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Article ; Online: PEI-crosslinked lipase on the surface of magnetic microspheres and its characteristics.

Cao, Yi-Ping / Xia, Yu-Pei / Gu, Xiao-Fei / Han, Li / Chen, Queting / Zhi, Gao-Ying / Zhang, Dong-Hao

2020 Volume 189, Page(s) 110874

Abstract: Here, PEI@PMMA microspheres were prepared by grafting polyethyleneimine (PEI ... that PEI@PMMA microspheres had strongly adsorbed lipase (49.1 mg/g microsphere) via electrostatic ... attraction. To prevent lipase shedding, the adsorbed lipase was further crosslinked with PEI on microspheres ...

Abstract	Here, PEI@PMMA microspheres were prepared by grafting polyethyleneimine (PEI) on poly(methyl methacrylate) (PMMA) magnetic microspheres and successfully used to immobilize lipase. The results showed that PEI@PMMA microspheres had strongly adsorbed lipase (49.1 mg/g microsphere) via electrostatic attraction. To prevent lipase shedding, the adsorbed lipase was further crosslinked with PEI on microspheres using glutaraldehyde as crosslinker. Consequently, PEI-crosslinked lipase (2.14 U/mg) exhibited 2.6 times and 1.4 times higher activity respectively than the directly covalent lipase (0.82 U/mg) and the crosslinked lipase aggregates (1.57 U/mg), which was close to the activity of adsorbed lipase (2.20 U/mg). Conformational analysis from FTIR spectroscopy showed that PEI-crosslinked lipase retained its natural structure well. And the α-helix structure seemed to play a key role in enhancing lipase activity. Furthermore, the effects of various parameters on crosslinking reaction were investigated. Also, PEI-crosslinked lipase revealed higher pH and thermal stability. The Michaelis constant (K
MeSH term(s)	Adsorption ; Candida/enzymology ; Cross-Linking Reagents/chemistry ; Cross-Linking Reagents/metabolism ; Enzymes, Immobilized/chemistry ; Enzymes, Immobilized/metabolism ; Lipase/chemistry ; Lipase/metabolism ; Particle Size ; Polyethyleneimine/chemistry ; Polyethyleneimine/metabolism ; Surface Properties
Chemical Substances	Cross-Linking Reagents ; Enzymes, Immobilized ; Polyethyleneimine (9002-98-6) ; Lipase (EC 3.1.1.3)
Language	English
Publishing date	2020-02-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500523-9
ISSN	1873-4367 ; 0927-7765
ISSN (online)	1873-4367
ISSN	0927-7765
DOI	10.1016/j.colsurfb.2020.110874
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Construction of PDA-PEI/ZIF-L@PE tight ultra-filtration (TUF) membranes on porous polyethylene (PE) substrates for efficient dye/salt separation.

Li, Shiyang / Zheng, Chenchen / Tu, Longdou / Cai, Dajian / Huang, Yangxiang / Gao, Congjie / Lu, Yeqiang / Xue, Lixin

Journal of hazardous materials

2024 Volume 468, Page(s) 133727

Abstract: ... by adjusting the ligand/Zn molar ratio (α) in the preparation processes. Optimized PDA-PEI/ZIF-L@PE TUF ... frameworks micro-crystalline leaves (ZIF-L) in polyethylene imine (PEI) and polydopamine (PDA) deposit layers ... on porous polyethylene (PE) substrates. The effects of preparation conditions on the surface physical and ...

