of swallowing after MWST. Patient data were collected from medical records. Forty-six patients with severe COVID-19 were included. Among the 46 patients, 14 (30.4%) experienced difficulties with oral intake. Older age, longer length of hospitalization, duration of mechanical ventilation, tracheostomy, diabetes, and higher serum levels of C-reactive protein (CRP) and procalcitonin (PCT) at the time of intubation were associated with difficulty in oral intake. The rate of difficulty with oral intake in patients with severe COVID-19 was 30.4%, which is not as high as reported in previous studies. Older age, longer duration of mechanical ventilation, tracheostomy, diabetes, and higher levels of CRP and PCT were associated with the prevalence of oral intake difficulty, suggesting that early attention should be paid to high-risk patients who have preexisting deterioration of swallowing function due to aging and comorbidities, or who have prolonged intubation or tracheostomy to prevent aspiration pneumonia.
MeSH term(s)	Humans ; COVID-19/complications ; Retrospective Studies ; Deglutition Disorders ; Deglutition ; Diabetes Mellitus
Language	English
Publishing date	2024-03-21
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-57508-x
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Article ; Online: Microfabrication of Gelatin Methacrylate/Hydroxyapatite Composites by Utilizing Alternate Soaking Process.

Miyajima, Hiroki / Kojima, Kaori / Touji, Hiroki / Onodera, Kodai / Mukai, Masaru / Maruo, Shoji / Iijima, Kazutoshi

ACS biomaterials science & engineering

2023 Volume 10, Issue 2, Page(s) 762–772

Abstract: To construct a complex three-dimensional (3D) structure mimicking bone microstructure, hydrogel models of polymerized gelatin methacrylate (pGelMA) were fabricated by using stereolithography and modified with hydroxyapatite (HAp) via an alternate soaking ...

Abstract	To construct a complex three-dimensional (3D) structure mimicking bone microstructure, hydrogel models of polymerized gelatin methacrylate (pGelMA) were fabricated by using stereolithography and modified with hydroxyapatite (HAp) via an alternate soaking process (ASP) using a solution of calcium and phosphate ions. Fabricated pGelMA line models whose widths were designed as 100, 300, and 600 μm were modified with HAp by ASP by changing the immersion time and number of cycles. After ASP, all of the line models with widths of 100, 300, and 600 μm were successfully modified with HAp, and large amounts of HAp were covered with the fabricated models by increasing both the immersion time and the number of cycles in ASP. HAp was observed near the surface of the line model with a width of 600 μm after ASP at an immersion time of 10 s, while the entire model was modified with HAp using ASPs for longer immersion times. The adhesion and spread of mesenchymal stem cells (MSCs) on the pGelMA-HAp discs depended on the ASP conditions. Moreover, the HAp modification of 3D pyramid models without alteration of the microstructure was also conducted. This two-step fabrication method of first fabricating frameworks of hydrogel models by stereolithography and subsequently modifying the fabricated models with HAp will lead to the development of 3D cell culture systems to support bone grafts or to create biological niches, such as artificial bone marrow.
MeSH term(s)	Durapatite/chemistry ; Gelatin/chemistry ; Microtechnology ; Bone and Bones ; Hydrogels
Chemical Substances	Durapatite (91D9GV0Z28) ; Gelatin (9000-70-8) ; Hydrogels
Language	English
Publishing date	2023-11-20
Publishing country	United States
Document type	Journal Article
ISSN	2373-9878
ISSN (online)	2373-9878
DOI	10.1021/acsbiomaterials.3c01046
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Article: Portal Vein Aneurysm in a Patient with Cirrhosis Type C Controlled by Direct-Acting Antiviral Treatment.

