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  1. Article ; Online: A H

    Hartwig, Stefanie / Dragomirova, Nadya / Kublik, Anja / Türkowsky, Dominique / von Bergen, Martin / Lechner, Ute / Adrian, Lorenz / Sawers, R Gary

    Environmental microbiology reports

    2017  Volume 9, Issue 5, Page(s) 618–625

    Abstract: ... CBDB1 that catalysed H ...

    Abstract Dehalococcoides mccartyi is a small, slow-growing bacterium of the phylum Chloroflexi that conserves energy using aliphatic and aromatic organohalides as electron acceptors, and hydrogen as sole electron donor. A recent study identified a protein complex in the membrane of strain CBDB1 comprising a Hup hydrogenase, a complex iron-sulphur molybdoprotein and a reductive dehalogenase (RdhA) that catalyses reduction of 1,2,3,4-tetrachlorobenzene. Using a combination of size-exclusion chromatography, in-gel hydrogenase activity-staining, immunological analysis and mass spectrometry, we identified here a large molecular mass protein complex solubilized from the cytoplasmic membrane of D. mccartyi strain CBDB1 that catalysed H
    Language English
    Publishing date 2017-10
    Publishing country United States
    Document type Journal Article
    ISSN 1758-2229
    ISSN (online) 1758-2229
    DOI 10.1111/1758-2229.12560
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Double semen collection at a 1-h interval in dogs decreases the bacterial contamination of canine ejaculates.

    Lechner, Dominik / Aurich, Jörg / Spergser, Joachim / Aurich, Christine

    Theriogenology

    2023  Volume 208, Page(s) 126–131

    Abstract: ... the same dogs (n = 28) were submitted to dual semen collection 1 h apart. All ejaculates were submitted ...

    Abstract Semen extenders usually contain antibiotics with the aim to minimize bacterial growth, but the indiscriminate use of antibiotics increases the emergence of multidrug-resistant bacteria. A limiting factor of semen processing in dogs is the low total sperm count that limits the number of insemination doses that can be obtained from one ejaculate. Therefore, two ejaculates collected at a short interval can be combined to increase the number of AI doses. In this study, semen was collected from dogs either once or the same dogs (n = 28) were submitted to dual semen collection 1 h apart. All ejaculates were submitted to bacteriological analysis. We hypothesized that bacterial contamination of semen is low but that a dual semen collection might increase contamination. A sample for bacteriological examination was taken from raw semen immediately after semen collection. Bacteria including mycoplasmas were isolated using conventional cultivation procedures and isolates were identified to the species level by matrix-assisted laser desorption ionization - time of flight (MALDI-ToF) mass spectrometry. In total, 22 bacterial species were identified in the 84 ejaculates with Mycoplasma cynos, Streptococcus canis and Canicola haemoglobinophilus being most frequent. Bacterial growth was sporadic in 16 and absent in 10 ejaculates. The overall bacterial growth was lower in the second than in the first ejaculate of dual semen collections (p < 0.05). The percentage of motile and membrane-intact spermatozoa in frozen-thawed ejaculates was not associated with the degree of bacterial contamination of raw semen. In conclusion, there was only limited microbial contamination in dog semen and the microorganisms isolated are considered part of the normal genital bacterial flora. Repeated semen collection reduced bacterial contamination in the second in comparison to the first ejaculate. The use of antibiotics in canine semen should be questioned.
    MeSH term(s) Male ; Dogs ; Animals ; Semen ; Bacteria ; Spermatozoa ; Anti-Bacterial Agents ; Body Fluids
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2023-06-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 189232-0
    ISSN 1879-3231 ; 0093-691X
    ISSN (online) 1879-3231
    ISSN 0093-691X
    DOI 10.1016/j.theriogenology.2023.06.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Author Response: Chemical Considerations Regarding the H-Value Methodology and Its Relation With Toxicity Determination.