Abstract	Tight ultra-filtration (TUF) membranes were constructed by in situ growing zinc imidazole frameworks micro-crystalline leaves (ZIF-L) in polyethylene imine (PEI) and polydopamine (PDA) deposit layers on porous polyethylene (PE) substrates. The effects of preparation conditions on the surface physical and chemical structures as well as on the dye/salt separation performance of the formed TUF membranes were systematically investigated. By inserting selective water permeation channels and increasing contacting surface areas, in situ-grown ZIF-L arrays tightly cross-linked in the coating matrix greatly increased water permeation without trading off dye/salt retention selectivity. The morphology of the included ZIF-L particles could be varied by adjusting the ligand/Zn molar ratio (α) in the preparation processes. Optimized PDA-PEI/ZIF-L@PE TUF membranes containing ZIF-L of cross-cross block morphology showed very high pure water permeability of 180 ± 20 L·m
Language	English
Publishing date	2024-02-07
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1491302-1
ISSN	1873-3336 ; 0304-3894
ISSN (online)	1873-3336
ISSN	0304-3894
DOI	10.1016/j.jhazmat.2024.133727
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Article: Micromechanism Study of Molecular Compatibility of PVDF/PEI Blend Membrane.

Gao, Ming / Zhu, Yuanlu / Yan, Jiangyi / Wu, Weixing / Wang, Beifu

Membranes

2022 Volume 12, Issue 8

Abstract: In this paper, the compatibility of polyetherimide (PEI) with different contents as a high ... performance copolymer and polyvinylidene fluoride (PVDF) was studied, and 5%-20% PEI was prepared by the non ... solvent-induced phase inversion method. The compatibility of PVDF and PEI was evaluated by analyzing ...

Abstract	In this paper, the compatibility of polyetherimide (PEI) with different contents as a high-performance copolymer and polyvinylidene fluoride (PVDF) was studied, and 5%-20% PEI was prepared by the non-solvent-induced phase inversion method. The compatibility of PVDF and PEI was evaluated by analyzing the physical structure and properties of the blend membrane, the microstructure, the glass transition temperature Tg, the enthalpy, and the mechanism of the polymer blend enthalpy change. The results show that the blend membranes have -NH and C=O-N binding energies at X-ray photoelectron spectroscopy (XPS), which preliminarily proves that fluorine-amine bonds are formed between the polymers, and new spectra appeared by Fourier transform infrared (FTIR) and X-ray diffraction (XRD) peaks, which further proves that the two have the formation of fluorine-amine bonds, the Tg and enthalpy of the mixed membrane was increased, and a scanning electron microscope (SEM) observed that the membrane pores changed from finger-like pores to sponge-like macropores. When the content of PEI is 15%, the performance of the blended membrane is the best, the water contact angle increases to 58.5°, the porosity increases to 17.33%, the maximum force increases to 8.04 N, and the elongation at break decreases to 24.26%, the pure water flux is 1870.292 L/m
Language	English
Publishing date	2022-08-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2614641-1
ISSN	2077-0375
ISSN	2077-0375
DOI	10.3390/membranes12080809
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Article ; Online: Molecular Compatibility and Hydrogen Bonding Mechanism of PES/PEI Blends.

Zhu, Yuanlu / Wu, Weixing / Gao, Ming / Yan, Jiangyi / Wang, Beifu

Polymers

2022 Volume 14, Issue 15

Abstract: The development of high-performance polymer membranes has sparked a lot of attention in recent years. Polymer blending is a potential method of modification. A limitation, however, is the compatibility of blends at the molecular level. In this ... ...

Abstract	The development of high-performance polymer membranes has sparked a lot of attention in recent years. Polymer blending is a potential method of modification. A limitation, however, is the compatibility of blends at the molecular level. In this investigation, polyethersulfone/polyetherimide hollow fiber membranes were prepared by the solution blending method. Compatibility, hydrogen bonding, crystallinity, microstructure, hydrophilicity, mechanical properties, and transmissibility of blended membranes were also characterized. The compatibility and hydrogen bonding action of the two components were confirmed by DSC, FTIR, XPS, and XRD. The structure exhibits a C-H···O interaction motif with the sulfone group acting as a hydrogen bond acceptor from a methyl C-H donor. The π-π stacking between the two polymers arranged molecules more orderly, resulting in enhanced intermolecular interactions. Compared to polyethersulfone hollow fiber membranes, the hydrophilic, mechanical properties, and rejection rate of the blended membranes are more effectively enhanced. Self-assembly of the host polymer with a polymer capable of forming hydrogen bonds to construct controllable blends is a crucial and proven method.
Language	English
Publishing date	2022-07-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527146-5
ISSN	2073-4360 ; 2073-4360
ISSN (online)	2073-4360
ISSN	2073-4360
DOI	10.3390/polym14153046
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Article ; Online: Graphene Oxide/Styrene-Butadiene Latex Hybrid Aerogel with Improved Mechanical Properties by PEI Grafted GO and CNT.