Higashi, Sena / Nakabori, Tasuku / Mukai, Kaori / Seiki, Yusuke / Watsuji, Ko / Hirao, Takeru / Kawamoto, Yasuharu / Urabe, Makiko / Kai, Yugo / Takada, Ryoji / Yamai, Takuo / Ikezawa, Kenji / Uehara, Hiroyuki / Ohkawa, Kazuyoshi

Case reports in gastroenterology

2024 Volume 18, Issue 1, Page(s) 74–80

Abstract: Introduction: Portal vein aneurysm (PVA) is a rare saccular or fusiform portal vein dilatation. The management and optimal treatment of PVA remain unknown.: Case presentation: A 53-year-old man with hepatitis C virus (HCV) infection was diagnosed ... ...

Abstract	Introduction: Portal vein aneurysm (PVA) is a rare saccular or fusiform portal vein dilatation. The management and optimal treatment of PVA remain unknown. Case presentation: A 53-year-old man with hepatitis C virus (HCV) infection was diagnosed with PVA measuring 28 mm in diameter. Under observation, his liver fibrosis progressed, and the PVA diameter gradually increased to 52 mm. The patient was treated with elbasvir-grazoprevir for 12 weeks, and HCV disappeared. After achieving sustained virological response, liver fibrosis improved and the PVA progression ceased. Conclusion: HCV clearance by direct-acting antiviral treatment not only regressed liver fibrosis but may have also restrained the progression of PVA in a patient with cirrhosis type C and PVA.
Language	English
Publishing date	2024-02-16
Publishing country	Switzerland
Document type	Case Reports
ZDB-ID	2440540-1
ISSN	1662-0631
ISSN	1662-0631
DOI	10.1159/000535957
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Article ; Online: Safety and Feasibility of Combining On-Demand Selective Locoregional Treatment with First-Line Atezolizumab Plus Bevacizumab for Patients with Unresectable Hepatocellular Carcinoma.

Nakabori, Tasuku / Higashi, Sena / Abe, Yutaro / Mukai, Kaori / Ikawa, Toshiki / Konishi, Koji / Maeda, Noboru / Nakanishi, Katsuyuki / Hasegawa, Shinichiro / Wada, Hiroshi / Ohkawa, Kazuyoshi

Current oncology (Toronto, Ont.)

2024 Volume 31, Issue 3, Page(s) 1543–1555

Abstract: Various locoregional treatments for localized hepatocellular carcinoma (HCC) have been developed. This retrospective study investigated the safety and feasibility of combining on-demand selective locoregional treatment for residual lesions after tumor ... ...

Abstract	Various locoregional treatments for localized hepatocellular carcinoma (HCC) have been developed. This retrospective study investigated the safety and feasibility of combining on-demand selective locoregional treatment for residual lesions after tumor shrinkage (complete response [CR] oriented) or for solitary or few drug-resistant lesions (progressive disease (PD) salvage) with first-line atezolizumab plus bevacizumab (atezo/bev) for unresectable HCC. Twenty-nine patients with unresectable HCC were included. Fourteen locoregional treatments were performed (CR oriented, 7; PD salvage, 7) in ten patients in the combination-therapy group. All patients in the combination-therapy group successfully achieved a CR or PD salvage status after the planned locoregional treatment. The objective response rate of the combination-therapy group (80.0%) was higher than that of the atezo/bev alone group (21.1%;
MeSH term(s)	Humans ; Carcinoma, Hepatocellular ; Bevacizumab ; Feasibility Studies ; Retrospective Studies ; Liver Neoplasms ; Antibodies, Monoclonal, Humanized
Chemical Substances	Bevacizumab (2S9ZZM9Q9V) ; atezolizumab (52CMI0WC3Y) ; Antibodies, Monoclonal, Humanized
Language	English
Publishing date	2024-03-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1236972-x
ISSN	1718-7729 ; 1198-0052
ISSN (online)	1718-7729
ISSN	1198-0052
DOI	10.3390/curroncol31030117
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Zs.A 4305: Show issues

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bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Development of a radioiodinated thioflavin-T-Congo-red hybrid probe for diagnosis of systemic amyloidosis.