    Menz, Dirk-Henning / Feltgen, Nicolas / Lechner, Thorsten / Menz, Helge / Mueller, Bernd-Kristof / Dresp, Joachim / Hoerauf, Hans

    Investigative ophthalmology & visual science

    2020  Volume 60, Issue 10, Page(s) 3365–3367

    MeSH term(s) Eye Diseases ; Fluorocarbons ; Humans
    Chemical Substances Fluorocarbons ; perfluorooctane (6P60ZBK0QL)
    Language English
    Publishing date 2020-01-07
    Publishing country United States
    Document type Letter ; Comment
    ZDB-ID 391794-0
    ISSN 1552-5783 ; 0146-0404
    ISSN (online) 1552-5783
    ISSN 0146-0404
    DOI 10.1167/iovs.19-27341
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: H. pylori infection increases levels of exhaled nitrate.

    Lechner, Matthias / Karlseder, Alban / Niederseer, David / Lirk, Philipp / Neher, Andreas / Rieder, Josef / Tilg, Herbert

    Helicobacter

    2005  Volume 10, Issue 5, Page(s) 385–390

    Abstract: ... worldwide. Despite the existence of a breath test for the diagnosis of H. pylori infection, no study has ... of patients with H. pylori infection.: Materials and methods: The volatile compounds in the exhaled air ... of 14 patients suffering from H. pylori gastritis and 11 controls were analyzed using proton transfer ...

    Abstract Background: Helicobacter pylori infection is one of the most common chronic bacterial infections worldwide. Despite the existence of a breath test for the diagnosis of H. pylori infection, no study has described the composition of volatile compounds, especially the levels of nitrate, in the exhaled air of patients with H. pylori infection.
    Materials and methods: The volatile compounds in the exhaled air of 14 patients suffering from H. pylori gastritis and 11 controls were analyzed using proton transfer reaction-mass spectrometry. Gastric biopsy was used to establish diagnosis of current H. pylori infection.
    Results: Comparing mass spectra between groups, Mass 28 (hydrogen cyanide, HCN) and Mass 64 (hydrogen nitrate, H2NO3) were found to be significantly elevated in patients with H. pylori infection.
    Conclusions: The main result of the present study is that in H. pylori-infected patients, levels of exhaled hydrogen nitrate and hydrogen cyanide are found to be significantly elevated. However, further studies are necessary to find out whether the differences in the detected mass spectrum are specific enough to differentiate patients with H. pylori gastritis from healthy controls.
    MeSH term(s) Adult ; Breath Tests/methods ; Exhalation/physiology ; Gastritis/diagnosis ; Gastritis/microbiology ; Gastritis/physiopathology ; Helicobacter Infections/diagnosis ; Helicobacter Infections/microbiology ; Helicobacter Infections/physiopathology ; Helicobacter pylori/pathogenicity ; Humans ; Hydrogen Cyanide/metabolism ; Mass Spectrometry/methods ; Middle Aged ; Nitrates/metabolism ; Nitric Oxide Synthase/metabolism ; Nitric Oxide Synthase Type II
    Chemical Substances Nitrates ; Hydrogen Cyanide (2WTB3V159F) ; NOS2 protein, human (EC 1.14.13.39) ; Nitric Oxide Synthase (EC 1.14.13.39) ; Nitric Oxide Synthase Type II (EC 1.14.13.39)
    Language English
    Publishing date 2005-10
    Publishing country England
    Document type Clinical Trial ; Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1330665-0
    ISSN 1523-5378 ; 1083-4389
    ISSN (online) 1523-5378
    ISSN 1083-4389
    DOI 10.1111/j.1523-5378.2005.00345.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Influence of Local Defects on the Dynamics of O–H Bond Breaking and Formation on a Magnetite Surface

    Bourgund, Alexander / Esch, Friedrich / Franchini, Cesare / Heiz, Ueli / Lechner, Barbara A. J / Meier, Matthias / Parkinson, Gareth S

    Journal of physical chemistry. 2019 July 17, v. 123, no. 32

    2019  

    Abstract: The transport of H adatoms across oxide supports plays an important role in many catalytic ... reactions. We investigate the dynamics of H/Fe3O4(001) between 295 and 382 K ... By scanning tunneling microscopy at frame rates of up to 19.6 fps, we observe the thermally activated switching of H between two O ...