Zhao, Zetian / Zhang, Lina / Song, Yinghu / Ma, Lichun / Li, Jialiang / Zhao, Min / Ji, Xueliang / Gao, Jianfei / Song, Guojun / Li, Xiaoru

Gels (Basel, Switzerland)

2023 Volume 9, Issue 5

Abstract: ... applications. In this study, polyethyleneimide (PEI) was used to graft onto the surface of GO and ... butadiene latex (SBL) to the modified GO and CNTs. The synergistic effect of PEI and SBL, resulted ... the best, and the maximum compressive stress was 784.35% higher than that of GOA. The graft of PEI ...

Abstract	Graphene oxide aerogel (GOA) has wide application prospects due to its low density and high porosity. However, the poor mechanical properties and unstable structure of GOA have limited its practical applications. In this study, polyethyleneimide (PEI) was used to graft onto the surface of GO and carbon nanotubes (CNTs) to improve compatibility with polymers. Composite GOA was prepared by adding styrene-butadiene latex (SBL) to the modified GO and CNTs. The synergistic effect of PEI and SBL, resulted in an aerogel with excellent mechanical properties, compressive resistance, and structural stability. When the ratio of SBL to GO and GO to CNTs was 2:1 and 7:3, respectively, the obtained aerogel performance was the best, and the maximum compressive stress was 784.35% higher than that of GOA. The graft of PEI on the surface of GO and CNT could improve the mechanical properties of the aerogel, with greater improvements observed with grafting onto the surface of GO. Compared with GO/CNT/SBL aerogel without PEI grafting, the maximum stress of GO/CNT-PEI/SBL aerogel increased by 5.57%, that of GO-PEI/CNT/SBL aerogel increased by 20.25%, and that of GO-PEI/CNT-PEI/SBL aerogel increased by 28.99%. This work not only provided a possibility for the practical application of aerogel, but also steered the research of GOA in a new direction.
Language	English
Publishing date	2023-05-16
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2813982-3
ISSN	2310-2861 ; 2310-2861
ISSN (online)	2310-2861
ISSN	2310-2861
DOI	10.3390/gels9050419
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Dual signal amplified electrochemical aptasensor based on PEI-functionalized GO and ROP for highly sensitive detection of cTnI.

Zhou, Zhenbo / Gao, Tianyu / Zhao, Yuning / Yang, Peng / Cheng, Di / Yang, Huaixia / Wang, Ying / Li, Xiaofei

Bioelectrochemistry (Amsterdam, Netherlands)

2023 Volume 151, Page(s) 108402

Abstract: ... assembly of Au-S bonds to specifically capture cTnI. After specific recognition of cTnI, Apt2 coated PEI ... surface. The combined application of PEI-functionalized GO and NCA-Fc better achieves the high sensitivity ...