Haratake, Yoshie / Sano, Kohei / Tsuchiya, Miki / Minaki, Kaori / Munekane, Masayuki / Yamasaki, Toshihide / Hagimori, Masayori / Mukai, Takahiro

Bioorganic & medicinal chemistry

2022 Volume 56, Page(s) 116591

Abstract: Introduction: Systemic amyloidosis is a group of diseases characterized by the deposition of amyloid protein in multiple organs throughout the body and causing their dysfunction. As amyloid deposition is observed at an early phase and is highly specific ...

Abstract	Introduction: Systemic amyloidosis is a group of diseases characterized by the deposition of amyloid protein in multiple organs throughout the body and causing their dysfunction. As amyloid deposition is observed at an early phase and is highly specific to systemic amyloidosis, noninvasive detection of amyloid is considered useful for the early diagnosis of systemic amyloidosis. In this study, we designed and synthesized a novel radiolabeled amyloid imaging probe, sodium (E)-4-amino-3-((4-(6-iodobenzothiazol-2-yl)phenyl)diazenyl)naphthalene-1-sulfonate (1), which combines two amyloid-binding compounds, thioflavin-T and Congo-red, and evaluated its effectiveness in diagnosing amyloidosis. Methods: A tributyltin precursor was synthesized through a 5-step reaction from 2-amino-6-bromobenzothiazole, and [ Results: [ Conclusion: These results indicate the potential of radioiodinated 1 as a nuclear imaging probe for diagnosing AA amyloidosis.
MeSH term(s)	Amyloidosis/diagnosis ; Animals ; Autoradiography ; Benzothiazoles/chemistry ; Congo Red/chemistry ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Drug Development ; Female ; Iodine Radioisotopes ; Mice ; Mice, Inbred ICR ; Molecular Structure ; Radiopharmaceuticals/chemical synthesis ; Radiopharmaceuticals/chemistry ; Structure-Activity Relationship
Chemical Substances	Benzothiazoles ; Iodine Radioisotopes ; Radiopharmaceuticals ; thioflavin T (2390-54-7) ; Congo Red (3U05FHG59S) ; Iodine-125 (GVO776611R)
Language	English
Publishing date	2022-01-08
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1161284-8
ISSN	1464-3391 ; 0968-0896
ISSN (online)	1464-3391
ISSN	0968-0896
DOI	10.1016/j.bmc.2021.116591
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Zs.A 3815: Show issues

Location:
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Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Usefulness of on-site cytology of liver tumor biopsy in specimen sampling for cancer genomic profiling test.

Nakabori, Tasuku / Abe, Yutaro / Higashi, Sena / Mukai, Kaori / Yoshioka, Risa / Morimoto, Yuki / Koyanagi, Yuki / Tanada, Satoshi / Nagata, Shigenori / Honma, Keiichiro / Ohkawa, Kazuyoshi

Cancer medicine

2023 Volume 12, Issue 7, Page(s) 7888–7892

Abstract: Aim: Appropriate sample selection with a tumor fraction ≥20% without necrosis contamination is required for successful cancer genomic profiling (CGP). Rapid on-site evaluation (ROSE) is performed to assess adequate sampling.: Method: This ... ...

Abstract	Aim: Appropriate sample selection with a tumor fraction ≥20% without necrosis contamination is required for successful cancer genomic profiling (CGP). Rapid on-site evaluation (ROSE) is performed to assess adequate sampling. Method: This retrospective study included 54 patients who underwent CGP using liver tumor biopsy specimen with ROSE. Result: The sampling success rate (98.1%) was higher than the previously reported 77.5%-88.9%. ROSE was performed once in 51 patients and twice in three patients; for those undergoing ROSE twice, the first ROSE was negative for malignancy, or showed few tumor cells with necrotic cell contamination, while the second ROSE obtained from another location showed abundant malignant cells. In these patients, the CGP was successful using the second specimen, though the first sample did not meet the required criteria for CGP test. Conclusion: Performing ROSE during liver tumor biopsy may be useful for CGP test sampling because ROSE prevents sampling errors and contributes to adequate sampling.
MeSH term(s)	Humans ; Retrospective Studies ; Biopsy ; Cytodiagnosis ; Liver Neoplasms/diagnosis ; Liver Neoplasms/genetics ; Genomics
Language	English
Publishing date	2023-01-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	2659751-2
ISSN	2045-7634 ; 2045-7634
ISSN (online)	2045-7634
ISSN	2045-7634
DOI	10.1002/cam4.5563
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Article ; Online: Sensitivity of cytology in liver tumor biopsy and its significance in the prompt clinical diagnosis of non-hepatocellular carcinoma.