    Abstract The transport of H adatoms across oxide supports plays an important role in many catalytic reactions. We investigate the dynamics of H/Fe3O4(001) between 295 and 382 K. By scanning tunneling microscopy at frame rates of up to 19.6 fps, we observe the thermally activated switching of H between two O atoms on neighboring Fe rows. This switching rate changes in proximity to a defect, explained by density functional theory as a distortion in the Fe–O lattice shortening the diffusion path. Quantitative analysis yields an apparent activation barrier of 0.94 ± 0.07 eV on a pristine surface. The present work highlights the importance of local techniques in the study of atomic-scale dynamics at defective surfaces such as oxide supports.
    Keywords catalytic activity ; chemical bonding ; density functional theory ; iron ; magnetite ; quantitative analysis ; scanning tunneling microscopy
    Language English
    Dates of publication 2019-0717
    Size p. 19742-19747.
    Publishing place American Chemical Society
    Document type Article
    ISSN 1932-7455
    DOI 10.1021/acs.jpcc.9b05547
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Benetton vs. Inditex and H&M

    Lechner, Christian / Dowling, Michael / Rigamonti, Eric

    Fallstudien zum Internationalen Management , p. 169-191

    the European fashion battle reloaded

    2008  , Page(s) 169–191

    Author's details Christian Lechner, Eric Rigamonti and Michael Dowling
    Language English
    Publisher Gabler
    Publishing place Wiesbaden
    Document type Article
    Database ECONomics Information System

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  7. Book ; Thesis: Förderung sozial-emotionaler Kompetenzen und Prävention von emotionalen und Verhaltensstörungen. Entwicklung und Evaluation eines Präventionsprogramms für die Grundschule

    Lechner, Viola

    2023  

    Abstract: ... 10.3233/DEV-220335 (3) Lechner, V., Ortelbach, N. & Scheithauer, H. (n. d.). Papilio-6bis9 ... V., Cr&aciun, I. C. & Scheithauer, H. (n. d.). Barriers, resources, and attitudes towards (cyber ... in einem Ausblick für zukünftige Forschung und praktische/gesellschaftliche Veränderungen. - Contents: (1) Lechner ...

