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  1. Article ; Online: Complete genome sequence of seven virulent Newcastle disease virus isolates of sub-genotype XIII.1.1 from Tanzania.

    Goraichuk, Iryna V / Msoffe, Peter L M / Chiwanga, Gaspar H / Dimitrov, Kiril M / Afonso, Claudio L / Suarez, David L

    Microbiology resource announcements

    2023  Volume 12, Issue 10, Page(s) e0040523

    Abstract: We report the complete genome sequences of seven virulent Newcastle disease viruses (NDVs) that were isolated from chickens from live bird markets in the Arusha, Iringa, Mbeya, and Tanga regions of Tanzania in 2012. Phylogenetic analysis revealed that ... ...

    Abstract We report the complete genome sequences of seven virulent Newcastle disease viruses (NDVs) that were isolated from chickens from live bird markets in the Arusha, Iringa, Mbeya, and Tanga regions of Tanzania in 2012. Phylogenetic analysis revealed that all isolates belong to sub-genotype XIII.1.1.
    Language English
    Publishing date 2023-09-26
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/MRA.00405-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Intranasal booster vaccination of beef steers reduces clinical signs following experimental coinfection with BRSV and BHV-1 without reducing shedding of BRD-associated bacteria.

    Martínez, David A / Chamorro, Manuel F / Passler, Thomas / Huber, Laura / Falkenberg, Shollie / Walz, Paul H / Thoresen, Merrilee / Raithel, Gage / Silvis, Scott / Dimitrov, Kiril M / Stockler, Ricardo / Woolums, Amelia R

    American journal of veterinary research

    2024  Volume 85, Issue 5

    Abstract: ... challenge. Weaning, transport, and viral infection promoted increased detection rates of M haemolytica and P ...

    Abstract Objective: To determine the efficacy of primary or booster intranasal vaccination of beef steers on clinical protection and pathogen detection following simultaneous challenge with bovine respiratory syncytial virus and bovine herpes virus 1.
    Methods: 30 beef steers were randomly allocated to 3 different treatment groups starting at 2 months of age. Group A (n = 10) was administered a single dose of a parenteral modified-live vaccine and was moved to a separate pasture. Groups B (n = 10) and C (10) remained unvaccinated. At 6 months of age, all steers were weaned and transported. Subsequently, groups A and B received a single dose of an intranasal modified-live vaccine vaccine while group C remained unvaccinated. Group C was housed separately until challenge. Two days following vaccination, all steers were challenged with bovine respiratory syncytial virus and bovine herpes virus 1 and housed in a single pen. Clinical and antibody response outcomes and the presence of nasal pathogens were evaluated.
    Results: The odds of clinical disease were lower in group A compared with group C on day 7 postchallenge; however, antibody responses and pathogen detection were not significantly different between groups before and following viral challenge. All calves remained negative for Histophilus somni and Mycoplasma bovis; however, significantly greater loads of Mannheimia haemolytica and Pasteurella multocida were detected on day 7 postchallenge compared with day -2 prechallenge.
    Clinical relevance: Intranasal booster vaccination of beef steers at 6 months of age reduced clinical disease early after viral challenge. Weaning, transport, and viral infection promoted increased detection rates of M haemolytica and P multocida regardless of vaccination status.
    MeSH term(s) Animals ; Cattle ; Herpesvirus 1, Bovine/immunology ; Male ; Administration, Intranasal/veterinary ; Respiratory Syncytial Virus, Bovine/immunology ; Immunization, Secondary/veterinary ; Coinfection/veterinary ; Coinfection/prevention & control ; Coinfection/microbiology ; Respiratory Syncytial Virus Infections/veterinary ; Respiratory Syncytial Virus Infections/prevention & control ; Infectious Bovine Rhinotracheitis/prevention & control ; Infectious Bovine Rhinotracheitis/immunology ; Cattle Diseases/prevention & control ; Cattle Diseases/microbiology ; Cattle Diseases/virology ; Cattle Diseases/immunology ; Viral Vaccines/immunology ; Viral Vaccines/administration & dosage ; Bacterial Shedding ; Antibodies, Viral/blood ; Herpesviridae Infections/veterinary ; Herpesviridae Infections/prevention & control ; Random Allocation ; Vaccination/veterinary
    Chemical Substances Viral Vaccines ; Antibodies, Viral
    Language English
    Publishing date 2024-03-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 390796-x
    ISSN 1943-5681 ; 0002-9645
    ISSN (online) 1943-5681
    ISSN 0002-9645
    DOI 10.2460/ajvr.23.11.0266
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Putative Novel Avian Paramyxovirus (AMPV) and Reidentification of APMV-2 and APMV-6 to the Species Level Based on Wild Bird Surveillance (United States, 2016-2018).