Nakabori, Tasuku / Abe, Yutaro / Higashi, Sena / Mukai, Kaori / Shingetsu, Azusa / Nishimura, Sanako / Tsuzaki, Sayoko / Ryu, Ayumi / Tanada, Satoshi / Nagata, Shigenori / Honma, Keiichiro / Ohkawa, Kazuyoshi

Cancer medicine

2023 Volume 12, Issue 11, Page(s) 12336–12342

Abstract: Background: Cytology is a fast and simple modality for identifying malignancies and tumor histology. In this study, we analyzed the sensitivity of cytology for liver tumor biopsy and evaluated its potential for prompt clinical diagnosis.: Methods: ... ...

Abstract	Background: Cytology is a fast and simple modality for identifying malignancies and tumor histology. In this study, we analyzed the sensitivity of cytology for liver tumor biopsy and evaluated its potential for prompt clinical diagnosis. Methods: This retrospective study included patients who had concurrently undergone conventional cytology, on-site cytology, and histopathology for ultrasound-guided liver tumor biopsies. In the case of malignant tumors, malignancy was first diagnosed, then preliminary clinical diagnosis was established using histology based on cytology and clinical information, followed by histopathological diagnosis. Sensitivity of malignancy detection was evaluated by comparison with histopathological diagnosis. Results: Of the 191 tumors, 164 (85.9%) were malignant. The sensitivity of conventional cytology for malignancy detection was 97.6%. The sensitivity of non-hepatocellular carcinoma (non-HCC) (99.3%) detection was higher than that of the HCCs (87.5%; p = 0.001). The sensitivity of on-site cytology for malignancy detection was as high as that of conventional cytology. Similar to conventional cytology, the sensitivity of on-site cytology for non-HCC detection (99.3%) was higher than that for HCCs (79.2%; p < 0.001). In most cases of non-HCC tumors (126/140, 90.0%), accurate preliminary clinical diagnoses were obtained by combining on-site cytology with clinical information. Conclusion: Cytology of liver tumor biopsy has high sensitivity for malignancy, especially in non-HCC tumors. On-site cytology can contribute to the prompt clinical diagnosis of non-HCC tumors when combined with clinical information. This approach may be a reassuring modality for patients with severely advanced cancers requiring prompt clinical diagnosis and quick initiation of treatment owing to their deteriorating health.
MeSH term(s)	Humans ; Retrospective Studies ; Liver Neoplasms/diagnosis ; Liver Neoplasms/pathology ; Biopsy ; Cytodiagnosis ; Image-Guided Biopsy ; Carcinoma/pathology ; Sensitivity and Specificity ; Carcinoma, Hepatocellular/diagnosis ; Carcinoma, Hepatocellular/pathology
Language	English
Publishing date	2023-04-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	2659751-2
ISSN	2045-7634 ; 2045-7634
ISSN (online)	2045-7634
ISSN	2045-7634
DOI	10.1002/cam4.5934
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Article ; Online: The role of Sp140 revealed in IgE and mast cell responses in Collaborative Cross mice.