    Title translation Promotion of social-emotional competencies and prevention of emotional and behavioral disorders. Development and evaluation of a prevention program for elementary school (DeepL)
    Abstract Die vorliegende kumulative Dissertation beinhaltet drei Manuskripte und zielt darauf ab, unter Berücksichtigung des Wissensstandes der Implementationsforschung, ein universell-selektives schulisches Präventionsprogramm, aufbauend auf den Erkenntnissen der entwicklungsorientierten Prävention und Positiven Psychologie, zu entwickeln. Das Programm Papilio-6bis9 für Kinder der ersten und zweiten Jahrgangsstufe soll den Übergang von einem eher informellen, spielerischen Setting (der Kindertagesstätte) in ein strukturierteres, akademisches Setting (die Grundschule) erleichtern und durch die Förderung sozial-emotionaler Kompetenzen das Risiko für emotionale und Verhaltensprobleme reduzieren. Obwohl das Bewusstsein für evidenzbasierte schulische Prävention und Intervention zunimmt, gibt es einen Mangel an flächendeckender Implementation in der Praxis. Die erste Studie befasst sich mit der Frage, welche Barrieren und welche vorliegenden sowie erwünschten Ressourcen hinsichtlich der Implementation von schulischen Präventions- und Interventionsangeboten (hier bezogen auf (Cyber-)Bullying) vom Schulpersonal wahrgenommen werden und ob sich das Schulpersonal anhand der Daten in bestimmte Typologien/Einstellungsmuster zuordnen lässt. Mit Hilfe eines teilstrukturierten Interviewleitfadens konnten drei partizipative Fokusgruppendiskussionen durchgeführt werden (N = 21, 71% Frauen). Die Transkripte der Fokusgruppen wurden mittels einer datenbasierten, induktiven thematischen Inhaltsanalyse mit MAXQDA 2022 ausgewertet, wobei Codes generiert und anschließend übergeordneten Themen zugeordnet wurden. Die gewonnenen Daten liefern Hinweise auf vier Einstellungstypen (der reaktive Typ, der kollaborative Typ, der intendierende Typ und der initiative Typ), anhand derer praktische Implikationen für die Programmgestaltung und Entwicklung von individualisierbaren Fortbildungsangeboten abgeleitet werden konnten. Das zweite Manuskript zielt darauf ab, ein wissenschaftliches, theoretisch fundiertes und transparentes Vorgehen bei der Programmentwicklung und - resultierend aus den Ergebnissen der Prozessevaluation - der Adaption der Materialien zu beschreiben. Hierzu wurde mit Hilfe des Intervention Mapping Ansatzes (IMA) eine Serie von sechs Schritten durchlaufen: nach einer Bedarfsanalyse und der Definition des Problems, der Ursachen und der Zielgruppe wurde ein Modell der Veränderung inklusive Erläuterung der Programmziele eingeführt. Anschließend folgte eine Auswahl von evidenzbasierten Strategien und die Entwicklung von geeigneten, altersangemessenen Methoden, welche in Form eines manualisierten Programms organisiert und erprobt wurden. Nach der praktischen Testung und Überarbeitung der Methoden wurde das Programm entwickelt, gefolgt von einem Implementations- und Evaluationsplan. Basierend auf einer Stichprobe von 224 Schüler*innen der ersten Klasse (52% Mädchen, Alter M = 7.1 Jahre) wurde dann im Rahmen einer Pilotierung mit Hilfe eines kontrollierten (Wartekontroll-)Gruppen-Längsschnittdesigns mit drei Messzeitpunkten untersucht, ob die Programmteilnahme zu signifikanten Veränderungen in den Bereichen sozial-emotionale Kompetenzen, emotionale und Verhaltensprobleme, Exekutivfunktionen und der Lehrkraft-Schüler*innen-Beziehung führt. Die Ergebnisse konnten in der Interventionsgruppe positive Veränderungen in allen untersuchten Bereichen zeigen und weisen auf die generelle Programmwirksamkeit hin. Abschließend werden theoretische und praktische Implikationen sowie Limitationen erörtert und resultieren in einem Ausblick für zukünftige Forschung und praktische/gesellschaftliche Veränderungen. - Contents: (1) Lechner, V., Cr&aciun, I. C. & Scheithauer, H. (n. d.). Barriers, resources, and attitudes towards (cyber-)bullying prevention and intervention in schools from the perspective of school staff: Practical implications resulting from focus group discussions. Manuscript submitted for publication. (2) https://doi.org/10.3233/DEV-220335 (3) Lechner, V., Ortelbach, N. & Scheithauer, H. (n. d.). Papilio-6bis9 - Förderung sozialemotionaler Kompetenzen und universelle Prävention von emotionalen und Verhaltensproblemen in der Grundschule: Ergebnisse einer Pilotevaluation. Manuscript submitted for publication.
    Keywords Behavior Problems ; Childhood Development ; Emotional Development ; Emotional Regulation ; Emotionale Entwicklung ; Emotionsregulation ; Entwicklung in der Kindheit ; Executive Function ; Exekutive Funktion ; Lehrkraft-Lernende-Interaktion ; Lehrkräfte ; Prevention ; Program Development ; Programmentwicklung ; Prävention ; Psychosocial Development ; Psychosoziale Entwicklung ; School Based Intervention ; Schulische Intervention ; Social Skills ; Soziale Fertigkeiten ; Teacher Student Interaction ; Teachers ; Verhaltensprobleme
    Language German ; English
    Size 95 S.
    Publisher Freie Universität, Fachbereich Erziehungswissenschaft und Psychologie, Arbeitsbereich Entwicklungswissenschaft und Angewandte Entwicklungspsychologie
    Publishing place Berlin
    Document type Book ; Thesis
    Note Kumulative Dissertation
    DOI 10.17169/refubium-38073
    Database PSYNDEX

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  8. Article: A path model of the effects of spirituality on depressive symptoms and 24-h urinary-free cortisol in HIV-positive persons.