Abstract	Cardiac troponin I (cTnI) is considered as the gold standard for the diagnosis of acute myocardial infarction (AMI) because of its excellent specificity and sensitivity. Herein, a novel aptasensor based on the dual signal amplification strategy of Polyethyleneimine functionalized Graphene oxide (GO) and ring-opening polymerization (ROP) for the first time was successfully constructed to achieve high sensitivity detection of cTnI. Briefly, cTnI-aptamer 1 (Apt1) was immobilized on the surface of gold electrode by self-assembly of Au-S bonds to specifically capture cTnI. After specific recognition of cTnI, Apt2 coated PEI-functionalized GO composites acted as macroinitiators for the subsequent ROP reaction. Next, α-amino acid-N-carboxylic acid anhydride ferrocene derivatives (NCA-Fc), the monomer for ROP reaction, was added to the electrode surface. The combined application of PEI-functionalized GO and NCA-Fc better achieves the high sensitivity and signal amplification of the aptasensor. Under optimal conditions, the aptasensor exhibited a wide linear range of 10 fg mL
MeSH term(s)	Limit of Detection ; Troponin I/analysis ; Polymerization ; Aptamers, Nucleotide/chemistry ; Graphite/chemistry ; Electrochemical Techniques/methods ; Biosensing Techniques/methods ; Metal Nanoparticles/chemistry
Chemical Substances	graphene oxide ; Troponin I ; Aptamers, Nucleotide ; Graphite (7782-42-5)
Language	English
Publishing date	2023-02-20
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2010650-6
ISSN	1878-562X ; 0302-4598 ; 1567-5394
ISSN (online)	1878-562X
ISSN	0302-4598 ; 1567-5394
DOI	10.1016/j.bioelechem.2023.108402
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Article: PEI-crosslinked lipase on the surface of magnetic microspheres and its characteristics

Cao, Yi-Ping / Xia, Yu-Pei / Gu, Xiao-Fei / Han, Li / Chen, Queting / Zhi, Gao-Ying / Zhang, Dong-Hao

Colloids and surfaces. 2020 May, v. 189

2020

Abstract: Here, PEI@PMMA microspheres were prepared by grafting polyethyleneimine (PEI ... that PEI@PMMA microspheres had strongly adsorbed lipase (49.1 mg/g microsphere) via electrostatic ... attraction. To prevent lipase shedding, the adsorbed lipase was further crosslinked with PEI on microspheres ...

Abstract	Here, PEI@PMMA microspheres were prepared by grafting polyethyleneimine (PEI) on poly(methyl methacrylate) (PMMA) magnetic microspheres and successfully used to immobilize lipase. The results showed that PEI@PMMA microspheres had strongly adsorbed lipase (49.1 mg/g microsphere) via electrostatic attraction. To prevent lipase shedding, the adsorbed lipase was further crosslinked with PEI on microspheres using glutaraldehyde as crosslinker. Consequently, PEI-crosslinked lipase (2.14 U/mg) exhibited 2.6 times and 1.4 times higher activity respectively than the directly covalent lipase (0.82 U/mg) and the crosslinked lipase aggregates (1.57 U/mg), which was close to the activity of adsorbed lipase (2.20 U/mg). Conformational analysis from FTIR spectroscopy showed that PEI-crosslinked lipase retained its natural structure well. And the α-helix structure seemed to play a key role in enhancing lipase activity. Furthermore, the effects of various parameters on crosslinking reaction were investigated. Also, PEI-crosslinked lipase revealed higher pH and thermal stability. The Michaelis constant (Km) was increased and the optimum temperature of lipase was widened observably after crosslinking with PEI on PEI@PMMA magnetic microspheres.
Keywords	Fourier transform infrared spectroscopy ; carboxylic ester hydrolases ; crosslinking ; electrostatic interactions ; enzyme activity ; enzyme kinetics ; glutaraldehyde ; magnetism ; microparticles ; molecular conformation ; pH ; polyethyleneimine ; polymethylmethacrylate ; temperature ; thermal stability
Language	English
Dates of publication	2020-05
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1500523-9
ISSN	1873-4367 ; 0927-7765
ISSN (online)	1873-4367
ISSN	0927-7765
DOI	10.1016/j.colsurfb.2020.110874
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: A PEI/TMC membrane modified with an ionic liquid with enhanced permeability and antibacterial properties for the removal of heavy metal ions.

Zhang, Xin / Zheng, Junfeng / Jin, Pengrui / Xu, Daliang / Yuan, Shushan / Zhao, Rui / Depuydt, Stef / Gao, Yujie / Xu, Zhen-Liang / Van der Bruggen, Bart

Journal of hazardous materials

2022 Volume 435, Page(s) 129010

Abstract: ... In this study, a positively charged THPC/PEI-TMC NF membrane was designed via simple one-step incorporation ... of Tetrakis (hydroxymethyl) phosphonium chloride (THPC) biocide on the surface of PEI-TMC membranes ... significantly optimizing surface morphology, roughness, hydrophilicity, and zeta potential of PEI-TMC membranes ...