    Young, Kelsey T / Stephens, Jazz Q / Poulson, Rebecca L / Stallknecht, David E / Dimitrov, Kiril M / Butt, Salman L / Stanton, James B

    Applied and environmental microbiology

    2022  Volume 88, Issue 11, Page(s) e0046622

    Abstract: Avian paramyxoviruses (APMVs) ( ... ...

    Abstract Avian paramyxoviruses (APMVs) (subfamily
    MeSH term(s) Animals ; Animals, Wild/virology ; Avulavirus/genetics ; Avulavirus/isolation & purification ; Avulavirus Infections/epidemiology ; Avulavirus Infections/veterinary ; Avulavirus Infections/virology ; Bird Diseases/epidemiology ; Bird Diseases/virology ; Birds ; Phylogeny ; Retrospective Studies ; Sentinel Surveillance/veterinary ; United States/epidemiology
    Language English
    Publishing date 2022-05-25
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/aem.00466-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Genetic diversity of Newcastle disease viruses circulating in wild and synanthropic birds in Ukraine between 2006 and 2015.

    Goraichuk, Iryna V / Gerilovych, Anton / Bolotin, Vitaliy / Solodiankin, Olexii / Dimitrov, Kiril M / Rula, Oleksandr / Muzyka, Nataliia / Mezinov, Oleksandr / Stegniy, Borys / Kolesnyk, Olena / Pantin-Jackwood, Mary J / Miller, Patti J / Afonso, Claudio L / Muzyka, Denys

    Frontiers in veterinary science

    2023  Volume 10, Page(s) 1026296

    Abstract: Newcastle disease virus (NDV) infects a wide range of bird species worldwide and is of importance to the poultry industry. Although certain virus genotypes are clearly associated with wild bird species, the role of those species in the movement of ... ...

    Abstract Newcastle disease virus (NDV) infects a wide range of bird species worldwide and is of importance to the poultry industry. Although certain virus genotypes are clearly associated with wild bird species, the role of those species in the movement of viruses and the migratory routes they follow is still unclear. In this study, we performed a phylogenetic analysis of nineteen NDV sequences that were identified among 21,924 samples collected from wild and synanthropic birds from different regions of Ukraine from 2006 to 2015 and compared them with isolates from other continents. In synanthropic birds, NDV strains of genotype II, VI, VII, and XXI of class II were detected. The fusion gene sequences of these strains were similar to strains detected in birds from different geographical regions of Europe and Asia. However, it is noteworthy to mention the isolation of vaccine viruses from synanthropic birds, suggesting the possibility of their role in viral transmission from vaccinated poultry to wild birds, which may lead to the further spreading of vaccine viruses into other regions during wild bird migration. Moreover, here we present the first publicly available complete NDV F gene from a crow (genus
    Language English
    Publishing date 2023-01-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2023.1026296
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: A Pigeon-Derived Sub-Genotype XXI.1.2 Newcastle Disease Virus from Bangladesh Induces High Mortality in Chickens.

    Nooruzzaman, Mohammed / Barman, Lalita Rani / Mumu, Tanjin Tamanna / Chowdhury, Emdadul Haque / Dimitrov, Kiril M / Islam, Mohammad Rafiqul

    Viruses

    2021  Volume 13, Issue 8

    Abstract: Newcastle disease virus (NDV) is a significant pathogen of poultry; however, variants also affect other species, including pigeons. While NDV is endemic in Bangladesh, and poultry isolates have been recently characterized, information about viruses ... ...