    Carrico, Adam W / Ironson, Gail / Antoni, Michael H / Lechner, Suzanne C / Durán, Ron E / Kumar, Mahendra / Schneiderman, Neil

    Journal of psychosomatic research

    2006  Volume 61, Issue 1, Page(s) 51–58

    Abstract: ... reappraisal coping, and benefit finding as they relate to depressive symptoms and 24-h urinary-free-cortisol ... positive men and women on highly active antiretroviral therapy provided 24-h urine samples and completed ... uniquely predictive of decreased 24-h urinary-free cortisol output.: Conclusion: Positive reappraisal ...

    Abstract Objective: The present investigation examined the associations among spirituality, positive reappraisal coping, and benefit finding as they relate to depressive symptoms and 24-h urinary-free-cortisol output.
    Methods: Following an initial screening appointment, 264 human-immunodeficiency-virus-positive men and women on highly active antiretroviral therapy provided 24-h urine samples and completed a battery of psychosocial measures.
    Results: Spirituality was associated with higher positive reappraisal coping and greater benefit finding. Benefit finding and positive reappraisal coping scores were, in turn, both related to lower depressive symptoms. Finally, we determined that benefit finding was uniquely predictive of decreased 24-h urinary-free cortisol output.
    Conclusion: Positive reappraisal coping and benefit finding may co-mediate the effect of spirituality on depressive symptoms, and benefit finding may uniquely explain the effect of spirituality on 24-h cortisol output.
    MeSH term(s) Adaptation, Psychological ; Adult ; Antiretroviral Therapy, Highly Active ; Arousal/physiology ; Depression/psychology ; Depression/urine ; Female ; HIV Seropositivity/drug therapy ; HIV Seropositivity/psychology ; HIV Seropositivity/urine ; Homosexuality, Male/psychology ; Humans ; Hydrocortisone/blood ; Male ; Middle Aged ; Models, Psychological ; Personality Inventory ; Psychometrics ; Randomized Controlled Trials as Topic ; Religion and Psychology ; Spirituality
    Chemical Substances Hydrocortisone (WI4X0X7BPJ)
    Language English
    Publishing date 2006-07
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 80166-5
    ISSN 1879-1360 ; 0022-3999
    ISSN (online) 1879-1360
    ISSN 0022-3999
    DOI 10.1016/j.jpsychores.2006.04.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Open access publishing and the future of scientific communication.

    Hawkes, Christopher H / Giovannoni, Gavin / Lechner-Scott, Jeanette / Levy, Michael / Yeh, Ann

    Multiple sclerosis and related disorders

    2024  Volume 86, Page(s) 105625

    Language English
    Publishing date 2024-04-15
    Publishing country Netherlands
    Document type Editorial
    ZDB-ID 2645330-7
    ISSN 2211-0356 ; 2211-0348
    ISSN (online) 2211-0356
    ISSN 2211-0348
    DOI 10.1016/j.msard.2024.105625
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Click, Compute, Create: A Review of Web-based Tools for Enzyme Engineering.

    Tripp, Adrian / Braun, Markus / Wieser, Florian / Oberdorfer, Gustav / Lechner, Horst

    Chembiochem : a European journal of chemical biology

    2024  , Page(s) e202400092

    Abstract: Enzyme engineering, though pivotal across various biotechnological domains, is often plagued by its time-consuming and labor-intensive nature. This review aims to offer an overview of supportive in silico methodologies for this demanding endeavor. ... ...

    Abstract Enzyme engineering, though pivotal across various biotechnological domains, is often plagued by its time-consuming and labor-intensive nature. This review aims to offer an overview of supportive in silico methodologies for this demanding endeavor. Starting from methods to predict protein structures, to classification of their activity and even the discovery of new enzymes we continue with describing tools used to increase thermostability and production yields of selected targets. Subsequently, we discuss computational methods to modulate both, the activity as well as selectivity of enzymes. Last, we present recent approaches based on cutting-edge machine learning methods to redesign enzymes. With exception of the last chapter, there is a strong focus on methods easily accessible via web-interfaces or simple Python-scripts, therefore readily useable for a diverse and broad community.
    Language English
    Publishing date 2024-04-18
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2020469-3
    ISSN 1439-7633 ; 1439-4227
    ISSN (online) 1439-7633
    ISSN 1439-4227
    DOI 10.1002/cbic.202400092
    Database MEDical Literature Analysis and Retrieval System OnLINE

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