Abstract	Heavy metal ions in drinking water severely threaten public health in various places worldwide. Nanofiltration (NF) membrane technology is an attractive option for heavy metal ions removal; however, improving NF membrane filtration performance is required to make their industrial application viable. In this study, a positively charged THPC/PEI-TMC NF membrane was designed via simple one-step incorporation of Tetrakis (hydroxymethyl) phosphonium chloride (THPC) biocide on the surface of PEI-TMC membranes, significantly optimizing surface morphology, roughness, hydrophilicity, and zeta potential of PEI-TMC membranes. It was found that the pure water permeability (11.6 Lm
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Ionic Liquids ; Ions ; Membranes, Artificial ; Metals, Heavy ; Permeability
Chemical Substances	Anti-Bacterial Agents ; Ionic Liquids ; Ions ; Membranes, Artificial ; Metals, Heavy
Language	English
Publishing date	2022-04-27
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1491302-1
ISSN	1873-3336 ; 0304-3894
ISSN (online)	1873-3336
ISSN	0304-3894
DOI	10.1016/j.jhazmat.2022.129010
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Article ; Online: Molecular Compatibility and Hydrogen Bonding Mechanism of PES/PEI Blends

Yuanlu Zhu / Weixing Wu / Ming Gao / Jiangyi Yan / Beifu Wang

Polymers, Vol 14, Iss 3046, p

2022 Volume 3046

Abstract	The development of high-performance polymer membranes has sparked a lot of attention in recent years. Polymer blending is a potential method of modification. A limitation, however, is the compatibility of blends at the molecular level. In this investigation, polyethersulfone/polyetherimide hollow fiber membranes were prepared by the solution blending method. Compatibility, hydrogen bonding, crystallinity, microstructure, hydrophilicity, mechanical properties, and transmissibility of blended membranes were also characterized. The compatibility and hydrogen bonding action of the two components were confirmed by DSC, FTIR, XPS, and XRD. The structure exhibits a C−H···O interaction motif with the sulfone group acting as a hydrogen bond acceptor from a methyl C−H donor. The π–π stacking between the two polymers arranged molecules more orderly, resulting in enhanced intermolecular interactions. Compared to polyethersulfone hollow fiber membranes, the hydrophilic, mechanical properties, and rejection rate of the blended membranes are more effectively enhanced. Self-assembly of the host polymer with a polymer capable of forming hydrogen bonds to construct controllable blends is a crucial and proven method.
Keywords	polyethersulfone ; polyetherimide ; blend ; hydrogen bonding ; compatibility ; Organic chemistry ; QD241-441
Subject code	540
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Authors Reply to Letter to the Editor - In Response To: Comment on Pei M et al.'s "Analysis of Parafoveal Microvascular Abnormalities in Behcet's Uveitis Using Projection-resolved Optical Coherence Tomographic Angiography".

Pei, Minghang / Zhao, Chan / Gao, Fei / Qu, Yi / Liang, Anyi / Xiao, Junyan / Zhang, Meifen

Ocular immunology and inflammation

2020 Volume 30, Issue 2, Page(s) 522–523

MeSH term(s)	Angiography ; Behcet Syndrome/complications ; Behcet Syndrome/diagnosis ; Fluorescein Angiography ; Humans ; Tomography, Optical Coherence/methods ; Uveitis/diagnosis ; Uveitis/etiology
Language	English
Publishing date	2020-09-18
Publishing country	England
Document type	Journal Article ; Comment
ZDB-ID	1193873-0
ISSN	1744-5078 ; 0927-3948
ISSN (online)	1744-5078
ISSN	0927-3948
DOI	10.1080/09273948.2020.1807201
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