    Abstract Newcastle disease virus (NDV) is a significant pathogen of poultry; however, variants also affect other species, including pigeons. While NDV is endemic in Bangladesh, and poultry isolates have been recently characterized, information about viruses infecting pigeons is limited. Worldwide, pigeon-derived isolates are commonly of low to moderate virulence for chickens. Here, we studied a pigeon-derived NDV isolated in Bangladesh in 2010. To molecularly characterize the isolate, we sequenced its complete fusion gene and performed a comprehensive phylogenetic analysis. We further studied the biological properties of the virus by estimating mean death time (MDT) and by experimentally infecting 5-week-old naïve
    MeSH term(s) Animals ; Bangladesh ; Chickens/virology ; Columbidae/virology ; Eggs/virology ; Genome, Viral ; Genotype ; Newcastle Disease/mortality ; Newcastle disease virus/classification ; Newcastle disease virus/genetics ; Newcastle disease virus/isolation & purification ; Newcastle disease virus/pathogenicity ; Phylogeny ; Poultry Diseases/mortality ; Poultry Diseases/virology ; Sequence Analysis, DNA ; Virulence
    Language English
    Publishing date 2021-08-01
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v13081520
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Genome-wide analysis reveals class and gene specific codon usage adaptation in avian paramyxoviruses 1.

    Taylor, Tonya L / Dimitrov, Kiril M / Afonso, Claudio L

    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases

    2017  Volume 50, Page(s) 28–37

    Abstract: In order to characterize the evolutionary adaptations of avian paramyxovirus 1 (APMV-1) genomes, we have compared codon usage and codon adaptation indexes among groups of Newcastle disease viruses that differ in biological, ecological, and genetic ... ...

    Abstract In order to characterize the evolutionary adaptations of avian paramyxovirus 1 (APMV-1) genomes, we have compared codon usage and codon adaptation indexes among groups of Newcastle disease viruses that differ in biological, ecological, and genetic characteristics. We have used available GenBank complete genome sequences, and compared codon usage of class I (CI-29 sequences containing 132,675 codons) and class II (CII-259 sequences containing 1,184,925 codons) APMV-1 genomes. We also compared available complete fusion protein gene sequences (CI-175 sequences containing 96,775 codons; CII-1166 sequences containing 644,798 codons). Adaptation to Gallus gallus was compared among the different classes of viruses, among different genomic regions based on transcriptional levels, or among the fusion gene. Interestingly, distinctive codon usage determined by differences in relative synonymous codon usage and by codon adaptation indexes was observed for the two APMV-1 classes and for different transcriptional regions within classes. Furthermore, differential use of the third codon position and preferential use of codon pairs were seen for the two different classes and for selected genotypes of class II despite the fact that there were no large differences in nucleotide composition. The data suggest that codon usage has changed significantly since the two APMV-1 classes diverged, however, these changes are not significantly pronounced among viruses of the same genotype, suggesting that codon adaptation in APMV-1 occurs through a slow evolutionary process.
    MeSH term(s) Adaptation, Physiological/genetics ; Animals ; Base Sequence ; Biological Evolution ; Chickens/virology ; Codon/chemistry ; Codon/metabolism ; Genome, Viral ; Genotype ; Newcastle disease virus/classification ; Newcastle disease virus/genetics ; Phylogeny ; Transcription, Genetic ; Viral Fusion Proteins/genetics
    Chemical Substances Codon ; Viral Fusion Proteins
    Language English
    Publishing date 2017-06
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2037068-4
    ISSN 1567-7257 ; 1567-1348
    ISSN (online) 1567-7257
    ISSN 1567-1348
    DOI 10.1016/j.meegid.2017.02.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Genetic and biological characterization of Newcastle disease viruses circulating in Bangladesh during 2010-2017: further genetic diversification of class II genotype XIII in Southcentral Asia.

    Nooruzzaman, Mohammed / Mumu, Tanjin Tamanna / Kabiraj, Congriev Kumar / Hasnat, Azmary / Rahman, Md Mijanur / Chowdhury, Emdadul Haque / Dimitrov, Kiril M / Islam, Mohammad Rafiqul

    The Journal of general virology

    2021  Volume 102, Issue 3

    Abstract: Newcastle disease virus (NDV) is endemic in Bangladesh and is a major threat to commercial poultry operations. While complete fusion (F) genes are recommended for molecular characterization and classification of NDV isolates, heretofore, only partial F ... ...