Matsushita, Kazufumi / Li, Xin / Nakamura, Yuki / Dong, Danyue / Mukai, Kaori / Tsai, Mindy / Montgomery, Stephen B / Galli, Stephen J

JCI insight

2021 Volume 6, Issue 12

Abstract: Mouse IgE and mast cell (MC) functions have been studied primarily using inbred strains. Here, we (a) identified effects of genetic background on mouse IgE and MC phenotypes, (b) defined the suitability of various strains for studying IgE and MC ... ...

Abstract	Mouse IgE and mast cell (MC) functions have been studied primarily using inbred strains. Here, we (a) identified effects of genetic background on mouse IgE and MC phenotypes, (b) defined the suitability of various strains for studying IgE and MC functions, and (c) began to study potentially novel genes involved in such functions. We screened 47 Collaborative Cross (CC) strains, as well as C57BL/6J and BALB/cJ mice, for strength of passive cutaneous anaphylaxis (PCA) and responses to the intestinal parasite Strongyloides venezuelensis (S.v.). CC mice exhibited a diversity in PCA strength and S.v. responses. Among strains tested, C57BL/6J and CC027 mice showed, respectively, moderate and uniquely potent MC activity. Quantitative trait locus analysis and RNA sequencing of BM-derived cultured MCs (BMCMCs) from CC027 mice suggested Sp140 as a candidate gene for MC activation. siRNA-mediated knock-down of Sp140 in BMCMCs decreased IgE-dependent histamine release and cytokine production. Our results demonstrated marked variations in IgE and MC activity in vivo, and in responses to S.v., across CC strains. C57BL/6J and CC027 represent useful models for studying MC functions. Additionally, we identified Sp140 as a gene that contributes to IgE-dependent MC activation.
MeSH term(s)	Animals ; Antigens, Nuclear/genetics ; Antigens, Nuclear/immunology ; Collaborative Cross Mice ; Female ; Immunoglobulin E/genetics ; Immunoglobulin E/immunology ; Mast Cells/immunology ; Mast Cells/metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Rats ; Rats, Wistar ; Transcription Factors/genetics ; Transcription Factors/immunology
Chemical Substances	Antigens, Nuclear ; Transcription Factors ; Immunoglobulin E (37341-29-0)
Language	English
Publishing date	2021-06-22
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	2379-3708
ISSN (online)	2379-3708
DOI	10.1172/jci.insight.146572
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Article ; Online: An optimized protocol for phenotyping human granulocytes by mass cytometry.

Vivanco Gonzalez, Nora / Oliveria, John-Paul / Tebaykin, Dmitry / Ivison, Geoffrey T / Mukai, Kaori / Tsai, Mindy M / Borges, Luciene / Nadeau, Kari C / Galli, Stephen J / Tsai, Albert G / Bendall, Sean C

STAR protocols

2022 Volume 3, Issue 2, Page(s) 101280

Abstract: Granulocytes encompass diverse roles, from fighting off pathogens to regulating inflammatory processes in allergies. These roles are represented by distinct cellular phenotypes that we captured with mass cytometry (CyTOF). Our protocol enables ... ...

Abstract	Granulocytes encompass diverse roles, from fighting off pathogens to regulating inflammatory processes in allergies. These roles are represented by distinct cellular phenotypes that we captured with mass cytometry (CyTOF). Our protocol enables simultaneous evaluation of human basophils, eosinophils, and neutrophils under homeostasis and upon immune activation by anti-Immunoglobulin E (anti-IgE) or interleukin-3 (IL-3). Granulocyte integrity and detection of protein markers were optimized so that rare granulocyte populations could be deeply characterized by single cell mass cytometry. For complete details on the use and execution of this protocol, please refer to Vivanco Gonzalez et al. (2020).
MeSH term(s)	Basophils ; Eosinophils/metabolism ; Flow Cytometry/methods ; Humans ; Leukocyte Count ; Neutrophils
Language	English
Publishing date	2022-04-08
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	2666-1667
ISSN (online)	2666-1667
DOI	10.1016/j.xpro.2022.101280
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