    Abstract Newcastle disease virus (NDV) is endemic in Bangladesh and is a major threat to commercial poultry operations. While complete fusion (F) genes are recommended for molecular characterization and classification of NDV isolates, heretofore, only partial F gene data have been available for Bangladeshi NDVs. To this end, we obtained the full-length F gene coding sequences of 11 representative NDVs isolated in Bangladesh between 2010 and 2017. In addition, one of the viruses (MK934289/chicken/Bangladesh/C161/2010) was used in an experimental infection of chickens to establish the viral pathotype and study gross and microscopic lesions. Phylogenetic analysis provided evidence that all studied Bangladeshi isolates belong to genotype XIII.2 of class II NDVs. Six of the viruses were isolated between 2010 and 2017 and grouped together with isolates from neighbouring India during 2013-2016. Another four Bangladeshi isolates (2010-2016) formed a separate monophyletic branch within XIII.2 and showed high nucleotide distance from the isolates from India and the other six Bangladeshi viruses within the sub-genotype; however, none of these groups fulfils all classification criteria to be named as a separate sub-genotype. The eleventh Bangladeshi virus studied here (C162) was genetically more distant from the remaining isolates. It out-grouped the viruses from sub-genotypes XIII.2.1 and XIII.2.2 and showed more than 9.5 % nucleotide distance from all genotype XIII sub-genotypes. This isolate may represent an NDV variant that is evolving independently from the other viruses in the region. The experimental infection in chickens revealed that the tested isolate (C161) is a velogenic viscerotropic virus. Massive haemorrhages, congestion and necrosis in different visceral organs, and lymphoid depletion in lymphoid tissues, typical for infection with velogenic NDV, were observed. Our findings demonstrate the endemic circulation of sub-genotype XIII.2 in Southcentral Asia and further genetic diversification of these viruses in Bangladesh and neighbouring India. This constant evolution of the viruses may lead to the establishment of new genetic groups in the region. Additional historical and prospective virus and surveillance data from the region and neighbouring countries will allow a more detailed epidemiological inference.
    MeSH term(s) Animals ; Asia ; Bangladesh/epidemiology ; Chickens/virology ; Evolution, Molecular ; Genetic Variation ; Genotype ; India ; Lung/pathology ; Newcastle Disease/epidemiology ; Newcastle Disease/pathology ; Newcastle Disease/virology ; Newcastle disease virus/classification ; Newcastle disease virus/genetics ; Newcastle disease virus/isolation & purification ; Newcastle disease virus/pathogenicity ; Phylogeny ; Poultry Diseases/epidemiology ; Poultry Diseases/pathology ; Poultry Diseases/virology ; RNA, Viral/genetics ; Virulence
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2021-01-28
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 219316-4
    ISSN 1465-2099 ; 0022-1317
    ISSN (online) 1465-2099
    ISSN 0022-1317
    DOI 10.1099/jgv.0.001554
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: The First Report of a Virulent Newcastle Disease Virus of Genotype VII.2 Causing Outbreaks in Chickens in Bangladesh.

    Nooruzzaman, Mohammed / Hossain, Ismail / Begum, Jahan Ara / Moula, Moktader / Khaled, Shamsul Arefin / Parvin, Rokshana / Chowdhury, Emdadul Haque / Islam, Mohammad Rafiqul / Diel, Diego G / Dimitrov, Kiril M

    Viruses

    2022  Volume 14, Issue 12

    Abstract: Newcastle disease (ND) is endemic in poultry in Bangladesh. We performed genotypic and pathotypic characterization of four ND virus (NDV) isolates from recent outbreaks in broiler chickens in Bangladesh during the period of 2020-2021. Phylogenetic ... ...

    Abstract Newcastle disease (ND) is endemic in poultry in Bangladesh. We performed genotypic and pathotypic characterization of four ND virus (NDV) isolates from recent outbreaks in broiler chickens in Bangladesh during the period of 2020-2021. Phylogenetic analysis based on the complete fusion protein gene coding sequences classified the viruses into NDV class II genotype VII.2 together with viruses from Indonesia isolated between 2014 and 2021 and a single 2020 Indian isolate. Pathogenicity testing using the intracerebral pathogenicity index in day-old chickens and mean embryo death time in embryonating chicken eggs revealed that the Bangladeshi isolates are velogenic. Inoculation of 35-day-old chickens with two NDV isolates (LT67 and N5) resulted in 100% morbidity by 3 days post inoculation (DPI), and all birds succumbed to infection by 7 DPI. Massive hemorrhages, congestion and necrotic lesions were observed in different visceral organs, which were typical for infection with a velogenic viscerotropic pathotype of NDV. At microscopic examination, tracheitis, severe pneumonia, focal proventriculitis, transmural enteritis, focal myocarditis, severe congestion and necrosis in kidneys, and lymphoid depletion in lymphoid tissues were found. Our study reports the first outbreak of the panzootic genotype VII.2 NDV in poultry in Bangladesh and documents a possible recent re-introduction of this NDV genotype from Southeast or East Asia. This study further provides viral distribution and epidemiological data that can facilitate the effective control of NDV.
    MeSH term(s) Animals ; Newcastle disease virus ; Chickens ; Phylogeny ; Bangladesh/epidemiology ; Poultry Diseases/epidemiology ; Newcastle Disease ; Genotype ; Disease Outbreaks/veterinary
    Language English
    Publishing date 2022-11-25
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14122627
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Novel Recombinant Newcastle Disease Virus-Based

    Dimitrov, Kiril M / Taylor, Tonya L / Marcano, Valerie C / Williams-Coplin, Dawn / Olivier, Timothy L / Yu, Qingzhong / Gogal, Robert M / Suarez, David L / Afonso, Claudio L

    Vaccines

    2021  Volume 9, Issue 10

    Abstract: Newcastle disease (ND) is one of the most economically important poultry diseases. Despite intensive efforts with current vaccination programs, this disease still occurs worldwide, causing significant mortality even in vaccinated flocks. This has been ... ...

    Abstract Newcastle disease (ND) is one of the most economically important poultry diseases. Despite intensive efforts with current vaccination programs, this disease still occurs worldwide, causing significant mortality even in vaccinated flocks. This has been partially attributed to a gap in immunity during the post-hatch period due to the presence of maternal antibodies that negatively impact the replication of the commonly used live vaccines.
    Language English
    Publishing date 2021-10-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines9101189
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: A Pigeon-Derived Sub-Genotype XXI.1.2 Newcastle Disease Virus from Bangladesh Induces High Mortality in Chickens

    Nooruzzaman, Mohammed / Barman, Lalita Rani / Mumu, Tanjin Tamanna / Chowdhury, Emdadul Haque / Dimitrov, Kiril M. / Islam, Mohammad Rafiqul

    Viruses. 2021 Aug. 01, v. 13, no. 8

    2021  

    Abstract: Newcastle disease virus (NDV) is a significant pathogen of poultry; however, variants also affect other species, including pigeons. While NDV is endemic in Bangladesh, and poultry isolates have been recently characterized, information about viruses ... ...

    Abstract Newcastle disease virus (NDV) is a significant pathogen of poultry; however, variants also affect other species, including pigeons. While NDV is endemic in Bangladesh, and poultry isolates have been recently characterized, information about viruses infecting pigeons is limited. Worldwide, pigeon-derived isolates are commonly of low to moderate virulence for chickens. Here, we studied a pigeon-derived NDV isolated in Bangladesh in 2010. To molecularly characterize the isolate, we sequenced its complete fusion gene and performed a comprehensive phylogenetic analysis. We further studied the biological properties of the virus by estimating mean death time (MDT) and by experimentally infecting 5-week-old naïve Sonali chickens. The studied virus clustered in sub-genotype XXI.1.2 with NDV from pigeons from Pakistan isolated during 2014–2018. Deduced amino acid sequence analysis showed a polybasic fusion protein cleavage site motif, typical for virulent NDV. The performed in vivo pathogenicity testing showed a MDT of 40.8 h, and along with previously established intracerebral pathogenicity index of 1.51, these indicated a velogenic pathotype for chickens, which is not typical for pigeon-derived viruses. The experimental infection of chickens resulted in marked neurological signs and high mortality starting at 7 days post infection (dpi). Mild congestion in the thymus and necrosis in the spleen were observed at an advanced stage of infection. Microscopically, lymphoid depletion in the thymus, spleen, and bursa of Fabricius were found at 5 dpi, which progressed to severe in the following days. Mild to moderate proliferation of glial cells was noticed in the brain starting at 2 dpi, which gradually progressed with time, leading to focal nodular aggregation. This study reports the velogenic nature for domestic chickens of a pigeon-derived NDV isolate of sub-genotype XXI.1.2. Our findings show that not all pigeon-derived viruses are of low virulence for chickens and highlight the importance of biologically evaluating the pathogenicity of NDV isolated from pigeons.
    Keywords Avian orthoavulavirus 1 ; amino acid sequences ; brain ; bursa of Fabricius ; death ; genes ; mortality ; necrosis ; pathogens ; pathotypes ; phylogeny ; sequence analysis ; spleen ; subgenotype ; virulence ; viruses ; Bangladesh ; Pakistan
    Language English
    Dates of publication 2021-0801
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v13081520
    Database NAL-Catalogue (AGRICOLA)

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